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Q07646

- MEST_MOUSE

UniProt

Q07646 - MEST_MOUSE

Protein

Mesoderm-specific transcript protein

Gene

Mest

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of lipid storage Source: UniProtKB
    2. response to retinoic acid Source: BHF-UCL

    Keywords - Molecular functioni

    Hydrolase

    Protein family/group databases

    MEROPSiS33.972.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mesoderm-specific transcript protein (EC:3.-.-.-)
    Alternative name(s):
    Paternally-expressed gene 1 protein
    Gene namesi
    Name:Mest
    Synonyms:Peg1
    ORF Names:121a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:96968. Mest.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Mesoderm-specific transcript proteinPRO_0000284419Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ07646.
    PRIDEiQ07646.

    Expressioni

    Tissue specificityi

    Expressed in mesodermal tissues. Isoform 1 is exclusively expressed from the paternal allele in all fetal tissues and cell lines examined, whereas isoform 2 is preferentially expressed from the paternal allele in a tissue-type-specific manner.1 Publication

    Developmental stagei

    Expressed in the nascent mesoderm of gastrulating embryos. At 7.0 dpc expression detected in both the intraembryonic mesoderm and the extraembryonic mesoderm cells of the amniotic fold. At 8.5 dpc expressed predominantly in the head mesenchyme, allantois, and the mesodermal layer of the amnion, chorion and yolk. In 9.5 dpc embryos highly expressed in the mesenchymal tissues, presomitic paraxial mesoderm, heart and branchial arches.1 Publication

    Gene expression databases

    ArrayExpressiQ07646.
    BgeeiQ07646.
    CleanExiMM_MEST.
    GenevestigatoriQ07646.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07646.
    SMRiQ07646. Positions 48-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei63 – 8321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei266 – 28621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi98 – 1036RVIALD

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0596.
    GeneTreeiENSGT00510000047602.
    HOGENOMiHOG000049677.
    HOVERGENiHBG052816.
    InParanoidiQ07646.
    OMAiSVSIPIH.
    PhylomeDBiQ07646.
    TreeFamiTF329307.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000639. Epox_hydrolase-like.
    [Graphical view]
    PRINTSiPR00412. EPOXHYDRLASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q07646-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKTSGKFF    50
    TYKGLRIFYQ DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI 100
    ALDFLGFGFS DKPRPHQYSI FEQASIVESL LRHLGLQNRR INLLSHDYGD 150
    IVAQELLYRY KQNRSGRLTI KSLCLSNGGI FPETHRPLLL QKLLKDGGVL 200
    SPILTRLMNF FVFSRGLTPV FGPYTRPTES ELWDMWAVIR NNDGNLVIDS 250
    LLQYINQRKK FRRRWVGALA SVSIPIHFIY GPLDPINPYP EFLELYRKTL 300
    PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF 335
    Length:335
    Mass (Da):38,907
    Last modified:November 1, 1996 - v1
    Checksum:iF5F394F46152E712
    GO
    Isoform 2 (identifier: Q07646-2) [UniParc]FASTAAdd to Basket

    Also known as: MEST intronic transcript 1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-9: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:326
    Mass (Da):37,668
    Checksum:i1C4DD363B5D3421A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti43 – 431W → C in BAC28891. (PubMed:16141072)Curated
    Sequence conflicti171 – 1711K → N in AAM78507. (PubMed:12095916)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 99Missing in isoform 2. 1 PublicationVSP_024534

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16262 mRNA. Translation: BAA03795.1.
    AF482999 mRNA. Translation: AAM78507.1.
    AK032881 mRNA. Translation: BAC28068.1.
    AK034949 mRNA. Translation: BAC28891.1.
    AK168714 mRNA. Translation: BAE40556.1.
    AK168732 mRNA. Translation: BAE40573.1.
    AK168743 mRNA. Translation: BAE40584.1.
    AK168995 mRNA. Translation: BAE40794.1.
    AK169095 mRNA. Translation: BAE40878.1.
    AK169266 mRNA. Translation: BAE41027.1.
    BC004019 mRNA. Translation: AAH04019.1.
    BC006639 mRNA. Translation: AAH06639.1.
    AF017994 Genomic DNA. Translation: AAC53396.1.
    CCDSiCCDS19979.1. [Q07646-1]
    RefSeqiNP_001239221.1. NM_001252292.1.
    NP_001239222.1. NM_001252293.1. [Q07646-2]
    NP_032616.1. NM_008590.2. [Q07646-1]
    UniGeneiMm.335639.

