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Protein

Iron-sulfur assembly protein 1

Gene

ISA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi178 – 1781IronBy similarity
Metal bindingi242 – 2421IronBy similarity
Metal bindingi244 – 2441IronBy similarity

GO - Molecular functioni

  • iron ion binding Source: SGD
  • iron-sulfur cluster binding Source: SGD
  • structural molecule activity Source: InterPro

GO - Biological processi

  • biotin biosynthetic process Source: SGD
  • iron-sulfur cluster assembly Source: SGD
Complete GO annotation...

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32131-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur assembly protein 1
Gene namesi
Name:ISA1
Ordered Locus Names:YLL027W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL027W.
SGDiS000003950. ISA1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250Iron-sulfur assembly protein 1PRO_0000077034Add
BLAST

Proteomic databases

MaxQBiQ07821.
PeptideAtlasiQ07821.

Interactioni

Protein-protein interaction databases

BioGridi31225. 23 interactions.
DIPiDIP-1394N.
IntActiQ07821. 4 interactions.
MINTiMINT-396836.

Structurei

3D structure databases

ProteinModelPortaliQ07821.
SMRiQ07821. Positions 147-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HesB/IscA family.Curated

Phylogenomic databases

GeneTreeiENSGT00490000043385.
HOGENOMiHOG000216851.
InParanoidiQ07821.
KOiK13628.
OMAiTIKSHEE.
OrthoDBiEOG7DFXRB.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR016092. FeS_cluster_insertion.
IPR017870. FeS_cluster_insertion_CS.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR00049. TIGR00049. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINTGRSRNS VLLAHRFLST GGFWRGGTNG TMSRTINNVN PFKLKFIPKT
60 70 80 90 100
VPAAADSVSP DSQRPGKKPF KFIVSNQSKS SKASKSPKWS SYAFPSRETI
110 120 130 140 150
KSHEEAIKKQ NKAIDEQIAA AVSKNDCSCT EPPKKRKRKL RPRKALITLS
160 170 180 190 200
PKAIKHLRAL LAQPEPKLIR VSARNRGCSG LTYDLQYITE PGKFDEVVEQ
210 220 230 240 250
DGVKIVIDSK ALFSIIGSEM DWIDDKLASK FVFKNPNSKG TCGCGESFMV
Length:250
Mass (Da):27,675
Last modified:November 1, 1996 - v1
Checksum:i226C286B4F80DE6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73132 Genomic DNA. Translation: CAA97476.1.
BK006945 Genomic DNA. Translation: DAA09293.1.
PIRiS64778.
RefSeqiNP_013073.1. NM_001181847.1.

Genome annotation databases

EnsemblFungiiYLL027W; YLL027W; YLL027W.
GeneIDi850632.
KEGGisce:YLL027W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73132 Genomic DNA. Translation: CAA97476.1.
BK006945 Genomic DNA. Translation: DAA09293.1.
PIRiS64778.
RefSeqiNP_013073.1. NM_001181847.1.

3D structure databases

ProteinModelPortaliQ07821.
SMRiQ07821. Positions 147-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31225. 23 interactions.
DIPiDIP-1394N.
IntActiQ07821. 4 interactions.
MINTiMINT-396836.

Proteomic databases

MaxQBiQ07821.
PeptideAtlasiQ07821.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL027W; YLL027W; YLL027W.
GeneIDi850632.
KEGGisce:YLL027W.

Organism-specific databases

EuPathDBiFungiDB:YLL027W.
SGDiS000003950. ISA1.

Phylogenomic databases

GeneTreeiENSGT00490000043385.
HOGENOMiHOG000216851.
InParanoidiQ07821.
KOiK13628.
OMAiTIKSHEE.
OrthoDBiEOG7DFXRB.

Enzyme and pathway databases

BioCyciYEAST:G3O-32131-MONOMER.

Miscellaneous databases

NextBioi966550.
PROiQ07821.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR016092. FeS_cluster_insertion.
IPR017870. FeS_cluster_insertion_CS.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
TIGRFAMsiTIGR00049. TIGR00049. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Isa1p is a component of the mitochondrial machinery for maturation of cellular iron-sulfur proteins and requires conserved cysteine residues for function."
    Kaut A., Lange H., Diekert K., Kispal G., Lill R.
    J. Biol. Chem. 275:15955-15961(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiISA1_YEAST
AccessioniPrimary (citable) accession number: Q07821
Secondary accession number(s): D6VXX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.