Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mesoderm-specific transcript protein

Gene

Mest

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  1. regulation of lipid storage Source: UniProtKB
  2. response to retinoic acid Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Protein family/group databases

MEROPSiS33.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Mesoderm-specific transcript protein (EC:3.-.-.-)
Alternative name(s):
Paternally-expressed gene 1 protein
Gene namesi
Name:Mest
Synonyms:Peg1
ORF Names:121a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:96968. Mest.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Transmembranei63 – 8321HelicalSequence AnalysisAdd
BLAST
Transmembranei266 – 28621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. extracellular vesicular exosome Source: MGI
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Mesoderm-specific transcript proteinPRO_0000284419Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ07646.
PaxDbiQ07646.
PRIDEiQ07646.

Expressioni

Tissue specificityi

Expressed in mesodermal tissues. Isoform 1 is exclusively expressed from the paternal allele in all fetal tissues and cell lines examined, whereas isoform 2 is preferentially expressed from the paternal allele in a tissue-type-specific manner.1 Publication

Developmental stagei

Expressed in the nascent mesoderm of gastrulating embryos. At 7.0 dpc expression detected in both the intraembryonic mesoderm and the extraembryonic mesoderm cells of the amniotic fold. At 8.5 dpc expressed predominantly in the head mesenchyme, allantois, and the mesodermal layer of the amnion, chorion and yolk. In 9.5 dpc embryos highly expressed in the mesenchymal tissues, presomitic paraxial mesoderm, heart and branchial arches.1 Publication

Gene expression databases

BgeeiQ07646.
CleanExiMM_MEST.
ExpressionAtlasiQ07646. baseline and differential.
GenevestigatoriQ07646.

Structurei

3D structure databases

ProteinModelPortaliQ07646.
SMRiQ07646. Positions 48-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi98 – 1036RVIALD

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0596.
GeneTreeiENSGT00510000047602.
HOGENOMiHOG000049677.
HOVERGENiHBG052816.
InParanoidiQ07646.
OMAiCDMLGFG.
PhylomeDBiQ07646.
TreeFamiTF329307.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q07646-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKTSGKFF
60 70 80 90 100
TYKGLRIFYQ DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI
110 120 130 140 150
ALDFLGFGFS DKPRPHQYSI FEQASIVESL LRHLGLQNRR INLLSHDYGD
160 170 180 190 200
IVAQELLYRY KQNRSGRLTI KSLCLSNGGI FPETHRPLLL QKLLKDGGVL
210 220 230 240 250
SPILTRLMNF FVFSRGLTPV FGPYTRPTES ELWDMWAVIR NNDGNLVIDS
260 270 280 290 300
LLQYINQRKK FRRRWVGALA SVSIPIHFIY GPLDPINPYP EFLELYRKTL
310 320 330
PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF
Length:335
Mass (Da):38,907
Last modified:November 1, 1996 - v1
Checksum:iF5F394F46152E712
GO
Isoform 2 (identifier: Q07646-2) [UniParc]FASTAAdd to basket

Also known as: MEST intronic transcript 1

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Note: No experimental confirmation available.

Show »
Length:326
Mass (Da):37,668
Checksum:i1C4DD363B5D3421A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431W → C in BAC28891 (PubMed:16141072).Curated
Sequence conflicti171 – 1711K → N in AAM78507 (PubMed:12095916).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 99Missing in isoform 2. 1 PublicationVSP_024534

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16262 mRNA. Translation: BAA03795.1.
AF482999 mRNA. Translation: AAM78507.1.
AK032881 mRNA. Translation: BAC28068.1.
AK034949 mRNA. Translation: BAC28891.1.
AK168714 mRNA. Translation: BAE40556.1.
AK168732 mRNA. Translation: BAE40573.1.
AK168743 mRNA. Translation: BAE40584.1.
AK168995 mRNA. Translation: BAE40794.1.
AK169095 mRNA. Translation: BAE40878.1.
AK169266 mRNA. Translation: BAE41027.1.
BC004019 mRNA. Translation: AAH04019.1.
BC006639 mRNA. Translation: AAH06639.1.
AF017994 Genomic DNA. Translation: AAC53396.1.
CCDSiCCDS19979.1. [Q07646-1]
RefSeqiNP_001239221.1. NM_001252292.1.
NP_001239222.1. NM_001252293.1. [Q07646-2]
NP_032616.1. NM_008590.2. [Q07646-1]
UniGeneiMm.335639.

Genome annotation databases

EnsembliENSMUST00000163949; ENSMUSP00000129639; ENSMUSG00000051855. [Q07646-1]
GeneIDi17294.
KEGGimmu:17294.
UCSCiuc009bfs.2. mouse. [Q07646-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16262 mRNA. Translation: BAA03795.1.
AF482999 mRNA. Translation: AAM78507.1.
AK032881 mRNA. Translation: BAC28068.1.
AK034949 mRNA. Translation: BAC28891.1.
AK168714 mRNA. Translation: BAE40556.1.
AK168732 mRNA. Translation: BAE40573.1.
AK168743 mRNA. Translation: BAE40584.1.
AK168995 mRNA. Translation: BAE40794.1.
AK169095 mRNA. Translation: BAE40878.1.
AK169266 mRNA. Translation: BAE41027.1.
BC004019 mRNA. Translation: AAH04019.1.
BC006639 mRNA. Translation: AAH06639.1.
AF017994 Genomic DNA. Translation: AAC53396.1.
CCDSiCCDS19979.1. [Q07646-1]
RefSeqiNP_001239221.1. NM_001252292.1.
NP_001239222.1. NM_001252293.1. [Q07646-2]
NP_032616.1. NM_008590.2. [Q07646-1]
UniGeneiMm.335639.

3D structure databases

ProteinModelPortaliQ07646.
SMRiQ07646. Positions 48-333.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS33.972.

Proteomic databases

MaxQBiQ07646.
PaxDbiQ07646.
PRIDEiQ07646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000163949; ENSMUSP00000129639; ENSMUSG00000051855. [Q07646-1]
GeneIDi17294.
KEGGimmu:17294.
UCSCiuc009bfs.2. mouse. [Q07646-1]

Organism-specific databases

CTDi4232.
MGIiMGI:96968. Mest.

Phylogenomic databases

eggNOGiCOG0596.
GeneTreeiENSGT00510000047602.
HOGENOMiHOG000049677.
HOVERGENiHBG052816.
InParanoidiQ07646.
OMAiCDMLGFG.
PhylomeDBiQ07646.
TreeFamiTF329307.

Miscellaneous databases

ChiTaRSiMest. mouse.
NextBioi291822.
PROiQ07646.
SOURCEiSearch...

Gene expression databases

BgeeiQ07646.
CleanExiMM_MEST.
ExpressionAtlasiQ07646. baseline and differential.
GenevestigatoriQ07646.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel mesoderm-specific cDNA isolated from a mouse embryonal carcinoma cell line."
    Sado T., Nakajima N., Tada M., Takagi N.
    Dev. Growth Differ. 35:551-560(1992)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
  2. "Identification and characterization of an imprinted antisense RNA (MESTIT1) in the human MEST locus on chromosome 7q32."
    Nakabayashi K., Bentley L., Hitchins M.P., Mitsuya K., Meguro M., Minagawa S., Bamforth J.S., Stanier P., Preece M., Weksberg R., Oshimura M., Moore G.E., Scherer S.W.
    Hum. Mol. Genet. 11:1743-1756(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
    Strain: C57BL/6J.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Amnion, Embryo, Heart, Kidney and Wolffian duct.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.
    Tissue: Mammary tumor.
  5. "Genomic structure and parent-of-origin-specific methylation of Peg1."
    Lefebvre L., Viville S., Barton S.C., Ishino F., Surani M.A.
    Hum. Mol. Genet. 6:1907-1915(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-60.
    Strain: 129/Sv.
  6. "Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization."
    Kaneko-Ishino T., Kuroiwa Y., Miyoshi N., Kohda T., Suzuki R., Yokoyama M., Viville S., Barton S.C., Ishino F., Surani M.A.
    Nat. Genet. 11:52-59(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
    Strain: 129/Sv.
  7. "Mesoderm-specific transcript is associated with fat mass expansion in response to a positive energy balance."
    Nikonova L., Koza R.A., Mendoza T., Chao P.-M., Curley J.P., Kozak L.P.
    FASEB J. 22:3925-3937(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMEST_MOUSE
AccessioniPrimary (citable) accession number: Q07646
Secondary accession number(s): Q792T8
, Q8BS88, Q8K463, Q99KT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.