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Protein

Dihydrofolate reductase

Gene

DFR1

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (DFR1)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei80SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 29NADPBy similarity7
Nucleotide bindingi64 – 66NADPBy similarity3
Nucleotide bindingi86 – 88NADPBy similarity3
Nucleotide bindingi127 – 134NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:DFR1
Synonyms:DHFR
Ordered Locus Names:CNJ01940
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000002149 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiFungiDB:CNJ01940.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863751 – 229Dihydrofolate reductaseAdd BLAST229

Proteomic databases

PaxDbiQ07801.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi214684.XP_567366.1.

Structurei

3D structure databases

ProteinModelPortaliQ07801.
SMRiQ07801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 227DHFRPROSITE-ProRule annotationAdd BLAST217

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 42Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiKOG1324. Eukaryota.
COG0262. LUCA.
InParanoidiQ07801.
KOiK00287.
OrthoDBiEOG092C4LJX.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiView protein in InterPro
IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
PfamiView protein in Pfam
PF00186. DHFR_1. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiView protein in PROSITE
PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q07801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTTAKSSTP SITAVVAATA ENGIGLNGGL PWRLPGEMKY FARVTTGETP
60 70 80 90 100
SSDPSEQNVV IMGRKTWESI PSRFRPLKNR RNVVISGKGV DLGTAENSTV
110 120 130 140 150
YTDIPSALSA LRSTTESGHS PRIFLIGGAT LYTSSLLPSS VPSLNSSTST
160 170 180 190 200
SPLPFSRPLI DRILLTRILS PFECDAYLED FAAHTKPDGS KVWKKASIKE
210 220
FREWIGWDIE EQVEEKGVKY IFEMWVLNQ
Length:229
Mass (Da):25,169
Last modified:February 1, 1995 - v1
Checksum:i60D9E4FF66C5F198
GO

Sequence cautioni

The sequence AAW45849 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S58802 Genomic DNA. Translation: AAB26198.1.
AE017350 Genomic DNA. Translation: AAW45849.1. Different initiation.
PIRiA46049.
RefSeqiXP_567366.1. XM_567366.1.

Genome annotation databases

GeneIDi3254250.
KEGGicne:CNJ01940.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDYR_CRYNJ
AccessioniPrimary (citable) accession number: Q07801
Secondary accession number(s): Q5KAF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 15, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families