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Q07813

- BAX_MOUSE

UniProt

Q07813 - BAX_MOUSE

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Protein

Apoptosis regulator BAX

Gene

Bax

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production.2 Publications

GO - Molecular functioni

  1. channel activity Source: UniProtKB
  2. lipid binding Source: UniProtKB
  3. protein heterodimerization activity Source: MGI
  4. protein homodimerization activity Source: MGI

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  2. activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: MGI
  3. activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: MGI
  4. apoptotic mitochondrial changes Source: MGI
  5. apoptotic process Source: MGI
  6. apoptotic process involved in embryonic digit morphogenesis Source: MGI
  7. apoptotic process involved in patterning of blood vessels Source: MGI
  8. apoptotic signaling pathway Source: MGI
  9. B cell apoptotic process Source: MGI
  10. B cell homeostasis Source: MGI
  11. B cell homeostatic proliferation Source: MGI
  12. B cell negative selection Source: MGI
  13. B cell receptor apoptotic signaling pathway Source: Ensembl
  14. blood vessel remodeling Source: MGI
  15. cell proliferation Source: MGI
  16. cellular response to DNA damage stimulus Source: MGI
  17. cellular response to organic substance Source: MGI
  18. cellular response to UV Source: MGI
  19. cerebral cortex development Source: MGI
  20. development of secondary sexual characteristics Source: MGI
  21. ectopic germ cell programmed cell death Source: MGI
  22. establishment or maintenance of transmembrane electrochemical gradient Source: UniProtKB
  23. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  24. extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
  25. fertilization Source: MGI
  26. germ cell development Source: MGI
  27. glycosphingolipid metabolic process Source: MGI
  28. homeostasis of number of cells Source: MGI
  29. homeostasis of number of cells within a tissue Source: MGI
  30. hypothalamus development Source: MGI
  31. intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
  32. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  33. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
  34. kidney development Source: MGI
  35. leukocyte homeostasis Source: MGI
  36. limb morphogenesis Source: MGI
  37. male gonad development Source: MGI
  38. mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
  39. mitochondrial fusion Source: UniProtKB
  40. mitochondrion morphogenesis Source: MGI
  41. myeloid cell homeostasis Source: MGI
  42. negative regulation of apoptotic signaling pathway Source: MGI
  43. negative regulation of cell proliferation Source: MGI
  44. negative regulation of endoplasmic reticulum calcium ion concentration Source: MGI
  45. negative regulation of fibroblast proliferation Source: MGI
  46. negative regulation of neuron apoptotic process Source: MGI
  47. negative regulation of peptidyl-serine phosphorylation Source: MGI
  48. negative regulation of protein binding Source: UniProtKB
  49. nervous system development Source: MGI
  50. neuron apoptotic process Source: MGI
  51. neuron migration Source: MGI
  52. odontogenesis of dentin-containing tooth Source: MGI
  53. ovarian follicle development Source: MGI
  54. positive regulation of apoptotic DNA fragmentation Source: Ensembl
  55. positive regulation of apoptotic process Source: MGI
  56. positive regulation of apoptotic process involved in mammary gland involution Source: MGI
  57. positive regulation of B cell apoptotic process Source: MGI
  58. positive regulation of calcium ion transport into cytosol Source: MGI
  59. positive regulation of developmental pigmentation Source: MGI
  60. positive regulation of endoplasmic reticulum unfolded protein response Source: UniProtKB
  61. positive regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  62. positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
  63. positive regulation of mitochondrial membrane permeability involved in apoptotic process Source: MGI
  64. positive regulation of neuron apoptotic process Source: MGI
  65. positive regulation of protein oligomerization Source: UniProtKB
  66. positive regulation of release of cytochrome c from mitochondria Source: UniProtKB
  67. positive regulation of release of sequestered calcium ion into cytosol Source: MGI
  68. post-embryonic camera-type eye morphogenesis Source: MGI
  69. post-embryonic development Source: MGI
  70. protein homooligomerization Source: MGI
  71. protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: MGI
  72. regulation of cell cycle Source: MGI
  73. regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  74. regulation of mammary gland epithelial cell proliferation Source: MGI
  75. regulation of mitochondrial membrane permeability involved in apoptotic process Source: MGI
  76. regulation of mitochondrial membrane permeability involved in programmed necrotic cell death Source: MGI
  77. regulation of neuron apoptotic process Source: MGI
  78. regulation of nitrogen utilization Source: MGI
  79. regulation of protein heterodimerization activity Source: Ensembl
  80. regulation of protein homodimerization activity Source: UniProtKB
  81. release of cytochrome c from mitochondria Source: MGI
  82. release of matrix enzymes from mitochondria Source: UniProtKB
  83. response to axon injury Source: MGI
  84. response to gamma radiation Source: MGI
  85. response to ionizing radiation Source: MGI
  86. response to salt stress Source: MGI
  87. response to toxic substance Source: MGI
  88. response to wounding Source: MGI
  89. retina development in camera-type eye Source: MGI
  90. retinal cell apoptotic process Source: UniProtKB
  91. retinal cell programmed cell death Source: MGI
  92. Sertoli cell proliferation Source: MGI
  93. sex differentiation Source: MGI
  94. spermatid differentiation Source: MGI
  95. spermatogenesis Source: MGI
  96. T cell homeostatic proliferation Source: MGI
  97. thymocyte apoptotic process Source: MGI
  98. transformed cell apoptotic process Source: UniProtKB
  99. vagina development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiREACT_202639. Activation, translocation and oligomerization of BAX.

