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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4

Gene

PLCB4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This form has a role in retina signal transduction.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641PROSITE-ProRule annotation
Active sitei211 – 2111PROSITE-ProRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 (EC:3.1.4.11)
Alternative name(s):
PCL-C1
Phosphoinositide phospholipase C-beta-4
Phospholipase C-beta-4
Short name:
PLC-beta-4
Gene namesi
Name:PLCB4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 1023›10231-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4PRO_0000088494Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei734 – 7341PhosphothreonineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ07722.

Expressioni

Tissue specificityi

Preferentially expressed in the retina.

Structurei

3D structure databases

ProteinModelPortaliQ07722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini149 – 299151PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini413 – 529117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini536 – 63499C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiQ07722.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Fragment.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1B (identifier: Q07722-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
PVRSITRTFA SGKTEKVIFQ ALKELGLPSG KNDEIEPAAF TYEKFYELTQ
60 70 80 90 100
KICPRTDIED LFKKINGDKT DYLTVDQLVS FLNEHQRDPR LNEILFPFYD
110 120 130 140 150
AKRAMQIIEM YEPDEDLKKQ GLISSDGFCR YLMSDENAPV FLDRLELYQE
160 170 180 190 200
MDHPLAHYFI SSSHNTYLTG RQFGGKSSVE MYRQVLLAGC RCVELDCWDG
210 220 230 240 250
KGEDQEPIIT HGKAMCTDIL FKDVIQAIKE TAFVTSEYPV ILSFENHCSK
260 270 280 290 300
YQQYKMSKYC EDLFGDLLLK QALESHPLEP GRPLPSPNDL KRKILIKKQT
310 320 330 340 350
TETEVEKKQL EALKSMMEAG ESAAPVNMLE DDNEEEIESA EQEEEAHPEY
360 370 380 390 400
KYGNELSADD LGHKEAIANS VKKASDDLEH ENSKKGLVTV EDEQAWMASY
410 420 430 440 450
KYVGATTNIH PYLSTMINYA QPVKFQGFHV AEERNIHYNM SSFNESVGLG
460 470 480 490 500
YLKTHAIEFV NYNKRQMSRI YPKGGRVDSS NYMPQIFWNS GCQMVSLNYQ
510 520 530 540 550
TPDLAMQLNQ GKFEYNGSCG YLLKPDFMRR PDRTFDPFSE TPVDGVIAAT
560 570 580 590 600
CSVQVISGQF LSDKKIGTYV EVDMYGLPTD TIRKEFRTRM VMNNGLNPVY
610 620 630 640 650
NEESFVFRKV ILPDLAVLRI AVYDDNNKLI GQRILPLDGL QAGYRHISLR
660 670 680 690 700
NEGNKPLSLP TIFCNIVLKT YVPDGFGDIV DALSDPKKFL SITEKRADQM
710 720 730 740 750
RAMGIETSDI ADVPSDTSKN DKKGKANTAK ANVTPQSSSE LRPTTTAALG
760 770 780 790 800
AGLEAKKGIE LIPQVRIEDL KQMKAYLKHL KKQQKELSSL KKKHAKEHST
810 820 830 840 850
MQKLHCTQVD KIVAQYDKEK LTHEKILEKA MKKKGGSNCL EMKKETEIKI
860 870 880 890 900
QTLTSDHKSK VKEIVAQHTK EWSDMINTHS AEEQEIRDLL LSQQCELLRK
910 920 930 940 950
LLISAHEQQT QQLKLSHDRE SKEMRAHQAK ISMENSKAIS QDKSIKNKAE
960 970 980 990 1000
RERRVRELNS SNTKKFLEER KRLAMKQSKE MDQLKKVQLE HLEFVEKQNE
1010 1020
QAKEMQQMVK LEAEMDRRPA TVV
Length:1,023
Mass (Da):117,110
Last modified:November 1, 1996 - v1
Checksum:i416D700C2095748C
GO
Isoform 1A (identifier: Q07722-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     372-383: Missing.

Show »
Length:1,011
Mass (Da):115,755
Checksum:iFCC5C77F5A16FC06
GO
Isoform 2A (identifier: Q07722-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.
     372-383: Missing.

Show »
Length:907
Mass (Da):103,699
Checksum:i60FF44549D54DB2E
GO
Isoform 2B (identifier: Q07722-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.

Show »
Length:919
Mass (Da):105,054
Checksum:iC325945560F64E65
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti24 – 241E → D AA sequence (PubMed:8389372).Curated
Sequence conflicti32 – 321N → D AA sequence (PubMed:8389372).Curated
Sequence conflicti36 – 361E → L AA sequence (PubMed:8389372).Curated
Sequence conflicti328 – 3281M → I AA sequence (PubMed:8389372).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei‹1 – 104›104Missing in isoform 2A and isoform 2B. 1 PublicationVSP_004719Add
BLAST
Alternative sequencei372 – 38312Missing in isoform 1A and isoform 2A. 1 PublicationVSP_004720Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13936 mRNA. Translation: AAA30699.1.
L13935 mRNA. Translation: AAC37304.1.
L13937 mRNA. Translation: AAA30700.1.
L13938 mRNA. Translation: AAA30701.1.
PIRiB38932.
UniGeneiBt.4552.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13936 mRNA. Translation: AAA30699.1.
L13935 mRNA. Translation: AAC37304.1.
L13937 mRNA. Translation: AAA30700.1.
L13938 mRNA. Translation: AAA30701.1.
PIRiB38932.
UniGeneiBt.4552.

3D structure databases

ProteinModelPortaliQ07722.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ07722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiQ07722.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Distinctive subtypes of bovine phospholipase C that have preferential expression in the retina and high homology to the norpA gene product of Drosophila."
    Ferreira P.A., Shortridge R.D., Pak W.L.
    Proc. Natl. Acad. Sci. U.S.A. 90:6042-6046(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A; 1B; 2A AND 2B).
    Tissue: Retina.
  2. "Purification of a novel phospholipase C isozyme from bovine cerebellum."
    Min D.S., Kim D.M., Lee Y.H., Seo J., Suh P.-G., Ryu S.H.
    J. Biol. Chem. 268:12207-12212(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-37; 259-269; 315-337; 386-391; 604-623 AND 634-639.
    Tissue: Cerebellum.

Entry informationi

Entry nameiPLCB4_BOVIN
AccessioniPrimary (citable) accession number: Q07722
Secondary accession number(s): Q07721, Q07723, Q07724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.