Q07792 (ESTE_VIBMI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Arylesterase EC=3.1.1.2 Alternative name(s): Aryl-ester hydrolase
Organism	Vibrio mimicus
Taxonomic identifier	674 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Favors the hydrolysis of several arylesters.
Catalytic activity	A phenyl acetate + H2O = a phenol + acetate.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the 'GDSL' lipolytic enzyme family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	arylesterase activity Inferred from electronic annotation. Source: EC lipase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.1
	Chain	20 – 200	181	Arylesterase		PRO_0000017847
Sites
	Active site	29	1	Nucleophile By similarity
	Active site	176	1	By similarity
	Active site	179	1	By similarity
Experimental info
	Mutagenesis	29	1	S → A or C: Loss of activity.
	Mutagenesis	31	1	S → A: No loss of activity.

Sequences

	Sequence		Length	Mass (Da)
	Q07792 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7EC1885B1112D432	FASTA	200	22,251
	10 20 30 40 50 60 MIRLLSLVLF FCLSAASQAS EKLLVLGDSL SAGYQMPIEK SWPSLLPDAL LEHGQDVTVI 70 80 90 100 110 120 NGSISGDTTG NGLARLPQLL DQHTPDLVLI ELGANDGLRG FPPKVITSNL SKMISLIKDS 130 140 150 160 170 180 GANVVMMQIR VPPNYGKRYS DMFYDIYPKL AEHQQVQLMP FFLEHVITKP EWMMDDGLHP 190 200 KPEAQPWIAE FVAQELVKHL

References

[1]

"Nucleotide sequence of a novel arylesterase gene from Vibro mimicus and characterization of the enzyme expressed in Escherichia coli." Shaw J.-F., Chang R.-C., Chuang K.-H., Yen Y.-T., Wang Y.-J., Wang F.-G. Biochem. J. 298:675-680(1994) [PubMed: 8141782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20-29, MUTAGENESIS. Strain: NTOU 66.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71116 Genomic DNA. Translation: CAA50433.1.
PIR	S32044. S43387.
3D structure databases
ProteinModelPortal	Q07792.
SMR	Q07792. Positions 20-200.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013830. Esterase_SGNH_hydro-type. IPR013831. Esterase_SGNH_hydro-type_subgr. IPR001087. Lipase_GDSL. IPR008265. Lipase_GDSL_AS. [Graphical view]
Gene3D	G3DSA:3.40.50.1110. Esterase_SGNH_hydro-type_subgr. 1 hit.
Pfam	PF00657. Lipase_GDSL. 1 hit. [Graphical view]
SUPFAM	SSF52266. Esterase_SGNH_hydro-type. 1 hit.
PROSITE	PS01098. LIPASE_GDSL_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ESTE_VIBMI

Accession

Primary (citable) accession number: Q07792

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 31, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program