Reviewed, UniProtKB/Swiss-Prot Q07737 (CHVG_AGRT5)

Names and origin

Protein names

Recommended name:
    Sensor protein chvG
    EC=2.7.13.3

Gene names

Name:	chvG
Ordered Locus Names:	Atu0033
ORF Names:	AGR_C_52

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of a two-component regulatory system chvG/chvI. Activates chvI by phosphorylation Potential.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Probable.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.
Sequence caution	The sequence AAA03552.1 differs from that shown. Reason: Frameshift at positions 18, 152, 183, 216, 234 and 567.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Sensor protein chvG		PRO_0000074720
Regions
	Topological domain	1 – 47	47	Cytoplasmic Potential
	Transmembrane	48 – 68	21	Potential
	Topological domain	69 – 283	215	Periplasmic Potential
	Transmembrane	284 – 304	21	Potential
	Topological domain	305 – 596	292	Cytoplasmic Potential
	Domain	301 – 356	56	HAMP
	Domain	364 – 592	229	Histidine kinase
Amino acid modifications
	Modified residue	367	1	Phosphohistidine By similarity
Experimental info
	Sequence conflict	94	1	A → R in AAA03552. Ref.1
	Sequence conflict	236	1	R → P Ref.1
	Sequence conflict	314 – 318	5	AIRVR → RSGA in AAA03552. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q07737-1 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: 8931990E49736C4B	FASTA	596	65,722
	10 20 30 40 50 60 MLKKTPETVS DSDDAEERGS ERRHRIHPLT IIRRIFGNAV FSSLTRRILF FNVAATVVLV 70 80 90 100 110 120 GGILYLNQFR EGLIDARVES LLTQGEIIAG AVSASASVDT NSITINPEKL LELQAGQSIT 130 140 150 160 170 180 PAPNDEDLSF PINPERVAPV LRRLISPTRT RARLFDADAN LLLDSRHLYS RGQVLRFDLP 190 200 210 220 230 240 PVTPETQTWG DWFTSMFNRM LQPSSLPQYK EAPGGDGSIY PEVMNALTGV RGAVVRVTEK 250 260 270 280 290 300 GELIVSVAVP VQRFRAVLGV LLLSTQAGDI DKIVHAERLA IMRVFGIATL VNIVLSLLLS 310 320 330 340 350 360 STIATPLRRL SAAAIRVRRG ARTREEIPDF SARQDEIGNL SIALREMTTA LYDRIDAIES 370 380 390 400 410 420 FAADVSHELK NPLTSLRSAV ETLPRAKTEE SKQRLTEIIF HDVRRLDRLI SDISDASRLD 430 440 450 460 470 480 AELARADASP LDLDVLMKGL VDISRQISTK KKSVAIDYVA DRKAGAKTSF VVNGHDLRIG 490 500 510 520 530 540 QIVTNLIENA RSFVSEESGR ITVRLSRHKD RCIVQVEDNG PGIQAEDIDR IFERFYTDRP 550 560 570 580 590 ASEGFGQNSG LGLSISRQIA EAHGGSLRAE NVVDKYGVIS GARFTLSLPA AETHER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens." Charles T.C., Nester E.W. J. Bacteriol. 175:6614-6625(1993) [PubMed: 8407839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L18860 Unassigned DNA. Translation: AAA03552.1. Frameshift. AE007869 Genomic DNA. Translation: AAK85857.1.
PIR	A49902. AB2581. H97362.
RefSeq	NP_353072.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q07737.
Genome annotation databases
GeneID	1132071.
GenomeReviews	Gene locus Atu0033 in contig AE007869_GR.
KEGG	atc:AGR_C_52. atu:Atu0033.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q07737.
OMA	AENRPGP.
Enzyme and pathway databases
BRENDA	2.7.13.3. 2199.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHVG_AGRT5

Accession

Primary (citable) accession number: Q07737

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	March 27, 2002
Last modified:	November 3, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)