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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4

Gene

PLCB4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This form has a role in retina signal transduction.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei164PROSITE-ProRule annotation1
Active sitei211PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium

Chemistry databases

SwissLipidsiSLP:000000950

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 (EC:3.1.4.11)
Alternative name(s):
PCL-C1
Phosphoinositide phospholipase C-beta-4
Phospholipase C-beta-4
Short name:
PLC-beta-4
Gene namesi
Name:PLCB4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000088494‹1 – 10231-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4Add BLAST›1023

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei734PhosphothreonineBy similarity1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ07722
PRIDEiQ07722

Expressioni

Tissue specificityi

Preferentially expressed in the retina.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017447

Structurei

3D structure databases

ProteinModelPortaliQ07722
SMRiQ07722
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 299PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST151
Domaini413 – 529PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini536 – 634C2PROSITE-ProRule annotationAdd BLAST99

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
HOGENOMiHOG000232046
HOVERGENiHBG053609
InParanoidiQ07722

Family and domain databases

Gene3Di2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR016280 PLC-beta
IPR009535 PLC-beta_CS
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF06631 DUF1154, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PIRSFiPIRSF000956 PLC-beta, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequences (4)i

Sequence statusi: Fragment.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1B (identifier: Q07722-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
PVRSITRTFA SGKTEKVIFQ ALKELGLPSG KNDEIEPAAF TYEKFYELTQ
60 70 80 90 100
KICPRTDIED LFKKINGDKT DYLTVDQLVS FLNEHQRDPR LNEILFPFYD
110 120 130 140 150
AKRAMQIIEM YEPDEDLKKQ GLISSDGFCR YLMSDENAPV FLDRLELYQE
160 170 180 190 200
MDHPLAHYFI SSSHNTYLTG RQFGGKSSVE MYRQVLLAGC RCVELDCWDG
210 220 230 240 250
KGEDQEPIIT HGKAMCTDIL FKDVIQAIKE TAFVTSEYPV ILSFENHCSK
260 270 280 290 300
YQQYKMSKYC EDLFGDLLLK QALESHPLEP GRPLPSPNDL KRKILIKKQT
310 320 330 340 350
TETEVEKKQL EALKSMMEAG ESAAPVNMLE DDNEEEIESA EQEEEAHPEY
360 370 380 390 400
KYGNELSADD LGHKEAIANS VKKASDDLEH ENSKKGLVTV EDEQAWMASY
410 420 430 440 450
KYVGATTNIH PYLSTMINYA QPVKFQGFHV AEERNIHYNM SSFNESVGLG
460 470 480 490 500
YLKTHAIEFV NYNKRQMSRI YPKGGRVDSS NYMPQIFWNS GCQMVSLNYQ
510 520 530 540 550
TPDLAMQLNQ GKFEYNGSCG YLLKPDFMRR PDRTFDPFSE TPVDGVIAAT
560 570 580 590 600
CSVQVISGQF LSDKKIGTYV EVDMYGLPTD TIRKEFRTRM VMNNGLNPVY
610 620 630 640 650
NEESFVFRKV ILPDLAVLRI AVYDDNNKLI GQRILPLDGL QAGYRHISLR
660 670 680 690 700
NEGNKPLSLP TIFCNIVLKT YVPDGFGDIV DALSDPKKFL SITEKRADQM
710 720 730 740 750
RAMGIETSDI ADVPSDTSKN DKKGKANTAK ANVTPQSSSE LRPTTTAALG
760 770 780 790 800
AGLEAKKGIE LIPQVRIEDL KQMKAYLKHL KKQQKELSSL KKKHAKEHST
810 820 830 840 850
MQKLHCTQVD KIVAQYDKEK LTHEKILEKA MKKKGGSNCL EMKKETEIKI
860 870 880 890 900
QTLTSDHKSK VKEIVAQHTK EWSDMINTHS AEEQEIRDLL LSQQCELLRK
910 920 930 940 950
LLISAHEQQT QQLKLSHDRE SKEMRAHQAK ISMENSKAIS QDKSIKNKAE
960 970 980 990 1000
RERRVRELNS SNTKKFLEER KRLAMKQSKE MDQLKKVQLE HLEFVEKQNE
1010 1020
QAKEMQQMVK LEAEMDRRPA TVV
Length:1,023
Mass (Da):117,110
Last modified:November 1, 1996 - v1
Checksum:i416D700C2095748C
GO
Isoform 1A (identifier: Q07722-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     372-383: Missing.

Show »
Length:1,011
Mass (Da):115,755
Checksum:iFCC5C77F5A16FC06
GO
Isoform 2A (identifier: Q07722-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.
     372-383: Missing.

Show »
Length:907
Mass (Da):103,699
Checksum:i60FF44549D54DB2E
GO
Isoform 2B (identifier: Q07722-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.

Show »
Length:919
Mass (Da):105,054
Checksum:iC325945560F64E65
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence conflicti24E → D AA sequence (PubMed:8389372).Curated1
Sequence conflicti32N → D AA sequence (PubMed:8389372).Curated1
Sequence conflicti36E → L AA sequence (PubMed:8389372).Curated1
Sequence conflicti328M → I AA sequence (PubMed:8389372).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004719‹1 – 104Missing in isoform 2A and isoform 2B. 1 PublicationAdd BLAST›104
Alternative sequenceiVSP_004720372 – 383Missing in isoform 1A and isoform 2A. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13936 mRNA Translation: AAA30699.1
L13935 mRNA Translation: AAC37304.1
L13937 mRNA Translation: AAA30700.1
L13938 mRNA Translation: AAA30701.1
PIRiB38932
UniGeneiBt.4552

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiPLCB4_BOVIN
AccessioniPrimary (citable) accession number: Q07722
Secondary accession number(s): Q07721, Q07723, Q07724
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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