Q07646 (MEST_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mesoderm-specific transcript protein
EC=3.-.-.-

Alternative name(s):
Paternally-expressed gene 1 protein

Gene names

Name:	Mest
Synonyms:	Peg1
ORF Names:	121a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.7.
Tissue specificity	Expressed in mesodermal tissues. Isoform 1 is exclusively expressed from the paternal allele in all fetal tissues and cell lines examined, whereas isoform 2 is preferentially expressed from the paternal allele in a tissue-type-specific manner. Ref.6
Developmental stage	Expressed in the nascent mesoderm of gastrulating embryos. At 7.0 dpc expression detected in both the intraembryonic mesoderm and the extraembryonic mesoderm cells of the amniotic fold. At 8.5 dpc expressed predominantly in the head mesenchyme, allantois, and the mesodermal layer of the amnion, chorion and yolk. In 9.5 dpc embryos highly expressed in the mesenchymal tissues, presomitic paraxial mesoderm, heart and branchial arches. Ref.1
Sequence similarities	Belongs to the AB hydrolase superfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC regulation of lipid storage Inferred from mutant phenotype Ref.7. Source: UniProtKB response to retinoic acid Inferred from direct assay PubMed 18439490. Source: BHF-UCL
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.7. Source: UniProtKB endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q07646-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q07646-2)

Also known as: MEST intronic transcript 1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Mesoderm-specific transcript protein		PRO_0000284419
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Transmembrane	63 – 83	21	Helical; Potential
	Transmembrane	266 – 286	21	Helical; Potential
	Motif	98 – 103	6	RVIALD
Amino acid modifications
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 9	9	Missing in isoform 2.		VSP_024534
Experimental info
	Sequence conflict	43	1	W → C in BAC28891. Ref.3
	Sequence conflict	171	1	K → N in AAM78507. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F5F394F46152E712	FASTA	335	38,907
	10 20 30 40 50 60 MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKTSGKFF TYKGLRIFYQ 70 80 90 100 110 120 DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI ALDFLGFGFS DKPRPHQYSI 130 140 150 160 170 180 FEQASIVESL LRHLGLQNRR INLLSHDYGD IVAQELLYRY KQNRSGRLTI KSLCLSNGGI 190 200 210 220 230 240 FPETHRPLLL QKLLKDGGVL SPILTRLMNF FVFSRGLTPV FGPYTRPTES ELWDMWAVIR 250 260 270 280 290 300 NNDGNLVIDS LLQYINQRKK FRRRWVGALA SVSIPIHFIY GPLDPINPYP EFLELYRKTL 310 320 330 PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF
	Isoform 2 (MEST intronic transcript 1) [UniParc]. Checksum: 1C4DD363B5D3421A	FASTA	326	37,668
	10 20 30 40 50 60 MREWWVQVGL LAVPLLAAYL HIPPPQLSPA LHSWKTSGKF FTYKGLRIFY QDSVGVVGSP 70 80 90 100 110 120 EIVVLLHGFP TSSYDWYKIW EGLTLRFHRV IALDFLGFGF SDKPRPHQYS IFEQASIVES 130 140 150 160 170 180 LLRHLGLQNR RINLLSHDYG DIVAQELLYR YKQNRSGRLT IKSLCLSNGG IFPETHRPLL 190 200 210 220 230 240 LQKLLKDGGV LSPILTRLMN FFVFSRGLTP VFGPYTRPTE SELWDMWAVI RNNDGNLVID 250 260 270 280 290 300 SLLQYINQRK KFRRRWVGAL ASVSIPIHFI YGPLDPINPY PEFLELYRKT LPRSTVSILD 310 320 DHISHYPQLE DPMGFLNAYM GFINSF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel mesoderm-specific cDNA isolated from a mouse embryonal carcinoma cell line." Sado T., Nakajima N., Tada M., Takagi N. Dev. Growth Differ. 35:551-560(1993) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
[2]	"Identification and characterization of an imprinted antisense RNA (MESTIT1) in the human MEST locus on chromosome 7q32." Nakabayashi K., Bentley L., Hitchins M.P., Mitsuya K., Meguro M., Minagawa S., Bamforth J.S., Stanier P., Preece M., Weksberg R., Oshimura M., Moore G.E., Scherer S.W. Hum. Mol. Genet. 11:1743-1756(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING. Strain: C57BL/6J.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Amnion, Embryo, Heart, Kidney and Wolffian duct.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Czech II. Tissue: Mammary tumor.
[5]	"Genomic structure and parent-of-origin-specific methylation of Peg1." Lefebvre L., Viville S., Barton S.C., Ishino F., Surani M.A. Hum. Mol. Genet. 6:1907-1915(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-60. Strain: 129/Sv.
[6]	"Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization." Kaneko-Ishino T., Kuroiwa Y., Miyoshi N., Kohda T., Suzuki R., Yokoyama M., Viville S., Barton S.C., Ishino F., Surani M.A. Nat. Genet. 11:52-59(1995) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY. Strain: 129/Sv.
[7]	"Mesoderm-specific transcript is associated with fat mass expansion in response to a positive energy balance." Nikonova L., Koza R.A., Mendoza T., Chao P.-M., Curley J.P., Kozak L.P. FASEB J. 22:3925-3937(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16262 mRNA. Translation: BAA03795.1. AF482999 mRNA. Translation: AAM78507.1. AK032881 mRNA. Translation: BAC28068.1. AK034949 mRNA. Translation: BAC28891.1. AK168714 mRNA. Translation: BAE40556.1. AK168732 mRNA. Translation: BAE40573.1. AK168743 mRNA. Translation: BAE40584.1. AK168995 mRNA. Translation: BAE40794.1. AK169095 mRNA. Translation: BAE40878.1. AK169266 mRNA. Translation: BAE41027.1. BC004019 mRNA. Translation: AAH04019.1. BC006639 mRNA. Translation: AAH06639.1. AF017994 Genomic DNA. Translation: AAC53396.1.
IPI	IPI00118545. IPI00754963.
RefSeq	NP_001239221.1. NM_001252292.1. NP_001239222.1. NM_001252293.1. NP_032616.1. NM_008590.2.
UniGene	Mm.335639.
3D structure databases
ProteinModelPortal	Q07646.
SMR	Q07646. Positions 48-189.
ModBase	Search...
Protein family/group databases
MEROPS	S33.972.
Proteomic databases
PaxDb	Q07646.
PRIDE	Q07646.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000163949; ENSMUSP00000129639; ENSMUSG00000051855.
GeneID	17294.
KEGG	mmu:17294.
UCSC	uc009bfs.1. mouse. uc009bft.1. mouse.
Organism-specific databases
CTD	4232.
MGI	MGI:96968. Mest.
Phylogenomic databases
eggNOG	COG0596.
GeneTree	ENSGT00510000047602.
HOGENOM	HOG000049677.
HOVERGEN	HBG052816.
InParanoid	Q07646.
OMA	YRFKQNR.
Gene expression databases
ArrayExpress	Q07646.
Bgee	Q07646.
CleanEx	MM_MEST.
Genevestigator	Q07646.
Family and domain databases
InterPro	IPR000639. Epox_hydrolase-like. [Graphical view]
PRINTS	PR00412. EPOXHYDRLASE.
ProtoNet	Search...
Other
ChiTaRS	MEST. mouse.
NextBio	291822.
SOURCE	Search...

Entry information

Entry name

MEST_MOUSE

Accession

Primary (citable) accession number: Q07646
Secondary accession number(s): Q792T8 , Q8BS88, Q8K463, Q99KT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program