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Q07722

- PLCB4_BOVIN

UniProt

Q07722 - PLCB4_BOVIN

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4

Gene
PLCB4
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This form has a role in retina signal transduction.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Calcium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641 By similarity
Active sitei211 – 2111 By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 (EC:3.1.4.11)
Alternative name(s):
PCL-C1
Phosphoinositide phospholipase C-beta-4
Phospholipase C-beta-4
Short name:
PLC-beta-4
Gene namesi
Name:PLCB4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 1023›10231-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4PRO_0000088494Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei734 – 7341Phosphothreonine By similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ07722.

Expressioni

Tissue specificityi

Preferentially expressed in the retina.

Structurei

3D structure databases

ProteinModelPortaliQ07722.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini149 – 299151PI-PLC X-boxAdd
BLAST
Domaini413 – 529117PI-PLC Y-boxAdd
BLAST
Domaini536 – 63499C2Add
BLAST

Sequence similaritiesi

Contains 1 C2 domain.

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Fragment.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1B (identifier: Q07722-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

PVRSITRTFA SGKTEKVIFQ ALKELGLPSG KNDEIEPAAF TYEKFYELTQ     50
KICPRTDIED LFKKINGDKT DYLTVDQLVS FLNEHQRDPR LNEILFPFYD 100
AKRAMQIIEM YEPDEDLKKQ GLISSDGFCR YLMSDENAPV FLDRLELYQE 150
MDHPLAHYFI SSSHNTYLTG RQFGGKSSVE MYRQVLLAGC RCVELDCWDG 200
KGEDQEPIIT HGKAMCTDIL FKDVIQAIKE TAFVTSEYPV ILSFENHCSK 250
YQQYKMSKYC EDLFGDLLLK QALESHPLEP GRPLPSPNDL KRKILIKKQT 300
TETEVEKKQL EALKSMMEAG ESAAPVNMLE DDNEEEIESA EQEEEAHPEY 350
KYGNELSADD LGHKEAIANS VKKASDDLEH ENSKKGLVTV EDEQAWMASY 400
KYVGATTNIH PYLSTMINYA QPVKFQGFHV AEERNIHYNM SSFNESVGLG 450
YLKTHAIEFV NYNKRQMSRI YPKGGRVDSS NYMPQIFWNS GCQMVSLNYQ 500
TPDLAMQLNQ GKFEYNGSCG YLLKPDFMRR PDRTFDPFSE TPVDGVIAAT 550
CSVQVISGQF LSDKKIGTYV EVDMYGLPTD TIRKEFRTRM VMNNGLNPVY 600
NEESFVFRKV ILPDLAVLRI AVYDDNNKLI GQRILPLDGL QAGYRHISLR 650
NEGNKPLSLP TIFCNIVLKT YVPDGFGDIV DALSDPKKFL SITEKRADQM 700
RAMGIETSDI ADVPSDTSKN DKKGKANTAK ANVTPQSSSE LRPTTTAALG 750
AGLEAKKGIE LIPQVRIEDL KQMKAYLKHL KKQQKELSSL KKKHAKEHST 800
MQKLHCTQVD KIVAQYDKEK LTHEKILEKA MKKKGGSNCL EMKKETEIKI 850
QTLTSDHKSK VKEIVAQHTK EWSDMINTHS AEEQEIRDLL LSQQCELLRK 900
LLISAHEQQT QQLKLSHDRE SKEMRAHQAK ISMENSKAIS QDKSIKNKAE 950
RERRVRELNS SNTKKFLEER KRLAMKQSKE MDQLKKVQLE HLEFVEKQNE 1000
QAKEMQQMVK LEAEMDRRPA TVV 1023
Length:1,023
Mass (Da):117,110
Last modified:November 1, 1996 - v1
Checksum:i416D700C2095748C
GO
Isoform 1A (identifier: Q07722-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     372-383: Missing.

Show »
Length:1,011
Mass (Da):115,755
Checksum:iFCC5C77F5A16FC06
GO
Isoform 2A (identifier: Q07722-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.
     372-383: Missing.

Show »
Length:907
Mass (Da):103,699
Checksum:i60FF44549D54DB2E
GO
Isoform 2B (identifier: Q07722-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.

Show »
Length:919
Mass (Da):105,054
Checksum:iC325945560F64E65
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei‹1 – 104›104Missing in isoform 2A and isoform 2B. VSP_004719Add
BLAST
Alternative sequencei372 – 38312Missing in isoform 1A and isoform 2A. VSP_004720Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241E → D AA sequence 1 Publication
Sequence conflicti32 – 321N → D AA sequence 1 Publication
Sequence conflicti36 – 361E → L AA sequence 1 Publication
Sequence conflicti328 – 3281M → I AA sequence 1 Publication

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L13936 mRNA. Translation: AAA30699.1.
L13935 mRNA. Translation: AAC37304.1.
L13937 mRNA. Translation: AAA30700.1.
L13938 mRNA. Translation: AAA30701.1.
PIRiB38932.
UniGeneiBt.4552.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L13936 mRNA. Translation: AAA30699.1 .
L13935 mRNA. Translation: AAC37304.1 .
L13937 mRNA. Translation: AAA30700.1 .
L13938 mRNA. Translation: AAA30701.1 .
PIRi B38932.
UniGenei Bt.4552.

3D structure databases

ProteinModelPortali Q07722.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q07722.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi NOG149692.
HOGENOMi HOG000232046.
HOVERGENi HBG053609.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
Pfami PF00168. C2. 1 hit.
PF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PIRSFi PIRSF000956. PLC-beta. 1 hit.
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Distinctive subtypes of bovine phospholipase C that have preferential expression in the retina and high homology to the norpA gene product of Drosophila."
    Ferreira P.A., Shortridge R.D., Pak W.L.
    Proc. Natl. Acad. Sci. U.S.A. 90:6042-6046(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A; 1B; 2A AND 2B).
    Tissue: Retina.
  2. "Purification of a novel phospholipase C isozyme from bovine cerebellum."
    Min D.S., Kim D.M., Lee Y.H., Seo J., Suh P.-G., Ryu S.H.
    J. Biol. Chem. 268:12207-12212(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-37; 259-269; 315-337; 386-391; 604-623 AND 634-639.
    Tissue: Cerebellum.

Entry informationi

Entry nameiPLCB4_BOVIN
AccessioniPrimary (citable) accession number: Q07722
Secondary accession number(s): Q07721, Q07723, Q07724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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