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Protein

Penicillin-binding protein 1A

Gene

mrcA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei461 – 4611Acyl-ester intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP-1a
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcA
Synonyms:ponA
Ordered Locus Names:PA5045
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5045.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55CytoplasmicSequence analysis
Transmembranei6 – 2621Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini27 – 822796PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2363020.
DrugBankiDB01329. Cefoperazone.
DB00430. Cefpiramide.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 822822Penicillin-binding protein 1APRO_0000083173Add
BLAST

Proteomic databases

PaxDbiQ07806.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5045.

Chemistry

BindingDBiQ07806.

Structurei

Secondary structure

1
822
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi48 – 514Combined sources
Beta strandi57 – 615Combined sources
Helixi262 – 27615Combined sources
Helixi279 – 2824Combined sources
Beta strandi286 – 2905Combined sources
Helixi293 – 31220Combined sources
Beta strandi320 – 3223Combined sources
Helixi328 – 3369Combined sources
Beta strandi344 – 3529Combined sources
Beta strandi354 – 3607Combined sources
Beta strandi366 – 3705Combined sources
Helixi371 – 3744Combined sources
Beta strandi382 – 3843Combined sources
Helixi393 – 3964Combined sources
Beta strandi401 – 4077Combined sources
Beta strandi413 – 4164Combined sources
Beta strandi420 – 4289Combined sources
Turni430 – 4323Combined sources
Beta strandi434 – 4396Combined sources
Turni444 – 4463Combined sources
Turni451 – 4533Combined sources
Helixi460 – 4634Combined sources
Helixi464 – 4729Combined sources
Turni473 – 4753Combined sources
Beta strandi481 – 4855Combined sources
Beta strandi508 – 5125Combined sources
Helixi513 – 5186Combined sources
Helixi522 – 53211Combined sources
Helixi534 – 5429Combined sources
Turni543 – 5453Combined sources
Helixi548 – 5503Combined sources
Helixi555 – 5595Combined sources
Helixi566 – 57712Combined sources
Beta strandi588 – 5925Combined sources
Beta strandi594 – 5963Combined sources
Beta strandi598 – 6014Combined sources
Helixi662 – 67716Combined sources
Helixi682 – 6876Combined sources
Beta strandi693 – 6986Combined sources
Helixi700 – 7023Combined sources
Beta strandi704 – 7107Combined sources
Beta strandi715 – 7217Combined sources
Helixi734 – 74916Combined sources
Beta strandi764 – 7696Combined sources
Turni771 – 7733Combined sources
Beta strandi774 – 7763Combined sources
Beta strandi785 – 7906Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OONX-ray3.20A36-822[»]
ProteinModelPortaliQ07806.
SMRiQ07806. Positions 47-792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 216169TransglycosylaseAdd
BLAST
Regioni403 – 744342TranspeptidaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JQC. Bacteria.
COG5009. LUCA.
HOGENOMiHOG000041138.
InParanoidiQ07806.
KOiK05366.
OMAiIAQVWVT.
PhylomeDBiQ07806.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.710.10. 3 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR012340. NA-bd_OB-fold.
IPR031376. PCB_OB.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF17092. PCB_OB. 1 hit.
PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 4 hits.

Sequencei

Sequence statusi: Complete.

