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Q07802

- COE1_MOUSE

UniProt

Q07802 - COE1_MOUSE

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Protein
Transcription factor COE1
Gene
Ebf1, Coe1, Ebf
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei163 – 1631Interaction with DNA
Sitei172 – 1721Interaction with DNA

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri151 – 17020C5-type Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: MGI
  4. sequence-specific DNA binding transcription factor activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. positive regulation of transcription, DNA-templated Source: MGI
  3. regulation of transcription, DNA-templated Source: MGI
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor COE1
Short name:
O/E-1
Short name:
OE-1
Alternative name(s):
Early B-cell factor
Gene namesi
Name:Ebf1
Synonyms:Coe1, Ebf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:95275. Ebf1.

Subcellular locationi

Nucleus Reviewed prediction

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi63 – 631R → A: Strongly reduced interaction with DNA. 1 Publication
Mutagenesisi66 – 661N → A: Reduced interaction with DNA. 1 Publication
Mutagenesisi163 – 1631R → A: Strongly reduced interaction with DNA. 1 Publication
Mutagenesisi203 – 2031G → E: Strongly reduced interaction with DNA. 1 Publication
Mutagenesisi235 – 2351H → A: Strongly reduced interaction with DNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591Transcription factor COE1
PRO_0000107826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ07802.
PRIDEiQ07802.

PTM databases

PhosphoSiteiQ07802.

Expressioni

Tissue specificityi

Expressed at high levels in early B-cells, spleen, lymph node and adipose tissue, and at low levels in heart, brain, skeletal muscle and kidney. In adult expressed in olfactory epithelium, in spleen, and at a lesser extent in Purkinje cells of the cerebellum, heart, kidney, lung, thymus and testis. In embryo expressed in dorsal thalamus and epithalamus, at a lower level in mesencephalon and in the caudal rhombencephalon, in the postmitotic cells of developing retina, highly in developing spinal cord, dorsal root ganglia, trigeminal ganglia and in glossopharyngeal nerve ganglia, in developing inner ear.

Developmental stagei

First detected at 9.5 dpc.

Gene expression databases

ArrayExpressiQ07802.
BgeeiQ07802.
CleanExiMM_EBF1.
GenevestigatoriQ07802.

Interactioni

Subunit structurei

Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes By similarity. Forms either a homodimer or a heterodimer with a related family member.2 Publications

Protein-protein interaction databases

BioGridi199358. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 383
Helixi39 – 446
Beta strandi48 – 569
Beta strandi60 – 634
Beta strandi66 – 7611
Beta strandi83 – 9311
Helixi95 – 973
Helixi99 – 1013
Beta strandi108 – 1158
Beta strandi121 – 13212
Turni133 – 1353
Helixi148 – 1503
Beta strandi152 – 1554
Helixi157 – 1604
Helixi162 – 1654
Helixi171 – 1755
Beta strandi181 – 1833
Turni184 – 1863
Beta strandi187 – 1948
Beta strandi201 – 2044
Beta strandi211 – 2199
Beta strandi221 – 2288
Beta strandi232 – 2354
Turni238 – 2414
Turni249 – 2513
Beta strandi263 – 2664
Beta strandi271 – 2733
Beta strandi279 – 2857
Beta strandi292 – 2954
Beta strandi298 – 3003
Beta strandi302 – 3065
Beta strandi309 – 3135
Beta strandi321 – 3299
Beta strandi332 – 3354
Beta strandi340 – 3456
Turni351 – 36111
Helixi375 – 38915

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MLNX-ray2.40A/B/E24-241[»]
3MLOX-ray3.01A/B24-241[»]
3MLPX-ray2.80A/B/E/F24-421[»]
ProteinModelPortaliQ07802.
SMRiQ07802. Positions 35-413.

Miscellaneous databases

EvolutionaryTraceiQ07802.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini262 – 34584IPT/TIG
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 664Interaction with DNA
Regioni197 – 2048Interaction with DNA
Regioni236 – 2394Interaction with DNA

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi462 – 55089Pro/Ser/Thr-rich
Add
BLAST

Sequence similaritiesi

Belongs to the COE family.
Contains 1 IPT/TIG domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG259050.
HOGENOMiHOG000092311.
HOVERGENiHBG005108.
KOiK09103.
OrthoDBiEOG7J446V.
TreeFamiTF313391.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]
PANTHERiPTHR10747. PTHR10747. 1 hit.
PfamiPF01833. TIG. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS01345. COE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q07802-1) [UniParc]FASTAAdd to Basket

