Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyamine transporter 1

Gene

TPO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Catalyzes polyamine uptake at alkaline pH and excretion at acidic pH. Recognizes spermidine, spermine and putrescine, the polyamine analogs methylglyoxal bis(guanylhydrazone) (MGBG) and paraquat, the antimalarial drug quinidine, and cycloheximide. Confers resistance to the non-steroidal anti-inflammatory drug indomethacin.5 Publications

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • spermidine transmembrane transporter activity Source: SGD
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

  • polyamine transmembrane transport Source: SGD
  • putrescine transport Source: SGD
  • spermidine transport Source: SGD
  • spermine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32132-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.16. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 1
Gene namesi
Name:TPO1
Ordered Locus Names:YLL028W
ORF Names:L0939
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL028W.
SGDiS000003951. TPO1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 145145CytoplasmicSequence analysisAdd
BLAST
Transmembranei146 – 16621HelicalSequence analysisAdd
BLAST
Topological domaini167 – 18115ExtracellularSequence analysisAdd
BLAST
Transmembranei182 – 20221HelicalSequence analysisAdd
BLAST
Topological domaini203 – 21210CytoplasmicSequence analysis
Transmembranei213 – 23321HelicalSequence analysisAdd
BLAST
Topological domaini234 – 2429ExtracellularSequence analysis
Transmembranei243 – 26321HelicalSequence analysisAdd
BLAST
Topological domaini264 – 2718CytoplasmicSequence analysis
Transmembranei272 – 29221HelicalSequence analysisAdd
BLAST
Topological domaini293 – 3019ExtracellularSequence analysis
Transmembranei302 – 32221HelicalSequence analysisAdd
BLAST
Topological domaini323 – 37654CytoplasmicSequence analysisAdd
BLAST
Transmembranei377 – 39721HelicalSequence analysisAdd
BLAST
Topological domaini398 – 41417ExtracellularSequence analysisAdd
BLAST
Transmembranei415 – 43521HelicalSequence analysisAdd
BLAST
Topological domaini436 – 45419CytoplasmicSequence analysisAdd
BLAST
Transmembranei455 – 47521HelicalSequence analysisAdd
BLAST
Topological domaini476 – 4838ExtracellularSequence analysis
Transmembranei484 – 50421HelicalSequence analysisAdd
BLAST
Topological domaini505 – 5051CytoplasmicSequence analysis
Transmembranei506 – 52621HelicalSequence analysisAdd
BLAST
Topological domaini527 – 5282ExtracellularSequence analysis
Transmembranei529 – 54921HelicalSequence analysisAdd
BLAST
Transmembranei550 – 57021HelicalSequence analysisAdd
BLAST
Topological domaini571 – 58616CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cellular bud membrane Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191S → A: Reduces transport activity. 1 Publication
Mutagenesisi19 – 191S → E: Enhances transport activity. 1 Publication
Mutagenesisi52 – 521T → A: Reduces transport activity. 1 Publication
Mutagenesisi52 – 521T → E: Enhances transport activity. 1 Publication
Mutagenesisi207 – 2071E → Q: Loss of function. 1 Publication
Mutagenesisi323 – 3231E → Q: Loss of function; when associated with Q-324. 1 Publication
Mutagenesisi324 – 3241E → Q: Loss of function; when associated with Q-323. 1 Publication
Mutagenesisi342 – 3421S → A: Interferes with correct plasma membrane localization. 1 Publication
Mutagenesisi342 – 3421S → E: Enhances transport activity. 1 Publication
Mutagenesisi574 – 5741E → Q: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586Polyamine transporter 1PRO_0000262730Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine; by PKC1 Publication
Modified residuei52 – 521Phosphothreonine; by CK11 Publication
Modified residuei72 – 721PhosphoserineCombined sources
Modified residuei76 – 761PhosphoserineCombined sources
Modified residuei89 – 891PhosphothreonineCombined sources
Modified residuei91 – 911PhosphoserineCombined sources
Modified residuei342 – 3421Phosphoserine; by PKA1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ07824.

PTM databases

iPTMnetiQ07824.

Expressioni

Inductioni

By transcription factor PDR1, by transcription factor HAA1 in response to acetaldehyde accumulation and by the non-steroidal anti-inflammatory drug indomethacin.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi31224. 80 interactions.
DIPiDIP-1706N.
IntActiQ07824. 29 interactions.
MINTiMINT-384315.

Structurei

3D structure databases

ProteinModelPortaliQ07824.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 259Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ07824.
KOiK08157.
OMAiKEVHWIV.
OrthoDBiEOG092C2079.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDHSPISNK ENHLLPSDSS RSSSSDMHST GTTGTTGVEP VDFTGEGAKY
60 70 80 90 100
TTATEGNGGA DLAIQRTTTM NSAAESEVNI TRRLTKILTG SVNEPDRVEV
110 120 130 140 150
DYTNCAPMGG DRPYPPSLPS RDLYEVTFDG PNDPLHPFNW PMKKKVLLCL
160 170 180 190 200
VLCLDSIAIA MCSSIFASAV PQICEIYHVI EVVAILGITL FVLGFAASPV
210 220 230 240 250
IYAPLSELYG RKGVLVLSAF GFALFQFAVA TAENLQTIFI CRFFGGFIGA
260 270 280 290 300
APMAVVPAAF ADMFDTNVRG KAIALFSLGV FVGPILSPVM GSYIAQRTTW
310 320 330 340 350
RWLEYVVGCF ASAVFVAIVL FFEETHHPTI LVNKAKQMRK QSNNWGIHAA
360 370 380 390 400
HEDVELSIKD IVQKTVTRPI IMLFVEPLLL FVTIYNSFVY GILYLLLEAY
410 420 430 440 450
PLVFVEGYGF TENGELPYIA LIIGMMVCAA FIWYMDNDYL KRCRAKGKLV
460 470 480 490 500
PEARLYAMVI AGTVFPIGIL WFCWTGYYPH KIHWMVPTVG GAFIGFGLMG
510 520 530 540 550
IFLPCLNYII ESYLLLAASA VAANTFMRSA FGACFPLFAG YMFRGMGIGW
560 570 580
AGLLLGLFAA AMIPVPLLFL KYGESIRKKS KYAYAA
Length:586
Mass (Da):64,272
Last modified:November 1, 1996 - v1
Checksum:i32DBFB795617D036
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73133 Genomic DNA. Translation: CAA97477.1.
BK006945 Genomic DNA. Translation: DAA09292.1.
PIRiS64779.
RefSeqiNP_013072.1. NM_001181848.1.

Genome annotation databases

EnsemblFungiiYLL028W; YLL028W; YLL028W.
GeneIDi850631.
KEGGisce:YLL028W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73133 Genomic DNA. Translation: CAA97477.1.
BK006945 Genomic DNA. Translation: DAA09292.1.
PIRiS64779.
RefSeqiNP_013072.1. NM_001181848.1.

3D structure databases

ProteinModelPortaliQ07824.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31224. 80 interactions.
DIPiDIP-1706N.
IntActiQ07824. 29 interactions.
MINTiMINT-384315.

Protein family/group databases

TCDBi2.A.1.2.16. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ07824.

Proteomic databases

MaxQBiQ07824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL028W; YLL028W; YLL028W.
GeneIDi850631.
KEGGisce:YLL028W.

Organism-specific databases

EuPathDBiFungiDB:YLL028W.
SGDiS000003951. TPO1.

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ07824.
KOiK08157.
OMAiKEVHWIV.
OrthoDBiEOG092C2079.

Enzyme and pathway databases

BioCyciYEAST:G3O-32132-MONOMER.

Miscellaneous databases

PROiQ07824.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPO1_YEAST
AccessioniPrimary (citable) accession number: Q07824
Secondary accession number(s): D6VXX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.