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Q07652

- CAC1E_RAT

UniProt

Q07652 - CAC1E_RAT

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Protein
Voltage-dependent R-type calcium channel subunit alpha-1E
Gene
Cacna1e, Cach6, Cacnl1a6
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel, and partially by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), omega-conotoxin-GVIA (omega-CTx-GVIA), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei260 – 2601Calcium ion selectivity and permeability By similarity
Sitei608 – 6081Calcium ion selectivity and permeability By similarity
Sitei1324 – 13241Calcium ion selectivity and permeability By similarity
Sitei1615 – 16151Calcium ion selectivity and permeability By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi377 – 38812 By similarity
Add
BLAST
Calcium bindingi1704 – 171512 By similarity
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. high voltage-gated calcium channel activity Source: RefGenome
  3. low voltage-gated calcium channel activity Source: RGD
  4. voltage-gated calcium channel activity Source: RGD

GO - Biological processi

  1. calcium ion import Source: RGD
  2. membrane depolarization during action potential Source: RefGenome
  3. synaptic transmission Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent R-type calcium channel subunit alpha-1E
Alternative name(s):
BII
Brain calcium channel II
Calcium channel, L type, alpha-1 polypeptide, isoform 6
RBE-II
RBE2
Voltage-gated calcium channel subunit alpha Cav2.3
Gene namesi
Name:Cacna1e
Synonyms:Cach6, Cacnl1a6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2246. Cacna1e.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4040Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei41 – 5919Helical; Name=S1 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini60 – 7819Extracellular Reviewed prediction
Add
BLAST
Transmembranei79 – 9719Helical; Name=S2 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini98 – 10912Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei110 – 12415Helical; Name=S3 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini125 – 13612Extracellular Reviewed prediction
Add
BLAST
Transmembranei137 – 15620Helical; Name=S4 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini157 – 17418Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei175 – 19521Helical; Name=S5 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini196 – 27782Extracellular Reviewed prediction
Add
BLAST
Transmembranei278 – 30124Helical; Name=S6 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini302 – 427126Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei428 – 44720Helical; Name=S1 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini448 – 46013Extracellular Reviewed prediction
Add
BLAST
Transmembranei461 – 48020Helical; Name=S2 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini481 – 4899Cytoplasmic Reviewed prediction
Transmembranei490 – 50819Helical; Name=S3 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini509 – 51810Extracellular Reviewed prediction
Transmembranei519 – 53719Helical; Name=S4 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini538 – 55619Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei557 – 57620Helical; Name=S5 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini577 – 62953Extracellular Reviewed prediction
Add
BLAST
Transmembranei630 – 65425Helical; Name=S6 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini655 – 1100446Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1101 – 111717Helical; Name=S1 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1118 – 114124Extracellular Reviewed prediction
Add
BLAST
Transmembranei1142 – 116120Helical; Name=S2 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1162 – 11698Cytoplasmic Reviewed prediction
Transmembranei1170 – 119223Helical; Name=S3 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1193 – 120614Extracellular Reviewed prediction
Add
BLAST
Transmembranei1207 – 122418Helical; Name=S4 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1225 – 124319Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1244 – 126320Helical; Name=S5 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1264 – 135087Extracellular Reviewed prediction
Add
BLAST
Transmembranei1351 – 137424Helical; Name=S6 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1375 – 143157Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1432 – 145019Helical; Name=S1 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1451 – 146717Extracellular Reviewed prediction
Add
BLAST
Transmembranei1468 – 148518Helical; Name=S2 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1486 – 14938Cytoplasmic Reviewed prediction
Transmembranei1494 – 151219Helical; Name=S3 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1513 – 152311Extracellular Reviewed prediction
Add
BLAST
Transmembranei1524 – 154219Helical; Name=S4 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1543 – 156119Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1562 – 158120Helical; Name=S5 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1582 – 165069Extracellular Reviewed prediction
Add
BLAST
Transmembranei1651 – 167626Helical; Name=S6 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1677 – 2222546Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. perikaryon Source: RGD
  2. voltage-gated calcium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22222222Voltage-dependent R-type calcium channel subunit alpha-1E
PRO_0000053941Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi205 – 2051N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1518 – 15181N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1523 – 15231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1641 – 16411N-linked (GlcNAc...) Reviewed prediction
Modified residuei1686 – 16861Phosphoserine; by PKA Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ07652.
PRIDEiQ07652.

PTM databases

PhosphoSiteiQ07652.

Expressioni

Tissue specificityi

Expressed in central nervous system and in insulinoma.

Gene expression databases

GenevestigatoriQ07652.