Also known as: 8

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA    50
RAHFEKQPPS NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN 100
SEKTNNGIHY RLQLLYSNGI RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM 150
CRVLLTHEIM CSRCCDKKSC GNRNETPSDP VIIDRFFLKF FLKCNQNCLK 200
NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS 250
EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV 300
WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP 350
TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII 400
LKRAADIAEA LYSVPRNHNQ LPALANTSVH AGMMGVNSFS GQLAVNVSEA 450
SQATNQGFTR NSSSVSPHGY VPSTTPQQTN YNSVTTSMNG YGSAAMSNLG 500
GSPTFLNGSA ANSPYAIVPS SPTMASSTSL PSNCSSSSGI FSFSPANMVS 550
AVKQKSAFAP VVRPQTSPPP TCTSTNGNSL QAISGMIVPP M 591
Length:591
Mass (Da):64,464
Last modified:November 1, 1996 - v1
Checksum:iE47797B6FC1E5071
GO
Isoform Short (identifier: Q07802-2) [UniParc]FASTAAdd to Basket

Also known as: O

The sequence of this isoform differs from the canonical sequence as follows:
     252-259: Missing.

Show »
Length:583
Mass (Da):63,579
Checksum:i81A00C02F4986723
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei252 – 2598Missing in isoform Short.
VSP_001112

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871T → F AA sequence 1 Publication
Sequence conflicti89 – 891F → L AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12147 mRNA. Translation: AAA37533.1.
CCDSiCCDS24566.1. [Q07802-1]
PIRiA40684.
RefSeqiNP_031923.1. NM_007897.3. [Q07802-1]
XP_006532219.1. XM_006532156.1. [Q07802-2]
UniGeneiMm.439662.
Mm.440346.

Genome annotation databases

EnsembliENSMUST00000081265; ENSMUSP00000080020; ENSMUSG00000057098. [Q07802-1]
GeneIDi13591.
KEGGimmu:13591.
UCSCiuc007ink.1. mouse. [Q07802-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12147 mRNA. Translation: AAA37533.1 .
CCDSi CCDS24566.1. [Q07802-1 ]
PIRi A40684.
RefSeqi NP_031923.1. NM_007897.3. [Q07802-1 ]
XP_006532219.1. XM_006532156.1. [Q07802-2 ]
UniGenei Mm.439662.
Mm.440346.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MLN X-ray 2.40 A/B/E 24-241 [» ]
3MLO X-ray 3.01 A/B 24-241 [» ]
3MLP X-ray 2.80 A/B/E/F 24-421 [» ]
ProteinModelPortali Q07802.
SMRi Q07802. Positions 35-413.
ModBasei Search...

Protein-protein interaction databases

BioGridi 199358. 1 interaction.

PTM databases

PhosphoSitei Q07802.

Proteomic databases

PaxDbi Q07802.
PRIDEi Q07802.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081265 ; ENSMUSP00000080020 ; ENSMUSG00000057098 . [Q07802-1 ]
GeneIDi 13591.
KEGGi mmu:13591.
UCSCi uc007ink.1. mouse. [Q07802-1 ]

Organism-specific databases

CTDi 1879.
MGIi MGI:95275. Ebf1.

Phylogenomic databases

eggNOGi NOG259050.
HOGENOMi HOG000092311.
HOVERGENi HBG005108.
KOi K09103.
OrthoDBi EOG7J446V.
TreeFami TF313391.

Enzyme and pathway databases

Reactomei REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSi EBF1. mouse.
EvolutionaryTracei Q07802.
NextBioi 284202.
PROi Q07802.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q07802.
Bgeei Q07802.
CleanExi MM_EBF1.
Genevestigatori Q07802.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
InterProi IPR011598. bHLH_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view ]
PANTHERi PTHR10747. PTHR10747. 1 hit.
Pfami PF01833. TIG. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
SM00429. IPT. 1 hit.
[Graphical view ]
SUPFAMi SSF81296. SSF81296. 1 hit.
PROSITEi PS01345. COE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and functional characterization of early B-cell factor, a regulator of lymphocyte-specific gene expression."
    Hagman J., Belanger C., Travis A., Turck W., Grosschedl R.
    Genes Dev. 7:760-773(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 87-99; 122-129; 342-358 AND 382-390.
    Tissue: Lymphoid tissue.
  2. "Purification of early-B-cell factor and characterization of its DNA-binding specificity."
    Travis A., Hagman J., Hwang L., Grosschedl R.
    Mol. Cell. Biol. 13:3392-3400(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  3. "The characterization of the Olf-1/EBF-like HLH transcription factor family: implications in olfactory gene regulation and neuronal development."
    Wang S.S., Tsai R.Y.L., Reed R.R.
    J. Neurosci. 17:4149-4158(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, ALTERNATIVE SPLICING.
    Strain: CD-1.
    Tissue: Embryo.
  4. "Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins."
    Treiber N., Treiber T., Zocher G., Grosschedl R.
    Genes Dev. 24:2270-2275(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 24-241 IN COMPLEX WITH TARGET DNA, FUNCTION, SUBUNIT, MUTAGENESIS OF ARG-63; ASN-66; ARG-163; GLY-203 AND HIS-235.

Entry informationi

Entry nameiCOE1_MOUSE
AccessioniPrimary (citable) accession number: Q07802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi