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Protein

Phosphorelay intermediate protein YPD1

Gene

YPD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorelay intermediate protein that is part of the branched SLN1-YPD1-SKN7/SSK1 two-component regulatory system, which controls activity of the HOG1 pathway and gene expression in response to changes in the osmolarity of the extracellular environment. Catalyzes the phosphoryl group transfer from the membrane-bound osmosensing histidine kinase SLN1 to two distinct response regulator proteins, SSK1 in the cytoplasm, and transcription factor SKN7 in the nucleus.2 Publications

Miscellaneous

Present with 6330 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • histidine phosphotransfer kinase activity Source: GO_Central
  • protein histidine kinase binding Source: SGD
  • transferase activity, transferring phosphorus-containing groups Source: SGD

GO - Biological processi

  • osmosensory signaling via phosphorelay pathway Source: SGD
  • phosphorylation Source: GO_Central

Keywordsi

Biological processTwo-component regulatory system

Enzyme and pathway databases

BioCyciYEAST:G3O-29613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorelay intermediate protein YPD1
Alternative name(s):
Histidine-containing phosphotransfer protein YPD1
Tyrosine phosphatase-dependent protein 1
Gene namesi
Name:YPD1
Ordered Locus Names:YDL235C
ORF Names:D0790
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL235C.
SGDiS000002394. YPD1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64H → Q: Loss of function. 1 Publication1
Mutagenesisi67K → A: Reduces binding of the 4-aspartylphosphate of SLN1. 1 Publication1
Mutagenesisi68G → Q: Reduces phosphoryl transfer rate. 1 Publication1
Mutagenesisi74G → C in NH1; causes resistance to the antifungal antibiotic pradimicin. 1 Publication1
Mutagenesisi86Q → A: Reduces phosphoryl transfer rate. 1 Publication1
Mutagenesisi90R → A: Reduces phosphoryl transfer rate. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002627581 – 167Phosphorelay intermediate protein YPD1Add BLAST167

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64PhosphohistidinePROSITE-ProRule annotation1 Publication1

Post-translational modificationi

The phosphorelay mechanism involves the sequential transfer of a phosphate group from 'His-576' (H1) to 'Asp-1144' (D1) of SLN1, then to His-64 (H2) of YPD1 and finally to 'Asp-554' (D2) of SSK1 or 'Asp-427' (D2) of SKN7.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ07688.
PRIDEiQ07688.
TopDownProteomicsiQ07688.

PTM databases

iPTMnetiQ07688.

Interactioni

Subunit structurei

Interacts with the response regulatory domains of SLN1 and SSK1.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein histidine kinase binding Source: SGD

Protein-protein interaction databases

BioGridi31876. 91 interactors.
DIPiDIP-5899N.
IntActiQ07688. 4 interactors.
MINTiMINT-696217.
STRINGi4932.YDL235C.

Structurei

Secondary structure

1167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 19Combined sources9
Turni20 – 23Combined sources4
Helixi26 – 51Combined sources26
Helixi56 – 72Combined sources17
Helixi76 – 89Combined sources14
Helixi99 – 103Combined sources5
Helixi109 – 112Combined sources4
Beta strandi113 – 115Combined sources3
Turni117 – 120Combined sources4
Beta strandi128 – 130Combined sources3
Helixi135 – 162Combined sources28

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C02X-ray1.80A/B2-167[»]
1C03X-ray2.30A/B/C/D1-167[»]
1OXBX-ray2.30A2-167[»]
1OXKX-ray2.10A/C/E/G/I/K2-167[»]
1QSPX-ray2.70A/B3-167[»]
2R25X-ray1.70A1-167[»]
5KBXX-ray2.80A1-167[»]
ProteinModelPortaliQ07688.
SMRiQ07688.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07688.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 129HPtPROSITE-ProRule annotationAdd BLAST106

Sequence similaritiesi

Belongs to the YPD1 family.Curated

Phylogenomic databases

HOGENOMiHOG000190539.
InParanoidiQ07688.
KOiK11232.
OMAiWVCERIQ.
OrthoDBiEOG092C5IZ3.

Family and domain databases

CDDicd00088. HPT. 1 hit.
Gene3Di1.20.120.160. 1 hit.
InterProiView protein in InterPro
IPR036641. HPT_dom_sf.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
PfamiView protein in Pfam
PF01627. Hpt. 1 hit.
SMARTiView protein in SMART
SM00073. HPT. 1 hit.
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiView protein in PROSITE
PS50894. HPT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q07688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIPSEIIN WTILNEIISM DDDDSDFSKG LIIQFIDQAQ TTFAQMQRQL
60 70 80 90 100
DGEKNLTELD NLGHFLKGSS AALGLQRIAW VCERIQNLGR KMEHFFPNKT
110 120 130 140 150
ELVNTLSDKS IINGINIDED DEEIKIQVDD KDENSIYLIL IAKALNQSRL
160
EFKLARIELS KYYNTNL
Length:167
Mass (Da):19,169
Last modified:November 1, 1996 - v1
Checksum:i4B923E621C57783D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62016 Genomic DNA. Translation: AAC49440.1.
Z74283 Genomic DNA. Translation: CAA98815.1.
BK006938 Genomic DNA. Translation: DAA11631.1.
PIRiS67799.
RefSeqiNP_010046.1. NM_001180295.1.

Genome annotation databases

EnsemblFungiiYDL235C; YDL235C; YDL235C.
GeneIDi851363.
KEGGisce:YDL235C.

Similar proteinsi

Entry informationi

Entry nameiYPD1_YEAST
AccessioniPrimary (citable) accession number: Q07688
Secondary accession number(s): D6VRC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names