Interactioni

Subunit structurei

Interacts with EFHC1. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity.

Protein-protein interaction databases

DIPiDIP-46236N.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1818 – 183518

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DVKX-ray2.30B1818-1837[»]
ProteinModelPortaliQ07652.

Miscellaneous databases

EvolutionaryTraceiQ07652.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati27 – 305279I
Add
BLAST
Repeati413 – 657245II
Add
BLAST
Repeati1092 – 1378287III
Add
BLAST
Repeati1415 – 1678264IV
Add
BLAST
Domaini1691 – 172636EF-hand
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 34218Binding to the beta subunit By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi667 – 6726Poly-Glu
Compositional biasi699 – 7046Poly-Arg
Compositional biasi718 – 7236Poly-Arg
Compositional biasi1058 – 10647Poly-Glu
Compositional biasi1180 – 11834Poly-Val
Compositional biasi2193 – 21964Poly-Arg

Domaini

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Contains 1 EF-hand domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOGENOMiHOG000231530.
HOVERGENiHBG050763.
PhylomeDBiQ07652.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR002048. EF_hand_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005449. VDCC_R_a1su.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF57. PTHR10037:SF57. 1 hit.
PfamiPF08763. Ca_chan_IQ. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01633. RVDCCALPHA1.
SMARTiSM01062. Ca_chan_IQ. 1 hit.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07652-1 [UniParc]FASTAAdd to Basket

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MALYNPIPVR QNCFTVNRSL FIFGEDNIVR KYAKKLIDWP PFEYMILATI     50
IANCIVLALE QHLPEDDKTP MSRRLEKTEP YFIGIFCFEA GIKIVALGFI 100
FHKGSYLRNG WNVMDFIVVL SGILATAGTH FNTHVDLRTL RAVRVLRPLK 150
LVSGIPSLQI VLKSIMKAMV PLLQIGLLLF FAILMFAIIG LEFYSGKLHR 200
ACFMNNSGIL EGFDPPHPCG VQGCPAGYEC KDWIGPNDGI TQFDNILFAV 250
LTVFQCITME GWTTVLYNTN DALGATWNWL YFIPLIIIGS FFVLNLVLGV 300
LSGEFAKERE RVENRRAFMK LRRQQQIERE LNGYRAWIDK AEEVMLAEEN 350
KNSGTSALEV LRRATIKRSR TEAMTRDSSD EHCVDISSVG TPLARASIKS 400
TKVDGASYFR HKERLLRISI RHMVKSQVFY WIVLSVVALN TACVAIVHHN 450
QPQWLTHLLY YAEFLFLGLF LLEMSLKMYG MGPRLYFHSS FNCFDFGVTV 500
GSIFEVVWAI FRPGTSFGIS VLRALRLLRI FKITKYWASL RNLVVSLMSS 550
MKSIISLLFL LFLFIVVFAL LGMQLFGGRF NFNDGTPSAN FDTFPAAIMT 600
VFQILTGEDW NEVMYNGIRS QGGVSSGMWS AIYFIVLTLF GNYTLLNVFL 650
AIAVDNLANA QELTKDEQEE EEAFNQKHAL QKAKEVSPMS APNMPSIERD 700
RRRRHHMSMW EPRSSHLRER RRRHHMSVWE QRTSQLRRHM QMSSQEALNK 750
EEAPPMNPLN PLNPLSPLNP LNAHPSLYRR PRPIEGLALG LGLEKCEEER 800
ISRGGSLKGD IGGLTSVLDN QRSPLSLGKR EPPWLPRSCH GNCDPTQQET 850
GGGETVVTFE DRARHRQSQR RSRHRRVRTE GKESASASRS RSASQERSLD 900
EGVSIDGEKE HEPQSSHRSK EPTIHEEERT QDLRRTNSLM VPRGSGLVGA 950
LDEAETPLVQ PQPELEVGKD AALTEQEAEG SSEQALLADV QLDVGRGISQ 1000
SEPDLSCMTT NMDKATTEST SVTVAIPDVD PLVDSTVVNI SNKTDGEASP 1050
LKEAETKEEE EEVEKKKQKK EKRETGKAMV PHSSMFIFST TNPIRKACHY 1100
IVNLRYFEMC ILLVIAASSI ALAAEDPVLT NSERNKVLRY FDYVFTGVFT 1150
FEMVIKMIDQ GLILQDGSYF RDLWNILDFV VVVGALVAFA LANALGTNKG 1200
RDIKTIKSLR VLRVLRPLKT IKRLPKLKAV FDCVVTSLKN VFNILIVYKL 1250
FMFIFAVIAV QLFKGKFFYC TDSSKDTEKE CIGNYVDHEK NKMEVKGREW 1300
KRHEFHYDNI IWALLTLFTV STGEGWPQVL QHSVDVTEED RGPSRSNRME 1350
MSIFYVVYFV VFPFFFVNIF VALIIITFQE QGDKMMEECS LEKNERACID 1400
FAISAKPLTR YMPQNRHTFQ YRVWHFVVSP SFEYTIMAMI ALNTVVLMMK 1450
YYSAPWTYEL ALKYLNIAFT MVFSLECVLK VIAFGFLNYF RDTWNIFDFI 1500
TVIGSITEII LTDSKLVNTS GFNMSFLKLF RAARLIKLLR QGYTIRILLW 1550
TFVQSFKALP YVCLLIAMLF FIYAIIGMQV FGNIKLDEES HINRHNNFRS 1600
FFGSLMLLFR SATGEAWQEI MLSCLGEKGC EPDTTAPSGQ NESERCGTDL 1650
AYVYFVSFIF FCSFLMLNLF VAVIMDNFEY LTRDSSILGP HHLDEFVRVW 1700
AEYDRAACGR IHYTEMYEML TLMSPPLGLG KRCPSKVAYK RLVLMNMPVA 1750
EDMTVHFTST LMALIRTALD IKIAKGGADR QQLDSELQKE TLAIWPHLSQ 1800
KMLDLLVPMP KASDLTVGKI YAAMMIMDYY KQSKVKKQRQ QLEEQKNAPM 1850
FQRMEPSSLP QEIISNAKAL PYLQQDPVSG LSGRSGYPSM SPLSPQEIFQ 1900
LACMDPADDG QFQEQQSLVV TDPSSMRRSF STIRDKRSNS SWLEEFSMER 1950
SSENTYKSRR RSYHSSLRLS AHRLNSDSGH KSDTHRSGGR ERGRSKERKH 2000
LLSPDVSRCN SEERGTQADW ESPERRQSRS PSEGRSQTPN RQGTGSLSES 2050
SIPSISDTST PRRSRRQLPP VPPKPRPLLS YSSLMRHTGG ISPPPDGSEG 2100
GSPLASQALE SNSACLTESS NSLHPQQGQH PSPQHYISEP YLALHEDSHA 2150
SDCGEEETLT FEAAVATSLG RSNTIGSAPP LRHSWQMPNG HYRRRRLGGL 2200
GLAMMCGAVS DLLSDTEEDD KC 2222
Length:2,222
Mass (Da):252,116
Last modified:October 1, 1996 - v1
Checksum:iDF6452A2175CEB19
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L15453 mRNA. Translation: AAA40855.1.
PIRiA37490.
UniGeneiRn.10742.

Genome annotation databases

UCSCiRGD:2246. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L15453 mRNA. Translation: AAA40855.1 .
PIRi A37490.
UniGenei Rn.10742.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3DVK X-ray 2.30 B 1818-1837 [» ]
ProteinModelPortali Q07652.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-46236N.

Chemistry

BindingDBi Q07652.
GuidetoPHARMACOLOGYi 534.

PTM databases

PhosphoSitei Q07652.

Proteomic databases

PaxDbi Q07652.
PRIDEi Q07652.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2246. rat.

Organism-specific databases

RGDi 2246. Cacna1e.

Phylogenomic databases

eggNOGi COG1226.
HOGENOMi HOG000231530.
HOVERGENi HBG050763.
PhylomeDBi Q07652.

Miscellaneous databases

EvolutionaryTracei Q07652.
PROi Q07652.

Gene expression databases

Genevestigatori Q07652.

Family and domain databases

Gene3Di 1.20.120.350. 4 hits.
InterProi IPR027359. Channel_four-helix_dom.
IPR002048. EF_hand_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005449. VDCC_R_a1su.
IPR002077. VDCCAlpha1.
[Graphical view ]
PANTHERi PTHR10037:SF57. PTHR10037:SF57. 1 hit.
Pfami PF08763. Ca_chan_IQ. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view ]
PRINTSi PR00167. CACHANNEL.
PR01633. RVDCCALPHA1.
SMARTi SM01062. Ca_chan_IQ. 1 hit.
[Graphical view ]
PROSITEi PS50222. EF_HAND_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure and functional expression of a member of the low voltage-activated calcium channel family."
    Soong T.W., Stea A., Hodson C.D., Dubel S.J., Vincent S.R., Snutch T.P.
    Science 260:1133-1136(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiCAC1E_RAT
AccessioniPrimary (citable) accession number: Q07652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi