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Q07654 (TFF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trefoil factor 3
Alternative name(s):
Intestinal trefoil factor
Short name=hITF
Polypeptide P1.B
Short name=hP1.B
Gene names
Name:TFF3
Synonyms:ITF, TFI
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length80 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). Ref.12

Subunit structure

Monomer. Homodimer; disulfide-linked. Ref.14

Subcellular location

Secretedextracellular spaceextracellular matrixapical lamina Ref.8.

Tissue specificity

Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Ref.1 Ref.8

Sequence similarities

Contains 1 P-type (trefoil) domain.

Sequence caution

The sequence AAH17859.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAL28111.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA95531.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB13731.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q07654-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q07654-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKRVLSCVPEPTVVM
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.10 Ref.11
Chain22 – 8059Trefoil factor 3
PRO_0000023465

Regions

Domain30 – 7344P-type

Amino acid modifications

Disulfide bond32 ↔ 58 By similarity
Disulfide bond42 ↔ 57 By similarity
Disulfide bond52 ↔ 69 By similarity
Disulfide bond78Interchain

Natural variations

Alternative sequence11M → MKRVLSCVPEPTVVM in isoform 2.
VSP_045254

Experimental info

Sequence conflict74 – 763QEA → TRKT in AAA36766. Ref.8

Secondary structure

............. 80
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 8E117A58C0342013

FASTA808,641
        10         20         30         40         50         60 
MAARALCMLG LVLALLSSSS AEEYVGLSAN QCAVPAKDRV DCGYPHVTPK ECNNRGCCFD 

        70         80 
SRIPGVPWCF KPLQEAECTF 

« Hide

Isoform 2 [UniParc].

Checksum: 02276443AEE4CEA5
Show »

FASTA9410,181

References

« Hide 'large scale' references
[1]"hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus."
Hauser F., Poulsom R., Chinery R., Rogers L.A., Hanby A.M., Wright N.A., Hoffmann W.
Proc. Natl. Acad. Sci. U.S.A. 90:6961-6965(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Small intestine.
[2]"The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3."
Seib T., Blin N., Hilgert K., Seifert M., Theisinger B., Engel M., Dooley S., Zang K.D., Welter C.
Genomics 40:200-202(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Refined localization of autosomal recessive nonsyndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure, and exclusion of six known genes in the region."
Berry A., Scott H.S., Kudoh J., Talior I., Korostishevsky M., Wattenhofer M., Guipponi M., Barras C., Rossier C., Shibuya K., Wang J., Kawasaki K., Asakawa S., Minoshima S., Shimizu N., Antonarakis S.E., Bonne-Tamir B.
Genomics 68:22-29(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Transcriptome analysis of human gastric cancer."
Oh J.H., Yang J.O., Hahn Y., Kim M.R., Byun S.S., Jeon Y.J., Kim J.M., Song K.S., Noh S.M., Kim S., Yoo H.S., Kim Y.S., Kim N.S.
Mamm. Genome 16:942-954(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]"The DNA sequence of human chromosome 21."
Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. expand/collapse author list , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[7]"mRNA 5' region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs."
Casadei R., Strippoli P., D'Addabbo P., Canaider S., Lenzi L., Vitale L., Giannone S., Frabetti F., Facchin F., Carinci P., Zannotti M.
Gene 321:185-193(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-51 (ISOFORM 1).
Tissue: Heart.
[8]"Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion."
Podolsky D.K., Lynch-Devaney K., Stow J.L., Oates P., Murgue B., Debeaumont M., Sands B.E., Mahida Y.R.
J. Biol. Chem. 268:6694-6702(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-80 (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Colon.
[9]Erratum
Podolsky D.K., Lynch-Devaney K., Stow J.L., Oates P., Murgue B., De-Beaumont M., Sands B.E., Mahida Y.R.
J. Biol. Chem. 268:12230-12230(1993)
[10]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-36.
[11]"Comprehensive proteomic analysis of human endometrial fluid aspirate."
Casado-Vela J., Rodriguez-Suarez E., Iloro I., Ametzazurra A., Alkorta N., Garcia-Velasco J.A., Matorras R., Prieto B., Gonzalez S., Nagore D., Simon L., Elortza F.
J. Proteome Res. 8:4622-4632(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-37, IDENTIFICATION BY MASS SPECTROMETRY.
[12]"Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor."
Oertel M., Graness A., Thim L., Buhling F., Kalbacher H., Hoffmann W.
Am. J. Respir. Cell Mol. Biol. 25:418-424(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Incorporation of mannose and glucose into prenylphosphate sugars in isolated human platelet membranes."
De Luca S.
Biochim. Biophys. Acta 498:341-348(1977) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 22-80.
[14]"Solution structure of the disulfide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins."
Muskett F.W., May F.E., Westley B.R., Feeney J.
Biochemistry 42:15139-15147(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 22-80, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L15203 mRNA. Translation: AAA59981.1.
U25657, U25654, U25656 Genomic DNA. Translation: AAA83628.1.
AB038162 Genomic DNA. Translation: BAB13731.1. Different initiation.
BM743413 mRNA. No translation available.
AP001746 Genomic DNA. Translation: BAA95531.1. Different initiation.
BC017859 mRNA. Translation: AAH17859.1. Different initiation.
AF432265 mRNA. Translation: AAL28111.1. Different initiation.
L08044 mRNA. Translation: AAA36766.1.
CCDSCCDS33565.2. [Q07654-2]
PIRA48284.
RefSeqNP_003217.3. NM_003226.3. [Q07654-2]
UniGeneHs.82961.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1E9TNMR-A22-80[»]
1PE3NMR-1/222-80[»]
ProteinModelPortalQ07654.
SMRQ07654. Positions 22-80.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112891. 1 interaction.
STRING9606.ENSP00000291525.

Polymorphism databases

DMDM585328.

Proteomic databases

PaxDbQ07654.
PRIDEQ07654.

Protocols and materials databases

DNASU7033.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000518498; ENSP00000430690; ENSG00000160180. [Q07654-2]
GeneID7033.
KEGGhsa:7033.

Organism-specific databases

CTD7033.
GeneCardsGC21M043726.
H-InvDBHIX0016137.
HGNCHGNC:11757. TFF3.
HPACAB020681.
HPA035464.
MIM600633. gene.
neXtProtNX_Q07654.
PharmGKBPA36472.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG79314.
HOGENOMHOG000121778.
HOVERGENHBG004364.
OrthoDBEOG70W3GT.
PhylomeDBQ07654.
TreeFamTF336092.

Gene expression databases

BgeeQ07654.
CleanExHS_TFF3.
GenevestigatorQ07654.

Family and domain databases

Gene3D4.10.110.10. 1 hit.
InterProIPR000519. P_trefoil.
IPR017994. P_trefoil_chordata.
IPR017957. P_trefoil_CS.
[Graphical view]
PfamPF00088. Trefoil. 1 hit.
[Graphical view]
PRINTSPR00680. PTREFOIL.
SMARTSM00018. PD. 1 hit.
[Graphical view]
SUPFAMSSF57492. SSF57492. 1 hit.
PROSITEPS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ07654.
GeneWikiTrefoil_factor_3.
GenomeRNAi7033.
NextBio27477.
PROQ07654.
SOURCESearch...

Entry information

Entry nameTFF3_HUMAN
AccessionPrimary (citable) accession number: Q07654
Secondary accession number(s): E9PBB5, Q96NX0, Q9UDA5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM