Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q07802 (COE1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor COE1

Short name=O/E-1
Short name=OE-1
Alternative name(s):
Early B-cell factor
Gene names
Name:Ebf1
Synonyms:Coe1, Ebf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length591 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Ref.4

Subunit structure

Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes By similarity. Forms either a homodimer or a heterodimer with a related family member. Ref.3 Ref.4

Subcellular location

Nucleus Potential.

Tissue specificity

Expressed at high levels in early B-cells, spleen, lymph node and adipose tissue, and at low levels in heart, brain, skeletal muscle and kidney. In adult expressed in olfactory epithelium, in spleen, and at a lesser extent in Purkinje cells of the cerebellum, heart, kidney, lung, thymus and testis. In embryo expressed in dorsal thalamus and epithalamus, at a lower level in mesencephalon and in the caudal rhombencephalon, in the postmitotic cells of developing retina, highly in developing spinal cord, dorsal root ganglia, trigeminal ganglia and in glossopharyngeal nerve ganglia, in developing inner ear.

Developmental stage

First detected at 9.5 dpc.

Sequence similarities

Belongs to the COE family.

Contains 1 IPT/TIG domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q07802-1)

Also known as: 8;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q07802-2)

Also known as: O;

The sequence of this isoform differs from the canonical sequence as follows:
     252-259: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 591591Transcription factor COE1
PRO_0000107826

Regions

Domain262 – 34584IPT/TIG
Zinc finger151 – 17020C5-type Potential
Region63 – 664Interaction with DNA
Region197 – 2048Interaction with DNA
Region236 – 2394Interaction with DNA
Compositional bias462 – 55089Pro/Ser/Thr-rich

Sites

Site1631Interaction with DNA
Site1721Interaction with DNA

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence252 – 2598Missing in isoform Short.
VSP_001112

Experimental info

Mutagenesis631R → A: Strongly reduced interaction with DNA. Ref.4
Mutagenesis661N → A: Reduced interaction with DNA. Ref.4
Mutagenesis1631R → A: Strongly reduced interaction with DNA. Ref.4
Mutagenesis2031G → E: Strongly reduced interaction with DNA. Ref.4
Mutagenesis2351H → A: Strongly reduced interaction with DNA. Ref.4
Sequence conflict871T → F AA sequence Ref.1
Sequence conflict891F → L AA sequence Ref.1

Secondary structure

....................................................................... 591
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Long (8) [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E47797B6FC1E5071

FASTA59164,464
        10         20         30         40         50         60 
MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA RAHFEKQPPS 

        70         80         90        100        110        120 
NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN SEKTNNGIHY RLQLLYSNGI 

       130        140        150        160        170        180 
RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM CRVLLTHEIM CSRCCDKKSC GNRNETPSDP 

       190        200        210        220        230        240 
VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH 

       250        260        270        280        290        300 
GRRARRLDPS EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV 

       310        320        330        340        350        360 
WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP TIDYGFQRLQ 

       370        380        390        400        410        420 
KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII LKRAADIAEA LYSVPRNHNQ 

       430        440        450        460        470        480 
LPALANTSVH AGMMGVNSFS GQLAVNVSEA SQATNQGFTR NSSSVSPHGY VPSTTPQQTN 

       490        500        510        520        530        540 
YNSVTTSMNG YGSAAMSNLG GSPTFLNGSA ANSPYAIVPS SPTMASSTSL PSNCSSSSGI 

       550        560        570        580        590 
FSFSPANMVS AVKQKSAFAP VVRPQTSPPP TCTSTNGNSL QAISGMIVPP M 

« Hide

Isoform Short (O) [UniParc].

Checksum: 81A00C02F4986723
Show »

FASTA58363,579

References

[1]"Cloning and functional characterization of early B-cell factor, a regulator of lymphocyte-specific gene expression."
Hagman J., Belanger C., Travis A., Turck W., Grosschedl R.
Genes Dev. 7:760-773(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 87-99; 122-129; 342-358 AND 382-390.
Tissue: Lymphoid tissue.
[2]"Purification of early-B-cell factor and characterization of its DNA-binding specificity."
Travis A., Hagman J., Hwang L., Grosschedl R.
Mol. Cell. Biol. 13:3392-3400(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[3]"The characterization of the Olf-1/EBF-like HLH transcription factor family: implications in olfactory gene regulation and neuronal development."
Wang S.S., Tsai R.Y.L., Reed R.R.
J. Neurosci. 17:4149-4158(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, ALTERNATIVE SPLICING.
Strain: CD-1.
Tissue: Embryo.
[4]"Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins."
Treiber N., Treiber T., Zocher G., Grosschedl R.
Genes Dev. 24:2270-2275(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 24-241 IN COMPLEX WITH TARGET DNA, FUNCTION, SUBUNIT, MUTAGENESIS OF ARG-63; ASN-66; ARG-163; GLY-203 AND HIS-235.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12147 mRNA. Translation: AAA37533.1.
PIRA40684.
RefSeqNP_031923.1. NM_007897.2.
XP_006532219.1. XM_006532156.1.
UniGeneMm.439662.
Mm.440346.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3MLNX-ray2.40A/B/E24-241[»]
3MLOX-ray3.01A/B24-241[»]
3MLPX-ray2.80A/B/E/F24-421[»]
ProteinModelPortalQ07802.
SMRQ07802. Positions 35-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199358. 1 interaction.

PTM databases

PhosphoSiteQ07802.

Proteomic databases

PaxDbQ07802.
PRIDEQ07802.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081265; ENSMUSP00000080020; ENSMUSG00000057098. [Q07802-1]
GeneID13591.
KEGGmmu:13591.
UCSCuc007ink.1. mouse. [Q07802-1]

Organism-specific databases

CTD1879.
MGIMGI:95275. Ebf1.

Phylogenomic databases

eggNOGNOG259050.
HOGENOMHOG000092311.
HOVERGENHBG005108.
KOK09103.
OrthoDBEOG7J446V.
TreeFamTF313391.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Gene expression databases

ArrayExpressQ07802.
BgeeQ07802.
CleanExMM_EBF1.
GenevestigatorQ07802.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR011598. bHLH_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]
PANTHERPTHR10747. PTHR10747. 1 hit.
PfamPF01833. TIG. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
SM00429. IPT. 1 hit.
[Graphical view]
SUPFAMSSF81296. SSF81296. 1 hit.
PROSITEPS01345. COE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEBF1. mouse.
EvolutionaryTraceQ07802.
NextBio284202.
PROQ07802.
SOURCESearch...

Entry information

Entry nameCOE1_MOUSE
AccessionPrimary (citable) accession number: Q07802
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot