Reviewed, UniProtKB/Swiss-Prot Q07801 (DYR_CRYNE)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
    EC=1.5.1.3

Gene names

Name:	DFR1
Synonyms:	DHFR
Ordered Locus Names:	CNJ01940

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Subunit structure	Monomer.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Dihydrofolate reductase		PRO_0000186375
Regions
	Domain	11 – 227	217	DHFR

Sequences

	Sequence		Length	Mass (Da)
	Q07801-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 60D9E4FF66C5F198	FASTA	229	25,169
	10 20 30 40 50 60 MQTTAKSSTP SITAVVAATA ENGIGLNGGL PWRLPGEMKY FARVTTGETP SSDPSEQNVV 70 80 90 100 110 120 IMGRKTWESI PSRFRPLKNR RNVVISGKGV DLGTAENSTV YTDIPSALSA LRSTTESGHS 130 140 150 160 170 180 PRIFLIGGAT LYTSSLLPSS VPSLNSSTST SPLPFSRPLI DRILLTRILS PFECDAYLED 190 200 210 220 FAAHTKPDGS KVWKKASIKE FREWIGWDIE EQVEEKGVKY IFEMWVLNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and characterization of Cryptococcus neoformans dihydrofolate reductase." Sirawaraporn W., Cao M., Santi D.V., Edman J.C. J. Biol. Chem. 268:8888-8892(1993) [PubMed: 8473332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION.
[2]	"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JEC21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S58802 Genomic DNA. Translation: AAB26198.1. AE017350 Genomic DNA. Translation: AAW45849.1. Different initiation.
PIR	A46049.
RefSeq	XP_567366.1.
3D structure databases
HSSP	HSSP built from PDB template 1J3K based on UniProtKB P13922.
ModBase	Search...
Genome annotation databases
GeneID	3254250.
GenomeReviews	Gene locus CNJ01940 in contig AE017350_GR.
KEGG	cne:CNJ01940.
Phylogenomic databases
HOGENOM	Q07801.
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_CRYNE

Accession

Primary (citable) accession number: Q07801
Secondary accession number(s): Q5KAF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)