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Protein

Iron-sulfur assembly protein 1

Gene

ISA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.1 Publication

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi178IronBy similarity1
Metal bindingi242IronBy similarity1
Metal bindingi244IronBy similarity1

GO - Molecular functioni

GO - Biological processi

  • biotin biosynthetic process Source: SGD
  • iron-sulfur cluster assembly Source: SGD
  • protein maturation by iron-sulfur cluster transfer Source: SGD

Keywordsi

LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32131-MONOMER
ReactomeiR-SCE-1362409 Mitochondrial iron-sulfur cluster biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur assembly protein 1
Gene namesi
Name:ISA1
Ordered Locus Names:YLL027W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL027W
SGDiS000003950 ISA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000770341 – 250Iron-sulfur assembly protein 1Add BLAST250

Proteomic databases

MaxQBiQ07821
PaxDbiQ07821
PRIDEiQ07821

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CALCOCO2Q131373EBI-27136,EBI-739580From Homo sapiens.

Protein-protein interaction databases

BioGridi3122578 interactors.
DIPiDIP-1394N
IntActiQ07821 17 interactors.
MINTiQ07821
STRINGi4932.YLL027W

Structurei

3D structure databases

ProteinModelPortaliQ07821
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HesB/IscA family.Curated

Phylogenomic databases

GeneTreeiENSGT00490000043385
HOGENOMiHOG000216851
InParanoidiQ07821
KOiK22063
OMAiSPRAMTH
OrthoDBiEOG092C5612

Family and domain databases

Gene3Di2.60.300.121 hit
InterProiView protein in InterPro
IPR000361 FeS_biogenesis
IPR016092 FeS_cluster_insertion
IPR017870 FeS_cluster_insertion_CS
IPR035903 HesB-like_dom_sf
PfamiView protein in Pfam
PF01521 Fe-S_biosyn, 1 hit
SUPFAMiSSF89360 SSF89360, 1 hit
TIGRFAMsiTIGR00049 TIGR00049, 1 hit
PROSITEiView protein in PROSITE
PS01152 HESB, 1 hit

Sequencei

Sequence statusi: Complete.

Q07821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINTGRSRNS VLLAHRFLST GGFWRGGTNG TMSRTINNVN PFKLKFIPKT
60 70 80 90 100
VPAAADSVSP DSQRPGKKPF KFIVSNQSKS SKASKSPKWS SYAFPSRETI
110 120 130 140 150
KSHEEAIKKQ NKAIDEQIAA AVSKNDCSCT EPPKKRKRKL RPRKALITLS
160 170 180 190 200
PKAIKHLRAL LAQPEPKLIR VSARNRGCSG LTYDLQYITE PGKFDEVVEQ
210 220 230 240 250
DGVKIVIDSK ALFSIIGSEM DWIDDKLASK FVFKNPNSKG TCGCGESFMV
Length:250
Mass (Da):27,675
Last modified:November 1, 1996 - v1
Checksum:i226C286B4F80DE6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73132 Genomic DNA Translation: CAA97476.1
BK006945 Genomic DNA Translation: DAA09293.1
PIRiS64778
RefSeqiNP_013073.1, NM_001181847.1

Genome annotation databases

EnsemblFungiiYLL027W; YLL027W; YLL027W
GeneIDi850632
KEGGisce:YLL027W

Similar proteinsi

Entry informationi

Entry nameiISA1_YEAST
AccessioniPrimary (citable) accession number: Q07821
Secondary accession number(s): D6VXX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome