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Q07879

- ATG10_YEAST

UniProt

Q07879 - ATG10_YEAST

Protein

Ubiquitin-like-conjugating enzyme ATG10

Gene

ATG10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    E2-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for proper localization of ATG8 to the preautophagosomal structure (PAS). Likely serves as an ATG5-recognition molecule.7 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei133 – 1331Glycyl thioester intermediate

    GO - Molecular functioni

    1. Atg12 ligase activity Source: SGD

    GO - Biological processi

    1. cellular protein modification process Source: SGD
    2. CVT pathway Source: SGD
    3. late nucleophagy Source: SGD
    4. macroautophagy Source: SGD
    5. mitochondrion degradation Source: SGD
    6. piecemeal microautophagy of nucleus Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Autophagy, Protein transport, Transport, Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32143-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-like-conjugating enzyme ATG10 (EC:6.3.2.-)
    Alternative name(s):
    Autophagy-related protein 10
    Gene namesi
    Name:ATG10
    Synonyms:APG10
    Ordered Locus Names:YLL042C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLL042c.
    SGDiS000003965. ATG10.

    Subcellular locationi

    Preautophagosomal structure membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. pre-autophagosomal structure membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi26 – 261C → S: No effect on conjugating activity. Normal autophagic activity. 1 Publication
    Mutagenesisi133 – 1331C → A: Complete loss of covalent binding to ATG12. 1 Publication
    Mutagenesisi133 – 1331C → S: Strong decrease of binding to ATG12. No more formation of ATG12-ATG5 conjugate. Defect in autophagy. 1 Publication
    Mutagenesisi137 – 1371C → S: No effect on conjugating activity. Normal autophagic activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 167167Ubiquitin-like-conjugating enzyme ATG10PRO_0000096190Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriQ07879.

    Interactioni

    Subunit structurei

    Forms homooligomers. Interacts with ATG7 and ATG12.6 Publications

    Protein-protein interaction databases

    BioGridi31271. 38 interactions.
    DIPiDIP-4842N.
    IntActiQ07879. 2 interactions.
    MINTiMINT-487830.
    STRINGi4932.YLL042C.

    Structurei

    Secondary structure

    1
    167
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1613
    Turni17 – 226
    Beta strandi26 – 316
    Beta strandi33 – 364
    Beta strandi38 – 425
    Helixi46 – 549
    Beta strandi61 – 7010
    Turni71 – 744
    Beta strandi75 – 8814
    Beta strandi91 – 966
    Helixi99 – 1068
    Turni107 – 1104
    Beta strandi115 – 1228
    Beta strandi125 – 1295
    Helixi135 – 1373
    Helixi146 – 1494
    Helixi150 – 1578
    Helixi159 – 1624

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4EBRX-ray2.70A/B1-167[»]
    4GSKX-ray2.90Y/Z1-167[»]
    ProteinModelPortaliQ07879.
    SMRiQ07879. Positions 1-165.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATG10 family.Curated

    Phylogenomic databases

    eggNOGiNOG70099.
    KOiK08338.
    OMAiRISIAIT.
    OrthoDBiEOG7FBRXB.

    Family and domain databases

    InterProiIPR016524. Autophagy-rel_prot_10.
    IPR007135. Autophagy-rel_prot_3.
    [Graphical view]
    PfamiPF03987. Autophagy_act_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007802. Autophagy-rel_ATG10. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q07879-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIPYQEWHSQ LQSLYDSQIF HNWALCQDVH LNDEKDGLLL RLIPTRQLQK    50
    NTERIENKLL NHIELYLTYS KVYNEPLLLL RIWEEKSIDG IPMTKLMLPT 100
    DIESLLDVQG KFQLGLDTII NLEGSVWYSF HPCDTSCIVG DQAEFMSTYL 150
    RRWVSIFIFS WLGYEDS 167
    Length:167
    Mass (Da):19,760
    Last modified:November 1, 1996 - v1
    Checksum:i9B90684B8D3D26C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73147 Genomic DNA. Translation: CAA97493.1.
    AY558190 Genomic DNA. Translation: AAS56516.1.
    BK006945 Genomic DNA. Translation: DAA09280.1.
    PIRiS64794.
    RefSeqiNP_013058.1. NM_001181862.1.

