Reviewed, UniProtKB/Swiss-Prot Q07834 (YL032_YEAST)

Names and origin

Protein names

Recommended name:
    KH domain-containing protein YLL032C

Gene names

Ordered Locus Names:

YLL032C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cytoplasm Ref.2.
Miscellaneous	Present with 922 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Contains 1 KH domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 825	825	KH domain-containing protein YLL032C		PRO_0000247115
Regions
	Domain	482 – 556	75	KH
	Compositional bias	783 – 806	24	Tyr-rich
Amino acid modifications
	Modified residue	757	1	Phosphoserine Ref.5
	Modified residue	762	1	Phosphoserine Ref.5 Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q07834-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 00ADD65FBF1FA9FA	FASTA	825	94,597
	10 20 30 40 50 60 MDNFKIYSTV ITTAFLQVPH LYTTNRLWKP IEAPFLVEFL QKRISSKELK NTKAICHIDP 70 80 90 100 110 120 SWVNLNASFI RDDMISIKAT TDDMDLDAIC RISLPLPMNT NDLTAELEKM KRILLDLSEK 130 140 150 160 170 180 FNLELIITKE PAYFTPEQTG ESKELCIYVH ALGFRSNLME CEPQLLAFVD LIKKNGMTLP 190 200 210 220 230 240 PQHYIIEPME LNSYSVLPLY MGVDMENFKH ISRAFKTSIY APSLITLSRD LKANPQIFFS 250 260 270 280 290 300 GAVHSLSLLA RKTLRESISV NSKSFFYRRL TNITPGKLLF IRKYYQQKVN QLILKYQSLI 310 320 330 340 350 360 RVTNEYIEFQ SISTNLLEMV IKNFTIQVLH EIVEVQISLN ENCAMSPELI IDSFFGHTGN 370 380 390 400 410 420 QIVVITPKED SFNQLIVVGN QSSTDEASDT SILHYLSDFI MGSNQVINPN LRQIKAIFEI 430 440 450 460 470 480 HPDFEDFISG KKNGKLTRIM ELSACLIQLE MEEEDDNLYL NLVSDSFPDF KESFKNVINE 490 500 510 520 530 540 FPAEESFFIP EVCHRPIIGT GGSLIQATMR KHNVFIQFSN SFNLPQNKIS MIRYDNVIIR 550 560 570 580 590 600 CPRKNKANIC LAKNDLKQIV QEYDSLQSKT LIRFSSGQYR HILHVNGQKN IIGQIEKNEN 610 620 630 640 650 660 VYIMIPLKEP LDGTSQLSIQ GNDENASRAA NELVNSAFGY EYEFKIDQEI DPNKEYEFYN 670 680 690 700 710 720 LIVVPFLQIM NIIVTFEKDL ITFTFEKDTN ENTLTKAIEL LSNYLETQKT KIIFKKIIKK 730 740 750 760 770 780 FVLGSASSKS NTSNSNTNGN FRSMNNAKSR TTIDNTSQSG ASPQRHKMPV ITTVGGAQAI 790 800 810 820 KGYIPNTYYN GYGYGYGYTY EYDYNYANSN KAQTNNRHKY QNGRK

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-762, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-757 AND SER-762, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73137 Genomic DNA. Translation: CAA97481.1.
PIR	S64783.
RefSeq	NP_013068.1.
3D structure databases
SMR	Q07834. Positions 482-566.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1956N.
IntAct	Q07834. 2 interactions.
STRING	Q07834.
Proteomic databases
PeptideAtlas	Q07834.
Genome annotation databases
Ensembl	YLL032C; YLL032C; YLL032C; Saccharomyces cerevisiae. [Genome view]
GeneID	850627.
KEGG	sce:YLL032C.
NMPDR	fig|4932.3.peg.4059.
Organism-specific databases
CYGD	YLL032c.
SGD	S000003955. YLL032C.
Phylogenomic databases
eggNOG	fuNOG06912.
OMA	IQFSNSF.
OrthoDB	EOG9ZGQVG.
PhylomeDB	Q07834.
Gene expression databases
ArrayExpress	Q07834.
Genevestigator	Q07834.
GermOnline	YLL032C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004087. KH. IPR018111. KH_type_1_subgr. [Graphical view]
Pfam	PF00013. KH_1. 1 hit. [Graphical view]
SMART	SM00322. KH. 1 hit. [Graphical view]
PROSITE	PS50084. KH_TYPE_1. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	966538.

Entry information

Entry name

YL032_YEAST

Accession

Primary (citable) accession number: Q07834

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)