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Q07646 (MEST_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mesoderm-specific transcript protein

EC=3.-.-.-
Alternative name(s):
Paternally-expressed gene 1 protein
Gene names
Name:Mest
Synonyms:Peg1
ORF Names:121a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.7.

Tissue specificity

Expressed in mesodermal tissues. Isoform 1 is exclusively expressed from the paternal allele in all fetal tissues and cell lines examined, whereas isoform 2 is preferentially expressed from the paternal allele in a tissue-type-specific manner. Ref.6

Developmental stage

Expressed in the nascent mesoderm of gastrulating embryos. At 7.0 dpc expression detected in both the intraembryonic mesoderm and the extraembryonic mesoderm cells of the amniotic fold. At 8.5 dpc expressed predominantly in the head mesenchyme, allantois, and the mesodermal layer of the amnion, chorion and yolk. In 9.5 dpc embryos highly expressed in the mesenchymal tissues, presomitic paraxial mesoderm, heart and branchial arches. Ref.1

Sequence similarities

Belongs to the AB hydrolase superfamily.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q07646-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q07646-2)

Also known as: MEST intronic transcript 1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Mesoderm-specific transcript protein
PRO_0000284419

Regions

Transmembrane13 – 3321Helical; Potential
Transmembrane63 – 8321Helical; Potential
Transmembrane266 – 28621Helical; Potential
Motif98 – 1036RVIALD

Amino acid modifications

Glycosylation1631N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 99Missing in isoform 2.
VSP_024534

Experimental info

Sequence conflict431W → C in BAC28891. Ref.3
Sequence conflict1711K → N in AAM78507. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F5F394F46152E712

FASTA33538,907
        10         20         30         40         50         60 
MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKTSGKFF TYKGLRIFYQ 

        70         80         90        100        110        120 
DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI ALDFLGFGFS DKPRPHQYSI 

       130        140        150        160        170        180 
FEQASIVESL LRHLGLQNRR INLLSHDYGD IVAQELLYRY KQNRSGRLTI KSLCLSNGGI 

       190        200        210        220        230        240 
FPETHRPLLL QKLLKDGGVL SPILTRLMNF FVFSRGLTPV FGPYTRPTES ELWDMWAVIR 

       250        260        270        280        290        300 
NNDGNLVIDS LLQYINQRKK FRRRWVGALA SVSIPIHFIY GPLDPINPYP EFLELYRKTL 

       310        320        330 
PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF 

« Hide

Isoform 2 (MEST intronic transcript 1) [UniParc].

Checksum: 1C4DD363B5D3421A
Show »

FASTA32637,668

References

« Hide 'large scale' references
[1]"A novel mesoderm-specific cDNA isolated from a mouse embryonal carcinoma cell line."
Sado T., Nakajima N., Tada M., Takagi N.
Dev. Growth Differ. 35:551-560(1993)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
[2]"Identification and characterization of an imprinted antisense RNA (MESTIT1) in the human MEST locus on chromosome 7q32."
Nakabayashi K., Bentley L., Hitchins M.P., Mitsuya K., Meguro M., Minagawa S., Bamforth J.S., Stanier P., Preece M., Weksberg R., Oshimura M., Moore G.E., Scherer S.W.
Hum. Mol. Genet. 11:1743-1756(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
Strain: C57BL/6J.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Amnion, Embryo, Heart, Kidney and Wolffian duct.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Czech II.
Tissue: Mammary tumor.
[5]"Genomic structure and parent-of-origin-specific methylation of Peg1."
Lefebvre L., Viville S., Barton S.C., Ishino F., Surani M.A.
Hum. Mol. Genet. 6:1907-1915(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-60.
Strain: 129/Sv.
[6]"Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization."
Kaneko-Ishino T., Kuroiwa Y., Miyoshi N., Kohda T., Suzuki R., Yokoyama M., Viville S., Barton S.C., Ishino F., Surani M.A.
Nat. Genet. 11:52-59(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
Strain: 129/Sv.
[7]"Mesoderm-specific transcript is associated with fat mass expansion in response to a positive energy balance."
Nikonova L., Koza R.A., Mendoza T., Chao P.-M., Curley J.P., Kozak L.P.
FASEB J. 22:3925-3937(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16262 mRNA. Translation: BAA03795.1.
AF482999 mRNA. Translation: AAM78507.1.
AK032881 mRNA. Translation: BAC28068.1.
AK034949 mRNA. Translation: BAC28891.1.
AK168714 mRNA. Translation: BAE40556.1.
AK168732 mRNA. Translation: BAE40573.1.
AK168743 mRNA. Translation: BAE40584.1.
AK168995 mRNA. Translation: BAE40794.1.
AK169095 mRNA. Translation: BAE40878.1.
AK169266 mRNA. Translation: BAE41027.1.
BC004019 mRNA. Translation: AAH04019.1.
BC006639 mRNA. Translation: AAH06639.1.
AF017994 Genomic DNA. Translation: AAC53396.1.
CCDSCCDS19979.1. [Q07646-1]
RefSeqNP_001239221.1. NM_001252292.1.
NP_001239222.1. NM_001252293.1. [Q07646-2]
NP_032616.1. NM_008590.2. [Q07646-1]
UniGeneMm.335639.

3D structure databases

ProteinModelPortalQ07646.
SMRQ07646. Positions 48-333.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS33.972.

Proteomic databases

PaxDbQ07646.
PRIDEQ07646.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000163949; ENSMUSP00000129639; ENSMUSG00000051855. [Q07646-1]
GeneID17294.
KEGGmmu:17294.
UCSCuc009bfs.2. mouse. [Q07646-1]

Organism-specific databases

CTD4232.
MGIMGI:96968. Mest.

Phylogenomic databases

eggNOGCOG0596.
GeneTreeENSGT00510000047602.
HOGENOMHOG000049677.
HOVERGENHBG052816.
InParanoidQ07646.
OMASVSIPIH.
PhylomeDBQ07646.
TreeFamTF329307.

Gene expression databases

ArrayExpressQ07646.
BgeeQ07646.
CleanExMM_MEST.
GenevestigatorQ07646.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR000639. Epox_hydrolase-like.
[Graphical view]
PRINTSPR00412. EPOXHYDRLASE.
SUPFAMSSF53474. SSF53474. 1 hit.
ProtoNetSearch...

Other

ChiTaRSMEST. mouse.
NextBio291822.
PROQ07646.
SOURCESearch...

Entry information

Entry nameMEST_MOUSE
AccessionPrimary (citable) accession number: Q07646
Secondary accession number(s): Q792T8 expand/collapse secondary AC list , Q8BS88, Q8K463, Q99KT5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot