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Q07879 (ATG10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-like-conjugating enzyme ATG10

EC=6.3.2.-
Alternative name(s):
Autophagy-related protein 10
Gene names
Name:ATG10
Synonyms:APG10
Ordered Locus Names:YLL042C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length167 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E2-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for proper localization of ATG8 to the preautophagosomal structure (PAS). Likely serves as an ATG5-recognition molecule. Ref.4 Ref.5 Ref.6 Ref.7 Ref.12 Ref.13 Ref.17

Subunit structure

Forms homooligomers. Interacts with ATG7 and ATG12. Ref.6 Ref.9 Ref.14 Ref.15 Ref.16 Ref.18

Subcellular location

Preautophagosomal structure membrane; Peripheral membrane protein Ref.11.

Sequence similarities

Belongs to the ATG10 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 167167Ubiquitin-like-conjugating enzyme ATG10
PRO_0000096190

Sites

Active site1331Glycyl thioester intermediate

Experimental info

Mutagenesis261C → S: No effect on conjugating activity. Normal autophagic activity. Ref.6
Mutagenesis1331C → A: Complete loss of covalent binding to ATG12. Ref.6
Mutagenesis1331C → S: Strong decrease of binding to ATG12. No more formation of ATG12-ATG5 conjugate. Defect in autophagy. Ref.6
Mutagenesis1371C → S: No effect on conjugating activity. Normal autophagic activity. Ref.6

Secondary structure

................................ 167
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q07879 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9B90684B8D3D26C5

FASTA16719,760
        10         20         30         40         50         60 
MIPYQEWHSQ LQSLYDSQIF HNWALCQDVH LNDEKDGLLL RLIPTRQLQK NTERIENKLL 

        70         80         90        100        110        120 
NHIELYLTYS KVYNEPLLLL RIWEEKSIDG IPMTKLMLPT DIESLLDVQG KFQLGLDTII 

       130        140        150        160 
NLEGSVWYSF HPCDTSCIVG DQAEFMSTYL RRWVSIFIFS WLGYEDS 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A. expand/collapse author list , Gonzaga L., Vasconcelos A.T., Simpson A.J.G., Kolodner R., Harlow E., LaBaer J.
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
Tsukada M., Ohsumi Y.
FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"A protein conjugation system essential for autophagy."
Mizushima N., Noda T., Yoshimori T., Tanaka Y., Ishii T., George M.D., Klionsky D.J., Ohsumi M., Ohsumi Y.
Nature 395:395-398(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast."
Shintani T., Mizushima N., Ogawa Y., Matsuura A., Noda T., Ohsumi Y.
EMBO J. 18:5234-5241(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ATG7 AND ATG12, MUTAGENESIS OF CYS-26; CYS-133 AND CYS-137.
[7]"The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"A unified nomenclature for yeast autophagy-related genes."
Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.
Dev. Cell 5:539-545(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[9]"Structure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy."
Hanada T., Ohsumi Y.
Autophagy 1:110-118(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG12.
[10]"Crystallization and preliminary X-ray analysis of Atg10."
Yamaguti M., Suzuki N.N., Fujioka Y., Ohsumi Y., Inagaki F.
Acta Crystallogr. F 63:443-445(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CRYSTALLIZATION.
[11]"Hierarchy of Atg proteins in pre-autophagosomal structure organization."
Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[12]"In vivo reconstitution of autophagy in Saccharomyces cerevisiae."
Cao Y., Cheong H., Song H., Klionsky D.J.
J. Cell Biol. 182:703-713(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Piecemeal microautophagy of the nucleus requires the core macroautophagy genes."
Krick R., Muehe Y., Prick T., Bremer S., Schlotterhose P., Eskelinen E.L., Millen J., Goldfarb D.S., Thumm M.
Mol. Biol. Cell 19:4492-4505(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"Purification and characterization of a ubiquitin-like system for autophagosome formation."
Bae J.Y., Park H.H.
J. Microbiol. Biotechnol. 20:1647-1652(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[15]"Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures."
Kaiser S.E., Mao K., Taherbhoy A.M., Yu S., Olszewski J.L., Duda D.M., Kurinov I., Deng A., Fenn T.D., Klionsky D.J., Schulman B.A.
Nat. Struct. Mol. Biol. 19:1242-1249(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG7.
[16]"Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8."
Hong S.B., Kim B.W., Lee K.E., Kim S.W., Jeon H., Kim J., Song H.K.
Nat. Struct. Mol. Biol. 18:1323-1330(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG7.
[17]"A late form of nucleophagy in Saccharomyces cerevisiae."
Mijaljica D., Prescott M., Devenish R.J.
PLoS ONE 7:E40013-E40013(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[18]"Structure of the autophagic E2 enzyme Atg10."
Hong S.B., Kim B.W., Kim J.H., Song H.K.
Acta Crystallogr. D 68:1409-1417(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), INTERACTION WITH ATG7.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73147 Genomic DNA. Translation: CAA97493.1.
AY558190 Genomic DNA. Translation: AAS56516.1.
BK006945 Genomic DNA. Translation: DAA09280.1.
PIRS64794.
RefSeqNP_013058.1. NM_001181862.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EBRX-ray2.70A/B1-167[»]
4GSKX-ray2.90Y/Z1-167[»]
ProteinModelPortalQ07879.
SMRQ07879. Positions 1-165.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31271. 38 interactions.
DIPDIP-4842N.
IntActQ07879. 2 interactions.
MINTMINT-487830.
STRING4932.YLL042C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLL042C; YLL042C; YLL042C.
GeneID850684.
KEGGsce:YLL042C.

Organism-specific databases

CYGDYLL042c.
SGDS000003965. ATG10.

Phylogenomic databases

eggNOGNOG70099.
KOK08338.
OMARISIAIT.
OrthoDBEOG7FBRXB.

Enzyme and pathway databases

BioCycYEAST:G3O-32143-MONOMER.

Gene expression databases

GenevestigatorQ07879.

Family and domain databases

InterProIPR016524. Autophagy-rel_prot_10.
IPR007135. Autophagy-rel_prot_3.
[Graphical view]
PfamPF03987. Autophagy_act_C. 1 hit.
[Graphical view]
PIRSFPIRSF007802. Autophagy-rel_ATG10. 1 hit.
ProtoNetSearch...

Other

NextBio966689.

Entry information

Entry nameATG10_YEAST
AccessionPrimary (citable) accession number: Q07879
Secondary accession number(s): D6VXW4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references