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Q07879

- ATG10_YEAST

UniProt

Q07879 - ATG10_YEAST

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Protein

Ubiquitin-like-conjugating enzyme ATG10

Gene

ATG10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

E2-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for proper localization of ATG8 to the preautophagosomal structure (PAS). Likely serves as an ATG5-recognition molecule.7 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei133 – 1331Glycyl thioester intermediate

GO - Molecular functioni

  1. Atg12 ligase activity Source: SGD

GO - Biological processi

  1. cellular protein modification process Source: SGD
  2. CVT pathway Source: SGD
  3. late nucleophagy Source: SGD
  4. macroautophagy Source: SGD
  5. mitochondrion degradation Source: SGD
  6. piecemeal microautophagy of nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Autophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32143-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like-conjugating enzyme ATG10 (EC:6.3.2.-)
Alternative name(s):
Autophagy-related protein 10
Gene namesi
Name:ATG10
Synonyms:APG10
Ordered Locus Names:YLL042C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLL042c.
SGDiS000003965. ATG10.

Subcellular locationi

Preautophagosomal structure membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: InterPro
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi26 – 261C → S: No effect on conjugating activity. Normal autophagic activity. 1 Publication
Mutagenesisi133 – 1331C → A: Complete loss of covalent binding to ATG12. 1 Publication
Mutagenesisi133 – 1331C → S: Strong decrease of binding to ATG12. No more formation of ATG12-ATG5 conjugate. Defect in autophagy. 1 Publication
Mutagenesisi137 – 1371C → S: No effect on conjugating activity. Normal autophagic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 167167Ubiquitin-like-conjugating enzyme ATG10PRO_0000096190Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriQ07879.

Interactioni

Subunit structurei

Forms homooligomers. Interacts with ATG7 and ATG12.6 Publications

Protein-protein interaction databases

BioGridi31271. 38 interactions.
DIPiDIP-4842N.
IntActiQ07879. 2 interactions.
MINTiMINT-487830.
STRINGi4932.YLL042C.

Structurei

Secondary structure

1
167
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1613
Turni17 – 226
Beta strandi26 – 316
Beta strandi33 – 364
Beta strandi38 – 425
Helixi46 – 549
Beta strandi61 – 7010
Turni71 – 744
Beta strandi75 – 8814
Beta strandi91 – 966
Helixi99 – 1068
Turni107 – 1104
Beta strandi115 – 1228
Beta strandi125 – 1295
Helixi135 – 1373
Helixi146 – 1494
Helixi150 – 1578
Helixi159 – 1624

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EBRX-ray2.70A/B1-167[»]
4GSKX-ray2.90Y/Z1-167[»]
ProteinModelPortaliQ07879.
SMRiQ07879. Positions 1-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG10 family.Curated

Phylogenomic databases

eggNOGiNOG70099.
InParanoidiQ07879.
KOiK08338.
OMAiRISIAIT.
OrthoDBiEOG7FBRXB.

Family and domain databases

InterProiIPR016524. Autophagy-rel_prot_10.
IPR007135. Autophagy-rel_prot_3.
[Graphical view]
PfamiPF03987. Autophagy_act_C. 1 hit.
[Graphical view]
PIRSFiPIRSF007802. Autophagy-rel_ATG10. 1 hit.

Sequencei

Sequence statusi: Complete.

Q07879-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIPYQEWHSQ LQSLYDSQIF HNWALCQDVH LNDEKDGLLL RLIPTRQLQK
60 70 80 90 100
NTERIENKLL NHIELYLTYS KVYNEPLLLL RIWEEKSIDG IPMTKLMLPT
110 120 130 140 150
DIESLLDVQG KFQLGLDTII NLEGSVWYSF HPCDTSCIVG DQAEFMSTYL
160
RRWVSIFIFS WLGYEDS
Length:167
Mass (Da):19,760
Last modified:November 1, 1996 - v1
Checksum:i9B90684B8D3D26C5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73147 Genomic DNA. Translation: CAA97493.1.
AY558190 Genomic DNA. Translation: AAS56516.1.
BK006945 Genomic DNA. Translation: DAA09280.1.
PIRiS64794.
RefSeqiNP_013058.1. NM_001181862.1.

Genome annotation databases

EnsemblFungiiYLL042C; YLL042C; YLL042C.
GeneIDi850684.
KEGGisce:YLL042C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73147 Genomic DNA. Translation: CAA97493.1 .
AY558190 Genomic DNA. Translation: AAS56516.1 .
BK006945 Genomic DNA. Translation: DAA09280.1 .
PIRi S64794.
RefSeqi NP_013058.1. NM_001181862.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4EBR X-ray 2.70 A/B 1-167 [» ]
4GSK X-ray 2.90 Y/Z 1-167 [» ]
ProteinModelPortali Q07879.
SMRi Q07879. Positions 1-165.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31271. 38 interactions.
DIPi DIP-4842N.
IntActi Q07879. 2 interactions.
MINTi MINT-487830.
STRINGi 4932.YLL042C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLL042C ; YLL042C ; YLL042C .
GeneIDi 850684.
KEGGi sce:YLL042C.

Organism-specific databases

CYGDi YLL042c.
SGDi S000003965. ATG10.

Phylogenomic databases

eggNOGi NOG70099.
InParanoidi Q07879.
KOi K08338.
OMAi RISIAIT.
OrthoDBi EOG7FBRXB.

Enzyme and pathway databases

BioCyci YEAST:G3O-32143-MONOMER.

Miscellaneous databases

NextBioi 966689.

Gene expression databases

Genevestigatori Q07879.

Family and domain databases

InterProi IPR016524. Autophagy-rel_prot_10.
IPR007135. Autophagy-rel_prot_3.
[Graphical view ]
Pfami PF03987. Autophagy_act_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF007802. Autophagy-rel_ATG10. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
    Tsukada M., Ohsumi Y.
    FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: FUNCTION.
  6. "Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast."
    Shintani T., Mizushima N., Ogawa Y., Matsuura A., Noda T., Ohsumi Y.
    EMBO J. 18:5234-5241(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG7 AND ATG12, MUTAGENESIS OF CYS-26; CYS-133 AND CYS-137.
  7. "The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
    Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
    EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: NOMENCLATURE.
  9. "Structure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy."
    Hanada T., Ohsumi Y.
    Autophagy 1:110-118(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG12.
  10. "Crystallization and preliminary X-ray analysis of Atg10."
    Yamaguti M., Suzuki N.N., Fujioka Y., Ohsumi Y., Inagaki F.
    Acta Crystallogr. F 63:443-445(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
  11. "Hierarchy of Atg proteins in pre-autophagosomal structure organization."
    Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
    Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  12. "In vivo reconstitution of autophagy in Saccharomyces cerevisiae."
    Cao Y., Cheong H., Song H., Klionsky D.J.
    J. Cell Biol. 182:703-713(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Piecemeal microautophagy of the nucleus requires the core macroautophagy genes."
    Krick R., Muehe Y., Prick T., Bremer S., Schlotterhose P., Eskelinen E.L., Millen J., Goldfarb D.S., Thumm M.
    Mol. Biol. Cell 19:4492-4505(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Purification and characterization of a ubiquitin-like system for autophagosome formation."
    Bae J.Y., Park H.H.
    J. Microbiol. Biotechnol. 20:1647-1652(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  15. "Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures."
    Kaiser S.E., Mao K., Taherbhoy A.M., Yu S., Olszewski J.L., Duda D.M., Kurinov I., Deng A., Fenn T.D., Klionsky D.J., Schulman B.A.
    Nat. Struct. Mol. Biol. 19:1242-1249(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG7.
  16. "Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8."
    Hong S.B., Kim B.W., Lee K.E., Kim S.W., Jeon H., Kim J., Song H.K.
    Nat. Struct. Mol. Biol. 18:1323-1330(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG7.
  17. "A late form of nucleophagy in Saccharomyces cerevisiae."
    Mijaljica D., Prescott M., Devenish R.J.
    PLoS ONE 7:E40013-E40013(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), INTERACTION WITH ATG7.

Entry informationi

Entry nameiATG10_YEAST
AccessioniPrimary (citable) accession number: Q07879
Secondary accession number(s): D6VXW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3