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Protein

Ubiquitin-like-conjugating enzyme ATG10

Gene

ATG10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E2-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for proper localization of ATG8 to the preautophagosomal structure (PAS). Likely serves as an ATG5-recognition molecule.7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei133Glycyl thioester intermediate1

GO - Molecular functioni

  • Atg12 transferase activity Source: SGD

GO - Biological processi

  • autophagy of mitochondrion Source: SGD
  • cellular protein modification process Source: SGD
  • late nucleophagy Source: SGD
  • macroautophagy Source: SGD
  • piecemeal microautophagy of the nucleus Source: SGD
  • protein localization by the Cvt pathway Source: SGD
  • protein transport Source: UniProtKB-KW

Keywordsi

Molecular functionTransferase
Biological processAutophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32143-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like-conjugating enzyme ATG10 (EC:2.3.2.-)
Alternative name(s):
Autophagy-related protein 10
Gene namesi
Name:ATG10
Synonyms:APG10
Ordered Locus Names:YLL042C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL042C
SGDiS000003965 ATG10

Subcellular locationi

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26C → S: No effect on conjugating activity. Normal autophagic activity. 1 Publication1
Mutagenesisi133C → A: Complete loss of covalent binding to ATG12. 1 Publication1
Mutagenesisi133C → S: Strong decrease of binding to ATG12. No more formation of ATG12-ATG5 conjugate. Defect in autophagy. 1 Publication1
Mutagenesisi137C → S: No effect on conjugating activity. Normal autophagic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000961901 – 167Ubiquitin-like-conjugating enzyme ATG10Add BLAST167

Proteomic databases

PaxDbiQ07879

Interactioni

Subunit structurei

Forms homooligomers. Interacts with ATG7 and ATG12.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG7P388627EBI-36629,EBI-2677

Protein-protein interaction databases

BioGridi31271, 134 interactors
DIPiDIP-4842N
IntActiQ07879, 3 interactors
STRINGi4932.YLL042C

Structurei

Secondary structure

1167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 16Combined sources13
Turni17 – 22Combined sources6
Beta strandi26 – 31Combined sources6
Beta strandi33 – 36Combined sources4
Beta strandi38 – 42Combined sources5
Helixi46 – 54Combined sources9
Beta strandi61 – 70Combined sources10
Turni71 – 74Combined sources4
Beta strandi75 – 88Combined sources14
Beta strandi91 – 96Combined sources6
Helixi99 – 106Combined sources8
Turni107 – 110Combined sources4
Beta strandi115 – 122Combined sources8
Beta strandi125 – 129Combined sources5
Helixi135 – 137Combined sources3
Helixi146 – 149Combined sources4
Helixi150 – 157Combined sources8
Helixi159 – 162Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EBRX-ray2.70A/B1-167[»]
4GSKX-ray2.90Y/Z1-167[»]
ProteinModelPortaliQ07879
SMRiQ07879
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG10 family.Curated

Phylogenomic databases

InParanoidiQ07879
KOiK08338
OMAiNISHLEC
OrthoDBiEOG092C55IO

Family and domain databases

InterProiView protein in InterPro
IPR016524 Atg10
IPR007135 Autophagy-rel_prot_3
PfamiView protein in Pfam
PF03987 Autophagy_act_C, 1 hit
PIRSFiPIRSF007802 Autophagy-rel_ATG10, 1 hit

Sequencei

Sequence statusi: Complete.

Q07879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPYQEWHSQ LQSLYDSQIF HNWALCQDVH LNDEKDGLLL RLIPTRQLQK
60 70 80 90 100
NTERIENKLL NHIELYLTYS KVYNEPLLLL RIWEEKSIDG IPMTKLMLPT
110 120 130 140 150
DIESLLDVQG KFQLGLDTII NLEGSVWYSF HPCDTSCIVG DQAEFMSTYL
160
RRWVSIFIFS WLGYEDS
Length:167
Mass (Da):19,760
Last modified:November 1, 1996 - v1
Checksum:i9B90684B8D3D26C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73147 Genomic DNA Translation: CAA97493.1
AY558190 Genomic DNA Translation: AAS56516.1
BK006945 Genomic DNA Translation: DAA09280.1
PIRiS64794
RefSeqiNP_013058.1, NM_001181862.1

Genome annotation databases

EnsemblFungiiYLL042C; YLL042C; YLL042C
GeneIDi850684
KEGGisce:YLL042C

Similar proteinsi

Entry informationi

Entry nameiATG10_YEAST
AccessioniPrimary (citable) accession number: Q07879
Secondary accession number(s): D6VXW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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