Q07654 (TFF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trefoil factor 3

Alternative name(s):
Intestinal trefoil factor
Short name=hITF
Polypeptide P1.B
Short name=hP1.B

Gene names

Name:	TFF3
Synonyms:	ITF, TFI

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	80 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). Ref.10
Subunit structure	Monomer. Homodimer; disulfide-linked. Ref.12
Subcellular location	Secreted › extracellular space › extracellular matrix › apical lamina Ref.7.
Tissue specificity	Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Ref.1 Ref.7
Sequence similarities	Contains 1 P-type (trefoil) domain.
Sequence caution	The sequence AAH17859.1 differs from that shown. Reason: Erroneous initiation. The sequence AAL28111.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA95531.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB13731.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	defense response Traceable author statement. Source: ProtInc digestion Traceable author statement. Source: ProtInc
Cellular component	apical lamina of hyaline layer Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Ref.9

Chain

22 – 80

59

Trefoil factor 3

PRO_0000023465

Regions

Domain

30 – 73

44

P-type

Amino acid modifications

Disulfide bond

32 ↔ 58

By similarity

Disulfide bond

42 ↔ 57

By similarity

Disulfide bond

52 ↔ 69

By similarity

Disulfide bond

78

Interchain

Experimental info

Sequence conflict

74 – 76

3

QEA → TRKT in AAA36766. Ref.7

Secondary structure

1

.

.

.

.

.

.

.

.

80

Helix Strand Turn

Details...

Helix

30 – 32

3

Turn

49 – 54

6

Beta strand

55 – 58

4

Beta strand

68 – 70

3

Sequences

	Sequence		Length	Mass (Da)
	Q07654 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 8E117A58C0342013	FASTA	80	8,641
	10 20 30 40 50 60 MAARALCMLG LVLALLSSSS AEEYVGLSAN QCAVPAKDRV DCGYPHVTPK ECNNRGCCFD 70 80 SRIPGVPWCF KPLQEAECTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus." Hauser F., Poulsom R., Chinery R., Rogers L.A., Hanby A.M., Wright N.A., Hoffmann W. Proc. Natl. Acad. Sci. U.S.A. 90:6961-6965(1993) [PubMed: 8346203] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Small intestine.
[2]	"The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3." Seib T., Blin N., Hilgert K., Seifert M., Theisinger B., Engel M., Dooley S., Zang K.D., Welter C. Genomics 40:200-202(1997) [PubMed: 9070946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Refined localization of autosomal recessive nonsyndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure, and exclusion of six known genes in the region." Berry A., Scott H.S., Kudoh J., Talior I., Korostishevsky M., Wattenhofer M., Guipponi M., Barras C., Rossier C., Shibuya K., Wang J., Kawasaki K., Asakawa S., Minoshima S., Shimizu N., Antonarakis S.E., Bonne-Tamir B. Genomics 68:22-29(2000) [PubMed: 10950923] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	"mRNA 5' region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs." Casadei R., Strippoli P., D'Addabbo P., Canaider S., Lenzi L., Vitale L., Giannone S., Frabetti F., Facchin F., Carinci P., Zannotti M. Gene 321:185-193(2003) [PubMed: 14637006] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-51. Tissue: Heart.
[7]	"Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion." Podolsky D.K., Lynch-Devaney K., Stow J.L., Oates P., Murgue B., Debeaumont M., Sands B.E., Mahida Y.R. J. Biol. Chem. 268:6694-6702(1993) [PubMed: 8454642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-80, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Colon.
[8]	Erratum Podolsky D.K., Lynch-Devaney K., Stow J.L., Oates P., Murgue B., De-Beaumont M., Sands B.E., Mahida Y.R. J. Biol. Chem. 268:12230-12230(1993)
[9]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-36.
[10]	"Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor." Oertel M., Graness A., Thim L., Buhling F., Kalbacher H., Hoffmann W. Am. J. Respir. Cell Mol. Biol. 25:418-424(2001) [PubMed: 11694446] [Abstract] Cited for: FUNCTION.
[11]	"Incorporation of mannose and glucose into prenylphosphate sugars in isolated human platelet membranes." De Luca S. Biochim. Biophys. Acta 498:341-348(1977) [PubMed: 18209] [Abstract] Cited for: STRUCTURE BY NMR OF 22-80.
[12]	"Solution structure of the disulfide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins." Muskett F.W., May F.E., Westley B.R., Feeney J. Biochemistry 42:15139-15147(2003) [PubMed: 14690424] [Abstract] Cited for: STRUCTURE BY NMR OF 22-80, SUBUNIT.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L15203 mRNA. Translation: AAA59981.1.
U25657, U25654, U25656 Genomic DNA. Translation: AAA83628.1.
AB038162 Genomic DNA. Translation: BAB13731.1. Different initiation.
AP001746 Genomic DNA. Translation: BAA95531.1. Different initiation.
BC017859 mRNA. Translation: AAH17859.1. Different initiation.
AF432265 mRNA. Translation: AAL28111.1. Different initiation.
L08044 mRNA. Translation: AAA36766.1.

IPI

IPI00018909.

PIR

A48284.

RefSeq

NP_003217.3. NM_003226.3.

UniGene

Hs.82961.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E9T	NMR	-	A	22-80	[»]
1PE3	NMR	-	1/2	22-80	[»]

ProteinModelPortal

Q07654.

SMR

Q07654. Positions 22-80.

ModBase

Search...

Protein-protein interaction databases

STRING

Q07654.

Polymorphism databases

DMDM

585328.

Proteomic databases

PRIDE

Q07654.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000291525; ENSP00000291525; ENSG00000160180.
ENST00000414929; ENSP00000392810; ENSG00000160180.

GeneID

7033.

KEGG

hsa:7033.

UCSC

uc002zav.1. human.

Organism-specific databases

CTD

7033.

GeneCards

GC21M043726.

H-InvDB

HIX0016137.

HGNC

HGNC:11757. TFF3.

HPA

CAB020681.

MIM

600633. gene.

neXtProt

NX_Q07654.

PharmGKB

PA36472.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21468.

GeneTree

ENSGT00390000000115.

HOVERGEN

HBG004364.

OrthoDB

EOG4907BN.

Enzyme and pathway databases

Pathway_Interaction_DB

hif1_tfpathway. HIF-1-alpha transcription factor network.
il4_2pathway. IL4-mediated signaling events.

Gene expression databases

ArrayExpress

Q07654.

Bgee

Q07654.

CleanEx

HS_TFF3.

Genevestigator

Q07654.

GermOnline

ENSG00000160180. Homo sapiens.

Family and domain databases

InterPro

IPR000519. P_trefoil.
IPR017994. P_trefoil_chordata.
IPR017957. P_trefoil_CS.
[Graphical view]

Gene3D

G3DSA:4.10.110.10. P_trefoil. 1 hit.

Pfam

PF00088. Trefoil. 1 hit.
[Graphical view]

PRINTS

PR00680. PTREFOIL.

SMART

SM00018. PD. 1 hit.
[Graphical view]

SUPFAM

SSF57492. P_trefoil. 1 hit.

PROSITE

PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

TFF3_HUMAN

Accession

Primary (citable) accession number: Q07654
Secondary accession number(s): Q96NX0, Q9UDA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.