Q07806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLKFLWWT CVTLICGVLL SFSGAYLYLS PSLPSVEALR NVQLQIPLKV
60 70 80 90 100
YSEDGKLISE FGEMRRTPIR FADIPQDFIH ALLSAEDDNF ANHYGVDVKS
110 120 130 140 150
LMRAAAQLLK SGHIQTGGST ITMQVAKNYF LTNERSFSRK INEILLALQI
160 170 180 190 200
ERQLTKDEIL ELYVNKIYLG NRAYGIEAAA QVYYGKPIKD LSLAEMAMIA
210 220 230 240 250
GLPKAPSRYN PLVNPTRSTE RRNWILERML KLGFIDQQRY QAAVEEPINA
260 270 280 290 300
SYHVQTPELN APYIAEMARA EMVGRYGSEA YTEGYKVITT VRSDLQNAAS
310 320 330 340 350
QSVRDGLIDY DQRHGYRGPE TRLPGQTRDA WLKHLGQQRS IGGLEPAIVT
360 370 380 390 400
QVEKSGIMVM TRDGKEEAVT WDSMKWARPF LSNNSMGPMP RQPADVAQAG
410 420 430 440 450
DQIRVQRQED GTLRFVQIPA AQSALISLDP KDGAIRSLVG GFSFEQSNYN
460 470 480 490 500
RAIQAKRQPG SSFKPFIYSA ALDNGFTAAS LVNDAPIVFV DEYLDKVWRP
510 520 530 540 550
KNDTNTFLGP IPLREALYKS RNMVSIRVLQ GLGIERAISY ITKFGFQRDE
560 570 580 590 600
LPRNFSLALG TATVTPMEIA GAWSVFANGG YKVNPYVIER IESRDGQVLY
610 620 630 640 650
QANPPRVPVE EQVAADAEDA GNPGDPEHPE SAEGEGSIEA QQVAAKAQTT
660 670 680 690 700
FEPTPAERII DARTAYIMTS MLQDVIKRGT GRRALALKRT DLAGKTGTTN
710 720 730 740 750
DSKDGWFSGY NSDYVTSVWV GFDQPETLGR REYGGTVALP IWIRYMGFAL
760 770 780 790 800
KDKPMHTMAE PPGIVSLRID PVTGRSAAPG TPGAYFEMFK NEDTPPSVNE
810 820
LPPGSFPGSP LPDDEGAPID LF
Length:822
Mass (Da):91,200
Last modified:January 1, 1998 - v2
Checksum:i0B1A3F6FA5D492AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73780 Genomic DNA. Translation: AAB39710.1.
AE004091 Genomic DNA. Translation: AAG08430.1.
L13867 Unassigned DNA. Translation: AAC36826.1.
PIRiF83016.
RefSeqiNP_253732.1. NC_002516.2.
WP_003114576.1. NZ_ASJY01000799.1.

Genome annotation databases

EnsemblBacteriaiAAG08430; AAG08430; PA5045.
GeneIDi881163.
KEGGipae:PA5045.
PATRICi19844921. VBIPseAer58763_5289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73780 Genomic DNA. Translation: AAB39710.1.
AE004091 Genomic DNA. Translation: AAG08430.1.
L13867 Unassigned DNA. Translation: AAC36826.1.
PIRiF83016.
RefSeqiNP_253732.1. NC_002516.2.
WP_003114576.1. NZ_ASJY01000799.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OONX-ray3.20A36-822[»]
ProteinModelPortaliQ07806.
SMRiQ07806. Positions 47-792.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5045.

Chemistry

BindingDBiQ07806.
ChEMBLiCHEMBL2363020.
DrugBankiDB01329. Cefoperazone.
DB00430. Cefpiramide.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Proteomic databases

PaxDbiQ07806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08430; AAG08430; PA5045.
GeneIDi881163.
KEGGipae:PA5045.
PATRICi19844921. VBIPseAer58763_5289.

Organism-specific databases

PseudoCAPiPA5045.

Phylogenomic databases

eggNOGiENOG4108JQC. Bacteria.
COG5009. LUCA.
HOGENOMiHOG000041138.
InParanoidiQ07806.
KOiK05366.
OMAiIAQVWVT.
PhylomeDBiQ07806.

Enzyme and pathway databases

UniPathwayiUPA00219.

Miscellaneous databases

PROiQ07806.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.710.10. 3 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR012340. NA-bd_OB-fold.
IPR031376. PCB_OB.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF17092. PCB_OB. 1 hit.
PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 4 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPBPA_PSEAE
AccessioniPrimary (citable) accession number: Q07806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.