    Genome annotation databases

    EnsembliENSMUST00000163949; ENSMUSP00000129639; ENSMUSG00000051855. [Q07646-1]
    GeneIDi17294.
    KEGGimmu:17294.
    UCSCiuc009bfs.2. mouse. [Q07646-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16262 mRNA. Translation: BAA03795.1 .
    AF482999 mRNA. Translation: AAM78507.1 .
    AK032881 mRNA. Translation: BAC28068.1 .
    AK034949 mRNA. Translation: BAC28891.1 .
    AK168714 mRNA. Translation: BAE40556.1 .
    AK168732 mRNA. Translation: BAE40573.1 .
    AK168743 mRNA. Translation: BAE40584.1 .
    AK168995 mRNA. Translation: BAE40794.1 .
    AK169095 mRNA. Translation: BAE40878.1 .
    AK169266 mRNA. Translation: BAE41027.1 .
    BC004019 mRNA. Translation: AAH04019.1 .
    BC006639 mRNA. Translation: AAH06639.1 .
    AF017994 Genomic DNA. Translation: AAC53396.1 .
    CCDSi CCDS19979.1. [Q07646-1 ]
    RefSeqi NP_001239221.1. NM_001252292.1.
    NP_001239222.1. NM_001252293.1. [Q07646-2 ]
    NP_032616.1. NM_008590.2. [Q07646-1 ]
    UniGenei Mm.335639.

    3D structure databases

    ProteinModelPortali Q07646.
    SMRi Q07646. Positions 48-333.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S33.972.

    Proteomic databases

    PaxDbi Q07646.
    PRIDEi Q07646.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000163949 ; ENSMUSP00000129639 ; ENSMUSG00000051855 . [Q07646-1 ]
    GeneIDi 17294.
    KEGGi mmu:17294.
    UCSCi uc009bfs.2. mouse. [Q07646-1 ]

    Organism-specific databases

    CTDi 4232.
    MGIi MGI:96968. Mest.

    Phylogenomic databases

    eggNOGi COG0596.
    GeneTreei ENSGT00510000047602.
    HOGENOMi HOG000049677.
    HOVERGENi HBG052816.
    InParanoidi Q07646.
    OMAi SVSIPIH.
    PhylomeDBi Q07646.
    TreeFami TF329307.

    Miscellaneous databases

    ChiTaRSi MEST. mouse.
    NextBioi 291822.
    PROi Q07646.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q07646.
    Bgeei Q07646.
    CleanExi MM_MEST.
    Genevestigatori Q07646.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000639. Epox_hydrolase-like.
    [Graphical view ]
    PRINTSi PR00412. EPOXHYDRLASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel mesoderm-specific cDNA isolated from a mouse embryonal carcinoma cell line."
      Sado T., Nakajima N., Tada M., Takagi N.
      Dev. Growth Differ. 35:551-560(1993)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
    2. "Identification and characterization of an imprinted antisense RNA (MESTIT1) in the human MEST locus on chromosome 7q32."
      Nakabayashi K., Bentley L., Hitchins M.P., Mitsuya K., Meguro M., Minagawa S., Bamforth J.S., Stanier P., Preece M., Weksberg R., Oshimura M., Moore G.E., Scherer S.W.
      Hum. Mol. Genet. 11:1743-1756(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
      Strain: C57BL/6J.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Amnion, Embryo, Heart, Kidney and Wolffian duct.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Czech II.
      Tissue: Mammary tumor.
    5. "Genomic structure and parent-of-origin-specific methylation of Peg1."
      Lefebvre L., Viville S., Barton S.C., Ishino F., Surani M.A.
      Hum. Mol. Genet. 6:1907-1915(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-60.
      Strain: 129/Sv.
    6. "Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization."
      Kaneko-Ishino T., Kuroiwa Y., Miyoshi N., Kohda T., Suzuki R., Yokoyama M., Viville S., Barton S.C., Ishino F., Surani M.A.
      Nat. Genet. 11:52-59(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
      Strain: 129/Sv.
    7. "Mesoderm-specific transcript is associated with fat mass expansion in response to a positive energy balance."
      Nikonova L., Koza R.A., Mendoza T., Chao P.-M., Curley J.P., Kozak L.P.
      FASEB J. 22:3925-3937(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiMEST_MOUSE
    AccessioniPrimary (citable) accession number: Q07646
    Secondary accession number(s): Q792T8
    , Q8BS88, Q8K463, Q99KT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3