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Q07722 (PLCB4_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4

EC=3.1.4.11
Alternative name(s):
PCL-C1
Phosphoinositide phospholipase C-beta-4
Phospholipase C-beta-4
Short name=PLC-beta-4
Gene names
Name:PLCB4
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length1023 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This form has a role in retina signal transduction.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Calcium.

Tissue specificity

Preferentially expressed in the retina.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Contains 1 C2 domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1B (identifier: Q07722-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1A (identifier: Q07722-2)

The sequence of this isoform differs from the canonical sequence as follows:
     372-383: Missing.
Isoform 2A (identifier: Q07722-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.
     372-383: Missing.
Isoform 2B (identifier: Q07722-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 1023›10231-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
PRO_0000088494

Regions

Domain149 – 299151PI-PLC X-box
Domain413 – 529117PI-PLC Y-box
Domain536 – 63499C2

Sites

Active site1641 By similarity
Active site2111 By similarity

Amino acid modifications

Modified residue7341Phosphothreonine By similarity

Natural variations

Alternative sequence‹1 – 104›104Missing in isoform 2A and isoform 2B.
VSP_004719
Alternative sequence372 – 38312Missing in isoform 1A and isoform 2A.
VSP_004720

Experimental info

Sequence conflict241E → D AA sequence Ref.2
Sequence conflict321N → D AA sequence Ref.2
Sequence conflict361E → L AA sequence Ref.2
Sequence conflict3281M → I AA sequence Ref.2
Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Isoform 1B [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 416D700C2095748C

FASTA1,023117,110
        10         20         30         40         50         60 
PVRSITRTFA SGKTEKVIFQ ALKELGLPSG KNDEIEPAAF TYEKFYELTQ KICPRTDIED 

        70         80         90        100        110        120 
LFKKINGDKT DYLTVDQLVS FLNEHQRDPR LNEILFPFYD AKRAMQIIEM YEPDEDLKKQ 

       130        140        150        160        170        180 
GLISSDGFCR YLMSDENAPV FLDRLELYQE MDHPLAHYFI SSSHNTYLTG RQFGGKSSVE 

       190        200        210        220        230        240 
MYRQVLLAGC RCVELDCWDG KGEDQEPIIT HGKAMCTDIL FKDVIQAIKE TAFVTSEYPV 

       250        260        270        280        290        300 
ILSFENHCSK YQQYKMSKYC EDLFGDLLLK QALESHPLEP GRPLPSPNDL KRKILIKKQT 

       310        320        330        340        350        360 
TETEVEKKQL EALKSMMEAG ESAAPVNMLE DDNEEEIESA EQEEEAHPEY KYGNELSADD 

       370        380        390        400        410        420 
LGHKEAIANS VKKASDDLEH ENSKKGLVTV EDEQAWMASY KYVGATTNIH PYLSTMINYA 

       430        440        450        460        470        480 
QPVKFQGFHV AEERNIHYNM SSFNESVGLG YLKTHAIEFV NYNKRQMSRI YPKGGRVDSS 

       490        500        510        520        530        540 
NYMPQIFWNS GCQMVSLNYQ TPDLAMQLNQ GKFEYNGSCG YLLKPDFMRR PDRTFDPFSE 

       550        560        570        580        590        600 
TPVDGVIAAT CSVQVISGQF LSDKKIGTYV EVDMYGLPTD TIRKEFRTRM VMNNGLNPVY 

       610        620        630        640        650        660 
NEESFVFRKV ILPDLAVLRI AVYDDNNKLI GQRILPLDGL QAGYRHISLR NEGNKPLSLP 

       670        680        690        700        710        720 
TIFCNIVLKT YVPDGFGDIV DALSDPKKFL SITEKRADQM RAMGIETSDI ADVPSDTSKN 

       730        740        750        760        770        780 
DKKGKANTAK ANVTPQSSSE LRPTTTAALG AGLEAKKGIE LIPQVRIEDL KQMKAYLKHL 

       790        800        810        820        830        840 
KKQQKELSSL KKKHAKEHST MQKLHCTQVD KIVAQYDKEK LTHEKILEKA MKKKGGSNCL 

       850        860        870        880        890        900 
EMKKETEIKI QTLTSDHKSK VKEIVAQHTK EWSDMINTHS AEEQEIRDLL LSQQCELLRK 

       910        920        930        940        950        960 
LLISAHEQQT QQLKLSHDRE SKEMRAHQAK ISMENSKAIS QDKSIKNKAE RERRVRELNS 

       970        980        990       1000       1010       1020 
SNTKKFLEER KRLAMKQSKE MDQLKKVQLE HLEFVEKQNE QAKEMQQMVK LEAEMDRRPA 


TVV 

« Hide

Isoform 1A [UniParc].

Checksum: FCC5C77F5A16FC06
Show »

FASTA1,011115,755
Isoform 2A [UniParc].

Checksum: 60FF44549D54DB2E
Show »

FASTA907103,699
Isoform 2B [UniParc].

Checksum: C325945560F64E65
Show »

FASTA919105,054

References

[1]"Distinctive subtypes of bovine phospholipase C that have preferential expression in the retina and high homology to the norpA gene product of Drosophila."
Ferreira P.A., Shortridge R.D., Pak W.L.
Proc. Natl. Acad. Sci. U.S.A. 90:6042-6046(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A; 1B; 2A AND 2B).
Tissue: Retina.
[2]"Purification of a novel phospholipase C isozyme from bovine cerebellum."
Min D.S., Kim D.M., Lee Y.H., Seo J., Suh P.-G., Ryu S.H.
J. Biol. Chem. 268:12207-12212(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-37; 259-269; 315-337; 386-391; 604-623 AND 634-639.
Tissue: Cerebellum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L13936 mRNA. Translation: AAA30699.1.
L13935 mRNA. Translation: AAC37304.1.
L13937 mRNA. Translation: AAA30700.1.
L13938 mRNA. Translation: AAA30701.1.
PIRB38932.
UniGeneBt.4552.

3D structure databases

ProteinModelPortalQ07722.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ07722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG149692.
HOGENOMHOG000232046.
HOVERGENHBG053609.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028404. PLC_beta4/NorpA.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF8. PTHR10336:SF8. 1 hit.
PfamPF00168. C2. 1 hit.
PF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PIRSFPIRSF000956. PLC-beta. 1 hit.
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLCB4_BOVIN
AccessionPrimary (citable) accession number: Q07722
Secondary accession number(s): Q07721, Q07723, Q07724
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families