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Reviewed, UniProtKB/Swiss-Prot Q07729 (GUAD_YEAST)

Last modified June 16, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable guanine deaminase
      Short name=Guanase
      Short name=Guanine aminase
    EC=3.5.4.3
Alternative name(s):
    Guanine aminohydrolase
      Short name=GAH
Gene names
Name: GUD1
Ordered Locus Names: YDL238C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia By similarity.

Catalytic activity

Guanine + H2O = xanthine + NH3.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.

Miscellaneous

Present with 279 molecules/cell in log phase SD medium. Ref.2

Sequence similarities

Belongs to the ATZ/TRZ family.

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Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from direct assay. Source: SGD

   Molecular functionguanine deaminase activity

Inferred from direct assay. Source: SGD

protein binding

Inferred from physical interaction. Source: IntAct

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

P381501EBI-7985,EBI-21180

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Probable guanine deaminase
PRO_0000122302

Sites

Metal binding1001Zinc; via tele nitrogen By similarity
Metal binding1021Zinc; via tele nitrogen By similarity
Metal binding2581Zinc; via tele nitrogen By similarity
Metal binding3481Zinc By similarity
Binding site1051Substrate By similarity
Binding site2311Substrate By similarity
Binding site2611Substrate By similarity
Binding site2971Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q07729-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 60D21F40CA23DF81

FASTA48955,204
        10         20         30         40         50         60 
MTKSDLLFDK FNDKHGKFLV FFGTFVDTPK LGELRIREKT SVGVLNGIIR FVNRNSLDPV 

        70         80         90        100        110        120 
KDCLDHDSSL SPEDVTVVDI IGKDKTRNNS FYFPGFVDTH NHVSQYPNVG VFGNSTLLDW 

       130        140        150        160        170        180 
LEKYTFPIEA ALANENIARE VYNKVISKTL SHGTTTVAYY NTIDLKSTKL LAQLSSLLGQ 

       190        200        210        220        230        240 
RVLVGKVCMD TNGPEYYIED TKTSFESTVK VVKYIRETIC DPLVNPIVTP RFAPSCSREL 

       250        260        270        280        290        300 
MQQLSKLVKD ENIHVQTHLS ENKEEIQWVQ DLFPECESYT DVYDKYGLLT EKTVLAHCIH 

       310        320        330        340        350        360 
LTDAEARVIK QRRCGISHCP ISNSSLTSGE CRVRWLLDQG IKVGLGTDVS AGHSCSILTT 

       370        380        390        400        410        420 
GRQAFAVSRH LAMRETDHAK LSVSECLFLA TMGGAQVLRM DETLGTFDVG KQFDAQMIDT 

       430        440        450        460        470        480 
NAPGSNVDMF HWQLKEKDQM QEQEQEQGQD PYKNPPLLTN EDIIAKWFFN GDDRNTTKVW 


VAGQQVYQI

« Hide

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References

[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

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Cross-references

Sequence databases

Z74286 Genomic DNA. Translation: CAA98818.1.
PIRS67802.
RefSeqNP_010043.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:5170N.
IntActQ07729. 1 interaction.

Proteomic databases

PeptideAtlasQ07729.

Genome annotation databases

EnsemblYDL238C. Saccharomyces cerevisiae. [Contig view]
GeneID851360.
GenomeReviewsGene locus YDL238C in contig Z71256_GR.
KEGGsce:YDL238C.
NMPDRfig|4932.3.peg.776.

Organism-specific databases

CYGDYDL238c.
SGDS000002397. GUD1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMQ07729.
OMAQ07729. EDIIAKW.

Enzyme and pathway databases

BRENDA3.5.4.3. 250.

Gene expression databases

ArrayExpressQ07729.
GermOnlineYDL238C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR006680. Amidohydro_1.
IPR014311. Guanine_deaminase.
[Graphical view]
PANTHERPTHR11271:SF6. Guanine_deaminase. 1 hit.
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR02967. guan_deamin. 1 hit.
ProtoNetSearch...

Other Resources

NextBio968462.

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Entry information

Entry nameGUAD_YEAST
AccessionPrimary (citable) accession number: Q07729
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents