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Q07813 (BAX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apoptosis regulator BAX
Gene names
Name:Bax
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length192 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production. Ref.1 Ref.3

Subunit structure

Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L) BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells By similarity. Interacts with FAIM2/LFG2 By similarity. Interacts with BOP By similarity. Ref.1 Ref.3

Subcellular location

Isoform Alpha: Mitochondrion outer membrane; Single-pass membrane protein By similarity. Cytoplasm By similarity. Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane By similarity.

Isoform Beta: Cytoplasm By similarity.

Isoform Gamma: Cytoplasm By similarity.

Tissue specificity

Expressed in a wide variety of tissues.

Domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family By similarity.

Sequence similarities

Belongs to the Bcl-2 family.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentCytoplasm
Membrane
Mitochondrion
Mitochondrion outer membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell apoptotic process

Inferred from genetic interaction PubMed 16055554. Source: MGI

B cell homeostasis

Inferred from mutant phenotype PubMed 16055554. Source: MGI

B cell homeostatic proliferation

Inferred from mutant phenotype PubMed 7569956. Source: MGI

B cell negative selection

Inferred from genetic interaction PubMed 16055554. Source: MGI

B cell receptor apoptotic signaling pathway

Inferred from electronic annotation. Source: Ensembl

Sertoli cell proliferation

Inferred from genetic interaction PubMed 11784036. Source: MGI

T cell homeostatic proliferation

Inferred from mutant phenotype PubMed 7569956PubMed 9241272. Source: MGI

activation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from direct assay PubMed 16735463. Source: UniProtKB

activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c

Inferred from direct assay PubMed 9560217. Source: MGI

activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway

Inferred from mutant phenotype PubMed 10479688. Source: MGI

apoptotic DNA fragmentation

Inferred from electronic annotation. Source: Ensembl

apoptotic mitochondrial changes

Inferred from direct assay PubMed 12925707. Source: MGI

apoptotic process

Inferred from direct assay PubMed 9560217. Source: MGI

apoptotic process involved in embryonic digit morphogenesis

Inferred from genetic interaction PubMed 15967824. Source: MGI

apoptotic process involved in patterning of blood vessels

Inferred from genetic interaction PubMed 15955981. Source: MGI

apoptotic signaling pathway

Inferred from mutant phenotype PubMed 12925707PubMed 14507967. Source: MGI

blood vessel remodeling

Inferred from genetic interaction PubMed 15955981. Source: MGI

cell proliferation

Inferred from direct assay PubMed 9176392. Source: MGI

cellular response to DNA damage stimulus

Inferred from mutant phenotype PubMed 12952892PubMed 9454845. Source: MGI

cellular response to UV

Inferred from genetic interaction PubMed 14963330. Source: MGI

cellular response to organic substance

Inferred from direct assay PubMed 12606450. Source: MGI

cerebral cortex development

Inferred from mutant phenotype PubMed 15342910. Source: MGI

development of secondary sexual characteristics

Inferred from mutant phenotype PubMed 15944391. Source: MGI

ectopic germ cell programmed cell death

Inferred from mutant phenotype PubMed 14660547. Source: MGI

establishment or maintenance of transmembrane electrochemical gradient

Inferred from sequence or structural similarity. Source: UniProtKB

extrinsic apoptotic signaling pathway in absence of ligand

Inferred from genetic interaction PubMed 15680329PubMed 15967824. Source: MGI

extrinsic apoptotic signaling pathway via death domain receptors

Inferred from direct assay PubMed 10597216. Source: MGI

fertilization

Inferred from genetic interaction PubMed 10894153. Source: MGI

germ cell development

Inferred from genetic interaction PubMed 10894153. Source: MGI

glycosphingolipid metabolic process

Inferred from mutant phenotype PubMed 18308723. Source: MGI

homeostasis of number of cells

Inferred from mutant phenotype PubMed 10931486. Source: MGI

homeostasis of number of cells within a tissue

Inferred from mutant phenotype PubMed 11413548PubMed 15342910. Source: MGI

hypothalamus development

Inferred from mutant phenotype PubMed 15342910. Source: MGI

intrinsic apoptotic signaling pathway by p53 class mediator

Inferred from mutant phenotype PubMed 10479688. Source: MGI

intrinsic apoptotic signaling pathway in response to DNA damage

Inferred from mutant phenotype PubMed 12952892. Source: MGI

intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress

Inferred from genetic interaction PubMed 12847083PubMed 15776018. Source: MGI

kidney development

Inferred from genetic interaction PubMed 9241272. Source: MGI

leukocyte homeostasis

Inferred from genetic interaction PubMed 11163212PubMed 15967824. Source: MGI

limb morphogenesis

Inferred from genetic interaction PubMed 11163212. Source: MGI

male gonad development

Inferred from mutant phenotype PubMed 16270031. Source: MGI

mitochondrial fragmentation involved in apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrial fusion

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrion morphogenesis

Inferred from genetic interaction PubMed 20671748. Source: MGI

myeloid cell homeostasis

Inferred from genetic interaction PubMed 15967824. Source: MGI

negative regulation of apoptotic signaling pathway

Inferred from genetic interaction PubMed 18436203. Source: MGI

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 10762311. Source: MGI

negative regulation of endoplasmic reticulum calcium ion concentration

Inferred from genetic interaction PubMed 15613488. Source: MGI

negative regulation of fibroblast proliferation

Inferred from mutant phenotype PubMed 12952892. Source: MGI

negative regulation of neuron apoptotic process

Inferred from direct assay PubMed 8625820. Source: MGI

negative regulation of peptidyl-serine phosphorylation

Inferred from genetic interaction PubMed 15613488. Source: MGI

negative regulation of protein binding

Inferred from sequence or structural similarity. Source: UniProtKB

nervous system development

Inferred from mutant phenotype PubMed 12810599. Source: MGI

neuron apoptotic process

Inferred from mutant phenotype PubMed 10479688PubMed 10618441PubMed 11226327PubMed 12917363PubMed 14623868PubMed 15574493. Source: MGI

neuron migration

Inferred from mutant phenotype PubMed 15590937. Source: MGI

nuclear fragmentation involved in apoptotic nuclear change

Inferred from electronic annotation. Source: Ensembl

odontogenesis of dentin-containing tooth

Inferred from genetic interaction PubMed 15967824. Source: MGI

ovarian follicle development

Inferred from mutant phenotype PubMed 17244737. Source: MGI

positive regulation of B cell apoptotic process

Inferred from genetic interaction PubMed 11604501. Source: MGI

positive regulation of apoptotic process

Inferred from direct assay PubMed 15753097. Source: MGI

positive regulation of apoptotic process involved in mammary gland involution

Inferred from mutant phenotype PubMed 10363969. Source: MGI

positive regulation of calcium ion transport into cytosol

Inferred from genetic interaction PubMed 15613488. Source: MGI

positive regulation of developmental pigmentation

Inferred from genetic interaction PubMed 9241272. Source: MGI

positive regulation of endoplasmic reticulum unfolded protein response

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of extrinsic apoptotic signaling pathway in absence of ligand

Inferred from direct assay Ref.1. Source: MGI

positive regulation of intrinsic apoptotic signaling pathway

Inferred from mutant phenotype PubMed 14502238. Source: MGI

positive regulation of mitochondrial membrane permeability involved in apoptotic process

Inferred from genetic interaction PubMed 15967824. Source: MGI

positive regulation of neuron apoptotic process

Inferred from mutant phenotype PubMed 10683286PubMed 10762311PubMed 11150333PubMed 14502238PubMed 15590937PubMed 15869495PubMed 15944391PubMed 15947791PubMed 16103918PubMed 17192424PubMed 8816704PubMed 9454852PubMed 9699561. Source: MGI

positive regulation of protein oligomerization

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of release of cytochrome c from mitochondria

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of release of sequestered calcium ion into cytosol

