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Protein

Penicillin-binding protein 1A

Gene

mrcA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei461Acyl-ester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP-1a
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcA
Synonyms:ponA
Ordered Locus Names:PA5045
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5045.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5CytoplasmicSequence analysis5
Transmembranei6 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini27 – 822PeriplasmicSequence analysisAdd BLAST796

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3259512.
DrugBankiDB01329. Cefoperazone.
DB00430. Cefpiramide.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000831731 – 822Penicillin-binding protein 1AAdd BLAST822

Proteomic databases

PaxDbiQ07806.
PRIDEiQ07806.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5045.

Chemistry databases

BindingDBiQ07806.

Structurei

Secondary structure

1822
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi48 – 51Combined sources4
Beta strandi57 – 61Combined sources5
Helixi262 – 276Combined sources15
Helixi279 – 282Combined sources4
Beta strandi286 – 290Combined sources5
Helixi293 – 312Combined sources20
Beta strandi320 – 322Combined sources3
Helixi328 – 336Combined sources9
Beta strandi344 – 352Combined sources9
Beta strandi354 – 360Combined sources7
Beta strandi366 – 370Combined sources5
Helixi371 – 374Combined sources4
Beta strandi382 – 384Combined sources3
Helixi393 – 396Combined sources4
Beta strandi401 – 407Combined sources7
Beta strandi413 – 416Combined sources4
Beta strandi420 – 428Combined sources9
Turni430 – 432Combined sources3
Beta strandi434 – 439Combined sources6
Turni444 – 446Combined sources3
Turni451 – 453Combined sources3
Helixi460 – 463Combined sources4
Helixi464 – 472Combined sources9
Turni473 – 475Combined sources3
Beta strandi481 – 485Combined sources5
Beta strandi508 – 512Combined sources5
Helixi513 – 518Combined sources6
Helixi522 – 532Combined sources11
Helixi534 – 542Combined sources9
Turni543 – 545Combined sources3
Helixi548 – 550Combined sources3
Helixi555 – 559Combined sources5
Helixi566 – 577Combined sources12
Beta strandi588 – 592Combined sources5
Beta strandi594 – 596Combined sources3
Beta strandi598 – 601Combined sources4
Helixi662 – 677Combined sources16
Helixi682 – 687Combined sources6
Beta strandi693 – 698Combined sources6
Helixi700 – 702Combined sources3
Beta strandi704 – 710Combined sources7
Beta strandi715 – 721Combined sources7
Helixi734 – 749Combined sources16
Beta strandi764 – 769Combined sources6
Turni771 – 773Combined sources3
Beta strandi774 – 776Combined sources3
Beta strandi785 – 790Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OONX-ray3.20A36-822[»]
ProteinModelPortaliQ07806.
SMRiQ07806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 216TransglycosylaseAdd BLAST169
Regioni403 – 744TranspeptidaseAdd BLAST342

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JQC. Bacteria.
COG5009. LUCA.
HOGENOMiHOG000041138.
InParanoidiQ07806.
KOiK05366.
OMAiIAQVWVT.
PhylomeDBiQ07806.

Family and domain databases

Gene3Di3.40.710.10. 3 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR031376. PCB_OB.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF17092. PCB_OB. 1 hit.
PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 2 hits.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 4 hits.

Sequencei

Sequence statusi: Complete.

Q07806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLKFLWWT CVTLICGVLL SFSGAYLYLS PSLPSVEALR NVQLQIPLKV
60 70 80 90 100
YSEDGKLISE FGEMRRTPIR FADIPQDFIH ALLSAEDDNF ANHYGVDVKS
110 120 130 140 150
LMRAAAQLLK SGHIQTGGST ITMQVAKNYF LTNERSFSRK INEILLALQI
160 170 180 190 200
ERQLTKDEIL ELYVNKIYLG NRAYGIEAAA QVYYGKPIKD LSLAEMAMIA
210 220 230 240 250
GLPKAPSRYN PLVNPTRSTE RRNWILERML KLGFIDQQRY QAAVEEPINA
260 270 280 290 300
SYHVQTPELN APYIAEMARA EMVGRYGSEA YTEGYKVITT VRSDLQNAAS
310 320 330 340 350
QSVRDGLIDY DQRHGYRGPE TRLPGQTRDA WLKHLGQQRS IGGLEPAIVT
360 370 380 390 400
QVEKSGIMVM TRDGKEEAVT WDSMKWARPF LSNNSMGPMP RQPADVAQAG
410 420 430 440 450
DQIRVQRQED GTLRFVQIPA AQSALISLDP KDGAIRSLVG GFSFEQSNYN
460 470 480 490 500
RAIQAKRQPG SSFKPFIYSA ALDNGFTAAS LVNDAPIVFV DEYLDKVWRP
510 520 530 540 550
KNDTNTFLGP IPLREALYKS RNMVSIRVLQ GLGIERAISY ITKFGFQRDE
560 570 580 590 600
LPRNFSLALG TATVTPMEIA GAWSVFANGG YKVNPYVIER IESRDGQVLY
610 620 630 640 650
QANPPRVPVE EQVAADAEDA GNPGDPEHPE SAEGEGSIEA QQVAAKAQTT
660 670 680 690 700
FEPTPAERII DARTAYIMTS MLQDVIKRGT GRRALALKRT DLAGKTGTTN
710 720 730 740 750
DSKDGWFSGY NSDYVTSVWV GFDQPETLGR REYGGTVALP IWIRYMGFAL
760 770 780 790 800
KDKPMHTMAE PPGIVSLRID PVTGRSAAPG TPGAYFEMFK NEDTPPSVNE
810 820
LPPGSFPGSP LPDDEGAPID LF
Length:822
Mass (Da):91,200
Last modified:January 1, 1998 - v2
Checksum:i0B1A3F6FA5D492AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73780 Genomic DNA. Translation: AAB39710.1.
AE004091 Genomic DNA. Translation: AAG08430.1.
L13867 Unassigned DNA. Translation: AAC36826.1.
PIRiF83016.
RefSeqiNP_253732.1. NC_002516.2.
WP_003114576.1. NZ_ASJY01000799.1.

Genome annotation databases

EnsemblBacteriaiAAG08430; AAG08430; PA5045.
GeneIDi881163.
KEGGipae:PA5045.
PATRICi19844921. VBIPseAer58763_5289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73780 Genomic DNA. Translation: AAB39710.1.
AE004091 Genomic DNA. Translation: AAG08430.1.
L13867 Unassigned DNA. Translation: AAC36826.1.
PIRiF83016.
RefSeqiNP_253732.1. NC_002516.2.
WP_003114576.1. NZ_ASJY01000799.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OONX-ray3.20A36-822[»]
ProteinModelPortaliQ07806.
SMRiQ07806.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5045.

Chemistry databases

BindingDBiQ07806.
ChEMBLiCHEMBL3259512.
DrugBankiDB01329. Cefoperazone.
DB00430. Cefpiramide.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Proteomic databases

PaxDbiQ07806.
PRIDEiQ07806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08430; AAG08430; PA5045.
GeneIDi881163.
KEGGipae:PA5045.
PATRICi19844921. VBIPseAer58763_5289.

Organism-specific databases

PseudoCAPiPA5045.

Phylogenomic databases

eggNOGiENOG4108JQC. Bacteria.
COG5009. LUCA.
HOGENOMiHOG000041138.
InParanoidiQ07806.
KOiK05366.
OMAiIAQVWVT.
PhylomeDBiQ07806.

Enzyme and pathway databases

UniPathwayiUPA00219.

Miscellaneous databases

PROiQ07806.

Family and domain databases

Gene3Di3.40.710.10. 3 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR031376. PCB_OB.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF17092. PCB_OB. 1 hit.
PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 2 hits.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 4 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPBPA_PSEAE
AccessioniPrimary (citable) accession number: Q07806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.