Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glial cell line-derived neurotrophic factor

Gene

Gdnf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. May also modulate local neuronal effects in distal regions of the motor neuron.1 Publication

GO - Molecular functioni

  • glial cell-derived neurotrophic factor receptor binding Source: CAFA
  • growth factor activity Source: RGD
  • protein homodimerization activity Source: CAFA
  • receptor ligand activity Source: CAFA
  • receptor tyrosine kinase binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionGrowth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Glial cell line-derived neurotrophic factor
Alternative name(s):
Astrocyte-derived trophic factor
Short name:
ATF
Gene namesi
Name:Gdnf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2677 Gdnf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000003400820 – 75Add BLAST56
ChainiPRO_000003400978 – 211Glial cell line-derived neurotrophic factorAdd BLAST134

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi118 ↔ 1791 Publication
Glycosylationi126N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi145 ↔ 2081 Publication
Disulfide bondi149 ↔ 2101 Publication
Glycosylationi162N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi178Interchain1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ07731

Expressioni

Tissue specificityi

Expressed in both the central nervous system (CNS) and in non-CNS tissues, including the kidney, lung, bone, heart, liver, spleen, sciatic nerve and blood. In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Localizes at the proximal ligature of the hypoglossal nerve.3 Publications

Inductioni

By melatonin.1 Publication

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

GO - Molecular functioni

  • glial cell-derived neurotrophic factor receptor binding Source: CAFA
  • growth factor activity Source: RGD
  • protein homodimerization activity Source: CAFA
  • receptor ligand activity Source: CAFA
  • receptor tyrosine kinase binding Source: CAFA

Protein-protein interaction databases

BioGridi247487, 1 interactor
STRINGi10116.ENSRNOP00000044012

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi118 – 126Combined sources9
Helixi127 – 130Combined sources4
Beta strandi139 – 147Combined sources9
Helixi155 – 166Combined sources12
Beta strandi178 – 184Combined sources7
Beta strandi188 – 191Combined sources4
Beta strandi197 – 200Combined sources4
Beta strandi204 – 211Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AGQX-ray1.90A/B/C/D78-211[»]
DisProtiDP00029
ProteinModelPortaliQ07731
SMRiQ07731
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07731

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family. GDNF subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGT5 Eukaryota
ENOG4111I3D LUCA
HOVERGENiHBG106680
InParanoidiQ07731
KOiK05452
PhylomeDBiQ07731

Family and domain databases

Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR029034 Cystine-knot_cytokine
IPR016649 GDNF
IPR001839 TGF-b_C
PfamiView protein in Pfam
PF00019 TGF_beta, 1 hit
PIRSFiPIRSF016238 GDNF, 1 hit
SUPFAMiSSF57501 SSF57501, 1 hit
PROSITEiView protein in PROSITE
PS51362 TGF_BETA_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q07731-1) [UniParc]FASTAAdd to basket
Also known as: GDNF633

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLWDVVAVC LVLLHTASAF PLPAGKRLLE APAEDHSLGH RRVPFALTSD
60 70 80 90 100
SNMPEDYPDQ FDDVMDFIQA TIKRLKRSPD KQAAALPRRE RNRQAAAASP
110 120 130 140 150
ENSRGKGRRG QRGKNRGCVL TAIHLNVTDL GLGYETKEEL IFRYCSGSCE
160 170 180 190 200
AAETMYDKIL KNLSRSRRLT SDKVGQACCR PVAFDDDLSF LDDSLVYHIL
210
RKHSAKRCGC I
Length:211
Mass (Da):23,619
Last modified:February 1, 1995 - v1
Checksum:iAE06C646682895A5
GO
Isoform 2 (identifier: Q07731-2) [UniParc]FASTAAdd to basket
Also known as: ATF-1, GDNF555, sGDNF, Smaller GDNF

The sequence of this isoform differs from the canonical sequence as follows:
     25-51: GKRLLEAPAEDHSLGHRRVPFALTSDS → A

Show »
Length:185
Mass (Da):20,748
Checksum:iDAF95B585293C0CE
GO
Isoform 3 (identifier: Q07731-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.

Show »
Length:159
Mass (Da):17,985
Checksum:i54BE42BC83203895
GO
Isoform 4 (identifier: Q07731-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MKLWDVVAVCLVLLHTASAF → MGFGPLGVNVQLGVYGDRIR

Show »
Length:211
Mass (Da):23,551
Checksum:i33D2F285680D2FED
GO

Sequence cautioni

The sequence CAB64357 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB64358 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77R → S in AAB33891 (PubMed:7895811).Curated1
Sequence conflicti77R → S in AAB33892 (PubMed:7895811).Curated1
Sequence conflicti90E → K in AAB33891 (PubMed:7895811).Curated1
Sequence conflicti90E → K in AAB33892 (PubMed:7895811).Curated1
Sequence conflicti94Q → L in AAF23767 (PubMed:10828539).Curated1
Sequence conflicti101E → D AA sequence (PubMed:8493557).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0263651 – 52Missing in isoform 3. 1 PublicationAdd BLAST52
Alternative sequenceiVSP_0263661 – 20MKLWD…TASAF → MGFGPLGVNVQLGVYGDRIR in isoform 4. CuratedAdd BLAST20
Alternative sequenceiVSP_00642225 – 51GKRLL…LTSDS → A in isoform 2. 3 PublicationsAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15305 mRNA Translation: AAA67909.1
S75583 mRNA Translation: AAB33891.1
S75585 mRNA Translation: AAB33892.1
AF205713 mRNA Translation: AAF23767.1
AF205714 mRNA Translation: AAF23768.1
AF205715 mRNA Translation: AAF23769.1
X92495 mRNA Translation: CAA63237.1
AJ011432 Genomic DNA Translation: CAB64357.1 Sequence problems.
AJ011432 Genomic DNA Translation: CAB64358.1 Sequence problems.
AF497634 mRNA Translation: AAM18096.1
PIRiA37499
RefSeqiNP_062012.1, NM_019139.1 [Q07731-1]
UniGeneiRn.53970

Genome annotation databases

GeneIDi25453
KEGGirno:25453

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGDNF_RAT
AccessioniPrimary (citable) accession number: Q07731
Secondary accession number(s): Q63214
, Q64062, Q64063, Q8R485, Q9QX94, Q9QX95, Q9QXJ7, Q9QXJ8, Q9QXJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health