Inferred from mutant phenotype PubMed 15947791. Source: MGI

post-embryonic camera-type eye morphogenesis

Inferred from genetic interaction PubMed 15955981. Source: MGI

post-embryonic development

Inferred from genetic interaction PubMed 9241272. Source: MGI

protein homooligomerization

Inferred from direct assay PubMed 12925707. Source: MGI

protein insertion into mitochondrial membrane involved in apoptotic signaling pathway

Inferred from mutant phenotype PubMed 12952892. Source: MGI

regulation of cell cycle

Inferred from genetic interaction PubMed 16055554. Source: MGI

regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from mutant phenotype PubMed 14507967. Source: MGI

regulation of mammary gland epithelial cell proliferation

Inferred from mutant phenotype PubMed 10363969. Source: MGI

regulation of mitochondrial membrane permeability involved in apoptotic process

Inferred from genetic interaction PubMed 14963330. Source: MGI

regulation of mitochondrial membrane permeability involved in programmed necrotic cell death

Inferred from mutant phenotype PubMed 22493254. Source: MGI

regulation of neuron apoptotic process

Inferred from mutant phenotype PubMed 19029045. Source: MGI

regulation of nitrogen utilization

Inferred from genetic interaction PubMed 9241272. Source: MGI

regulation of protein heterodimerization activity

Inferred from electronic annotation. Source: Ensembl

regulation of protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

release of cytochrome c from mitochondria

Inferred from direct assay PubMed 12925707PubMed 14614769PubMed 14623868PubMed 9560217. Source: MGI

release of matrix enzymes from mitochondria

Inferred from sequence or structural similarity. Source: UniProtKB

response to axon injury

Inferred from mutant phenotype PubMed 16103918. Source: MGI

response to gamma radiation

Inferred from mutant phenotype PubMed 17068116. Source: MGI

response to ionizing radiation

Inferred from mutant phenotype PubMed 15578100. Source: MGI

response to salt stress

Inferred from genetic interaction PubMed 16571598. Source: MGI

response to toxic substance

Inferred from mutant phenotype PubMed 9454845. Source: MGI

response to wounding

Inferred from mutant phenotype PubMed 14507967. Source: MGI

retina development in camera-type eye

Inferred from mutant phenotype PubMed 9699561. Source: MGI

retinal cell apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

retinal cell programmed cell death

Inferred from mutant phenotype PubMed 14517994. Source: MGI

sex differentiation

Inferred from mutant phenotype PubMed 15342910. Source: MGI

spermatid differentiation

Inferred from mutant phenotype PubMed 16270031. Source: MGI

spermatogenesis

Inferred from mutant phenotype PubMed 11906913PubMed 7569956. Source: MGI

thymocyte apoptotic process

Inferred from genetic interaction PubMed 15967824. Source: MGI

transformed cell apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

vagina development

Inferred from genetic interaction PubMed 11163212. Source: MGI

   Cellular_componentBAX complex

Inferred from sequence or structural similarity. Source: UniProtKB

Bcl-2 family protein complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from direct assay PubMed 10464378PubMed 10486519PubMed 10931486PubMed 11226327PubMed 15528359PubMed 16469926. Source: MGI

cytosol

Inferred from direct assay PubMed 11369777PubMed 11864976PubMed 11980919PubMed 12847083PubMed 12925707PubMed 15304216PubMed 15989966PubMed 16227588PubMed 19139267PubMed 9553144. Source: MGI

endoplasmic reticulum

Inferred from direct assay PubMed 12847083. Source: MGI

endoplasmic reticulum membrane

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Inferred from sequence or structural similarity Ref.1. Source: MGI

intracellular

Inferred from direct assay PubMed 11146504PubMed 9008714. Source: MGI

mitochondrial membrane

Inferred from direct assay PubMed 11369777PubMed 11980919PubMed 14614769. Source: MGI

mitochondrial outer membrane

Inferred from direct assay PubMed 12910269PubMed 12925707PubMed 16227588. Source: MGI

mitochondrial permeability transition pore complex

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrion

Inferred from direct assay PubMed 11369777PubMed 11864976PubMed 12847083PubMed 14651853PubMed 15304216PubMed 15528359PubMed 16469926PubMed 18614015PubMed 19139267PubMed 20671748. Source: MGI

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

pore complex

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionchannel activity

Inferred from sequence or structural similarity. Source: UniProtKB

lipid binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction PubMed 11226327PubMed 7650488PubMed 7834748PubMed 8798452. Source: MGI

protein homodimerization activity

Inferred from mutant phenotype PubMed 8631771. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Bcl2P104172EBI-700711,EBI-526314
ERN1O754602EBI-700711,EBI-371750From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Select]
Isoform Alpha (identifier: Q07813-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: Q07813-2)

The sequence of this isoform is not available.
Isoform Gamma (identifier: Q07813-3)

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 192192Apoptosis regulator BAX
PRO_0000143057

Regions

Transmembrane172 – 19221Helical; Potential
Motif59 – 7315BH3
Motif98 – 11821BH1
Motif150 – 16516BH2

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Secondary structure

... 192
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: D2E0B3566579FAFF

FASTA19221,395
        10         20         30         40         50         60 
MDGSGEQLGS GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP PQDASTKKLS 

        70         80         90        100        110        120 
ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF ADGNFNWGRV VALFYFASKL 

       130        140        150        160        170        180 
VLKALCTKVP ELIRTIMGWT LDFLRERLLV WIQDQGGWEG LLSYFGTPTW QTVTIFVAGV 

       190 
LTASLTIWKK MG 

« Hide

Isoform Beta (Sequence not available).
Isoform Gamma (Sequence not available).

References

« Hide 'large scale' references
[1]"Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death."
Oltvai Z.N., Milliman C.L., Korsmeyer S.J.
Cell 74:609-619(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH BCL2, FUNCTION.
Strain: C57BL/6 X DBA/2.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Mammary tumor.
[3]"Evidence that inhibition of BAX activation by BCL-2 involves its tight and preferential interaction with the BH3 domain of BAX."
Ku B., Liang C., Jung J.U., Oh B.H.
Cell Res. 21:627-641(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 52-82 IN COMPLEX WITH BCL2, FUNCTION, INTERACTION WITH BCL2 AND BCL2L2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L22472 mRNA. Translation: AAA03622.1.
BC018228 mRNA. Translation: AAH18228.1.
BC053380 mRNA. Translation: AAH53380.1.
PIRD47538.
RefSeqNP_031553.1. NM_007527.3.
UniGeneMm.19904.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2XA0X-ray2.70C/D52-82[»]
ProteinModelPortalQ07813.
SMRQ07813. Positions 1-192.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198304. 17 interactions.
DIPDIP-29541N.
DIP-357N.
IntActQ07813. 8 interactions.
MINTMINT-1517262.

PTM databases

PhosphoSiteQ07813.

Proteomic databases

PaxDbQ07813.
PRIDEQ07813.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033093; ENSMUSP00000033093; ENSMUSG00000003873. [Q07813-1]
GeneID12028.
KEGGmmu:12028.
UCSCuc009gvh.1. mouse. [Q07813-1]

Organism-specific databases

CTD581.
MGIMGI:99702. Bax.

Phylogenomic databases

eggNOGNOG46695.
HOGENOMHOG000095178.
HOVERGENHBG003606.
InParanoidQ07813.
KOK02159.
OMAADMFADG.
OrthoDBEOG70GMGD.
PhylomeDBQ07813.
TreeFamTF315834.

Gene expression databases

ArrayExpressQ07813.
BgeeQ07813.
CleanExMM_BAX.
GenevestigatorQ07813.

Family and domain databases

InterProIPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF9. PTHR11256:SF9. 1 hit.
PfamPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSPR01862. BCL2FAMILY.
PROSITEPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSBAX. mouse.
NextBio280259.
PROQ07813.
SOURCESearch...

Entry information

Entry nameBAX_MOUSE
AccessionPrimary (citable) accession number: Q07813
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot