Q07825 (FRA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative Xaa-Pro aminopeptidase FRA1
EC=3.4.11.9

Alternative name(s):
Fe repressor of activation 1

Gene names

Name:	FRA1
Ordered Locus Names:	YLL029W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	749 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of the iron regulon in responss to decreased mitochondrial iron-sulfur cluster synthesis. Ref.7
Catalytic activity	Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Cofactor	Binds 2 manganese ions per subunit Potential.
Subunit structure	Homodimer By similarity. Interacts with FRA2. Ref.7
Subcellular location	Cytoplasm Ref.4.
Miscellaneous	Present with 3237 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase M24B family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription from RNA polymerase II promoter in response to iron Inferred from mutant phenotype Ref.7. Source: SGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay Ref.7. Source: SGD
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 749	749	Putative Xaa-Pro aminopeptidase FRA1		PRO_0000185086
Sites
	Metal binding	551	1	Manganese 2 Potential
	Metal binding	562	1	Manganese 1 Potential
	Metal binding	562	1	Manganese 2 Potential
	Metal binding	660	1	Manganese 1 Potential
	Metal binding	674	1	Manganese 1 Potential
	Metal binding	674	1	Manganese 2 Potential
Amino acid modifications
	Modified residue	56	1	Phosphoserine Ref.6
	Modified residue	68	1	Phosphoserine Ref.8
	Modified residue	69	1	Phosphoserine Ref.6 Ref.8
	Modified residue	95	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q07825 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 372FFF818A5FBDB9	FASTA	749	84,924
	10 20 30 40 50 60 MTSKPSTSDG RAHSISHVPG THMRGTSASH SPRPFRPCAD CTCSPGLLSR QGRRASLFLR 70 80 90 100 110 120 QLENSRRSSS MLLNELKGAG GGSSAGNGSV YSCDSLCAVN REVNTTDRLL KLRQEMKKHD 130 140 150 160 170 180 LCCYIVPSCD EHQSEYVSLR DQRRAFISGF SGSAGVACIT RDLLNFNDDH PDGKSILSTD 190 200 210 220 230 240 GRYFNQARQE LDYNWTLLRQ NEDPITWQEW CVREALEMAK GLGNKEGMVL KIGIDPKLIT 250 260 270 280 290 300 FNDYVSFRKM IDTKYDAKGK VELVPVEENL VDSIWPDFET LPERPCNDLL LLKYEFHGEE 310 320 330 340 350 360 FKDKKEKLLK KLNDKASSAT TGRNTFIVVA LDEICWLLNL RGSDIDYNPV FFSYVAINED 370 380 390 400 410 420 ETILFTNNPF NDDISEYFKI NGIEVRPYEQ IWEHLTKITS QASSAEHEFL IPDSASWQMV 430 440 450 460 470 480 RCLNTSTNAN GAIAKKMTAQ NFAIIHSPID VLKSIKNDIE IKNAHKAQVK DAVCLVQYFA 490 500 510 520 530 540 WLEQQLVGRE ALIDEYRAAE KLTEIRKTQR NFMGNSFETI SSTGSNAAII HYSPPVENSS 550 560 570 580 590 600 MIDPTKIYLC DSGSQFLEGT TDITRTIHLT KPTKEEMDNY TLVLKGGLAL ERLIFPENTP 610 620 630 640 650 660 GFNIDAIARQ FLWSRGLDYK HGTGHGIGSF LNVHEGPMGV GFRPHLMNFP LRAGNIISNE 670 680 690 700 710 720 PGYYKDGEYG IRIESDMLIK KATEKGNFLK FENMTVVPYC RKLINTKLLN EEEKTQINEY 730 740 HARVWRTIVH FLQPQSISYK WLKRETSPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	Bienvenut W.V., Peters C. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 189-199 AND 593-609, MASS SPECTROMETRY.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56 AND SER-69, MASS SPECTROMETRY.
[7]	"Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis." Kumanovics A., Chen O.S., Li L., Bagley D., Adkins E.M., Lin H., Dingra N.N., Outten C.E., Keller G., Winge D., Ward D.M., Kaplan J. J. Biol. Chem. 283:10276-10286(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH FRA2.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-69 AND SER-95, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73134 Genomic DNA. Translation: CAA97478.1. BK006945 Genomic DNA. Translation: DAA09291.1.
PIR	S64780.
RefSeq	NP_013071.1. NM_001181849.1.
3D structure databases
ProteinModelPortal	Q07825.
SMR	Q07825. Positions 348-687.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6443N.
IntAct	Q07825. 3 interactions.
MINT	MINT-677232.
STRING	4932.YLL029W.
Protein family/group databases
MEROPS	M24.A10.
Proteomic databases
PaxDb	Q07825.
PeptideAtlas	Q07825.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLL029W; YLL029W; YLL029W.
GeneID	850630.
KEGG	sce:YLL029W.
Organism-specific databases
CYGD	YLL029w.
SGD	S000003952. FRA1.
Phylogenomic databases
eggNOG	COG0006.
GeneTree	ENSGT00390000013970.
KO	K01262.
OMA	ISTARFP.
OrthoDB	EOG45F0XX.
Gene expression databases
Genevestigator	Q07825.
GermOnline	YLL029W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.90.230.10. 1 hit.
InterPro	IPR000587. Creatinase. IPR000994. Pept_M24_structural-domain. IPR001131. Peptidase_M24B_aminopep-P_CS. [Graphical view]
Pfam	PF01321. Creatinase_N. 1 hit. PF00557. Peptidase_M24. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
PROSITE	PS00491. PROLINE_PEPTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966544.

Entry information

Entry name

FRA1_YEAST

Accession

Primary (citable) accession number: Q07825
Secondary accession number(s): D6VXX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program