Reviewed, UniProtKB/Swiss-Prot Q07702 (GYRA_MYCTU)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	Rv0006, MT0006
ORF Names:	MTCY10H4.04

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Miscellaneous	When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 838	838	DNA gyrase subunit A		PRO_0000145243
Sites
	Active site	129	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Natural variations
	Natural variant	88	1	G → C Confers ciprofloxacin resistance.
	Natural variant	90	1	A → V Confers ciprofloxacin resistance.
	Natural variant	91	1	S → P Confers ciprofloxacin resistance.
	Natural variant	94	1	D → A Confers ciprofloxacin resistance.
	Natural variant	94	1	D → G Confers ciprofloxacin resistance.
	Natural variant	94	1	D → H Confers ciprofloxacin resistance.
	Natural variant	94	1	D → N Confers ciprofloxacin resistance.
	Natural variant	94	1	D → Y Confers ciprofloxacin resistance.
	Natural variant	95	1	S → T
Experimental info
	Sequence conflict	21	1	E → Q in AAK44229. Ref.3
	Sequence conflict	83	1	N → K in AAC36878. Ref.4
	Sequence conflict	668	1	G → D in AAK44229. Ref.3
	Sequence conflict	712	1	V → L Ref.2
	Sequence conflict	712	1	V → L Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q07702-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 913AFE13C2AD7937	FASTA	838	92,260
	10 20 30 40 50 60 MTDTTLPPDD SLDRIEPVDI EQEMQRSYID YAMSVIVGRA LPEVRDGLKP VHRRVLYAMF 70 80 90 100 110 120 DSGFRPDRSH AKSARSVAET MGNYHPHGDA SIYDSLVRMA QPWSLRYPLV DGQGNFGSPG 130 140 150 160 170 180 NDPPAAMRYT EARLTPLAME MLREIDEETV DFIPNYDGRV QEPTVLPSRF PNLLANGSGG 190 200 210 220 230 240 IAVGMATNIP PHNLRELADA VFWALENHDA DEEETLAAVM GRVKGPDFPT AGLIVGSQGT 250 260 270 280 290 300 ADAYKTGRGS IRMRGVVEVE EDSRGRTSLV ITELPYQVNH DNFITSIAEQ VRDGKLAGIS 310 320 330 340 350 360 NIEDQSSDRV GLRIVIEIKR DAVAKVVINN LYKHTQLQTS FGANMLAIVD GVPRTLRLDQ 370 380 390 400 410 420 LIRYYVDHQL DVIVRRTTYR LRKANERAHI LRGLVKALDA LDEVIALIRA SETVDIARAG 430 440 450 460 470 480 LIELLDIDEI QAQAILDMQL RRLAALERQR IIDDLAKIEA EIADLEDILA KPERQRGIVR 490 500 510 520 530 540 DELAEIVDRH GDDRRTRIIA ADGDVSDEDL IAREDVVVTI TETGYAKRTK TDLYRSQKRG 550 560 570 580 590 600 GKGVQGAGLK QDDIVAHFFV CSTHDLILFF TTQGRVYRAK AYDLPEASRT ARGQHVANLL 610 620 630 640 650 660 AFQPEERIAQ VIQIRGYTDA PYLVLATRNG LVKKSKLTDF DSNRSGGIVA VNLRDNDELV 670 680 690 700 710 720 GAVLCSAGDD LLLVSANGQS IRFSATDEAL RPMGRATSGV QGMRFNIDDR LVSLNVVREG 730 740 750 760 770 780 TYLLVATSGG YAKRTAIEEY PVQGRGGKGV LTVMYDRRRG RLVGALIVDD DSELYAVTSG 790 800 810 820 830 GGVIRTAARQ VRKAGRQTKG VRLMNLGEGD TLLAIARNAE ESGDDNAVDA NGADQTGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of Mycobacterium tuberculosis gyrA and gyrB genes and detection of quinolone resistance mutations." Takiff H.E., Salazar L., Guerrero C., Philipp W., Huang W.M., Kreiswirth B., Cole S.T., Jacobs W.R. Jr., Telenti A. Antimicrob. Agents Chemother. 38:773-780(1994) [PubMed: 8031045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"A PCR method for the sequence analysis of the gyrA, polA and rnhA gene segments from mycobacteria." Mizrahi V., Huberts P., Dawes S.S., Dudding L.R. Gene 136:287-290(1993) [PubMed: 8294019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 82-188. Strain: ATCC 25618 / H37Rv.
[5]	"Selection of a gyrA mutant of Mycobacterium tuberculosis resistant to fluoroquinolones during treatment with ofloxacin." Cambau E., Sougakoff W., Besson M., Truffot-Pernot C., Grosset J., Jarlier V. J. Infect. Dis. 170:479-483(1994) [PubMed: 8035042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-124. Strain: ATCC 25618 / H37Rv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27512 Genomic DNA. Translation: AAA83017.1. BX842572 Genomic DNA. Translation: CAB02427.1. AE000516 Genomic DNA. Translation: AAK44229.1. L11919 Unassigned DNA. Translation: AAC36878.1. X72872 Genomic DNA. Translation: CAA51386.1.
PIR	D70698.
RefSeq	NP_214520.1. NP_334415.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Genome annotation databases
GeneID	887105. 922437.
GenomeReviews	Gene locus MT0006 in contig AE000516_GR. Gene locus Rv0006 in contig AL123456_GR.
KEGG	mtc:MT0006. mtu:Rv0006.
TIGR	MT0006.
Organism-specific databases
TubercuList	Rv0006.
Phylogenomic databases
HOGENOM	Q07702.
OMA	SEIQANS.
Enzyme and pathway databases
BRENDA	5.99.1.3. 809.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCTU

Accession

Primary (citable) accession number: Q07702
Secondary accession number(s): P71574, P97136

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)