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Q07813

- BAX_MOUSE

UniProt

Q07813 - BAX_MOUSE

Protein

Apoptosis regulator BAX

Gene

Bax

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 151 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production.2 Publications

    GO - Molecular functioni

    1. channel activity Source: UniProtKB
    2. lipid binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein heterodimerization activity Source: MGI
    5. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    2. activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: MGI
    3. activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: MGI
    4. apoptotic mitochondrial changes Source: MGI
    5. apoptotic process Source: MGI
    6. apoptotic process involved in embryonic digit morphogenesis Source: MGI
    7. apoptotic process involved in patterning of blood vessels Source: MGI
    8. apoptotic signaling pathway Source: MGI
    9. B cell apoptotic process Source: MGI
    10. B cell homeostasis Source: MGI
    11. B cell homeostatic proliferation Source: MGI
    12. B cell negative selection Source: MGI
    13. B cell receptor apoptotic signaling pathway Source: Ensembl
    14. blood vessel remodeling Source: MGI
    15. cell proliferation Source: MGI
    16. cellular response to DNA damage stimulus Source: MGI
    17. cellular response to organic substance Source: MGI
    18. cellular response to UV Source: MGI
    19. cerebral cortex development Source: MGI
    20. development of secondary sexual characteristics Source: MGI
    21. ectopic germ cell programmed cell death Source: MGI
    22. establishment or maintenance of transmembrane electrochemical gradient Source: UniProtKB
    23. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
    24. extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
    25. fertilization Source: MGI
    26. germ cell development Source: MGI
    27. glycosphingolipid metabolic process Source: MGI
    28. homeostasis of number of cells Source: MGI
    29. homeostasis of number of cells within a tissue Source: MGI
    30. hypothalamus development Source: MGI
    31. intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
    32. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
    33. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
    34. kidney development Source: MGI
    35. leukocyte homeostasis Source: MGI
    36. limb morphogenesis Source: MGI
    37. male gonad development Source: MGI
    38. mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
    39. mitochondrial fusion Source: UniProtKB
    40. mitochondrion morphogenesis Source: MGI
    41. myeloid cell homeostasis Source: MGI
    42. negative regulation of apoptotic signaling pathway Source: MGI
    43. negative regulation of cell proliferation Source: MGI
    44. negative regulation of endoplasmic reticulum calcium ion concentration Source: MGI
    45. negative regulation of fibroblast proliferation Source: MGI
    46. negative regulation of neuron apoptotic process Source: MGI
    47. negative regulation of peptidyl-serine phosphorylation Source: MGI
    48. negative regulation of protein binding Source: UniProtKB
    49. nervous system development Source: MGI
    50. neuron apoptotic process Source: MGI
    51. neuron migration Source: MGI
    52. odontogenesis of dentin-containing tooth Source: MGI
    53. ovarian follicle development Source: MGI
    54. positive regulation of apoptotic DNA fragmentation Source: Ensembl
    55. positive regulation of apoptotic process Source: MGI
    56. positive regulation of apoptotic process involved in mammary gland involution Source: MGI
    57. positive regulation of B cell apoptotic process Source: MGI
    58. positive regulation of calcium ion transport into cytosol Source: MGI
    59. positive regulation of developmental pigmentation Source: MGI
    60. positive regulation of endoplasmic reticulum unfolded protein response Source: UniProtKB
    61. positive regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
    62. positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
    63. positive regulation of mitochondrial membrane permeability involved in apoptotic process Source: MGI
    64. positive regulation of neuron apoptotic process Source: MGI
    65. positive regulation of protein oligomerization Source: UniProtKB
    66. positive regulation of release of cytochrome c from mitochondria Source: UniProtKB
    67. positive regulation of release of sequestered calcium ion into cytosol Source: MGI
    68. post-embryonic camera-type eye morphogenesis Source: MGI
    69. post-embryonic development Source: MGI
    70. protein homooligomerization Source: MGI
    71. protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: MGI
    72. regulation of cell cycle Source: MGI
    73. regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
    74. regulation of mammary gland epithelial cell proliferation Source: MGI
    75. regulation of mitochondrial membrane permeability involved in apoptotic process Source: MGI
    76. regulation of mitochondrial membrane permeability involved in programmed necrotic cell death Source: MGI
    77. regulation of neuron apoptotic process Source: MGI
    78. regulation of nitrogen utilization Source: MGI
    79. regulation of protein heterodimerization activity Source: Ensembl
    80. regulation of protein homodimerization activity Source: UniProtKB
    81. release of cytochrome c from mitochondria Source: MGI
    82. release of matrix enzymes from mitochondria Source: UniProtKB
    83. response to axon injury Source: MGI
    84. response to gamma radiation Source: MGI
    85. response to ionizing radiation Source: MGI
    86. response to salt stress Source: MGI
    87. response to toxic substance Source: MGI
    88. response to wounding Source: MGI
    89. retina development in camera-type eye Source: MGI
    90. retinal cell apoptotic process Source: UniProtKB
    91. retinal cell programmed cell death Source: MGI
    92. Sertoli cell proliferation Source: MGI
    93. sex differentiation Source: MGI
    94. spermatid differentiation Source: MGI
    95. spermatogenesis Source: MGI
    96. T cell homeostatic proliferation Source: MGI
    97. thymocyte apoptotic process Source: MGI
    98. transformed cell apoptotic process Source: UniProtKB
    99. vagina development Source: MGI

    Keywords - Biological processi

    Apoptosis

    Enzyme and pathway databases

    ReactomeiREACT_202639. Activation, translocation and oligomerization of BAX.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apoptosis regulator BAX
    Gene namesi
    Name:Bax
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:99702. Bax.

    Subcellular locationi

    Isoform Alpha : Mitochondrion outer membrane By similarity; Single-pass membrane protein By similarity. Cytoplasm By similarity
    Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane By similarity.By similarity
    Isoform Beta : Cytoplasm By similarity
    Isoform Gamma : Cytoplasm By similarity

    GO - Cellular componenti

    1. BAX complex Source: UniProtKB
    2. Bcl-2 family protein complex Source: UniProtKB
    3. cytoplasm Source: MGI
    4. cytosol Source: MGI
    5. endoplasmic reticulum Source: MGI
    6. endoplasmic reticulum membrane Source: UniProtKB
    7. integral component of membrane Source: MGI
    8. intracellular Source: MGI
    9. mitochondrial membrane Source: MGI
    10. mitochondrial outer membrane Source: MGI
    11. mitochondrial permeability transition pore complex Source: UniProtKB
    12. mitochondrion Source: MGI
    13. nucleus Source: UniProtKB
    14. pore complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 192192Apoptosis regulator BAXPRO_0000143057Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ07813.
    PaxDbiQ07813.
    PRIDEiQ07813.

    PTM databases

    PhosphoSiteiQ07813.

    Expressioni

    Tissue specificityi

    Expressed in a wide variety of tissues.

    Gene expression databases

    ArrayExpressiQ07813.
    BgeeiQ07813.
    CleanExiMM_BAX.
    GenevestigatoriQ07813.

    Interactioni

    Subunit structurei

    Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells By similarity. Interacts with FAIM2/LFG2 By similarity. Interacts with BOP By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Bcl2P104172EBI-700711,EBI-526314
    ERN1O754602EBI-700711,EBI-371750From a different organism.

    Protein-protein interaction databases

    BioGridi198304. 18 interactions.
    DIPiDIP-29541N.
    DIP-357N.
    IntActiQ07813. 8 interactions.
    MINTiMINT-1517262.

    Structurei

    Secondary structure

    1
    192
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi59 – 8022

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XA0X-ray2.70C/D52-82[»]
    ProteinModelPortaliQ07813.
    SMRiQ07813. Positions 1-192.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei172 – 19221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 7315BH3Add
    BLAST
    Motifi98 – 11821BH1Add
    BLAST
    Motifi150 – 16516BH2Add
    BLAST

    Domaini

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.By similarity

    Sequence similaritiesi

    Belongs to the Bcl-2 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46695.
    HOGENOMiHOG000095178.
    HOVERGENiHBG003606.
    InParanoidiQ07813.
    KOiK02159.
    OMAiADMFADG.
    OrthoDBiEOG70GMGD.
    PhylomeDBiQ07813.
    TreeFamiTF315834.

    Family and domain databases

    InterProiIPR026304. BAX.
    IPR002475. Bcl2-like.
    IPR020717. Bcl2_BH1_motif_CS.
    IPR020726. Bcl2_BH2_motif_CS.
    IPR020728. Bcl2_BH3_motif_CS.
    IPR026298. Blc2_fam.
    [Graphical view]
    PANTHERiPTHR11256. PTHR11256. 1 hit.
    PTHR11256:SF8. PTHR11256:SF8. 1 hit.
    PfamiPF00452. Bcl-2. 1 hit.
    [Graphical view]
    PRINTSiPR01862. BCL2FAMILY.
    PROSITEiPS50062. BCL2_FAMILY. 1 hit.
    PS01080. BH1. 1 hit.
    PS01258. BH2. 1 hit.
    PS01259. BH3. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform Alpha (identifier: Q07813-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDGSGEQLGS GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP    50
    PQDASTKKLS ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF 100
    ADGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLV 150
    WIQDQGGWEG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG 192
    Length:192
    Mass (Da):21,395
    Last modified:February 1, 1995 - v1
    Checksum:iD2E0B3566579FAFF
    GO
    Isoform Beta (identifier: Q07813-2)

    Sequence is not available
    Length:
    Mass (Da):
    Isoform Gamma (identifier: Q07813-3)

    Sequence is not available
    Length:
    Mass (Da):

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22472 mRNA. Translation: AAA03622.1.
    BC018228 mRNA. Translation: AAH18228.1.
    BC053380 mRNA. Translation: AAH53380.1.
    CCDSiCCDS21246.1. [Q07813-1]
    PIRiD47538.
    RefSeqiNP_031553.1. NM_007527.3. [Q07813-1]
    UniGeneiMm.19904.

    Genome annotation databases

    EnsembliENSMUST00000033093; ENSMUSP00000033093; ENSMUSG00000003873. [Q07813-1]
    GeneIDi12028.
    KEGGimmu:12028.
    UCSCiuc009gvh.1. mouse. [Q07813-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22472 mRNA. Translation: AAA03622.1 .
    BC018228 mRNA. Translation: AAH18228.1 .
    BC053380 mRNA. Translation: AAH53380.1 .
    CCDSi CCDS21246.1. [Q07813-1 ]
    PIRi D47538.
    RefSeqi NP_031553.1. NM_007527.3. [Q07813-1 ]
    UniGenei Mm.19904.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2XA0 X-ray 2.70 C/D 52-82 [» ]
    ProteinModelPortali Q07813.
    SMRi Q07813. Positions 1-192.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198304. 18 interactions.
    DIPi DIP-29541N.
    DIP-357N.
    IntActi Q07813. 8 interactions.
    MINTi MINT-1517262.

    PTM databases

    PhosphoSitei Q07813.

    Proteomic databases

    MaxQBi Q07813.
    PaxDbi Q07813.
    PRIDEi Q07813.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033093 ; ENSMUSP00000033093 ; ENSMUSG00000003873 . [Q07813-1 ]
    GeneIDi 12028.
    KEGGi mmu:12028.
    UCSCi uc009gvh.1. mouse. [Q07813-1 ]

    Organism-specific databases

    CTDi 581.
    MGIi MGI:99702. Bax.

    Phylogenomic databases

    eggNOGi NOG46695.
    HOGENOMi HOG000095178.
    HOVERGENi HBG003606.
    InParanoidi Q07813.
    KOi K02159.
    OMAi ADMFADG.
    OrthoDBi EOG70GMGD.
    PhylomeDBi Q07813.
    TreeFami TF315834.

    Enzyme and pathway databases

    Reactomei REACT_202639. Activation, translocation and oligomerization of BAX.

    Miscellaneous databases

    ChiTaRSi BAX. mouse.
    NextBioi 280259.
    PROi Q07813.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q07813.
    Bgeei Q07813.
    CleanExi MM_BAX.
    Genevestigatori Q07813.

    Family and domain databases

    InterProi IPR026304. BAX.
    IPR002475. Bcl2-like.
    IPR020717. Bcl2_BH1_motif_CS.
    IPR020726. Bcl2_BH2_motif_CS.
    IPR020728. Bcl2_BH3_motif_CS.
    IPR026298. Blc2_fam.
    [Graphical view ]
    PANTHERi PTHR11256. PTHR11256. 1 hit.
    PTHR11256:SF8. PTHR11256:SF8. 1 hit.
    Pfami PF00452. Bcl-2. 1 hit.
    [Graphical view ]
    PRINTSi PR01862. BCL2FAMILY.
    PROSITEi PS50062. BCL2_FAMILY. 1 hit.
    PS01080. BH1. 1 hit.
    PS01258. BH2. 1 hit.
    PS01259. BH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death."
      Oltvai Z.N., Milliman C.L., Korsmeyer S.J.
      Cell 74:609-619(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH BCL2, FUNCTION.
      Strain: C57BL/6 X DBA/2.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary tumor.
    3. "Evidence that inhibition of BAX activation by BCL-2 involves its tight and preferential interaction with the BH3 domain of BAX."
      Ku B., Liang C., Jung J.U., Oh B.H.
      Cell Res. 21:627-641(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 52-82 IN COMPLEX WITH BCL2, FUNCTION, INTERACTION WITH BCL2 AND BCL2L2.

    Entry informationi

    Entry nameiBAX_MOUSE
    AccessioniPrimary (citable) accession number: Q07813
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 151 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3