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Protein

Phosphatase PSR1

Gene

PSR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has phosphatase activity in vitro. Involved in the response to sodium and lithium ion stress (but not to potassium or sorbitol stress) by inducing transcription of the sodium pump ENA1/PMR2. Acts through a calcineurin-independent pathway and is functionally redundant with PSR2. Also involved in the general stress response; acts together with WHI2 to activate stress response element (STRE)-mediated gene expression, possibly through dephosphorylation of MSN2.1 Publication2 Publications

GO - Molecular functioni

  • phosphoprotein phosphatase activity Source: SGD

GO - Biological processi

  • cellular response to salt stress Source: SGD
  • protein dephosphorylation Source: GOC
  • response to heat Source: UniProtKB
  • response to salt stress Source: UniProtKB
  • response to stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciYEAST:G3O-32115-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatase PSR1 (EC:3.1.3.-)
Alternative name(s):
Plasma membrane sodium response protein 1
Gene namesi
Name:PSR1
Ordered Locus Names:YLL010C
ORF Names:L1341
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL010C.
SGDiS000003933. PSR1.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: No effect on membrane association. 1 Publication
Mutagenesisi9 – 102CC → GG: Impairs membrane association. 1 Publication
Mutagenesisi263 – 2631D → E: Low residual phosphatase activity. Loss of stress response functions. 1 Publication
Mutagenesisi265 – 2651D → E: Low residual phosphatase activity. Loss of stress response functions. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Phosphatase PSR1PRO_0000212576Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi9 – 91S-palmitoyl cysteine1 PublicationSequence analysis
Lipidationi10 – 101S-palmitoyl cysteine1 PublicationSequence analysis
Modified residuei110 – 1101PhosphoserineCombined sources

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ07800.

PTM databases

iPTMnetiQ07800.
SwissPalmiQ07800.

Interactioni

Subunit structurei

Interacts with WHI2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
WHI2P126113EBI-31129,EBI-20530

Protein-protein interaction databases

BioGridi31241. 35 interactions.
DIPiDIP-4182N.
IntActiQ07800. 1 interaction.
MINTiMINT-513454.

Structurei

3D structure databases

ProteinModelPortaliQ07800.
SMRiQ07800. Positions 246-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini253 – 411159FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi152 – 18938Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000017194.
HOGENOMiHOG000211190.
InParanoidiQ07800.
KOiK15731.
OrthoDBiEOG7VDZ0W.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07800-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFISSILCC SSETTQSNSN SAYRQQQSSS LNKNRSVKHS NTKSRTRGVH
60 70 80 90 100
QTNSPPSKTN SAATFSSTER STGKSGISTN DNEKKKPSSP TAAVTATTTN
110 120 130 140 150
NMTKVEKRIS KDDLYEEKYE VDEDEEIDDE DNRRSRGIVQ EKGDAVKDTS
160 170 180 190 200
RQKKQQQQQQ QQSQPQPQPQ SQSQSQSQSQ SQQRGPTVQV SSDHLIQDMN
210 220 230 240 250
LSRVSSSSQA SETSNDADDE DDEDEEYIDL TLLQQGQYHA PGYNTLLPPQ
260 270 280 290 300
DESTKGKKCL ILDLDETLVH SSFKYLRSAD FVLSVEIDDQ VHNVYVIKRP
310 320 330 340 350
GVEEFLERVG KLFEVVVFTA SVSRYGDPLL DILDTDKVIH HRLFREACYN
360 370 380 390 400
YEGNYIKNLS QIGRPLSDII ILDNSPASYI FHPQHAIPIS SWFSDTHDNE
410 420
LLDIIPLLED LSVKTSLDVG KILDVTI
Length:427
Mass (Da):47,931
Last modified:November 1, 1996 - v1
Checksum:i402F5C395132A080
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91488 Genomic DNA. Translation: CAA62782.1.
Z73115 Genomic DNA. Translation: CAA97454.1.
BK006945 Genomic DNA. Translation: DAA09308.1.
PIRiS64752.
RefSeqiNP_013091.1. NM_001181830.1.

Genome annotation databases

EnsemblFungiiYLL010C; YLL010C; YLL010C.
GeneIDi850650.
KEGGisce:YLL010C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91488 Genomic DNA. Translation: CAA62782.1.
Z73115 Genomic DNA. Translation: CAA97454.1.
BK006945 Genomic DNA. Translation: DAA09308.1.
PIRiS64752.
RefSeqiNP_013091.1. NM_001181830.1.

3D structure databases

ProteinModelPortaliQ07800.
SMRiQ07800. Positions 246-412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31241. 35 interactions.
DIPiDIP-4182N.
IntActiQ07800. 1 interaction.
MINTiMINT-513454.

PTM databases

iPTMnetiQ07800.
SwissPalmiQ07800.

Proteomic databases

MaxQBiQ07800.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL010C; YLL010C; YLL010C.
GeneIDi850650.
KEGGisce:YLL010C.

Organism-specific databases

EuPathDBiFungiDB:YLL010C.
SGDiS000003933. PSR1.

Phylogenomic databases

GeneTreeiENSGT00390000017194.
HOGENOMiHOG000211190.
InParanoidiQ07800.
KOiK15731.
OrthoDBiEOG7VDZ0W.

Enzyme and pathway databases

BioCyciYEAST:G3O-32115-MONOMER.

Miscellaneous databases

PROiQ07800.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the CEN12 region of Saccharomyces cerevisiae on a 43.7 kb fragment of chromosome XII including an open reading frame homologous to the human cystic fibrosis transmembrane conductance regulator protein CFTR."
    Miosga T., Zimmermann F.K.
    Yeast 12:693-708(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90840 / EAY235 / FY23.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Psr1p/Psr2p, two plasma membrane phosphatases with an essential DXDX(T/V) motif required for sodium stress response in yeast."
    Siniossoglou S., Hurt E.C., Pelham H.R.B.
    J. Biol. Chem. 275:19352-19360(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PALMITOYLATION AT CYS-9 AND CYS-10, PHOSPHORYLATION, MUTAGENESIS OF GLY-2; 9-CYS-CYS-10; ASP-263 AND ASP-265.
  5. "Yeast Whi2 and Psr1-phosphatase form a complex and regulate STRE-mediated gene expression."
    Kaida D., Yashiroda H., Toh-e A., Kikuchi Y.
    Genes Cells 7:543-552(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH WHI2.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSR1_YEAST
AccessioniPrimary (citable) accession number: Q07800
Secondary accession number(s): D6VXZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5620 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.