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Protein

Sterol esterase 1

Gene

YEH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the hydrolysis of steryl esters, thereby playing a central role in lipid metabolism. Under heme-deficient conditions, it constitutes the major steryl ester hydrolase, suggesting that it plays a central role in mobilization of steryl esters under anaerobic conditions.2 Publications

Catalytic activityi

A steryl ester + H2O = a sterol + a fatty acid.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei315NucleophileBy similarity1
Active sitei489Charge relay systemBy similarity1
Active sitei520Charge relay systemBy similarity1

GO - Molecular functioni

  • sterol esterase activity Source: SGD

GO - Biological processi

  • lipid catabolic process Source: UniProtKB-KW
  • sterol metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32117-MONOMER.
YEAST:G3O-32117-MONOMER.
BRENDAi3.1.1.13. 984.

Protein family/group databases

ESTHERiyeast-YLL012W. Acidic_Lipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol esterase 1 (EC:3.1.1.13)
Alternative name(s):
Steryl ester hydrolase 1
Gene namesi
Name:YEH1
Ordered Locus Names:YLL012W
ORF Names:L1329
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL012W.
SGDiS000003935. YEH1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Intramembranei13 – 33Sequence analysisAdd BLAST21
Topological domaini34 – 573CytoplasmicSequence analysisAdd BLAST540

GO - Cellular componenti

  • integral component of membrane Source: SGD
  • lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002484041 – 573Sterol esterase 1Add BLAST573

Post-translational modificationi

Not N-glycosylated.

Proteomic databases

MaxQBiQ07804.
PRIDEiQ07804.

PTM databases

iPTMnetiQ07804.

Expressioni

Inductioni

Under heme-deficiency conditions. Heme-deficient induction requires ROX3.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi31239. 34 interactors.
DIPiDIP-4156N.
IntActiQ07804. 13 interactors.
MINTiMINT-544351.

Structurei

3D structure databases

ProteinModelPortaliQ07804.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00550000076072.
HOGENOMiHOG000141749.
InParanoidiQ07804.
OMAiWAHDVIE.
OrthoDBiEOG092C16RM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR006693. AB_hydrolase_lipase.
[Graphical view]
PfamiPF04083. Abhydro_lipase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q07804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVSAVLKRA RNLLATFIVC CFMAVVLVLA LAHHFINEHR DTRSSSTQIE
60 70 80 90 100
VDDESKRNVH HDHVLTRTNA YATPYLDLEH DKKNGIVYDH TRTVVRKKNH
110 120 130 140 150
EVGSSSLHKN LFHKFLTKLI FRFIEKEKVT EGVTQGKFNN SSNEIANHEP
160 170 180 190 200
VFEKIPVQCD NPLQNLILSE DLTLVADLNY YFNQYNIQIE EFRLETEDGF
210 220 230 240 250
VIDLWHLIPK YRTTDSDKKK RPPILMLHGL LQSSGSFASN GRKSLAYFLY
260 270 280 290 300
QSGYDIWLGN NRCGFRPEWN EAKVPTLASR WDWDLREMVK YDLTLLIDTV
310 320 330 340 350
LAKTQFEKLT LISHSQGTTQ GFMGLVNEDK FFPPGSGSKE SFFTSKIANY
360 370 380 390 400
IALAPAVYPG PLLNEKLFVK LMTKEIENPW FFGETSFFEI MMIVRNLCVG
410 420 430 440 450
ESLFSFVCYT IFNYLFDWND TLWDTALRDR HFLFSPVHVS VKLMQWWLSP
460 470 480 490 500
DPNKVSFKFG SHKMFPDNVK WFSDASKAPN IYLFVPKQDR LVDGERLINH
510 520 530 540 550
FVNVESNVNY KIWYIDEYAH IDVLWAHDVI ERIGKPILQN LNNYYSKKPS
560 570
SAFESDCSDT EVETELEMVA EKA
Length:573
Mass (Da):66,508
Last modified:November 1, 1996 - v1
Checksum:iB679123D8733F48A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73117 Genomic DNA. Translation: CAA97456.1.
X91488 Genomic DNA. Translation: CAA62780.1.
BK006945 Genomic DNA. Translation: DAA09306.1.
PIRiS64754.
RefSeqiNP_013089.1. NM_001181832.1.

Genome annotation databases

EnsemblFungiiYLL012W; YLL012W; YLL012W.
GeneIDi850648.
KEGGisce:YLL012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73117 Genomic DNA. Translation: CAA97456.1.
X91488 Genomic DNA. Translation: CAA62780.1.
BK006945 Genomic DNA. Translation: DAA09306.1.
PIRiS64754.
RefSeqiNP_013089.1. NM_001181832.1.

3D structure databases

ProteinModelPortaliQ07804.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31239. 34 interactors.
DIPiDIP-4156N.
IntActiQ07804. 13 interactors.
MINTiMINT-544351.

Protein family/group databases

ESTHERiyeast-YLL012W. Acidic_Lipase.

PTM databases

iPTMnetiQ07804.

Proteomic databases

MaxQBiQ07804.
PRIDEiQ07804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL012W; YLL012W; YLL012W.
GeneIDi850648.
KEGGisce:YLL012W.

Organism-specific databases

EuPathDBiFungiDB:YLL012W.
SGDiS000003935. YEH1.

Phylogenomic databases

GeneTreeiENSGT00550000076072.
HOGENOMiHOG000141749.
InParanoidiQ07804.
OMAiWAHDVIE.
OrthoDBiEOG092C16RM.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32117-MONOMER.
YEAST:G3O-32117-MONOMER.
BRENDAi3.1.1.13. 984.

Miscellaneous databases

PROiQ07804.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR006693. AB_hydrolase_lipase.
[Graphical view]
PfamiPF04083. Abhydro_lipase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYEH1_YEAST
AccessioniPrimary (citable) accession number: Q07804
Secondary accession number(s): D6VXZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.