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Protein

Polyamine transporter 1

Gene

TPO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Catalyzes polyamine uptake at alkaline pH and excretion at acidic pH. Recognizes spermidine, spermine and putrescine, the polyamine analogs methylglyoxal bis(guanylhydrazone) (MGBG) and paraquat, the antimalarial drug quinidine, and cycloheximide. Confers resistance to the non-steroidal anti-inflammatory drug indomethacin.5 Publications

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • spermidine transmembrane transporter activity Source: SGD
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

  • polyamine transmembrane transport Source: SGD
  • putrescine transport Source: SGD
  • spermidine transport Source: SGD
  • spermine transport Source: SGD

Keywordsi

Biological processAntiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32132-MONOMER

Protein family/group databases

TCDBi2.A.1.2.16 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 1
Gene namesi
Name:TPO1
Ordered Locus Names:YLL028W
ORF Names:L0939
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL028W
SGDiS000003951 TPO1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 145CytoplasmicSequence analysisAdd BLAST145
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167 – 181ExtracellularSequence analysisAdd BLAST15
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Topological domaini203 – 212CytoplasmicSequence analysis10
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 242ExtracellularSequence analysis9
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 271CytoplasmicSequence analysis8
Transmembranei272 – 292HelicalSequence analysisAdd BLAST21
Topological domaini293 – 301ExtracellularSequence analysis9
Transmembranei302 – 322HelicalSequence analysisAdd BLAST21
Topological domaini323 – 376CytoplasmicSequence analysisAdd BLAST54
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Topological domaini398 – 414ExtracellularSequence analysisAdd BLAST17
Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
Topological domaini436 – 454CytoplasmicSequence analysisAdd BLAST19
Transmembranei455 – 475HelicalSequence analysisAdd BLAST21
Topological domaini476 – 483ExtracellularSequence analysis8
Transmembranei484 – 504HelicalSequence analysisAdd BLAST21
Topological domaini505CytoplasmicSequence analysis1
Transmembranei506 – 526HelicalSequence analysisAdd BLAST21
Topological domaini527 – 528ExtracellularSequence analysis2
Transmembranei529 – 549HelicalSequence analysisAdd BLAST21
Transmembranei550 – 570HelicalSequence analysisAdd BLAST21
Topological domaini571 – 586CytoplasmicSequence analysisAdd BLAST16

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19S → A: Reduces transport activity. 1 Publication1
Mutagenesisi19S → E: Enhances transport activity. 1 Publication1
Mutagenesisi52T → A: Reduces transport activity. 1 Publication1
Mutagenesisi52T → E: Enhances transport activity. 1 Publication1
Mutagenesisi207E → Q: Loss of function. 1 Publication1
Mutagenesisi323E → Q: Loss of function; when associated with Q-324. 1 Publication1
Mutagenesisi324E → Q: Loss of function; when associated with Q-323. 1 Publication1
Mutagenesisi342S → A: Interferes with correct plasma membrane localization. 1 Publication1
Mutagenesisi342S → E: Enhances transport activity. 1 Publication1
Mutagenesisi574E → Q: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002627301 – 586Polyamine transporter 1Add BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Phosphoserine; by PKC1 Publication1
Modified residuei52Phosphothreonine; by CK11 Publication1
Modified residuei72PhosphoserineCombined sources1
Modified residuei76PhosphoserineCombined sources1
Modified residuei89PhosphothreonineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei342Phosphoserine; by PKA1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ07824
PaxDbiQ07824
PRIDEiQ07824

PTM databases

iPTMnetiQ07824

Expressioni

Inductioni

By transcription factor PDR1, by transcription factor HAA1 in response to acetaldehyde accumulation and by the non-steroidal anti-inflammatory drug indomethacin.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi31224, 137 interactors
DIPiDIP-1706N
IntActiQ07824, 29 interactors
MINTiQ07824
STRINGi4932.YLL028W

Structurei

3D structure databases

ProteinModelPortaliQ07824
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi17 – 25Poly-Ser9

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088227
HOGENOMiHOG000160689
InParanoidiQ07824
KOiK08157
OMAiGACPLAV
OrthoDBiEOG092C2079

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequencei

Sequence statusi: Complete.

Q07824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDHSPISNK ENHLLPSDSS RSSSSDMHST GTTGTTGVEP VDFTGEGAKY
60 70 80 90 100
TTATEGNGGA DLAIQRTTTM NSAAESEVNI TRRLTKILTG SVNEPDRVEV
110 120 130 140 150
DYTNCAPMGG DRPYPPSLPS RDLYEVTFDG PNDPLHPFNW PMKKKVLLCL
160 170 180 190 200
VLCLDSIAIA MCSSIFASAV PQICEIYHVI EVVAILGITL FVLGFAASPV
210 220 230 240 250
IYAPLSELYG RKGVLVLSAF GFALFQFAVA TAENLQTIFI CRFFGGFIGA
260 270 280 290 300
APMAVVPAAF ADMFDTNVRG KAIALFSLGV FVGPILSPVM GSYIAQRTTW
310 320 330 340 350
RWLEYVVGCF ASAVFVAIVL FFEETHHPTI LVNKAKQMRK QSNNWGIHAA
360 370 380 390 400
HEDVELSIKD IVQKTVTRPI IMLFVEPLLL FVTIYNSFVY GILYLLLEAY
410 420 430 440 450
PLVFVEGYGF TENGELPYIA LIIGMMVCAA FIWYMDNDYL KRCRAKGKLV
460 470 480 490 500
PEARLYAMVI AGTVFPIGIL WFCWTGYYPH KIHWMVPTVG GAFIGFGLMG
510 520 530 540 550
IFLPCLNYII ESYLLLAASA VAANTFMRSA FGACFPLFAG YMFRGMGIGW
560 570 580
AGLLLGLFAA AMIPVPLLFL KYGESIRKKS KYAYAA
Length:586
Mass (Da):64,272
Last modified:November 1, 1996 - v1
Checksum:i32DBFB795617D036
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73133 Genomic DNA Translation: CAA97477.1
BK006945 Genomic DNA Translation: DAA09292.1
PIRiS64779
RefSeqiNP_013072.1, NM_001181848.1

Genome annotation databases

EnsemblFungiiYLL028W; YLL028W; YLL028W
GeneIDi850631
KEGGisce:YLL028W

Similar proteinsi

Entry informationi

Entry nameiTPO1_YEAST
AccessioniPrimary (citable) accession number: Q07824
Secondary accession number(s): D6VXX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health