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Q07654

- TFF3_HUMAN

UniProt

Q07654 - TFF3_HUMAN

Protein

Trefoil factor 3

Gene

TFF3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).1 Publication

    GO - Biological processi

    1. defense response Source: ProtInc
    2. digestion Source: ProtInc
    3. response to peptide hormone Source: Ensembl

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trefoil factor 3
    Alternative name(s):
    Intestinal trefoil factor
    Short name:
    hITF
    Polypeptide P1.B
    Short name:
    hP1.B
    Gene namesi
    Name:TFF3
    Synonyms:ITF, TFI
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 21

    Organism-specific databases

    HGNCiHGNC:11757. TFF3.

    Subcellular locationi

    Secretedextracellular spaceextracellular matrixapical lamina 1 Publication

    GO - Cellular componenti

    1. apical lamina of hyaline layer Source: UniProtKB-SubCell
    2. extracellular region Source: ProtInc
    3. extracellular vesicular exosome Source: UniProt
    4. secretory granule Source: Ensembl

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36472.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21212 PublicationsAdd
    BLAST
    Chaini22 – 8059Trefoil factor 3PRO_0000023465Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 58PROSITE-ProRule annotation
    Disulfide bondi42 ↔ 57PROSITE-ProRule annotation
    Disulfide bondi52 ↔ 69PROSITE-ProRule annotation
    Disulfide bondi78 – 78Interchain

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ07654.
    PRIDEiQ07654.

    Expressioni

    Tissue specificityi

    Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.2 Publications

    Gene expression databases

    BgeeiQ07654.
    CleanExiHS_TFF3.
    GenevestigatoriQ07654.

    Organism-specific databases

    HPAiCAB020681.
    HPA035464.

    Interactioni

    Subunit structurei

    Monomer. Homodimer; disulfide-linked.1 Publication

    Protein-protein interaction databases

    BioGridi112891. 1 interaction.
    STRINGi9606.ENSP00000291525.

    Structurei

    Secondary structure

    1
    80
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 323
    Beta strandi36 – 394
    Turni49 – 546
    Beta strandi55 – 584
    Beta strandi68 – 703
    Helixi74 – 763
    Turni77 – 793

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E9TNMR-A22-80[»]
    1PE3NMR-1/222-80[»]
    ProteinModelPortaliQ07654.
    SMRiQ07654. Positions 22-80.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ07654.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 7344P-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 P-type (trefoil) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG79314.
    HOGENOMiHOG000121778.
    HOVERGENiHBG004364.
    OrthoDBiEOG70W3GT.
    PhylomeDBiQ07654.
    TreeFamiTF336092.

    Family and domain databases

    Gene3Di4.10.110.10. 1 hit.
    InterProiIPR000519. P_trefoil.
    IPR017994. P_trefoil_chordata.
    IPR017957. P_trefoil_CS.
    [Graphical view]
    PfamiPF00088. Trefoil. 1 hit.
    [Graphical view]
    PRINTSiPR00680. PTREFOIL.
    SMARTiSM00018. PD. 1 hit.
    [Graphical view]
    SUPFAMiSSF57492. SSF57492. 1 hit.
    PROSITEiPS00025. P_TREFOIL_1. 1 hit.
    PS51448. P_TREFOIL_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q07654-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAARALCMLG LVLALLSSSS AEEYVGLSAN QCAVPAKDRV DCGYPHVTPK   50
    ECNNRGCCFD SRIPGVPWCF KPLQEAECTF 80
    Length:80
    Mass (Da):8,641
    Last modified:October 1, 1994 - v1
    Checksum:i8E117A58C0342013
    GO
    Isoform 2 (identifier: Q07654-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MKRVLSCVPEPTVVM

    Note: No experimental confirmation available.

    Show »
    Length:94
    Mass (Da):10,181
    Checksum:i02276443AEE4CEA5
    GO

    Sequence cautioni

    The sequence AAH17859.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAL28111.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAA95531.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAB13731.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 763QEA → TRKT in AAA36766. (PubMed:8454642)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MKRVLSCVPEPTVVM in isoform 2. 1 PublicationVSP_045254

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15203 mRNA. Translation: AAA59981.1.
    U25657, U25654, U25656 Genomic DNA. Translation: AAA83628.1.
    AB038162 Genomic DNA. Translation: BAB13731.1. Different initiation.
    BM743413 mRNA. No translation available.
    AP001746 Genomic DNA. Translation: BAA95531.1. Different initiation.
    BC017859 mRNA. Translation: AAH17859.1. Different initiation.
    AF432265 mRNA. Translation: AAL28111.1. Different initiation.
    L08044 mRNA. Translation: AAA36766.1.
    CCDSiCCDS33565.2. [Q07654-2]
    PIRiA48284.
    RefSeqiNP_003217.3. NM_003226.3. [Q07654-2]
    UniGeneiHs.82961.

    Genome annotation databases

    EnsembliENST00000518498; ENSP00000430690; ENSG00000160180. [Q07654-2]
    GeneIDi7033.
    KEGGihsa:7033.

    Polymorphism databases

    DMDMi585328.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15203 mRNA. Translation: AAA59981.1 .
    U25657 , U25654 , U25656 Genomic DNA. Translation: AAA83628.1 .
    AB038162 Genomic DNA. Translation: BAB13731.1 . Different initiation.
    BM743413 mRNA. No translation available.
    AP001746 Genomic DNA. Translation: BAA95531.1 . Different initiation.
    BC017859 mRNA. Translation: AAH17859.1 . Different initiation.
    AF432265 mRNA. Translation: AAL28111.1 . Different initiation.
    L08044 mRNA. Translation: AAA36766.1 .
    CCDSi CCDS33565.2. [Q07654-2 ]
    PIRi A48284.
    RefSeqi NP_003217.3. NM_003226.3. [Q07654-2 ]
    UniGenei Hs.82961.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E9T NMR - A 22-80 [» ]
    1PE3 NMR - 1/2 22-80 [» ]
    ProteinModelPortali Q07654.
    SMRi Q07654. Positions 22-80.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112891. 1 interaction.
    STRINGi 9606.ENSP00000291525.

    Polymorphism databases

    DMDMi 585328.

    Proteomic databases

    PaxDbi Q07654.
    PRIDEi Q07654.

    Protocols and materials databases

    DNASUi 7033.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000518498 ; ENSP00000430690 ; ENSG00000160180 . [Q07654-2 ]
    GeneIDi 7033.
    KEGGi hsa:7033.

    Organism-specific databases

    CTDi 7033.
    GeneCardsi GC21M043726.
    H-InvDB HIX0016137.
    HGNCi HGNC:11757. TFF3.
    HPAi CAB020681.
    HPA035464.
    MIMi 600633. gene.
    neXtProti NX_Q07654.
    PharmGKBi PA36472.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG79314.
    HOGENOMi HOG000121778.
    HOVERGENi HBG004364.
    OrthoDBi EOG70W3GT.
    PhylomeDBi Q07654.
    TreeFami TF336092.

    Miscellaneous databases

    EvolutionaryTracei Q07654.
    GeneWikii Trefoil_factor_3.
    GenomeRNAii 7033.
    NextBioi 27477.
    PROi Q07654.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q07654.
    CleanExi HS_TFF3.
    Genevestigatori Q07654.

    Family and domain databases

    Gene3Di 4.10.110.10. 1 hit.
    InterProi IPR000519. P_trefoil.
    IPR017994. P_trefoil_chordata.
    IPR017957. P_trefoil_CS.
    [Graphical view ]
    Pfami PF00088. Trefoil. 1 hit.
    [Graphical view ]
    PRINTSi PR00680. PTREFOIL.
    SMARTi SM00018. PD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57492. SSF57492. 1 hit.
    PROSITEi PS00025. P_TREFOIL_1. 1 hit.
    PS51448. P_TREFOIL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus."
      Hauser F., Poulsom R., Chinery R., Rogers L.A., Hanby A.M., Wright N.A., Hoffmann W.
      Proc. Natl. Acad. Sci. U.S.A. 90:6961-6965(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Tissue: Small intestine.
    2. "The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3."
      Seib T., Blin N., Hilgert K., Seifert M., Theisinger B., Engel M., Dooley S., Zang K.D., Welter C.
      Genomics 40:200-202(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Refined localization of autosomal recessive nonsyndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure, and exclusion of six known genes in the region."
      Berry A., Scott H.S., Kudoh J., Talior I., Korostishevsky M., Wattenhofer M., Guipponi M., Barras C., Rossier C., Shibuya K., Wang J., Kawasaki K., Asakawa S., Minoshima S., Shimizu N., Antonarakis S.E., Bonne-Tamir B.
      Genomics 68:22-29(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    5. "The DNA sequence of human chromosome 21."
      Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
      , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
      Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    7. "mRNA 5' region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs."
      Casadei R., Strippoli P., D'Addabbo P., Canaider S., Lenzi L., Vitale L., Giannone S., Frabetti F., Facchin F., Carinci P., Zannotti M.
      Gene 321:185-193(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-51 (ISOFORM 1).
      Tissue: Heart.
    8. "Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion."
      Podolsky D.K., Lynch-Devaney K., Stow J.L., Oates P., Murgue B., Debeaumont M., Sands B.E., Mahida Y.R.
      J. Biol. Chem. 268:6694-6702(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-80 (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Colon.
    9. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z., Henzel W.J.
      Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-36.
    10. Cited for: PROTEIN SEQUENCE OF 22-37, IDENTIFICATION BY MASS SPECTROMETRY.
    11. "Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor."
      Oertel M., Graness A., Thim L., Buhling F., Kalbacher H., Hoffmann W.
      Am. J. Respir. Cell Mol. Biol. 25:418-424(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Incorporation of mannose and glucose into prenylphosphate sugars in isolated human platelet membranes."
      De Luca S.
      Biochim. Biophys. Acta 498:341-348(1977) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 22-80.
    13. "Solution structure of the disulfide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins."
      Muskett F.W., May F.E., Westley B.R., Feeney J.
      Biochemistry 42:15139-15147(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 22-80, SUBUNIT.

    Entry informationi

    Entry nameiTFF3_HUMAN
    AccessioniPrimary (citable) accession number: Q07654
    Secondary accession number(s): E9PBB5, Q96NX0, Q9UDA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 21
      Human chromosome 21: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3