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Q07660 (BRE4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein BRE4
Alternative name(s):
Brefeldin A-sensitivity protein 4
Gene names
Name:BRE4
Ordered Locus Names:YDL231C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length1125 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in intracellular vesicle transport. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Miscellaneous

Deletion of the gene leads to brefeldin A-sensitivity.

Sequence similarities

Belongs to the BRE4 family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendocytosis

Inferred from mutant phenotype PubMed 11378903. Source: SGD

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral to membrane

Inferred from sequence model PubMed 12192589. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11251125Protein BRE4
PRO_0000239634

Regions

Topological domain1 – 143143Cytoplasmic Potential
Transmembrane144 – 16421Helical; Potential
Topological domain165 – 17612Extracellular Potential
Transmembrane177 – 19418Helical; Potential
Topological domain195 – 1984Cytoplasmic Potential
Transmembrane199 – 21921Helical; Potential
Topological domain220 – 2278Extracellular Potential
Transmembrane228 – 24821Helical; Potential
Topological domain249 – 2546Cytoplasmic Potential
Transmembrane255 – 27521Helical; Potential
Topological domain276 – 2849Extracellular Potential
Transmembrane285 – 30521Helical; Potential
Topological domain306 – 706401Cytoplasmic Potential
Transmembrane707 – 72721Helical; Potential
Topological domain728 – 75023Extracellular Potential
Transmembrane751 – 77121Helical; Potential
Topological domain772 – 7809Cytoplasmic Potential
Transmembrane781 – 80121Helical; Potential
Topological domain802 – 84342Extracellular Potential
Transmembrane844 – 86421Helical; Potential
Topological domain865 – 1125261Cytoplasmic Potential

Amino acid modifications

Glycosylation8301N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q07660 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3E0E04F6916A7E74

FASTA1,125129,725
        10         20         30         40         50         60 
MKRYERDRSP TPDPDIVKGS YSQTSLRSLH ELNYKNPAGI SGLSFAGSPQ QSVASLSQMR 

        70         80         90        100        110        120 
LENLVKDKHW EEVEDFGLEE LRDGFFDAAF TKPDSKARSP NSDIDDDNGA ARKKLQSGFT 

       130        140        150        160        170        180 
KLSEYVWTAI YRPIIHFPRD IRKNGVSIFK FFIAYFIAIV ICVIRPSGRW IGHEFRYFLP 

       190        200        210        220        230        240 
IAVLIHHPVR NIGVQLEMTI SSIIGASFGL GWSALAWYIS TATKPTANYQ GGILFQSLTM 

       250        260        270        280        290        300 
ALLFAIWLRS VYRRFFYFTT SFSISIIFTH TVRLASSKFD LKWQIFWDFG ISYLFGLLLS 

       310        320        330        340        350        360 
LLVCVCVSPH SGNAELMEHY NKCLQTTKTF LMALVDTELI KSKEQIYLAQ VKMVKTLNID 

       370        380        390        400        410        420 
LSQGFRDFVN QLTISRFDLQ SLKSLRNSLT AMETSLRVLP IAPKIFNDDE LKKMYEELEK 

       430        440        450        460        470        480 
YRSDSATLSK EASASPQSSG IPTRENTPSA FKPIGPGLLK NEIYINALKA SFSKSIFNLI 

       490        500        510        520        530        540 
LEMIFVLENL SRVLKKYESP NQKNNLDECV KILSHSHSKL KRKIYKLDVC YRDFVNSSFF 

       550        560        570        580        590        600 
SQELLNDEES VDIFLFLRYL RNSARQLVTV IHDCQVLGEN IHWRIALPSY PLSRALTRLP 

       610        620        630        640        650        660 
KQCVLDEGAG NVLHYFEAKR DVDEIFERVY NTYTSRHKYN KGEEEALRLD SQGGDEKSQN 

       670        680        690        700        710        720 
RKNHTISIRA IDHNDFNFHT TQNPWRFKLW KLSRILSGDE CKWTLKITFC MIFLCLPTWL 

       730        740        750        760        770        780 
PESYHWYQEF HCWWAPLTFY LLAHRRYSGN WALVMRRLIC GIVGIFWGWA ANQSRHFGSP 

       790        800        810        820        830        840 
YVVCTFAGLI VVPFSINFLV YRNTKSSFTA LMCFTIIALE PYSKPNRHYN LTTAGIWKST 

       850        860        870        880        890        900 
WVTGLALIIG ILVSIPINWI VWPFRARTEL RDSMSSLLAH LGQSYQTVAD RYLYRDADDA 

       910        920        930        940        950        960 
PTDLTFAFSH IREVRLTQSL EAIRELLKKA RHEPIIISNF NPEKYASLIN SCQFLLSKII 

       970        980        990       1000       1010       1020 
EARISGAFFE IWDQDFDIET TRALLSLRRD SVSSVIFVFY ILSNCFRSKN KIPRYLPNPI 

      1030       1040       1050       1060       1070       1080 
MSRKKLYHFI KKFSEMKDQS HSNLNSGGNS MEKNLFKKIY QQKASSSGQQ QLPLPSVANS 

      1090       1100       1110       1120 
SEIDSEKMHW TEVHGIAFAR AFTDISEALF QVESCAKDIL GEENF

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Identification of yeast deletion strains that are hypersensitive to brefeldin A or monensin, two drugs that affect intracellular transport."
Muren E., Oeyen M., Barmark G., Ronne H.
Yeast 18:163-172(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z74279 Genomic DNA. Translation: CAA98810.1.
BK006938 Genomic DNA. Translation: DAA11635.1.
PIRS67794.
RefSeqNP_010050.1. NM_001180291.1.

3D structure databases

ProteinModelPortalQ07660.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5168N.
MINTMINT-550590.
STRING4932.YDL231C.

Proteomic databases

PaxDbQ07660.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL231C; YDL231C; YDL231C.
GeneID851367.
KEGGsce:YDL231C.

Organism-specific databases

CYGDYDL231c.
SGDS000002390. BRE4.

Phylogenomic databases

eggNOGNOG313447.
HOGENOMHOG000095257.
OMANCFRSKN.
OrthoDBEOG4936S4.

Gene expression databases

GenevestigatorQ07660.
GermOnlineYDL231C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR018820. BRE4-related_DUF2421.
IPR023244. Brefeldin_A-sensitivity_4.
[Graphical view]
PfamPF10334. DUF2421. 1 hit.
[Graphical view]
PRINTSPR02047. BREFELDNASP4.
ProtoNetSearch...

Other

NextBio968483.

Entry information

Entry nameBRE4_YEAST
AccessionPrimary (citable) accession number: Q07660
Secondary accession number(s): D6VRC5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents