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Protein

Transcription factor COE1

Gene

Ebf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri151 – 170C5-typeSequence analysisAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor COE1
Short name:
O/E-1
Short name:
OE-1
Alternative name(s):
Early B-cell factor
Gene namesi
Name:Ebf1
Synonyms:Coe1, Ebf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:95275. Ebf1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63R → A: Strongly reduced interaction with DNA. 1 Publication1
Mutagenesisi66N → A: Reduced interaction with DNA. 1 Publication1
Mutagenesisi163R → A: Strongly reduced interaction with DNA. 1 Publication1
Mutagenesisi203G → E: Strongly reduced interaction with DNA. 1 Publication1
Mutagenesisi235H → A: Strongly reduced interaction with DNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078261 – 591Transcription factor COE1Add BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki16Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki16Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ07802.
PRIDEiQ07802.

PTM databases

PhosphoSitePlusiQ07802.

Expressioni

Tissue specificityi

Expressed at high levels in early B-cells, spleen, lymph node and adipose tissue, and at low levels in heart, brain, skeletal muscle and kidney. In adult expressed in olfactory epithelium, in spleen, and at a lesser extent in Purkinje cells of the cerebellum, heart, kidney, lung, thymus and testis. In embryo expressed in dorsal thalamus and epithalamus, at a lower level in mesencephalon and in the caudal rhombencephalon, in the postmitotic cells of developing retina, highly in developing spinal cord, dorsal root ganglia, trigeminal ganglia and in glossopharyngeal nerve ganglia, in developing inner ear.

Developmental stagei

First detected at 9.5 dpc.

Gene expression databases

BgeeiENSMUSG00000057098.
CleanExiMM_EBF1.
ExpressionAtlasiQ07802. baseline and differential.
GenevisibleiQ07802. MM.

Interactioni

Subunit structurei

Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes (By similarity). Forms either a homodimer or a heterodimer with a related family member.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei163Interaction with DNA1
Sitei172Interaction with DNA1

Protein-protein interaction databases

BioGridi199358. 1 interactor.
STRINGi10090.ENSMUSP00000080020.

Structurei

Secondary structure

1591
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 38Combined sources3
Helixi39 – 44Combined sources6
Beta strandi48 – 56Combined sources9
Beta strandi60 – 63Combined sources4
Beta strandi66 – 76Combined sources11
Beta strandi83 – 93Combined sources11
Helixi95 – 97Combined sources3
Helixi99 – 101Combined sources3
Beta strandi108 – 115Combined sources8
Beta strandi121 – 132Combined sources12
Turni133 – 135Combined sources3
Helixi148 – 150Combined sources3
Beta strandi152 – 155Combined sources4
Helixi157 – 160Combined sources4
Helixi162 – 165Combined sources4
Helixi171 – 175Combined sources5
Beta strandi181 – 183Combined sources3
Turni184 – 186Combined sources3
Beta strandi187 – 194Combined sources8
Beta strandi201 – 204Combined sources4
Beta strandi211 – 219Combined sources9
Beta strandi221 – 228Combined sources8
Beta strandi232 – 235Combined sources4
Turni238 – 241Combined sources4
Turni249 – 251Combined sources3
Beta strandi263 – 266Combined sources4
Beta strandi271 – 273Combined sources3
Beta strandi279 – 285Combined sources7
Beta strandi292 – 295Combined sources4
Beta strandi298 – 300Combined sources3
Beta strandi302 – 306Combined sources5
Beta strandi309 – 313Combined sources5
Beta strandi321 – 329Combined sources9
Beta strandi332 – 335Combined sources4
Beta strandi340 – 345Combined sources6
Turni351 – 361Combined sources11
Helixi375 – 389Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MLNX-ray2.40A/B/E24-241[»]
3MLOX-ray3.01A/B24-241[»]
3MLPX-ray2.80A/B/E/F24-421[»]
ProteinModelPortaliQ07802.
SMRiQ07802.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07802.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini262 – 345IPT/TIGAdd BLAST84

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 66Interaction with DNA4
Regioni197 – 204Interaction with DNA8
Regioni236 – 239Interaction with DNA4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi462 – 550Pro/Ser/Thr-richAdd BLAST89

Sequence similaritiesi

Belongs to the COE family.Curated
Contains 1 IPT/TIG domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri151 – 170C5-typeSequence analysisAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3836. Eukaryota.
ENOG410XQ9Z. LUCA.
GeneTreeiENSGT00390000014051.
HOGENOMiHOG000092311.
HOVERGENiHBG005108.
InParanoidiQ07802.
KOiK09103.
TreeFamiTF313391.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR032200. COE_DBD.
IPR032201. COE_HLH.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]
PANTHERiPTHR10747. PTHR10747. 1 hit.
PfamiPF16422. COE1_DBD. 1 hit.
PF16423. COE1_HLH. 1 hit.
PF01833. TIG. 1 hit.
[Graphical view]
SMARTiSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS01345. COE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q07802-1) [UniParc]FASTAAdd to basket
Also known as: 8

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA
60 70 80 90 100
RAHFEKQPPS NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN
110 120 130 140 150
SEKTNNGIHY RLQLLYSNGI RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM
160 170 180 190 200
CRVLLTHEIM CSRCCDKKSC GNRNETPSDP VIIDRFFLKF FLKCNQNCLK
210 220 230 240 250
NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS
260 270 280 290 300
EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV
310 320 330 340 350
WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP
360 370 380 390 400
TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII
410 420 430 440 450
LKRAADIAEA LYSVPRNHNQ LPALANTSVH AGMMGVNSFS GQLAVNVSEA
460 470 480 490 500
SQATNQGFTR NSSSVSPHGY VPSTTPQQTN YNSVTTSMNG YGSAAMSNLG
510 520 530 540 550
GSPTFLNGSA ANSPYAIVPS SPTMASSTSL PSNCSSSSGI FSFSPANMVS
560 570 580 590
AVKQKSAFAP VVRPQTSPPP TCTSTNGNSL QAISGMIVPP M
Length:591
Mass (Da):64,464
Last modified:November 1, 1996 - v1
Checksum:iE47797B6FC1E5071
GO
Isoform Short (identifier: Q07802-2) [UniParc]FASTAAdd to basket
Also known as: O

The sequence of this isoform differs from the canonical sequence as follows:
     252-259: Missing.

Show »
Length:583
Mass (Da):63,579
Checksum:i81A00C02F4986723
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87T → F AA sequence (PubMed:8491377).Curated1
Sequence conflicti89F → L AA sequence (PubMed:8491377).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001112252 – 259Missing in isoform Short. Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12147 mRNA. Translation: AAA37533.1.
CCDSiCCDS24566.1. [Q07802-1]
PIRiA40684.
RefSeqiNP_031923.1. NM_007897.3. [Q07802-1]
XP_006532219.1. XM_006532156.3. [Q07802-2]
UniGeneiMm.439662.
Mm.440346.

Genome annotation databases

EnsembliENSMUST00000081265; ENSMUSP00000080020; ENSMUSG00000057098. [Q07802-1]
GeneIDi13591.
KEGGimmu:13591.
UCSCiuc007ink.2. mouse. [Q07802-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12147 mRNA. Translation: AAA37533.1.
CCDSiCCDS24566.1. [Q07802-1]
PIRiA40684.
RefSeqiNP_031923.1. NM_007897.3. [Q07802-1]
XP_006532219.1. XM_006532156.3. [Q07802-2]
UniGeneiMm.439662.
Mm.440346.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MLNX-ray2.40A/B/E24-241[»]
3MLOX-ray3.01A/B24-241[»]
3MLPX-ray2.80A/B/E/F24-421[»]
ProteinModelPortaliQ07802.
SMRiQ07802.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199358. 1 interactor.
STRINGi10090.ENSMUSP00000080020.

PTM databases

PhosphoSitePlusiQ07802.

Proteomic databases

PaxDbiQ07802.
PRIDEiQ07802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081265; ENSMUSP00000080020; ENSMUSG00000057098. [Q07802-1]
GeneIDi13591.
KEGGimmu:13591.
UCSCiuc007ink.2. mouse. [Q07802-1]

Organism-specific databases

CTDi1879.
MGIiMGI:95275. Ebf1.

Phylogenomic databases

eggNOGiKOG3836. Eukaryota.
ENOG410XQ9Z. LUCA.
GeneTreeiENSGT00390000014051.
HOGENOMiHOG000092311.
HOVERGENiHBG005108.
InParanoidiQ07802.
KOiK09103.
TreeFamiTF313391.

Enzyme and pathway databases

ReactomeiR-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSiEbf1. mouse.
EvolutionaryTraceiQ07802.
PROiQ07802.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057098.
CleanExiMM_EBF1.
ExpressionAtlasiQ07802. baseline and differential.
GenevisibleiQ07802. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR032200. COE_DBD.
IPR032201. COE_HLH.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]
PANTHERiPTHR10747. PTHR10747. 1 hit.
PfamiPF16422. COE1_DBD. 1 hit.
PF16423. COE1_HLH. 1 hit.
PF01833. TIG. 1 hit.
[Graphical view]
SMARTiSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS01345. COE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOE1_MOUSE
AccessioniPrimary (citable) accession number: Q07802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.