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Q07722

- PLCB4_BOVIN

UniProt

Q07722 - PLCB4_BOVIN

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4

Gene

PLCB4

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This form has a role in retina signal transduction.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641PROSITE-ProRule annotation
    Active sitei211 – 2111PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 (EC:3.1.4.11)
    Alternative name(s):
    PCL-C1
    Phosphoinositide phospholipase C-beta-4
    Phospholipase C-beta-4
    Short name:
    PLC-beta-4
    Gene namesi
    Name:PLCB4
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 1023›10231-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4PRO_0000088494Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei734 – 7341PhosphothreonineBy similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ07722.

    Expressioni

    Tissue specificityi

    Preferentially expressed in the retina.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07722.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini149 – 299151PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini413 – 529117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini536 – 63499C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000232046.
    HOVERGENiHBG053609.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR009535. PLC-beta_CS.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF06631. DUF1154. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000956. PLC-beta. 1 hit.
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Fragment.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1B (identifier: Q07722-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    PVRSITRTFA SGKTEKVIFQ ALKELGLPSG KNDEIEPAAF TYEKFYELTQ     50
    KICPRTDIED LFKKINGDKT DYLTVDQLVS FLNEHQRDPR LNEILFPFYD 100
    AKRAMQIIEM YEPDEDLKKQ GLISSDGFCR YLMSDENAPV FLDRLELYQE 150
    MDHPLAHYFI SSSHNTYLTG RQFGGKSSVE MYRQVLLAGC RCVELDCWDG 200
    KGEDQEPIIT HGKAMCTDIL FKDVIQAIKE TAFVTSEYPV ILSFENHCSK 250
    YQQYKMSKYC EDLFGDLLLK QALESHPLEP GRPLPSPNDL KRKILIKKQT 300
    TETEVEKKQL EALKSMMEAG ESAAPVNMLE DDNEEEIESA EQEEEAHPEY 350
    KYGNELSADD LGHKEAIANS VKKASDDLEH ENSKKGLVTV EDEQAWMASY 400
    KYVGATTNIH PYLSTMINYA QPVKFQGFHV AEERNIHYNM SSFNESVGLG 450
    YLKTHAIEFV NYNKRQMSRI YPKGGRVDSS NYMPQIFWNS GCQMVSLNYQ 500
    TPDLAMQLNQ GKFEYNGSCG YLLKPDFMRR PDRTFDPFSE TPVDGVIAAT 550
    CSVQVISGQF LSDKKIGTYV EVDMYGLPTD TIRKEFRTRM VMNNGLNPVY 600
    NEESFVFRKV ILPDLAVLRI AVYDDNNKLI GQRILPLDGL QAGYRHISLR 650
    NEGNKPLSLP TIFCNIVLKT YVPDGFGDIV DALSDPKKFL SITEKRADQM 700
    RAMGIETSDI ADVPSDTSKN DKKGKANTAK ANVTPQSSSE LRPTTTAALG 750
    AGLEAKKGIE LIPQVRIEDL KQMKAYLKHL KKQQKELSSL KKKHAKEHST 800
    MQKLHCTQVD KIVAQYDKEK LTHEKILEKA MKKKGGSNCL EMKKETEIKI 850
    QTLTSDHKSK VKEIVAQHTK EWSDMINTHS AEEQEIRDLL LSQQCELLRK 900
    LLISAHEQQT QQLKLSHDRE SKEMRAHQAK ISMENSKAIS QDKSIKNKAE 950
    RERRVRELNS SNTKKFLEER KRLAMKQSKE MDQLKKVQLE HLEFVEKQNE 1000
    QAKEMQQMVK LEAEMDRRPA TVV 1023
    Length:1,023
    Mass (Da):117,110
    Last modified:November 1, 1996 - v1
    Checksum:i416D700C2095748C
    GO
    Isoform 1A (identifier: Q07722-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         372-383: Missing.

    Show »
    Length:1,011
    Mass (Da):115,755
    Checksum:iFCC5C77F5A16FC06
    GO
    Isoform 2A (identifier: Q07722-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-104: Missing.
         372-383: Missing.

    Show »
    Length:907
    Mass (Da):103,699
    Checksum:i60FF44549D54DB2E
    GO
    Isoform 2B (identifier: Q07722-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-104: Missing.

    Show »
    Length:919
    Mass (Da):105,054
    Checksum:iC325945560F64E65
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Sequence conflicti24 – 241E → D AA sequence (PubMed:8389372)Curated
    Sequence conflicti32 – 321N → D AA sequence (PubMed:8389372)Curated
    Sequence conflicti36 – 361E → L AA sequence (PubMed:8389372)Curated
    Sequence conflicti328 – 3281M → I AA sequence (PubMed:8389372)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei‹1 – 104›104Missing in isoform 2A and isoform 2B. 1 PublicationVSP_004719Add
    BLAST
    Alternative sequencei372 – 38312Missing in isoform 1A and isoform 2A. 1 PublicationVSP_004720Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13936 mRNA. Translation: AAA30699.1.
    L13935 mRNA. Translation: AAC37304.1.
    L13937 mRNA. Translation: AAA30700.1.
    L13938 mRNA. Translation: AAA30701.1.
    PIRiB38932.
    UniGeneiBt.4552.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13936 mRNA. Translation: AAA30699.1 .
    L13935 mRNA. Translation: AAC37304.1 .
    L13937 mRNA. Translation: AAA30700.1 .
    L13938 mRNA. Translation: AAA30701.1 .
    PIRi B38932.
    UniGenei Bt.4552.

    3D structure databases

    ProteinModelPortali Q07722.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q07722.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000232046.
    HOVERGENi HBG053609.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR009535. PLC-beta_CS.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF06631. DUF1154. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000956. PLC-beta. 1 hit.
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Distinctive subtypes of bovine phospholipase C that have preferential expression in the retina and high homology to the norpA gene product of Drosophila."
      Ferreira P.A., Shortridge R.D., Pak W.L.
      Proc. Natl. Acad. Sci. U.S.A. 90:6042-6046(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A; 1B; 2A AND 2B).
      Tissue: Retina.
    2. "Purification of a novel phospholipase C isozyme from bovine cerebellum."
      Min D.S., Kim D.M., Lee Y.H., Seo J., Suh P.-G., Ryu S.H.
      J. Biol. Chem. 268:12207-12212(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-37; 259-269; 315-337; 386-391; 604-623 AND 634-639.
      Tissue: Cerebellum.

    Entry informationi

    Entry nameiPLCB4_BOVIN
    AccessioniPrimary (citable) accession number: Q07722
    Secondary accession number(s): Q07721, Q07723, Q07724
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3