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Q07802

- COE1_MOUSE

UniProt

Q07802 - COE1_MOUSE

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Protein

Transcription factor COE1

Gene

Ebf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei163 – 1631Interaction with DNA
Sitei172 – 1721Interaction with DNA

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri151 – 17020C5-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. positive regulation of transcription, DNA-templated Source: MGI
  3. regulation of transcription, DNA-templated Source: MGI
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_252217. Transcriptional regulation of white adipocyte differentiation.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor COE1
Short name:
O/E-1
Short name:
OE-1
Alternative name(s):
Early B-cell factor
Gene namesi
Name:Ebf1
Synonyms:Coe1, Ebf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:95275. Ebf1.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi63 – 631R → A: Strongly reduced interaction with DNA. 1 Publication
Mutagenesisi66 – 661N → A: Reduced interaction with DNA. 1 Publication
Mutagenesisi163 – 1631R → A: Strongly reduced interaction with DNA. 1 Publication
Mutagenesisi203 – 2031G → E: Strongly reduced interaction with DNA. 1 Publication
Mutagenesisi235 – 2351H → A: Strongly reduced interaction with DNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591Transcription factor COE1PRO_0000107826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ07802.
PaxDbiQ07802.
PRIDEiQ07802.

PTM databases

PhosphoSiteiQ07802.

Expressioni

Tissue specificityi

Expressed at high levels in early B-cells, spleen, lymph node and adipose tissue, and at low levels in heart, brain, skeletal muscle and kidney. In adult expressed in olfactory epithelium, in spleen, and at a lesser extent in Purkinje cells of the cerebellum, heart, kidney, lung, thymus and testis. In embryo expressed in dorsal thalamus and epithalamus, at a lower level in mesencephalon and in the caudal rhombencephalon, in the postmitotic cells of developing retina, highly in developing spinal cord, dorsal root ganglia, trigeminal ganglia and in glossopharyngeal nerve ganglia, in developing inner ear.

Developmental stagei

First detected at 9.5 dpc.

Gene expression databases

BgeeiQ07802.
CleanExiMM_EBF1.
ExpressionAtlasiQ07802. baseline and differential.
GenevestigatoriQ07802.

Interactioni

Subunit structurei

Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes (By similarity). Forms either a homodimer or a heterodimer with a related family member.By similarity2 Publications

Protein-protein interaction databases

BioGridi199358. 1 interaction.

Structurei

Secondary structure

1
591
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 383Combined sources
Helixi39 – 446Combined sources
Beta strandi48 – 569Combined sources
Beta strandi60 – 634Combined sources
Beta strandi66 – 7611Combined sources
Beta strandi83 – 9311Combined sources
Helixi95 – 973Combined sources
Helixi99 – 1013Combined sources
Beta strandi108 – 1158Combined sources
Beta strandi121 – 13212Combined sources
Turni133 – 1353Combined sources
Helixi148 – 1503Combined sources
Beta strandi152 – 1554Combined sources
Helixi157 – 1604Combined sources
Helixi162 – 1654Combined sources
Helixi171 – 1755Combined sources
Beta strandi181 – 1833Combined sources
Turni184 – 1863Combined sources
Beta strandi187 – 1948Combined sources
Beta strandi201 – 2044Combined sources
Beta strandi211 – 2199Combined sources
Beta strandi221 – 2288Combined sources
Beta strandi232 – 2354Combined sources
Turni238 – 2414Combined sources
Turni249 – 2513Combined sources
Beta strandi263 – 2664Combined sources
Beta strandi271 – 2733Combined sources
Beta strandi279 – 2857Combined sources
Beta strandi292 – 2954Combined sources
Beta strandi298 – 3003Combined sources
Beta strandi302 – 3065Combined sources
Beta strandi309 – 3135Combined sources
Beta strandi321 – 3299Combined sources
Beta strandi332 – 3354Combined sources
Beta strandi340 – 3456Combined sources
Turni351 – 36111Combined sources
Helixi375 – 38915Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MLNX-ray2.40A/B/E24-241[»]
3MLOX-ray3.01A/B24-241[»]
3MLPX-ray2.80A/B/E/F24-421[»]
ProteinModelPortaliQ07802.
SMRiQ07802. Positions 35-413.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07802.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini262 – 34584IPT/TIGAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 664Interaction with DNA
Regioni197 – 2048Interaction with DNA
Regioni236 – 2394Interaction with DNA

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi462 – 55089Pro/Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the COE family.Curated
Contains 1 IPT/TIG domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri151 – 17020C5-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG259050.
HOGENOMiHOG000092311.
HOVERGENiHBG005108.
InParanoidiQ07802.
KOiK09103.
OrthoDBiEOG7J446V.
TreeFamiTF313391.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]
PANTHERiPTHR10747. PTHR10747. 1 hit.
PfamiPF01833. TIG. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS01345. COE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q07802-1) [UniParc]FASTAAdd to Basket

Also known as: 8

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA
60 70 80 90 100
RAHFEKQPPS NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN
110 120 130 140 150
SEKTNNGIHY RLQLLYSNGI RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM
160 170 180 190 200
CRVLLTHEIM CSRCCDKKSC GNRNETPSDP VIIDRFFLKF FLKCNQNCLK
210 220 230 240 250
NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS
260 270 280 290 300
EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV
310 320 330 340 350
WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP
360 370 380 390 400
TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII
410 420 430 440 450
LKRAADIAEA LYSVPRNHNQ LPALANTSVH AGMMGVNSFS GQLAVNVSEA
460 470 480 490 500
SQATNQGFTR NSSSVSPHGY VPSTTPQQTN YNSVTTSMNG YGSAAMSNLG
510 520 530 540 550
GSPTFLNGSA ANSPYAIVPS SPTMASSTSL PSNCSSSSGI FSFSPANMVS
560 570 580 590
AVKQKSAFAP VVRPQTSPPP TCTSTNGNSL QAISGMIVPP M
Length:591
Mass (Da):64,464
Last modified:November 1, 1996 - v1
Checksum:iE47797B6FC1E5071
GO
Isoform Short (identifier: Q07802-2) [UniParc]FASTAAdd to Basket

Also known as: O

The sequence of this isoform differs from the canonical sequence as follows:
     252-259: Missing.

Show »
Length:583
Mass (Da):63,579
Checksum:i81A00C02F4986723
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871T → F AA sequence (PubMed:8491377)Curated
Sequence conflicti89 – 891F → L AA sequence (PubMed:8491377)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei252 – 2598Missing in isoform Short. CuratedVSP_001112

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12147 mRNA. Translation: AAA37533.1.
CCDSiCCDS24566.1. [Q07802-1]
PIRiA40684.
RefSeqiNP_031923.1. NM_007897.3. [Q07802-1]
XP_006532219.1. XM_006532156.1. [Q07802-2]
UniGeneiMm.439662.
Mm.440346.

Genome annotation databases

EnsembliENSMUST00000081265; ENSMUSP00000080020; ENSMUSG00000057098. [Q07802-1]
GeneIDi13591.
KEGGimmu:13591.
UCSCiuc007ink.1. mouse. [Q07802-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12147 mRNA. Translation: AAA37533.1 .
CCDSi CCDS24566.1. [Q07802-1 ]
PIRi A40684.
RefSeqi NP_031923.1. NM_007897.3. [Q07802-1 ]
XP_006532219.1. XM_006532156.1. [Q07802-2 ]
UniGenei Mm.439662.
Mm.440346.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MLN X-ray 2.40 A/B/E 24-241 [» ]
3MLO X-ray 3.01 A/B 24-241 [» ]
3MLP X-ray 2.80 A/B/E/F 24-421 [» ]
ProteinModelPortali Q07802.
SMRi Q07802. Positions 35-413.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199358. 1 interaction.

PTM databases

PhosphoSitei Q07802.

Proteomic databases

MaxQBi Q07802.
PaxDbi Q07802.
PRIDEi Q07802.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081265 ; ENSMUSP00000080020 ; ENSMUSG00000057098 . [Q07802-1 ]
GeneIDi 13591.
KEGGi mmu:13591.
UCSCi uc007ink.1. mouse. [Q07802-1 ]

Organism-specific databases

CTDi 1879.
MGIi MGI:95275. Ebf1.

Phylogenomic databases

eggNOGi NOG259050.
HOGENOMi HOG000092311.
HOVERGENi HBG005108.
InParanoidi Q07802.
KOi K09103.
OrthoDBi EOG7J446V.
TreeFami TF313391.

Enzyme and pathway databases

Reactomei REACT_252217. Transcriptional regulation of white adipocyte differentiation.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSi Ebf1. mouse.
EvolutionaryTracei Q07802.
NextBioi 284202.
PROi Q07802.
SOURCEi Search...

Gene expression databases

Bgeei Q07802.
CleanExi MM_EBF1.
ExpressionAtlasi Q07802. baseline and differential.
Genevestigatori Q07802.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
InterProi IPR011598. bHLH_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view ]
PANTHERi PTHR10747. PTHR10747. 1 hit.
Pfami PF01833. TIG. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
SM00429. IPT. 1 hit.
[Graphical view ]
SUPFAMi SSF81296. SSF81296. 1 hit.
PROSITEi PS01345. COE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and functional characterization of early B-cell factor, a regulator of lymphocyte-specific gene expression."
    Hagman J., Belanger C., Travis A., Turck W., Grosschedl R.
    Genes Dev. 7:760-773(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 87-99; 122-129; 342-358 AND 382-390.
    Tissue: Lymphoid tissue.
  2. "Purification of early-B-cell factor and characterization of its DNA-binding specificity."
    Travis A., Hagman J., Hwang L., Grosschedl R.
    Mol. Cell. Biol. 13:3392-3400(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  3. "The characterization of the Olf-1/EBF-like HLH transcription factor family: implications in olfactory gene regulation and neuronal development."
    Wang S.S., Tsai R.Y.L., Reed R.R.
    J. Neurosci. 17:4149-4158(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, ALTERNATIVE SPLICING.
    Strain: CD-1.
    Tissue: Embryo.
  4. "Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins."
    Treiber N., Treiber T., Zocher G., Grosschedl R.
    Genes Dev. 24:2270-2275(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 24-241 IN COMPLEX WITH TARGET DNA, FUNCTION, SUBUNIT, MUTAGENESIS OF ARG-63; ASN-66; ARG-163; GLY-203 AND HIS-235.

Entry informationi

Entry nameiCOE1_MOUSE
AccessioniPrimary (citable) accession number: Q07802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3