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis regulator BAX
Gene namesi
Name:Bax
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:99702. Bax.

Subcellular locationi

Isoform Alpha : Mitochondrion outer membrane By similarity; Single-pass membrane protein By similarity. Cytoplasm By similarity
Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane (By similarity).By similarity
Isoform Beta : Cytoplasm By similarity
Isoform Gamma : Cytoplasm By similarity

GO - Cellular componenti

  1. BAX complex Source: UniProtKB
  2. Bcl-2 family protein complex Source: UniProtKB
  3. cytoplasm Source: MGI
  4. cytosol Source: MGI
  5. endoplasmic reticulum Source: MGI
  6. endoplasmic reticulum membrane Source: UniProtKB
  7. extracellular vesicular exosome Source: Ensembl
  8. integral component of membrane Source: MGI
  9. intracellular Source: MGI
  10. mitochondrial membrane Source: MGI
  11. mitochondrial outer membrane Source: MGI
  12. mitochondrial permeability transition pore complex Source: UniProtKB
  13. mitochondrion Source: ParkinsonsUK-UCL
  14. nucleus Source: UniProtKB
  15. pore complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Apoptosis regulator BAXPRO_0000143057Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ07813.
PaxDbiQ07813.
PRIDEiQ07813.

PTM databases

PhosphoSiteiQ07813.

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues.

Gene expression databases

BgeeiQ07813.
CleanExiMM_BAX.
ExpressionAtlasiQ07813. baseline and differential.
GenevestigatoriQ07813.

Interactioni

Subunit structurei

Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells (By similarity). Interacts with FAIM2/LFG2 (By similarity). Interacts with BOP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Bcl2P104172EBI-700711,EBI-526314
ERN1O754602EBI-700711,EBI-371750From a different organism.

Protein-protein interaction databases

BioGridi198304. 18 interactions.
DIPiDIP-29541N.
DIP-357N.
IntActiQ07813. 8 interactions.
MINTiMINT-1517262.

Structurei

Secondary structure

1
192
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi59 – 8022

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XA0X-ray2.70C/D52-82[»]
ProteinModelPortaliQ07813.
SMRiQ07813. Positions 1-192.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 7315BH3Add
BLAST
Motifi98 – 11821BH1Add
BLAST
Motifi150 – 16516BH2Add
BLAST

Domaini

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.By similarity

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46695.
HOGENOMiHOG000095178.
HOVERGENiHBG003606.
InParanoidiQ07813.
KOiK02159.
OMAiADMFADG.
OrthoDBiEOG70GMGD.
PhylomeDBiQ07813.
TreeFamiTF315834.

Family and domain databases

InterProiIPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF8. PTHR11256:SF8. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform Alpha (identifier: Q07813-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGSGEQLGS GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP
60 70 80 90 100
PQDASTKKLS ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF
110 120 130 140 150
ADGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLV
160 170 180 190
WIQDQGGWEG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG
Length:192
Mass (Da):21,395
Last modified:February 1, 1995 - v1
Checksum:iD2E0B3566579FAFF
GO
Isoform Beta (identifier: Q07813-2)

Sequence is not available
Length:
Mass (Da):
Isoform Gamma (identifier: Q07813-3)

Sequence is not available
Length:
Mass (Da):

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22472 mRNA. Translation: AAA03622.1.
BC018228 mRNA. Translation: AAH18228.1.
BC053380 mRNA. Translation: AAH53380.1.
CCDSiCCDS21246.1. [Q07813-1]
PIRiD47538.
RefSeqiNP_031553.1. NM_007527.3. [Q07813-1]
UniGeneiMm.19904.

Genome annotation databases

EnsembliENSMUST00000033093; ENSMUSP00000033093; ENSMUSG00000003873. [Q07813-1]
GeneIDi12028.
KEGGimmu:12028.
UCSCiuc009gvh.1. mouse. [Q07813-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22472 mRNA. Translation: AAA03622.1 .
BC018228 mRNA. Translation: AAH18228.1 .
BC053380 mRNA. Translation: AAH53380.1 .
CCDSi CCDS21246.1. [Q07813-1 ]
PIRi D47538.
RefSeqi NP_031553.1. NM_007527.3. [Q07813-1 ]
UniGenei Mm.19904.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2XA0 X-ray 2.70 C/D 52-82 [» ]
ProteinModelPortali Q07813.
SMRi Q07813. Positions 1-192.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198304. 18 interactions.
DIPi DIP-29541N.
DIP-357N.
IntActi Q07813. 8 interactions.
MINTi MINT-1517262.

PTM databases

PhosphoSitei Q07813.

Proteomic databases

MaxQBi Q07813.
PaxDbi Q07813.
PRIDEi Q07813.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033093 ; ENSMUSP00000033093 ; ENSMUSG00000003873 . [Q07813-1 ]
GeneIDi 12028.
KEGGi mmu:12028.
UCSCi uc009gvh.1. mouse. [Q07813-1 ]

Organism-specific databases

CTDi 581.
MGIi MGI:99702. Bax.

Phylogenomic databases

eggNOGi NOG46695.
HOGENOMi HOG000095178.
HOVERGENi HBG003606.
InParanoidi Q07813.
KOi K02159.
OMAi ADMFADG.
OrthoDBi EOG70GMGD.
PhylomeDBi Q07813.
TreeFami TF315834.

Enzyme and pathway databases

Reactomei REACT_202639. Activation, translocation and oligomerization of BAX.

Miscellaneous databases

ChiTaRSi BAX. mouse.
NextBioi 280259.
PROi Q07813.
SOURCEi Search...

Gene expression databases

Bgeei Q07813.
CleanExi MM_BAX.
ExpressionAtlasi Q07813. baseline and differential.
Genevestigatori Q07813.

Family and domain databases

InterProi IPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view ]
PANTHERi PTHR11256. PTHR11256. 1 hit.
PTHR11256:SF8. PTHR11256:SF8. 1 hit.
Pfami PF00452. Bcl-2. 1 hit.
[Graphical view ]
PRINTSi PR01862. BCL2FAMILY.
PROSITEi PS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death."
    Oltvai Z.N., Milliman C.L., Korsmeyer S.J.
    Cell 74:609-619(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH BCL2, FUNCTION.
    Strain: C57BL/6 X DBA/2.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  3. "Evidence that inhibition of BAX activation by BCL-2 involves its tight and preferential interaction with the BH3 domain of BAX."
    Ku B., Liang C., Jung J.U., Oh B.H.
    Cell Res. 21:627-641(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 52-82 IN COMPLEX WITH BCL2, FUNCTION, INTERACTION WITH BCL2 AND BCL2L2.

Entry informationi

Entry nameiBAX_MOUSE
AccessioniPrimary (citable) accession number: Q07813
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3