1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4

Preferred order of sections

Names and origin

Protein names

Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
EC=3.1.4.11

Alternative name(s):
PCL-C1
Phosphoinositide phospholipase C-beta-4
Phospholipase C-beta-4
Short name=PLC-beta-4

Gene names

Name:

PLCB4

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	1023 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This form has a role in retina signal transduction.
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H₂O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Calcium.
Tissue specificity	Preferentially expressed in the retina.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Coding sequence diversity	Alternative splicing
Ligand	Calcium
Molecular function	Hydrolase Transducer
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phosphatidylinositol phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist.

Isoform 1B (identifier: Q07722-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1A (identifier: Q07722-2) The sequence of this isoform differs from the canonical sequence as follows: 372-383: Missing.

Isoform 2A (identifier: Q07722-3) The sequence of this isoform differs from the canonical sequence as follows: 1-104: Missing. 372-383: Missing.

Isoform 2B (identifier: Q07722-4) The sequence of this isoform differs from the canonical sequence as follows: 1-104: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

‹1 – 1023

›1023

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4

PRO_0000088494

Regions

Domain

149 – 299

151

PI-PLC X-box

Domain

413 – 529

117

PI-PLC Y-box

Domain

536 – 634

99

C2

Sites

Active site

164

1

By similarity

Active site

211

1

By similarity

Amino acid modifications

Modified residue

734

1

Phosphothreonine By similarity

Natural variations

Alternative sequence

‹1 – 104

›104

Missing in isoform 2A and isoform 2B.

VSP_004719

Alternative sequence

372 – 383

12

Missing in isoform 1A and isoform 2A.

VSP_004720

Experimental info

Sequence conflict

24

1

E → D AA sequence Ref.2

Sequence conflict

32

1

N → D AA sequence Ref.2

Sequence conflict

36

1

E → L AA sequence Ref.2

Sequence conflict

328

1

M → I AA sequence Ref.2

Non-terminal residue

1

Sequences

Sequence		Length	Mass (Da)
Isoform 1B [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 416D700C2095748C Show »	FASTA	1,023	117,110
10 20 30 40 50 60 PVRSITRTFA SGKTEKVIFQ ALKELGLPSG KNDEIEPAAF TYEKFYELTQ KICPRTDIED 70 80 90 100 110 120 LFKKINGDKT DYLTVDQLVS FLNEHQRDPR LNEILFPFYD AKRAMQIIEM YEPDEDLKKQ 130 140 150 160 170 180 GLISSDGFCR YLMSDENAPV FLDRLELYQE MDHPLAHYFI SSSHNTYLTG RQFGGKSSVE 190 200 210 220 230 240 MYRQVLLAGC RCVELDCWDG KGEDQEPIIT HGKAMCTDIL FKDVIQAIKE TAFVTSEYPV 250 260 270 280 290 300 ILSFENHCSK YQQYKMSKYC EDLFGDLLLK QALESHPLEP GRPLPSPNDL KRKILIKKQT 310 320 330 340 350 360 TETEVEKKQL EALKSMMEAG ESAAPVNMLE DDNEEEIESA EQEEEAHPEY KYGNELSADD 370 380 390 400 410 420 LGHKEAIANS VKKASDDLEH ENSKKGLVTV EDEQAWMASY KYVGATTNIH PYLSTMINYA 430 440 450 460 470 480 QPVKFQGFHV AEERNIHYNM SSFNESVGLG YLKTHAIEFV NYNKRQMSRI YPKGGRVDSS 490 500 510 520 530 540 NYMPQIFWNS GCQMVSLNYQ TPDLAMQLNQ GKFEYNGSCG YLLKPDFMRR PDRTFDPFSE 550 560 570 580 590 600 TPVDGVIAAT CSVQVISGQF LSDKKIGTYV EVDMYGLPTD TIRKEFRTRM VMNNGLNPVY 610 620 630 640 650 660 NEESFVFRKV ILPDLAVLRI AVYDDNNKLI GQRILPLDGL QAGYRHISLR NEGNKPLSLP 670 680 690 700 710 720 TIFCNIVLKT YVPDGFGDIV DALSDPKKFL SITEKRADQM RAMGIETSDI ADVPSDTSKN 730 740 750 760 770 780 DKKGKANTAK ANVTPQSSSE LRPTTTAALG AGLEAKKGIE LIPQVRIEDL KQMKAYLKHL 790 800 810 820 830 840 KKQQKELSSL KKKHAKEHST MQKLHCTQVD KIVAQYDKEK LTHEKILEKA MKKKGGSNCL 850 860 870 880 890 900 EMKKETEIKI QTLTSDHKSK VKEIVAQHTK EWSDMINTHS AEEQEIRDLL LSQQCELLRK 910 920 930 940 950 960 LLISAHEQQT QQLKLSHDRE SKEMRAHQAK ISMENSKAIS QDKSIKNKAE RERRVRELNS 970 980 990 1000 1010 1020 SNTKKFLEER KRLAMKQSKE MDQLKKVQLE HLEFVEKQNE QAKEMQQMVK LEAEMDRRPA TVV « Hide
Isoform 1A [UniParc]. Checksum: FCC5C77F5A16FC06 Show »	FASTA	1,011	115,755
10 20 30 40 50 60 PVRSITRTFA SGKTEKVIFQ ALKELGLPSG KNDEIEPAAF TYEKFYELTQ KICPRTDIED 70 80 90 100 110 120 LFKKINGDKT DYLTVDQLVS FLNEHQRDPR LNEILFPFYD AKRAMQIIEM YEPDEDLKKQ 130 140 150 160 170 180 GLISSDGFCR YLMSDENAPV FLDRLELYQE MDHPLAHYFI SSSHNTYLTG RQFGGKSSVE 190 200 210 220 230 240 MYRQVLLAGC RCVELDCWDG KGEDQEPIIT HGKAMCTDIL FKDVIQAIKE TAFVTSEYPV 250 260 270 280 290 300 ILSFENHCSK YQQYKMSKYC EDLFGDLLLK QALESHPLEP GRPLPSPNDL KRKILIKKQT 310 320 330 340 350 360 TETEVEKKQL EALKSMMEAG ESAAPVNMLE DDNEEEIESA EQEEEAHPEY KYGNELSADD 370 380 390 400 410 420 LGHKEAIANS VKKGLVTVED EQAWMASYKY VGATTNIHPY LSTMINYAQP VKFQGFHVAE 430 440 450 460 470 480 ERNIHYNMSS FNESVGLGYL KTHAIEFVNY NKRQMSRIYP KGGRVDSSNY MPQIFWNSGC 490 500 510 520 530 540 QMVSLNYQTP DLAMQLNQGK FEYNGSCGYL LKPDFMRRPD RTFDPFSETP VDGVIAATCS 550 560 570 580 590 600 VQVISGQFLS DKKIGTYVEV DMYGLPTDTI RKEFRTRMVM NNGLNPVYNE ESFVFRKVIL 610 620 630 640 650 660 PDLAVLRIAV YDDNNKLIGQ RILPLDGLQA GYRHISLRNE GNKPLSLPTI FCNIVLKTYV 670 680 690 700 710 720 PDGFGDIVDA LSDPKKFLSI TEKRADQMRA MGIETSDIAD VPSDTSKNDK KGKANTAKAN 730 740 750 760 770 780 VTPQSSSELR PTTTAALGAG LEAKKGIELI PQVRIEDLKQ MKAYLKHLKK QQKELSSLKK 790 800 810 820 830 840 KHAKEHSTMQ KLHCTQVDKI VAQYDKEKLT HEKILEKAMK KKGGSNCLEM KKETEIKIQT 850 860 870 880 890 900 LTSDHKSKVK EIVAQHTKEW SDMINTHSAE EQEIRDLLLS QQCELLRKLL ISAHEQQTQQ 910 920 930 940 950 960 LKLSHDRESK EMRAHQAKIS MENSKAISQD KSIKNKAERE RRVRELNSSN TKKFLEERKR 970 980 990 1000 1010 LAMKQSKEMD QLKKVQLEHL EFVEKQNEQA KEMQQMVKLE AEMDRRPATV V « Hide
Isoform 2A [UniParc]. Checksum: 60FF44549D54DB2E Show »	FASTA	907	103,699
10 20 30 40 50 60 MQIIEMYEPD EDLKKQGLIS SDGFCRYLMS DENAPVFLDR LELYQEMDHP LAHYFISSSH 70 80 90 100 110 120 NTYLTGRQFG GKSSVEMYRQ VLLAGCRCVE LDCWDGKGED QEPIITHGKA MCTDILFKDV 130 140 150 160 170 180 IQAIKETAFV TSEYPVILSF ENHCSKYQQY KMSKYCEDLF GDLLLKQALE SHPLEPGRPL 190 200 210 220 230 240 PSPNDLKRKI LIKKQTTETE VEKKQLEALK SMMEAGESAA PVNMLEDDNE EEIESAEQEE 250 260 270 280 290 300 EAHPEYKYGN ELSADDLGHK EAIANSVKKG LVTVEDEQAW MASYKYVGAT TNIHPYLSTM 310 320 330 340 350 360 INYAQPVKFQ GFHVAEERNI HYNMSSFNES VGLGYLKTHA IEFVNYNKRQ MSRIYPKGGR 370 380 390 400 410 420 VDSSNYMPQI FWNSGCQMVS LNYQTPDLAM QLNQGKFEYN GSCGYLLKPD FMRRPDRTFD 430 440 450 460 470 480 PFSETPVDGV IAATCSVQVI SGQFLSDKKI GTYVEVDMYG LPTDTIRKEF RTRMVMNNGL 490 500 510 520 530 540 NPVYNEESFV FRKVILPDLA VLRIAVYDDN NKLIGQRILP LDGLQAGYRH ISLRNEGNKP 550 560 570 580 590 600 LSLPTIFCNI VLKTYVPDGF GDIVDALSDP KKFLSITEKR ADQMRAMGIE TSDIADVPSD 610 620 630 640 650 660 TSKNDKKGKA NTAKANVTPQ SSSELRPTTT AALGAGLEAK KGIELIPQVR IEDLKQMKAY 670 680 690 700 710 720 LKHLKKQQKE LSSLKKKHAK EHSTMQKLHC TQVDKIVAQY DKEKLTHEKI LEKAMKKKGG 730 740 750 760 770 780 SNCLEMKKET EIKIQTLTSD HKSKVKEIVA QHTKEWSDMI NTHSAEEQEI RDLLLSQQCE 790 800 810 820 830 840 LLRKLLISAH EQQTQQLKLS HDRESKEMRA HQAKISMENS KAISQDKSIK NKAERERRVR 850 860 870 880 890 900 ELNSSNTKKF LEERKRLAMK QSKEMDQLKK VQLEHLEFVE KQNEQAKEMQ QMVKLEAEMD RRPATVV « Hide
Isoform 2B [UniParc]. Checksum: C325945560F64E65 Show »	FASTA	919	105,054
10 20 30 40 50 60 MQIIEMYEPD EDLKKQGLIS SDGFCRYLMS DENAPVFLDR LELYQEMDHP LAHYFISSSH 70 80 90 100 110 120 NTYLTGRQFG GKSSVEMYRQ VLLAGCRCVE LDCWDGKGED QEPIITHGKA MCTDILFKDV 130 140 150 160 170 180 IQAIKETAFV TSEYPVILSF ENHCSKYQQY KMSKYCEDLF GDLLLKQALE SHPLEPGRPL 190 200 210 220 230 240 PSPNDLKRKI LIKKQTTETE VEKKQLEALK SMMEAGESAA PVNMLEDDNE EEIESAEQEE 250 260 270 280 290 300 EAHPEYKYGN ELSADDLGHK EAIANSVKKA SDDLEHENSK KGLVTVEDEQ AWMASYKYVG 310 320 330 340 350 360 ATTNIHPYLS TMINYAQPVK FQGFHVAEER NIHYNMSSFN ESVGLGYLKT HAIEFVNYNK 370 380 390 400 410 420 RQMSRIYPKG GRVDSSNYMP QIFWNSGCQM VSLNYQTPDL AMQLNQGKFE YNGSCGYLLK 430 440 450 460 470 480 PDFMRRPDRT FDPFSETPVD GVIAATCSVQ VISGQFLSDK KIGTYVEVDM YGLPTDTIRK 490 500 510 520 530 540 EFRTRMVMNN GLNPVYNEES FVFRKVILPD LAVLRIAVYD DNNKLIGQRI LPLDGLQAGY 550 560 570 580 590 600 RHISLRNEGN KPLSLPTIFC NIVLKTYVPD GFGDIVDALS DPKKFLSITE KRADQMRAMG 610 620 630 640 650 660 IETSDIADVP SDTSKNDKKG KANTAKANVT PQSSSELRPT TTAALGAGLE AKKGIELIPQ 670 680 690 700 710 720 VRIEDLKQMK AYLKHLKKQQ KELSSLKKKH AKEHSTMQKL HCTQVDKIVA QYDKEKLTHE 730 740 750 760 770 780 KILEKAMKKK GGSNCLEMKK ETEIKIQTLT SDHKSKVKEI VAQHTKEWSD MINTHSAEEQ 790 800 810 820 830 840 EIRDLLLSQQ CELLRKLLIS AHEQQTQQLK LSHDRESKEM RAHQAKISME NSKAISQDKS 850 860 870 880 890 900 IKNKAERERR VRELNSSNTK KFLEERKRLA MKQSKEMDQL KKVQLEHLEF VEKQNEQAKE 910 MQQMVKLEAE MDRRPATVV « Hide

References

[1]	"Distinctive subtypes of bovine phospholipase C that have preferential expression in the retina and high homology to the norpA gene product of Drosophila." Ferreira P.A., Shortridge R.D., Pak W.L. Proc. Natl. Acad. Sci. U.S.A. 90:6042-6046(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A; 1B; 2A AND 2B). Tissue: Retina.
[2]	"Purification of a novel phospholipase C isozyme from bovine cerebellum." Min D.S., Kim D.M., Lee Y.H., Seo J., Suh P.-G., Ryu S.H. J. Biol. Chem. 268:12207-12212(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-37; 259-269; 315-337; 386-391; 604-623 AND 634-639. Tissue: Cerebellum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L13936 mRNA. Translation: AAA30699.1. L13935 mRNA. Translation: AAC37304.1. L13937 mRNA. Translation: AAA30700.1. L13938 mRNA. Translation: AAA30701.1.
IPI	IPI00696189. IPI00708128. IPI00708692. IPI00911649.
PIR	B38932.
UniGene	Bt.4552.
3D structure databases
ProteinModelPortal	Q07722.
ModBase	Search...
Proteomic databases
PRIDE	Q07722.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG149692.
HOGENOM	HOG000232046.
HOVERGEN	HBG053609.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 3.20.20.190. 2 hits.
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR001192. Pinositol_PLipase_C. IPR016280. PLC-beta. IPR009535. PLC-beta_CS. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR015359. PLipase_C_EF-hand-like. IPR000909. PLipase_C_PInositol-sp_X_dom. IPR001711. PLipase_C_Pinositol-sp_Y. [Graphical view]
PANTHER	PTHR10336. PTHR10336. 1 hit.
Pfam	PF00168. C2. 1 hit. PF06631. DUF1154. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PIRSF	PIRSF000956. PLC-beta. 1 hit.
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCB4_BOVIN

Accession

Primary (citable) accession number: Q07722
Secondary accession number(s): Q07721, Q07723, Q07724

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents