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Q07654

- TFF3_HUMAN

UniProt

Q07654 - TFF3_HUMAN

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Protein

Trefoil factor 3

Gene
TFF3, ITF, TFI
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).1 Publication

GO - Biological processi

  1. defense response Source: ProtInc
  2. digestion Source: ProtInc
  3. response to peptide hormone Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Trefoil factor 3
Alternative name(s):
Intestinal trefoil factor
Short name:
hITF
Polypeptide P1.B
Short name:
hP1.B
Gene namesi
Name:TFF3
Synonyms:ITF, TFI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 21

Organism-specific databases

HGNCiHGNC:11757. TFF3.

Subcellular locationi

Secretedextracellular spaceextracellular matrixapical lamina 1 Publication

GO - Cellular componenti

  1. apical lamina of hyaline layer Source: UniProtKB-SubCell
  2. extracellular region Source: ProtInc
  3. extracellular vesicular exosome Source: UniProt
  4. secretory granule Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36472.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21212 PublicationsAdd
BLAST
Chaini22 – 8059Trefoil factor 3PRO_0000023465Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 58 By similarity
Disulfide bondi42 ↔ 57 By similarity
Disulfide bondi52 ↔ 69 By similarity
Disulfide bondi78 – 78Interchain

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ07654.
PRIDEiQ07654.

Expressioni

Tissue specificityi

Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.2 Publications

Gene expression databases

BgeeiQ07654.
CleanExiHS_TFF3.
GenevestigatoriQ07654.

Organism-specific databases

HPAiCAB020681.
HPA035464.

Interactioni

Subunit structurei

Monomer. Homodimer; disulfide-linked.1 Publication

Protein-protein interaction databases

BioGridi112891. 1 interaction.
STRINGi9606.ENSP00000291525.

Structurei

Secondary structure

1
80
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 323
Beta strandi36 – 394
Turni49 – 546
Beta strandi55 – 584
Beta strandi68 – 703
Helixi74 – 763
Turni77 – 793

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E9TNMR-A22-80[»]
1PE3NMR-1/222-80[»]
ProteinModelPortaliQ07654.
SMRiQ07654. Positions 22-80.

Miscellaneous databases

EvolutionaryTraceiQ07654.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 7344P-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG79314.
HOGENOMiHOG000121778.
HOVERGENiHBG004364.
OrthoDBiEOG70W3GT.
PhylomeDBiQ07654.
TreeFamiTF336092.

Family and domain databases

Gene3Di4.10.110.10. 1 hit.
InterProiIPR000519. P_trefoil.
IPR017994. P_trefoil_chordata.
IPR017957. P_trefoil_CS.
[Graphical view]
PfamiPF00088. Trefoil. 1 hit.
[Graphical view]
PRINTSiPR00680. PTREFOIL.
SMARTiSM00018. PD. 1 hit.
[Graphical view]
SUPFAMiSSF57492. SSF57492. 1 hit.
PROSITEiPS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q07654-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAARALCMLG LVLALLSSSS AEEYVGLSAN QCAVPAKDRV DCGYPHVTPK   50
ECNNRGCCFD SRIPGVPWCF KPLQEAECTF 80
Length:80
Mass (Da):8,641
Last modified:October 1, 1994 - v1
Checksum:i8E117A58C0342013
GO
Isoform 2 (identifier: Q07654-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKRVLSCVPEPTVVM

Note: No experimental confirmation available.

Show »
Length:94
Mass (Da):10,181
Checksum:i02276443AEE4CEA5
GO

Sequence cautioni

The sequence AAH17859.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAL28111.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAA95531.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB13731.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MKRVLSCVPEPTVVM in isoform 2. VSP_045254

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 763QEA → TRKT in AAA36766. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L15203 mRNA. Translation: AAA59981.1.
U25657, U25654, U25656 Genomic DNA. Translation: AAA83628.1.
AB038162 Genomic DNA. Translation: BAB13731.1. Different initiation.
BM743413 mRNA. No translation available.
AP001746 Genomic DNA. Translation: BAA95531.1. Different initiation.
BC017859 mRNA. Translation: AAH17859.1. Different initiation.
AF432265 mRNA. Translation: AAL28111.1. Different initiation.
L08044 mRNA. Translation: AAA36766.1.
CCDSiCCDS33565.2. [Q07654-2]
PIRiA48284.
RefSeqiNP_003217.3. NM_003226.3. [Q07654-2]
UniGeneiHs.82961.

Genome annotation databases

EnsembliENST00000518498; ENSP00000430690; ENSG00000160180. [Q07654-2]
GeneIDi7033.
KEGGihsa:7033.

Polymorphism databases

DMDMi585328.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L15203 mRNA. Translation: AAA59981.1 .
U25657 , U25654 , U25656 Genomic DNA. Translation: AAA83628.1 .
AB038162 Genomic DNA. Translation: BAB13731.1 . Different initiation.
BM743413 mRNA. No translation available.
AP001746 Genomic DNA. Translation: BAA95531.1 . Different initiation.
BC017859 mRNA. Translation: AAH17859.1 . Different initiation.
AF432265 mRNA. Translation: AAL28111.1 . Different initiation.
L08044 mRNA. Translation: AAA36766.1 .
CCDSi CCDS33565.2. [Q07654-2 ]
PIRi A48284.
RefSeqi NP_003217.3. NM_003226.3. [Q07654-2 ]
UniGenei Hs.82961.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E9T NMR - A 22-80 [» ]
1PE3 NMR - 1/2 22-80 [» ]
ProteinModelPortali Q07654.
SMRi Q07654. Positions 22-80.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112891. 1 interaction.
STRINGi 9606.ENSP00000291525.

Polymorphism databases

DMDMi 585328.

Proteomic databases

PaxDbi Q07654.
PRIDEi Q07654.

Protocols and materials databases

DNASUi 7033.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000518498 ; ENSP00000430690 ; ENSG00000160180 . [Q07654-2 ]
GeneIDi 7033.
KEGGi hsa:7033.

Organism-specific databases

CTDi 7033.
GeneCardsi GC21M043726.
H-InvDB HIX0016137.
HGNCi HGNC:11757. TFF3.
HPAi CAB020681.
HPA035464.
MIMi 600633. gene.
neXtProti NX_Q07654.
PharmGKBi PA36472.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG79314.
HOGENOMi HOG000121778.
HOVERGENi HBG004364.
OrthoDBi EOG70W3GT.
PhylomeDBi Q07654.
TreeFami TF336092.

Miscellaneous databases

EvolutionaryTracei Q07654.
GeneWikii Trefoil_factor_3.
GenomeRNAii 7033.
NextBioi 27477.
PROi Q07654.
SOURCEi Search...

Gene expression databases

Bgeei Q07654.
CleanExi HS_TFF3.
Genevestigatori Q07654.

Family and domain databases

Gene3Di 4.10.110.10. 1 hit.
InterProi IPR000519. P_trefoil.
IPR017994. P_trefoil_chordata.
IPR017957. P_trefoil_CS.
[Graphical view ]
Pfami PF00088. Trefoil. 1 hit.
[Graphical view ]
PRINTSi PR00680. PTREFOIL.
SMARTi SM00018. PD. 1 hit.
[Graphical view ]
SUPFAMi SSF57492. SSF57492. 1 hit.
PROSITEi PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus."
    Hauser F., Poulsom R., Chinery R., Rogers L.A., Hanby A.M., Wright N.A., Hoffmann W.
    Proc. Natl. Acad. Sci. U.S.A. 90:6961-6965(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Small intestine.
  2. "The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3."
    Seib T., Blin N., Hilgert K., Seifert M., Theisinger B., Engel M., Dooley S., Zang K.D., Welter C.
    Genomics 40:200-202(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Refined localization of autosomal recessive nonsyndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure, and exclusion of six known genes in the region."
    Berry A., Scott H.S., Kudoh J., Talior I., Korostishevsky M., Wattenhofer M., Guipponi M., Barras C., Rossier C., Shibuya K., Wang J., Kawasaki K., Asakawa S., Minoshima S., Shimizu N., Antonarakis S.E., Bonne-Tamir B.
    Genomics 68:22-29(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "The DNA sequence of human chromosome 21."
    Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
    , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
    Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  7. "mRNA 5' region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs."
    Casadei R., Strippoli P., D'Addabbo P., Canaider S., Lenzi L., Vitale L., Giannone S., Frabetti F., Facchin F., Carinci P., Zannotti M.
    Gene 321:185-193(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-51 (ISOFORM 1).
    Tissue: Heart.
  8. "Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion."
    Podolsky D.K., Lynch-Devaney K., Stow J.L., Oates P., Murgue B., Debeaumont M., Sands B.E., Mahida Y.R.
    J. Biol. Chem. 268:6694-6702(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-80 (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Colon.
  9. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-36.
  10. Cited for: PROTEIN SEQUENCE OF 22-37, IDENTIFICATION BY MASS SPECTROMETRY.
  11. "Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor."
    Oertel M., Graness A., Thim L., Buhling F., Kalbacher H., Hoffmann W.
    Am. J. Respir. Cell Mol. Biol. 25:418-424(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Incorporation of mannose and glucose into prenylphosphate sugars in isolated human platelet membranes."
    De Luca S.
    Biochim. Biophys. Acta 498:341-348(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 22-80.
  13. "Solution structure of the disulfide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins."
    Muskett F.W., May F.E., Westley B.R., Feeney J.
    Biochemistry 42:15139-15147(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 22-80, SUBUNIT.

Entry informationi

Entry nameiTFF3_HUMAN
AccessioniPrimary (citable) accession number: Q07654
Secondary accession number(s): E9PBB5, Q96NX0, Q9UDA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 3, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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