Q07816 (B2CL1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bcl-2-like protein 1
Short name=Bcl2-L-1

Alternative name(s):
Apoptosis regulator Bcl-X

Gene names

Name:	BCL2L1
Synonyms:	BCL-X, BCLX

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dominant regulator of apoptotic cell death. The long form displays cell death repressor activity, whereas the short isoform promotes apoptosis. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis By similarity.
Subcellular location	Mitochondrion membrane; Single-pass membrane protein By similarity. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Note: Mitochondrial membranes and perinuclear envelope By similarity.
Tissue specificity	Highest expression in organs with lymphoid development.
Domain	The BH4 motif seems to be involved in the anti-apoptotic function. Intact BH1 and BH2 motifs are required for anti-apoptotic activity By similarity.
Sequence similarities	Belongs to the Bcl-2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Cytoskeleton Membrane Mitochondrion Nucleus
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Inferred from electronic annotation. Source: Compara cellular process regulating host cell cycle in response to virus Inferred from electronic annotation. Source: Compara cellular response to alkaloid Inferred from electronic annotation. Source: Compara cellular response to amino acid stimulus Inferred from electronic annotation. Source: Compara cellular response to gamma radiation Inferred from electronic annotation. Source: Compara cytokinesis Inferred from sequence or structural similarity. Source: UniProtKB fertilization Inferred from electronic annotation. Source: Compara germ cell development Inferred from electronic annotation. Source: Compara growth Inferred from electronic annotation. Source: Compara in utero embryonic development Inferred from electronic annotation. Source: Compara male gonad development Inferred from electronic annotation. Source: Compara mitotic cell cycle checkpoint Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of anoikis Inferred from electronic annotation. Source: Compara negative regulation of establishment of protein localization to plasma membrane Inferred from electronic annotation. Source: Compara negative regulation of intrinsic apoptotic signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara ovarian follicle development Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara regulation of mitochondrial membrane permeability Inferred from electronic annotation. Source: Compara regulation of mitochondrial membrane potential Inferred from electronic annotation. Source: Compara release of cytochrome c from mitochondria Inferred from electronic annotation. Source: Compara response to cycloheximide Inferred from electronic annotation. Source: Compara response to cytokine stimulus Inferred from electronic annotation. Source: Compara spermatogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	Bcl-2 family protein complex Inferred from electronic annotation. Source: Compara centrosome Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q07816-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q07816-2)

The sequence of this isoform differs from the canonical sequence as follows:
     185-229: ERFVDLYGNNAAAELRKGQETFNKWLLTGATVAGVLLLGSLLSRK → VRTALP

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Bcl-2-like protein 1		PRO_0000143061
Regions
	Transmembrane	206 – 223	18	Helical; Potential
	Motif	4 – 24	21	BH4
	Motif	82 – 96	15	BH3
	Motif	125 – 144	20	BH1
	Motif	176 – 191	16	BH2
Natural variations
	Alternative sequence	185 – 229	45	ERFVD…LLSRK → VRTALP in isoform Short.		VSP_000514

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1997. Version 2. Checksum: A97D3A4D04C0E9DA	FASTA	229	25,733
	10 20 30 40 50 60 MSSSNRELVI DFVSYKLSQR GHCWSELEEE DENRTDTAAE AEMDSVLNGS PSWHPPAGHV 70 80 90 100 110 120 VNGATVHRSS LEVHEIVRAS DVRQALRDAG DEFELRYRRA FSDLTSQLHI TPGTAYQSFE 130 140 150 160 170 180 QVVNELFHDG VNWGRIVAFF SFGGALCVES VDKEMRVLVG RIVSWMTTYL TDHLDPWIQE 190 200 210 220 NGGWERFVDL YGNNAAAELR KGQETFNKWL LTGATVAGVL LLGSLLSRK
	Isoform Short [UniParc]. Checksum: 88F5AC2C5ABDF1AA	FASTA	190	21,467
	10 20 30 40 50 60 MSSSNRELVI DFVSYKLSQR GHCWSELEEE DENRTDTAAE AEMDSVLNGS PSWHPPAGHV 70 80 90 100 110 120 VNGATVHRSS LEVHEIVRAS DVRQALRDAG DEFELRYRRA FSDLTSQLHI TPGTAYQSFE 130 140 150 160 170 180 QVVNELFHDG VNWGRIVAFF SFGGALCVES VDKEMRVLVG RIVSWMTTYL TDHLDPWIQE 190 NGGWVRTALP

References

[1]	"bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death." Boise L.H., Gonzalez-Garcia M., Postema C.E., Ding L., Lindsten T., Turka L.A., Mao X., Nunez G., Thompson C.B. Cell 74:597-608(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
[2]	"Differential expression of bcl-2 and bcl-x during chicken spermatogenesis." Vilagrasa X., Mezquita C., Mezquita J. Mol. Reprod. Dev. 47:26-29(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG). Strain: Hubbard White Mountain. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z23110 Genomic DNA. Translation: CAA80657.1. U26645 mRNA. Translation: AAB07677.1.
IPI	IPI00576590. IPI00594932.
PIR	A47537.
RefSeq	NP_001020475.1. NM_001025304.1.
UniGene	Gga.4944.
3D structure databases
ProteinModelPortal	Q07816.
SMR	Q07816. Positions 1-204.
ModBase	Search...
Proteomic databases
PaxDb	Q07816.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000010073; ENSGALP00000010059; ENSGALG00000006211.
GeneID	373954.
KEGG	gga:373954.
Organism-specific databases
CTD	598.
Phylogenomic databases
eggNOG	NOG300479.
GeneTree	ENSGT00530000062935.
HOGENOM	HOG000056452.
InParanoid	Q07816.
KO	K04570.
OMA	NGSPSWH.
Gene expression databases
ArrayExpress	Q07816.
Family and domain databases
InterPro	IPR013279. Apop_reg_BclX. IPR002475. Bcl2-like. IPR004725. Bcl2/BclX. IPR020717. Bcl2_BH1_motif_CS. IPR020726. Bcl2_BH2_motif_CS. IPR020728. Bcl2_BH3_motif_CS. IPR003093. Bcl2_BH4. IPR020731. Bcl2_BH4_motif_CS. IPR026298. Blc2_fam. [Graphical view]
PANTHER	PTHR11256. PTHR11256. 1 hit. PTHR11256:SF12. PTHR11256:SF12. 1 hit.
Pfam	PF00452. Bcl-2. 1 hit. PF02180. BH4. 1 hit. [Graphical view]
PRINTS	PR01864. APOPREGBCLX. PR01862. BCL2FAMILY.
TIGRFAMs	TIGR00865. bcl-2. 1 hit.
PROSITE	PS50062. BCL2_FAMILY. 1 hit. PS01080. BH1. 1 hit. PS01258. BH2. 1 hit. PS01259. BH3. 1 hit. PS01260. BH4_1. 1 hit. PS50063. BH4_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20813485.

Entry information

Entry name

B2CL1_CHICK

Accession

Primary (citable) accession number: Q07816
Secondary accession number(s): Q98908

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program