    Genome annotation databases

    EnsemblFungiiYLL042C; YLL042C; YLL042C.
    GeneIDi850684.
    KEGGisce:YLL042C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73147 Genomic DNA. Translation: CAA97493.1 .
    AY558190 Genomic DNA. Translation: AAS56516.1 .
    BK006945 Genomic DNA. Translation: DAA09280.1 .
    PIRi S64794.
    RefSeqi NP_013058.1. NM_001181862.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4EBR X-ray 2.70 A/B 1-167 [» ]
    4GSK X-ray 2.90 Y/Z 1-167 [» ]
    ProteinModelPortali Q07879.
    SMRi Q07879. Positions 1-165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31271. 38 interactions.
    DIPi DIP-4842N.
    IntActi Q07879. 2 interactions.
    MINTi MINT-487830.
    STRINGi 4932.YLL042C.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLL042C ; YLL042C ; YLL042C .
    GeneIDi 850684.
    KEGGi sce:YLL042C.

    Organism-specific databases

    CYGDi YLL042c.
    SGDi S000003965. ATG10.

    Phylogenomic databases

    eggNOGi NOG70099.
    KOi K08338.
    OMAi RISIAIT.
    OrthoDBi EOG7FBRXB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32143-MONOMER.

    Miscellaneous databases

    NextBioi 966689.

    Gene expression databases

    Genevestigatori Q07879.

    Family and domain databases

    InterProi IPR016524. Autophagy-rel_prot_10.
    IPR007135. Autophagy-rel_prot_3.
    [Graphical view ]
    Pfami PF03987. Autophagy_act_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF007802. Autophagy-rel_ATG10. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
      Tsukada M., Ohsumi Y.
      FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: FUNCTION.
    6. "Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast."
      Shintani T., Mizushima N., Ogawa Y., Matsuura A., Noda T., Ohsumi Y.
      EMBO J. 18:5234-5241(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATG7 AND ATG12, MUTAGENESIS OF CYS-26; CYS-133 AND CYS-137.
    7. "The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
      Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
      EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: NOMENCLATURE.
    9. "Structure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy."
      Hanada T., Ohsumi Y.
      Autophagy 1:110-118(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG12.
    10. "Crystallization and preliminary X-ray analysis of Atg10."
      Yamaguti M., Suzuki N.N., Fujioka Y., Ohsumi Y., Inagaki F.
      Acta Crystallogr. F 63:443-445(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
    11. "Hierarchy of Atg proteins in pre-autophagosomal structure organization."
      Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
      Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    12. "In vivo reconstitution of autophagy in Saccharomyces cerevisiae."
      Cao Y., Cheong H., Song H., Klionsky D.J.
      J. Cell Biol. 182:703-713(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Piecemeal microautophagy of the nucleus requires the core macroautophagy genes."
      Krick R., Muehe Y., Prick T., Bremer S., Schlotterhose P., Eskelinen E.L., Millen J., Goldfarb D.S., Thumm M.
      Mol. Biol. Cell 19:4492-4505(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Purification and characterization of a ubiquitin-like system for autophagosome formation."
      Bae J.Y., Park H.H.
      J. Microbiol. Biotechnol. 20:1647-1652(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    15. "Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures."
      Kaiser S.E., Mao K., Taherbhoy A.M., Yu S., Olszewski J.L., Duda D.M., Kurinov I., Deng A., Fenn T.D., Klionsky D.J., Schulman B.A.
      Nat. Struct. Mol. Biol. 19:1242-1249(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG7.
    16. "Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8."
      Hong S.B., Kim B.W., Lee K.E., Kim S.W., Jeon H., Kim J., Song H.K.
      Nat. Struct. Mol. Biol. 18:1323-1330(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG7.
    17. "A late form of nucleophagy in Saccharomyces cerevisiae."
      Mijaljica D., Prescott M., Devenish R.J.
      PLoS ONE 7:E40013-E40013(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    18. Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), INTERACTION WITH ATG7.

    Entry informationi

    Entry nameiATG10_YEAST
    AccessioniPrimary (citable) accession number: Q07879
    Secondary accession number(s): D6VXW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3