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Protein

Trefoil factor 3

Gene

TFF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).1 Publication

GO - Biological processi

  1. defense response Source: ProtInc
  2. digestion Source: ProtInc
  3. response to peptide hormone Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Trefoil factor 3
Alternative name(s):
Intestinal trefoil factor
Short name:
hITF
Polypeptide P1.B
Short name:
hP1.B
Gene namesi
Name:TFF3
Synonyms:ITF, TFI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:11757. TFF3.

Subcellular locationi

Secretedextracellular spaceextracellular matrix 1 Publication. Cytoplasm 2 Publications

GO - Cellular componenti

  1. extracellular region Source: ProtInc
  2. extracellular vesicular exosome Source: UniProtKB
  3. proteinaceous extracellular matrix Source: UniProtKB-SubCell
  4. secretory granule Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36472.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21212 PublicationsAdd
BLAST
Chaini22 – 8059Trefoil factor 3PRO_0000023465Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 58PROSITE-ProRule annotation
Disulfide bondi42 ↔ 57PROSITE-ProRule annotation
Disulfide bondi52 ↔ 69PROSITE-ProRule annotation
Disulfide bondi78 – 78Interchain

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ07654.
PRIDEiQ07654.

Expressioni

Tissue specificityi

Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular and supraoptic nuclei (at protein level).2 Publications

Gene expression databases

BgeeiQ07654.
CleanExiHS_TFF3.
ExpressionAtlasiQ07654. baseline and differential.
GenevestigatoriQ07654.

Organism-specific databases

HPAiCAB020681.
HPA035464.

Interactioni

Subunit structurei

Monomer. Homodimer; disulfide-linked.1 Publication

Protein-protein interaction databases

BioGridi112891. 3 interactions.
STRINGi9606.ENSP00000291525.

Structurei

Secondary structure

1
80
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 323Combined sources
Beta strandi36 – 394Combined sources
Turni49 – 546Combined sources
Beta strandi55 – 584Combined sources
Beta strandi68 – 703Combined sources
Helixi74 – 763Combined sources
Turni77 – 793Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E9TNMR-A22-80[»]
1PE3NMR-1/222-80[»]
ProteinModelPortaliQ07654.
SMRiQ07654. Positions 22-80.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07654.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 7344P-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 P-type (trefoil) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG79314.
HOGENOMiHOG000121778.
HOVERGENiHBG004364.
InParanoidiQ07654.
OrthoDBiEOG70W3GT.
PhylomeDBiQ07654.
TreeFamiTF336092.

Family and domain databases

Gene3Di4.10.110.10. 1 hit.
InterProiIPR000519. P_trefoil.
IPR017994. P_trefoil_chordata.
IPR017957. P_trefoil_CS.
[Graphical view]
PfamiPF00088. Trefoil. 1 hit.
[Graphical view]
PRINTSiPR00680. PTREFOIL.
SMARTiSM00018. PD. 1 hit.
[Graphical view]
SUPFAMiSSF57492. SSF57492. 1 hit.
PROSITEiPS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARALCMLG LVLALLSSSS AEEYVGLSAN QCAVPAKDRV DCGYPHVTPK
60 70 80
ECNNRGCCFD SRIPGVPWCF KPLQEAECTF
Length:80
Mass (Da):8,641
Last modified:September 30, 1994 - v1
Checksum:i8E117A58C0342013
GO

Sequence cautioni

The sequence AAH17859.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAL28111.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA95531.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB13731.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 763QEA → TRKT in AAA36766 (PubMed:8454642).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15203 mRNA. Translation: AAA59981.1.
U25657, U25654, U25656 Genomic DNA. Translation: AAA83628.1.
AB038162 Genomic DNA. Translation: BAB13731.1. Different initiation.
AP001746 Genomic DNA. Translation: BAA95531.1. Different initiation.
BC017859 mRNA. Translation: AAH17859.1. Different initiation.
AF432265 mRNA. Translation: AAL28111.1. Different initiation.
L08044 mRNA. Translation: AAA36766.1.
PIRiA48284.
RefSeqiNP_003217.3. NM_003226.3.
UniGeneiHs.82961.

Genome annotation databases

GeneIDi7033.
KEGGihsa:7033.
UCSCiuc002zav.3. human.

Polymorphism databases

DMDMi585328.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15203 mRNA. Translation: AAA59981.1.
U25657, U25654, U25656 Genomic DNA. Translation: AAA83628.1.
AB038162 Genomic DNA. Translation: BAB13731.1. Different initiation.
AP001746 Genomic DNA. Translation: BAA95531.1. Different initiation.
BC017859 mRNA. Translation: AAH17859.1. Different initiation.
AF432265 mRNA. Translation: AAL28111.1. Different initiation.
L08044 mRNA. Translation: AAA36766.1.
PIRiA48284.
RefSeqiNP_003217.3. NM_003226.3.
UniGeneiHs.82961.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E9TNMR-A22-80[»]
1PE3NMR-1/222-80[»]
ProteinModelPortaliQ07654.
SMRiQ07654. Positions 22-80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112891. 3 interactions.
STRINGi9606.ENSP00000291525.

Polymorphism databases

DMDMi585328.

Proteomic databases

PaxDbiQ07654.
PRIDEiQ07654.

Protocols and materials databases

DNASUi7033.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7033.
KEGGihsa:7033.
UCSCiuc002zav.3. human.

Organism-specific databases

CTDi7033.
GeneCardsiGC21M043726.
H-InvDBHIX0016137.
HGNCiHGNC:11757. TFF3.
HPAiCAB020681.
HPA035464.
MIMi600633. gene.
neXtProtiNX_Q07654.
PharmGKBiPA36472.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG79314.
HOGENOMiHOG000121778.
HOVERGENiHBG004364.
InParanoidiQ07654.
OrthoDBiEOG70W3GT.
PhylomeDBiQ07654.
TreeFamiTF336092.

Miscellaneous databases

ChiTaRSiTFF3. human.
EvolutionaryTraceiQ07654.
GeneWikiiTrefoil_factor_3.
GenomeRNAii7033.
NextBioi27477.
PROiQ07654.
SOURCEiSearch...

Gene expression databases

BgeeiQ07654.
CleanExiHS_TFF3.
ExpressionAtlasiQ07654. baseline and differential.
GenevestigatoriQ07654.

Family and domain databases

Gene3Di4.10.110.10. 1 hit.
InterProiIPR000519. P_trefoil.
IPR017994. P_trefoil_chordata.
IPR017957. P_trefoil_CS.
[Graphical view]
PfamiPF00088. Trefoil. 1 hit.
[Graphical view]
PRINTSiPR00680. PTREFOIL.
SMARTiSM00018. PD. 1 hit.
[Graphical view]
SUPFAMiSSF57492. SSF57492. 1 hit.
PROSITEiPS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus."
    Hauser F., Poulsom R., Chinery R., Rogers L.A., Hanby A.M., Wright N.A., Hoffmann W.
    Proc. Natl. Acad. Sci. U.S.A. 90:6961-6965(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Small intestine.
  2. "The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3."
    Seib T., Blin N., Hilgert K., Seifert M., Theisinger B., Engel M., Dooley S., Zang K.D., Welter C.
    Genomics 40:200-202(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Refined localization of autosomal recessive nonsyndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure, and exclusion of six known genes in the region."
    Berry A., Scott H.S., Kudoh J., Talior I., Korostishevsky M., Wattenhofer M., Guipponi M., Barras C., Rossier C., Shibuya K., Wang J., Kawasaki K., Asakawa S., Minoshima S., Shimizu N., Antonarakis S.E., Bonne-Tamir B.
    Genomics 68:22-29(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The DNA sequence of human chromosome 21."
    Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
    , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
    Nature 405:311-319(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  6. "mRNA 5' region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs."
    Casadei R., Strippoli P., D'Addabbo P., Canaider S., Lenzi L., Vitale L., Giannone S., Frabetti F., Facchin F., Carinci P., Zannotti M.
    Gene 321:185-193(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-51.
    Tissue: Heart.
  7. "Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion."
    Podolsky D.K., Lynch-Devaney K., Stow J.L., Oates P., Murgue B., Debeaumont M., Sands B.E., Mahida Y.R.
    J. Biol. Chem. 268:6694-6702(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-80, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Colon.
  8. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-36.
  9. Cited for: PROTEIN SEQUENCE OF 22-37, IDENTIFICATION BY MASS SPECTROMETRY.
  10. "Coexistence of intestinal trefoil factor (hITF) and oxytocin in magnocellular neurons in the human hypothalamus."
    Griepentrog T., Bauer M., Hornstein C., Sauer H., Jirikowski G.F.
    Horm. Metab. Res. 32:121-124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  11. "Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor."
    Oertel M., Graness A., Thim L., Buhling F., Kalbacher H., Hoffmann W.
    Am. J. Respir. Cell Mol. Biol. 25:418-424(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Incorporation of mannose and glucose into prenylphosphate sugars in isolated human platelet membranes."
    De Luca S.
    Biochim. Biophys. Acta 498:341-348(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 22-80.
  13. "Solution structure of the disulfide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins."
    Muskett F.W., May F.E., Westley B.R., Feeney J.
    Biochemistry 42:15139-15147(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 22-80, SUBUNIT.

Entry informationi

Entry nameiTFF3_HUMAN
AccessioniPrimary (citable) accession number: Q07654
Secondary accession number(s): E9PBB5, Q96NX0, Q9UDA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1994
Last sequence update: September 30, 1994
Last modified: March 3, 2015
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.