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Q07802

- COE1_MOUSE

UniProt

Q07802 - COE1_MOUSE

Protein

Transcription factor COE1

Gene

Ebf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei163 – 1631Interaction with DNA
    Sitei172 – 1721Interaction with DNA

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri151 – 17020C5-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. positive regulation of transcription, DNA-templated Source: MGI
    3. regulation of transcription, DNA-templated Source: MGI
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor COE1
    Short name:
    O/E-1
    Short name:
    OE-1
    Alternative name(s):
    Early B-cell factor
    Gene namesi
    Name:Ebf1
    Synonyms:Coe1, Ebf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:95275. Ebf1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleoplasm Source: Reactome
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi63 – 631R → A: Strongly reduced interaction with DNA. 1 Publication
    Mutagenesisi66 – 661N → A: Reduced interaction with DNA. 1 Publication
    Mutagenesisi163 – 1631R → A: Strongly reduced interaction with DNA. 1 Publication
    Mutagenesisi203 – 2031G → E: Strongly reduced interaction with DNA. 1 Publication
    Mutagenesisi235 – 2351H → A: Strongly reduced interaction with DNA. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 591591Transcription factor COE1PRO_0000107826Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ07802.
    PRIDEiQ07802.

    PTM databases

    PhosphoSiteiQ07802.

    Expressioni

    Tissue specificityi

    Expressed at high levels in early B-cells, spleen, lymph node and adipose tissue, and at low levels in heart, brain, skeletal muscle and kidney. In adult expressed in olfactory epithelium, in spleen, and at a lesser extent in Purkinje cells of the cerebellum, heart, kidney, lung, thymus and testis. In embryo expressed in dorsal thalamus and epithalamus, at a lower level in mesencephalon and in the caudal rhombencephalon, in the postmitotic cells of developing retina, highly in developing spinal cord, dorsal root ganglia, trigeminal ganglia and in glossopharyngeal nerve ganglia, in developing inner ear.

    Developmental stagei

    First detected at 9.5 dpc.

    Gene expression databases

    ArrayExpressiQ07802.
    BgeeiQ07802.
    CleanExiMM_EBF1.
    GenevestigatoriQ07802.

    Interactioni

    Subunit structurei

    Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes By similarity. Forms either a homodimer or a heterodimer with a related family member.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi199358. 1 interaction.

    Structurei

    Secondary structure

    1
    591
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 383
    Helixi39 – 446
    Beta strandi48 – 569
    Beta strandi60 – 634
    Beta strandi66 – 7611
    Beta strandi83 – 9311
    Helixi95 – 973
    Helixi99 – 1013
    Beta strandi108 – 1158
    Beta strandi121 – 13212
    Turni133 – 1353
    Helixi148 – 1503
    Beta strandi152 – 1554
    Helixi157 – 1604
    Helixi162 – 1654
    Helixi171 – 1755
    Beta strandi181 – 1833
    Turni184 – 1863
    Beta strandi187 – 1948
    Beta strandi201 – 2044
    Beta strandi211 – 2199
    Beta strandi221 – 2288
    Beta strandi232 – 2354
    Turni238 – 2414
    Turni249 – 2513
    Beta strandi263 – 2664
    Beta strandi271 – 2733
    Beta strandi279 – 2857
    Beta strandi292 – 2954
    Beta strandi298 – 3003
    Beta strandi302 – 3065
    Beta strandi309 – 3135
    Beta strandi321 – 3299
    Beta strandi332 – 3354
    Beta strandi340 – 3456
    Turni351 – 36111
    Helixi375 – 38915

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3MLNX-ray2.40A/B/E24-241[»]
    3MLOX-ray3.01A/B24-241[»]
    3MLPX-ray2.80A/B/E/F24-421[»]
    ProteinModelPortaliQ07802.
    SMRiQ07802. Positions 35-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ07802.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini262 – 34584IPT/TIGAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 664Interaction with DNA
    Regioni197 – 2048Interaction with DNA
    Regioni236 – 2394Interaction with DNA

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi462 – 55089Pro/Ser/Thr-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the COE family.Curated
    Contains 1 IPT/TIG domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri151 – 17020C5-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG259050.
    HOGENOMiHOG000092311.
    HOVERGENiHBG005108.
    KOiK09103.
    OrthoDBiEOG7J446V.
    TreeFamiTF313391.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002909. IPT.
    IPR003523. Transcription_factor_COE.
    IPR018350. Transcription_factor_COE_CS.
    [Graphical view]
    PANTHERiPTHR10747. PTHR10747. 1 hit.
    PfamiPF01833. TIG. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    SM00429. IPT. 1 hit.
    [Graphical view]
    SUPFAMiSSF81296. SSF81296. 1 hit.
    PROSITEiPS01345. COE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q07802-1) [UniParc]FASTAAdd to Basket

    Also known as: 8

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA    50
    RAHFEKQPPS NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN 100
    SEKTNNGIHY RLQLLYSNGI RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM 150
    CRVLLTHEIM CSRCCDKKSC GNRNETPSDP VIIDRFFLKF FLKCNQNCLK 200
    NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS 250
    EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV 300
    WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP 350
    TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII 400
    LKRAADIAEA LYSVPRNHNQ LPALANTSVH AGMMGVNSFS GQLAVNVSEA 450
    SQATNQGFTR NSSSVSPHGY VPSTTPQQTN YNSVTTSMNG YGSAAMSNLG 500
    GSPTFLNGSA ANSPYAIVPS SPTMASSTSL PSNCSSSSGI FSFSPANMVS 550
    AVKQKSAFAP VVRPQTSPPP TCTSTNGNSL QAISGMIVPP M 591
    Length:591
    Mass (Da):64,464
    Last modified:November 1, 1996 - v1
    Checksum:iE47797B6FC1E5071
    GO
    Isoform Short (identifier: Q07802-2) [UniParc]FASTAAdd to Basket

    Also known as: O

    The sequence of this isoform differs from the canonical sequence as follows:
         252-259: Missing.

    Show »
    Length:583
    Mass (Da):63,579
    Checksum:i81A00C02F4986723
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti87 – 871T → F AA sequence (PubMed:8491377)Curated
    Sequence conflicti89 – 891F → L AA sequence (PubMed:8491377)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei252 – 2598Missing in isoform Short. CuratedVSP_001112

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12147 mRNA. Translation: AAA37533.1.
    CCDSiCCDS24566.1. [Q07802-1]
    PIRiA40684.
    RefSeqiNP_031923.1. NM_007897.3. [Q07802-1]
    XP_006532219.1. XM_006532156.1. [Q07802-2]
    UniGeneiMm.439662.
    Mm.440346.

    Genome annotation databases

    EnsembliENSMUST00000081265; ENSMUSP00000080020; ENSMUSG00000057098. [Q07802-1]
    GeneIDi13591.
    KEGGimmu:13591.
    UCSCiuc007ink.1. mouse. [Q07802-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12147 mRNA. Translation: AAA37533.1 .
    CCDSi CCDS24566.1. [Q07802-1 ]
    PIRi A40684.
    RefSeqi NP_031923.1. NM_007897.3. [Q07802-1 ]
    XP_006532219.1. XM_006532156.1. [Q07802-2 ]
    UniGenei Mm.439662.
    Mm.440346.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3MLN X-ray 2.40 A/B/E 24-241 [» ]
    3MLO X-ray 3.01 A/B 24-241 [» ]
    3MLP X-ray 2.80 A/B/E/F 24-421 [» ]
    ProteinModelPortali Q07802.
    SMRi Q07802. Positions 35-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199358. 1 interaction.

    PTM databases

    PhosphoSitei Q07802.

    Proteomic databases

    PaxDbi Q07802.
    PRIDEi Q07802.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081265 ; ENSMUSP00000080020 ; ENSMUSG00000057098 . [Q07802-1 ]
    GeneIDi 13591.
    KEGGi mmu:13591.
    UCSCi uc007ink.1. mouse. [Q07802-1 ]

    Organism-specific databases

    CTDi 1879.
    MGIi MGI:95275. Ebf1.

    Phylogenomic databases

    eggNOGi NOG259050.
    HOGENOMi HOG000092311.
    HOVERGENi HBG005108.
    KOi K09103.
    OrthoDBi EOG7J446V.
    TreeFami TF313391.

    Enzyme and pathway databases

    Reactomei REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Miscellaneous databases

    ChiTaRSi EBF1. mouse.
    EvolutionaryTracei Q07802.
    NextBioi 284202.
    PROi Q07802.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q07802.
    Bgeei Q07802.
    CleanExi MM_EBF1.
    Genevestigatori Q07802.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002909. IPT.
    IPR003523. Transcription_factor_COE.
    IPR018350. Transcription_factor_COE_CS.
    [Graphical view ]
    PANTHERi PTHR10747. PTHR10747. 1 hit.
    Pfami PF01833. TIG. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    SM00429. IPT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81296. SSF81296. 1 hit.
    PROSITEi PS01345. COE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional characterization of early B-cell factor, a regulator of lymphocyte-specific gene expression."
      Hagman J., Belanger C., Travis A., Turck W., Grosschedl R.
      Genes Dev. 7:760-773(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 87-99; 122-129; 342-358 AND 382-390.
      Tissue: Lymphoid tissue.
    2. "Purification of early-B-cell factor and characterization of its DNA-binding specificity."
      Travis A., Hagman J., Hwang L., Grosschedl R.
      Mol. Cell. Biol. 13:3392-3400(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    3. "The characterization of the Olf-1/EBF-like HLH transcription factor family: implications in olfactory gene regulation and neuronal development."
      Wang S.S., Tsai R.Y.L., Reed R.R.
      J. Neurosci. 17:4149-4158(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, ALTERNATIVE SPLICING.
      Strain: CD-1.
      Tissue: Embryo.
    4. "Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins."
      Treiber N., Treiber T., Zocher G., Grosschedl R.
      Genes Dev. 24:2270-2275(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 24-241 IN COMPLEX WITH TARGET DNA, FUNCTION, SUBUNIT, MUTAGENESIS OF ARG-63; ASN-66; ARG-163; GLY-203 AND HIS-235.

    Entry informationi

    Entry nameiCOE1_MOUSE
    AccessioniPrimary (citable) accession number: Q07802
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3