Reviewed, UniProtKB/Swiss-Prot Q07703 (METC_BORAV)

Names and origin

Protein names

Recommended name:
    Cystathionine beta-lyase
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase
    Osteotoxin

Gene names

Name:

metC

Organism

Bordetella avium

Taxonomic identifier

521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Also has cytotoxic activity toward osteogenic, osteosarcoma and tracheal cells, in vitro. The chemical basis for cell toxicity might be the formation and subsequent transfer of sulfane-sulfur to proteins, derived via beta-cystathionase cleavage of L-cystine.
Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 396	395	Cystathionine beta-lyase		PRO_0000114767
Amino acid modifications
	Modified residue	214	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07703-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F0D11DA6E108F064	FASTA	396	42,610
	10 20 30 40 50 60 MSDTSAKHID TLLQHLGSAP FNPDTGAAPV NLPSVRASTV RFQSLAKLED AQRRKAAGER 70 80 90 100 110 120 ASTYGRMGMD THAALEQVFA ELEGGTHCYL ASSGLAGISM VFLSLLSAGE HALVADCAYG 130 140 150 160 170 180 PVHELHEAVL SRLGIDVTFF DAKADLASLV RPTTRLIFAE APGSLLFEML DMPALARFAK 190 200 210 220 230 240 QHDLILATDN TWGSGYIYRP LTLGAQVSVI AGTKYVGGHS DLMLGAVVTN DEAIAKRLNR 250 260 270 280 290 300 TQYALGYSVS ADDAWLALRG VRTMPVRMAQ HARHALEVCE FLQNRPEVVR LYHPAWPADP 310 320 330 340 350 360 GHALWQRDCS GSNGMLAVQL GLSPQAARDF VNALTLFGIG FSWGGFESLV QLVTPGELAR 370 380 390 HQYWQGGSDA LVRLHIGLES PADLIADLAQ ALDRAA

References

[1]	"Toxicity of Bordetella avium beta-cystathionase toward MC3T3-E1 osteogenic cells." Gentry-Weeks C.R., Keith J.M., Thompson J. J. Biol. Chem. 268:7298-7314(1993) [PubMed: 8463265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-33. Strain: 197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10425 Genomic DNA. Translation: AAA22978.1.
PIR	A46084.
3D structure databases
SMR	Q07703. Positions 9-395.
ModBase	Search...
Phylogenomic databases
PhylomeDB	Q07703.
Enzyme and pathway databases
BRENDA	4.4.1.8. 21480.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR006233. Cys_b_lyase_bac. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808:SF8. Cys_b_lyase_bac. 1 hit. PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
TIGRFAMs	TIGR01324. cysta_beta_ly_B. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METC_BORAV

Accession

Primary (citable) accession number: Q07703

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)