Reviewed, UniProtKB/Swiss-Prot Q07785 (CDC2H_PLAFK)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2 homolog
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    PfPK5

Gene names

Name:	CRK2
Synonyms:	PK5

Organism

Plasmodium falciparum (isolate K1 / Thailand)

Taxonomic identifier

5839 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Laverania)

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-158 activates it By similarity.
Subunit structure	Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin By similarity.
Subcellular location	Cytoplasm.
Developmental stage	Present in approximately constant amounts throughout the intra-erythrocytic asexual reproductive stage of the life cycle.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 288

288

Cell division control protein 2 homolog

PRO_0000085742

Regions

Domain

4 – 284

281

Protein kinase

Nucleotide binding

10 – 18

9

ATP By similarity

Sites

Active site

125

1

Proton acceptor By similarity

Binding site

32

1

ATP By similarity

Amino acid modifications

Modified residue

14

1

Phosphothreonine By similarity

Modified residue

15

1

Phosphotyrosine By similarity

Modified residue

158

1

Phosphothreonine; by CAK By similarity

Secondary structure

1

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288

Helix Strand Turn

Details...

Beta strand

3 – 13

11

Beta strand

16 – 23

8

Beta strand

28 – 34

7

Helix

39 – 41

3

Helix

45 – 52

8

Helix

53 – 56

4

Beta strand

65 – 70

6

Beta strand

75 – 80

6

Beta strand

83 – 85

3

Helix

86 – 91

6

Helix

99 – 118

20

Helix

128 – 130

3

Beta strand

131 – 133

3

Beta strand

139 – 141

3

Helix

146 – 150

5

Helix

169 – 172

4

Helix

181 – 196

16

Helix

206 – 217

12

Turn

222 – 224

3

Helix

228 – 230

3

Helix

246 – 248

3

Helix

255 – 264

10

Turn

269 – 271

3

Helix

275 – 279

5

Helix

282 – 285

4

Sequences

	Sequence		Length	Mass (Da)
	Q07785-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: BF8C2935F872F6F0	FASTA	288	32,996
	10 20 30 40 50 60 MEKYHGLEKI GEGTYGVVYK AQNNYGETFA LKKIRLEKED EGIPSTTIRE ISILKELKHS 70 80 90 100 110 120 NIVKLYDVIH TKKRLVLVFE HLDQDLKKLL DVCEGGLESV TAKSFLLQLL NGIAYCHDRR 130 140 150 160 170 180 VLHRDLKPQN LLINREGELK IADFGLARAF GIPVRKYTHE VVTLWYRAPD VLMGSKKYST 190 200 210 220 230 240 TIDIWSVGCI FAEMVNGTPL FPGVSEADQL MRIFRILGTP NSKNWPNVTE LPKYDPNFTV 250 260 270 280 YEPLPWESFL KGLDESGIDL LSKMLKLDPN QRITAKQALE HAYFKENN

References

[1]	"Isolation and expression of a gene specifying a cdc2-like protein kinase from the human malaria parasite Plasmodium falciparum." Ross-Macdonald P.B., Graeser R., Kappes B., Franklin R., Williamson D.H. Eur. J. Biochem. 220:693-701(1994) [PubMed: 8143724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X61921 Genomic DNA. Translation: CAA43923.1.

PIR

S42566.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LCH	model	-	A	1-288	[»]
1OB3	X-ray	1.90	A/B	1-288	[»]
1V0B	X-ray	2.20	A/B	1-288	[»]
1V0O	X-ray	1.90	A/B	1-288	[»]
1V0P	X-ray	2.00	A/B	1-288	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

2.7.11.22. 1455.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
IPR002290. Ser/Thr_prot_kinase_dom.
[Graphical view]

Pfam

PF00069. Pkinase. 1 hit.
[Graphical view]

SMART

SM00220. S_TKc. 1 hit.
[Graphical view]

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CDC2H_PLAFK

Accession

Primary (citable) accession number: Q07785

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 24, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)