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Protein

Sterol esterase 1

Gene

YEH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the hydrolysis of steryl esters, thereby playing a central role in lipid metabolism. Under heme-deficient conditions, it constitutes the major steryl ester hydrolase, suggesting that it plays a central role in mobilization of steryl esters under anaerobic conditions.2 Publications

Miscellaneous

Present with 7770 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

A steryl ester + H2O = a sterol + a fatty acid.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei315NucleophileBy similarity1
Active sitei489Charge relay systemBy similarity1
Active sitei520Charge relay systemBy similarity1

GO - Molecular functioni

  • sterol esterase activity Source: SGD

GO - Biological processi

  • lipid catabolic process Source: UniProtKB-KW
  • sterol metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32117-MONOMER
YEAST:G3O-32117-MONOMER
BRENDAi3.1.1.13 984

Protein family/group databases

ESTHERiyeast-YLL012W Acidic_Lipase

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol esterase 1 (EC:3.1.1.13)
Alternative name(s):
Steryl ester hydrolase 1
Gene namesi
Name:YEH1
Ordered Locus Names:YLL012W
ORF Names:L1329
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL012W
SGDiS000003935 YEH1

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Intramembranei13 – 33Sequence analysisAdd BLAST21
Topological domaini34 – 573CytoplasmicSequence analysisAdd BLAST540

Keywords - Cellular componenti

Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002484041 – 573Sterol esterase 1Add BLAST573

Post-translational modificationi

Not N-glycosylated.

Proteomic databases

MaxQBiQ07804
PaxDbiQ07804
PRIDEiQ07804

PTM databases

iPTMnetiQ07804

Expressioni

Inductioni

Under heme-deficiency conditions. Heme-deficient induction requires ROX3.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi31239, 54 interactors
DIPiDIP-4156N
IntActiQ07804, 14 interactors
MINTiQ07804
STRINGi4932.YLL012W

Structurei

3D structure databases

ProteinModelPortaliQ07804
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Phylogenomic databases

HOGENOMiHOG000141749
InParanoidiQ07804
OMAiRIEWERW
OrthoDBiEOG092C16RM

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR006693 AB_hydrolase_lipase
PfamiView protein in Pfam
PF04083 Abhydro_lipase, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

Q07804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVSAVLKRA RNLLATFIVC CFMAVVLVLA LAHHFINEHR DTRSSSTQIE
60 70 80 90 100
VDDESKRNVH HDHVLTRTNA YATPYLDLEH DKKNGIVYDH TRTVVRKKNH
110 120 130 140 150
EVGSSSLHKN LFHKFLTKLI FRFIEKEKVT EGVTQGKFNN SSNEIANHEP
160 170 180 190 200
VFEKIPVQCD NPLQNLILSE DLTLVADLNY YFNQYNIQIE EFRLETEDGF
210 220 230 240 250
VIDLWHLIPK YRTTDSDKKK RPPILMLHGL LQSSGSFASN GRKSLAYFLY
260 270 280 290 300
QSGYDIWLGN NRCGFRPEWN EAKVPTLASR WDWDLREMVK YDLTLLIDTV
310 320 330 340 350
LAKTQFEKLT LISHSQGTTQ GFMGLVNEDK FFPPGSGSKE SFFTSKIANY
360 370 380 390 400
IALAPAVYPG PLLNEKLFVK LMTKEIENPW FFGETSFFEI MMIVRNLCVG
410 420 430 440 450
ESLFSFVCYT IFNYLFDWND TLWDTALRDR HFLFSPVHVS VKLMQWWLSP
460 470 480 490 500
DPNKVSFKFG SHKMFPDNVK WFSDASKAPN IYLFVPKQDR LVDGERLINH
510 520 530 540 550
FVNVESNVNY KIWYIDEYAH IDVLWAHDVI ERIGKPILQN LNNYYSKKPS
560 570
SAFESDCSDT EVETELEMVA EKA
Length:573
Mass (Da):66,508
Last modified:November 1, 1996 - v1
Checksum:iB679123D8733F48A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73117 Genomic DNA Translation: CAA97456.1
X91488 Genomic DNA Translation: CAA62780.1
BK006945 Genomic DNA Translation: DAA09306.1
PIRiS64754
RefSeqiNP_013089.1, NM_001181832.1

Genome annotation databases

EnsemblFungiiYLL012W; YLL012W; YLL012W
GeneIDi850648
KEGGisce:YLL012W

Similar proteinsi

Entry informationi

Entry nameiYEH1_YEAST
AccessioniPrimary (citable) accession number: Q07804
Secondary accession number(s): D6VXZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health