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Protein

Elongation factor G, mitochondrial

Gene

Gfm1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.UniRule annotation

Pathwayi: polypeptide chain elongation

This protein is involved in the pathway polypeptide chain elongation, which is part of Protein biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway polypeptide chain elongation and in Protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi54 – 618GTPUniRule annotation
Nucleotide bindingi121 – 1255GTPUniRule annotation
Nucleotide bindingi175 – 1784GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-5389840. Mitochondrial translation elongation.
UniPathwayiUPA00345.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor G, mitochondrialUniRule annotation
Short name:
EF-GmtUniRule annotation
Alternative name(s):
Elongation factor G 1, mitochondrialUniRule annotation
Short name:
mEF-G 1UniRule annotation
Elongation factor G1UniRule annotation
Gene namesi
Name:Gfm1
Synonyms:Efg, Efg1, Gfm
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi631396. Gfm1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737MitochondrionUniRule annotationAdd
BLAST
Chaini38 – 751714Elongation factor G, mitochondrialPRO_0000007443Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei92 – 921PhosphoserineBy similarity
Modified residuei176 – 1761N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ07803.
PRIDEiQ07803.

PTM databases

iPTMnetiQ07803.

Expressioni

Tissue specificityi

Detected in all tissues with the highest level in liver, thymus and brain.

Gene expression databases

ExpressionAtlasiQ07803. baseline and differential.
GenevisibleiQ07803. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040081.

Structurei

3D structure databases

ProteinModelPortaliQ07803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 322278tr-type GAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0465. Eukaryota.
COG0480. LUCA.
GeneTreeiENSGT00550000074911.
HOGENOMiHOG000231585.
InParanoidiQ07803.
KOiK02355.
OMAiKLGVAIQ.
OrthoDBiEOG7VDXNM.
PhylomeDBiQ07803.
TreeFamiTF105631.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLRITAGL GRGPLPRVPA ILGWQGKQAN WKTYRWCSSG SIPNEKIRNI
60 70 80 90 100
GISAHIDSGK TTLTERVLYY TGRIATMHEV KGKDGVGAVM DSMELERQRG
110 120 130 140 150
ITIQSAATYT MWRDVNINII DTPGHVDFTI EVERALRVLD GAVLVLCAVG
160 170 180 190 200
GVQCQTMTVS RQMKRYNVPF LTFINKLDRM GSNPARALQQ MRSKLNHNAA
210 220 230 240 250
FVQIPIGLEG DFKGIIDLIE ERAIYFDGDF GQIVRYDEIP ADLRAAAADH
260 270 280 290 300
RQELIECVAN SDEQLGELFL EEKIPSVSDL KLAIRRATLS RSFTPVFLGS
310 320 330 340 350
ALKNKGVQPL LDAVLEFLPN PSEVQNYALL NQNDSKEKNK ILMNPKRDDS
360 370 380 390 400
HPFVGLAFKL EAGRFGQLTY VRNYQGELKK GSTIYNTRTG KKVRVQRLVR
410 420 430 440 450
MHADMMEDVE EVYAGDICAL FGIDCASGDT FTNKDNSDLS MESIHVPDPV
460 470 480 490 500
ISVAMKPSNK NDLEKFSKGI ARFTREDPTF KVHFDTESKE TIVSGMGELH
510 520 530 540 550
LEIYAQRMER EYGCPCITGK PKVAFRETVT APVPFDFTHK KQSGGAGQYG
560 570 580 590 600
KVIGVLEPLA PEDYTKLEFS DETFGANVPK QFVPAVEKGF LDACEKGPLS
610 620 630 640 650
GHKLSGLRFV LQDGAHHMVD SNEISFIRAG EGALKQALAS ATLCIIEPIM
660 670 680 690 700
SVEVIAPNEF QGAVFAGINR RHGVITGQDG IEDYFTLYAD VPLNNMFGYS
710 720 730 740 750
TELRSCTEGK GEYTMEYNRY QPCSPSTQEE LVNKYLEATG QLPVKKGKAK

N
Length:751
Mass (Da):83,457
Last modified:October 13, 2009 - v2
Checksum:iA4DD3BD637B295E1
GO

Sequence cautioni

The sequence AAA41107.1 differs from that shown. Reason: Frameshift at positions 488, 502, 521, 524 and 550. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 2521Q → P in AAA41107 (PubMed:8332461).Curated
Sequence conflicti306 – 3061G → R in AAA41107 (PubMed:8332461).Curated
Sequence conflicti335 – 3351S → L in AAA41107 (PubMed:8332461).Curated
Sequence conflicti339 – 3391N → K in AAA41107 (PubMed:8332461).Curated
Sequence conflicti564 – 5652YT → LP in AAA41107 (PubMed:8332461).Curated
Sequence conflicti739 – 7391T → I in AAA41107 (PubMed:8332461).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14684 mRNA. Translation: AAA41107.1. Frameshift.
CH473976 Genomic DNA. Translation: EDM00923.1.
BC085721 mRNA. Translation: AAH85721.1.
PIRiS40780.
RefSeqiNP_446077.1. NM_053625.1.
UniGeneiRn.10913.

Genome annotation databases

EnsembliENSRNOT00000042079; ENSRNOP00000040081; ENSRNOG00000012873.
GeneIDi114017.
KEGGirno:114017.
UCSCiRGD:631396. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14684 mRNA. Translation: AAA41107.1. Frameshift.
CH473976 Genomic DNA. Translation: EDM00923.1.
BC085721 mRNA. Translation: AAH85721.1.
PIRiS40780.
RefSeqiNP_446077.1. NM_053625.1.
UniGeneiRn.10913.

3D structure databases

ProteinModelPortaliQ07803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040081.

PTM databases

iPTMnetiQ07803.

Proteomic databases

PaxDbiQ07803.
PRIDEiQ07803.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000042079; ENSRNOP00000040081; ENSRNOG00000012873.
GeneIDi114017.
KEGGirno:114017.
UCSCiRGD:631396. rat.

Organism-specific databases

CTDi85476.
RGDi631396. Gfm1.

Phylogenomic databases

eggNOGiKOG0465. Eukaryota.
COG0480. LUCA.
GeneTreeiENSGT00550000074911.
HOGENOMiHOG000231585.
InParanoidiQ07803.
KOiK02355.
OMAiKLGVAIQ.
OrthoDBiEOG7VDXNM.
PhylomeDBiQ07803.
TreeFamiTF105631.

Enzyme and pathway databases

UniPathwayiUPA00345.
ReactomeiR-RNO-5389840. Mitochondrial translation elongation.

Miscellaneous databases

PROiQ07803.

Gene expression databases

ExpressionAtlasiQ07803. baseline and differential.
GenevisibleiQ07803. RN.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the gene encoding the mitochondrial elongation factor G in mammals."
    Barker C.S., Makris A., Patriotis C., Bear S.E., Tsichlis P.N.
    Nucleic Acids Res. 21:2641-2647(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  4. Lubec G., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 214-222 AND 292-303, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiEFGM_RAT
AccessioniPrimary (citable) accession number: Q07803
Secondary accession number(s): Q5U347
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 13, 2009
Last modified: June 8, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.