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Protein

Polyamine transporter 1

Gene

TPO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Catalyzes polyamine uptake at alkaline pH and excretion at acidic pH. Recognizes spermidine, spermine and putrescine, the polyamine analogs methylglyoxal bis(guanylhydrazone) (MGBG) and paraquat, the antimalarial drug quinidine, and cycloheximide. Confers resistance to the non-steroidal anti-inflammatory drug indomethacin.5 Publications

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • spermidine transmembrane transporter activity Source: SGD
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

  • polyamine transmembrane transport Source: SGD
  • putrescine transport Source: SGD
  • spermidine transport Source: SGD
  • spermine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32132-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.16. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 1
Gene namesi
Name:TPO1
Ordered Locus Names:YLL028W
ORF Names:L0939
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL028W.
SGDiS000003951. TPO1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 145CytoplasmicSequence analysisAdd BLAST145
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167 – 181ExtracellularSequence analysisAdd BLAST15
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Topological domaini203 – 212CytoplasmicSequence analysis10
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 242ExtracellularSequence analysis9
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 271CytoplasmicSequence analysis8
Transmembranei272 – 292HelicalSequence analysisAdd BLAST21
Topological domaini293 – 301ExtracellularSequence analysis9
Transmembranei302 – 322HelicalSequence analysisAdd BLAST21
Topological domaini323 – 376CytoplasmicSequence analysisAdd BLAST54
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Topological domaini398 – 414ExtracellularSequence analysisAdd BLAST17
Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
Topological domaini436 – 454CytoplasmicSequence analysisAdd BLAST19
Transmembranei455 – 475HelicalSequence analysisAdd BLAST21
Topological domaini476 – 483ExtracellularSequence analysis8
Transmembranei484 – 504HelicalSequence analysisAdd BLAST21
Topological domaini505CytoplasmicSequence analysis1
Transmembranei506 – 526HelicalSequence analysisAdd BLAST21
Topological domaini527 – 528ExtracellularSequence analysis2
Transmembranei529 – 549HelicalSequence analysisAdd BLAST21
Transmembranei550 – 570HelicalSequence analysisAdd BLAST21
Topological domaini571 – 586CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

  • cellular bud membrane Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19S → A: Reduces transport activity. 1 Publication1
Mutagenesisi19S → E: Enhances transport activity. 1 Publication1
Mutagenesisi52T → A: Reduces transport activity. 1 Publication1
Mutagenesisi52T → E: Enhances transport activity. 1 Publication1
Mutagenesisi207E → Q: Loss of function. 1 Publication1
Mutagenesisi323E → Q: Loss of function; when associated with Q-324. 1 Publication1
Mutagenesisi324E → Q: Loss of function; when associated with Q-323. 1 Publication1
Mutagenesisi342S → A: Interferes with correct plasma membrane localization. 1 Publication1
Mutagenesisi342S → E: Enhances transport activity. 1 Publication1
Mutagenesisi574E → Q: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002627301 – 586Polyamine transporter 1Add BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Phosphoserine; by PKC1 Publication1
Modified residuei52Phosphothreonine; by CK11 Publication1
Modified residuei72PhosphoserineCombined sources1
Modified residuei76PhosphoserineCombined sources1
Modified residuei89PhosphothreonineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei342Phosphoserine; by PKA1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ07824.
PRIDEiQ07824.

PTM databases

iPTMnetiQ07824.

Expressioni

Inductioni

By transcription factor PDR1, by transcription factor HAA1 in response to acetaldehyde accumulation and by the non-steroidal anti-inflammatory drug indomethacin.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi31224. 80 interactors.
DIPiDIP-1706N.
IntActiQ07824. 29 interactors.
MINTiMINT-384315.

Structurei

3D structure databases

ProteinModelPortaliQ07824.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi17 – 25Poly-Ser9

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ07824.
KOiK08157.
OMAiKEVHWIV.
OrthoDBiEOG092C2079.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDHSPISNK ENHLLPSDSS RSSSSDMHST GTTGTTGVEP VDFTGEGAKY
60 70 80 90 100
TTATEGNGGA DLAIQRTTTM NSAAESEVNI TRRLTKILTG SVNEPDRVEV
110 120 130 140 150
DYTNCAPMGG DRPYPPSLPS RDLYEVTFDG PNDPLHPFNW PMKKKVLLCL
160 170 180 190 200
VLCLDSIAIA MCSSIFASAV PQICEIYHVI EVVAILGITL FVLGFAASPV
210 220 230 240 250
IYAPLSELYG RKGVLVLSAF GFALFQFAVA TAENLQTIFI CRFFGGFIGA
260 270 280 290 300
APMAVVPAAF ADMFDTNVRG KAIALFSLGV FVGPILSPVM GSYIAQRTTW
310 320 330 340 350
RWLEYVVGCF ASAVFVAIVL FFEETHHPTI LVNKAKQMRK QSNNWGIHAA
360 370 380 390 400
HEDVELSIKD IVQKTVTRPI IMLFVEPLLL FVTIYNSFVY GILYLLLEAY
410 420 430 440 450
PLVFVEGYGF TENGELPYIA LIIGMMVCAA FIWYMDNDYL KRCRAKGKLV
460 470 480 490 500
PEARLYAMVI AGTVFPIGIL WFCWTGYYPH KIHWMVPTVG GAFIGFGLMG
510 520 530 540 550
IFLPCLNYII ESYLLLAASA VAANTFMRSA FGACFPLFAG YMFRGMGIGW
560 570 580
AGLLLGLFAA AMIPVPLLFL KYGESIRKKS KYAYAA
Length:586
Mass (Da):64,272
Last modified:November 1, 1996 - v1
Checksum:i32DBFB795617D036
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73133 Genomic DNA. Translation: CAA97477.1.
BK006945 Genomic DNA. Translation: DAA09292.1.
PIRiS64779.
RefSeqiNP_013072.1. NM_001181848.1.

Genome annotation databases

EnsemblFungiiYLL028W; YLL028W; YLL028W.
GeneIDi850631.
KEGGisce:YLL028W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73133 Genomic DNA. Translation: CAA97477.1.
BK006945 Genomic DNA. Translation: DAA09292.1.
PIRiS64779.
RefSeqiNP_013072.1. NM_001181848.1.

3D structure databases

ProteinModelPortaliQ07824.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31224. 80 interactors.
DIPiDIP-1706N.
IntActiQ07824. 29 interactors.
MINTiMINT-384315.

Protein family/group databases

TCDBi2.A.1.2.16. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ07824.

Proteomic databases

MaxQBiQ07824.
PRIDEiQ07824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL028W; YLL028W; YLL028W.
GeneIDi850631.
KEGGisce:YLL028W.

Organism-specific databases

EuPathDBiFungiDB:YLL028W.
SGDiS000003951. TPO1.

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ07824.
KOiK08157.
OMAiKEVHWIV.
OrthoDBiEOG092C2079.

Enzyme and pathway databases

BioCyciYEAST:G3O-32132-MONOMER.

Miscellaneous databases

PROiQ07824.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPO1_YEAST
AccessioniPrimary (citable) accession number: Q07824
Secondary accession number(s): D6VXX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.