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Q07637 (KPYK_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pyk
Ordered Locus Names:LL1332
ORF Names:L0003
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Enzyme regulation

Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Pyruvate kinase
PRO_0000112077

Sites

Metal binding561Potassium By similarity
Metal binding581Potassium By similarity
Metal binding881Potassium By similarity
Metal binding891Potassium; via carbonyl oxygen By similarity
Metal binding2521Magnesium By similarity
Metal binding2761Magnesium By similarity
Binding site541Substrate By similarity
Binding site2751Substrate; via amide nitrogen By similarity
Binding site2761Substrate; via amide nitrogen By similarity
Binding site3081Substrate By similarity
Site2501Transition state stabilizer By similarity

Experimental info

Sequence conflict1001A → T in AAA99895. Ref.1
Sequence conflict1051A → S in AAA99895. Ref.1
Sequence conflict4021I → V in AAA99895. Ref.1
Sequence conflict4241N → D in AAA99895. Ref.1
Sequence conflict4491T → M in AAA99895. Ref.1
Sequence conflict4551S → A in AAA99895. Ref.1
Sequence conflict4761S → A in AAA99895. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q07637 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 5A63C790B27E6B73

FASTA50254,255
        10         20         30         40         50         60 
MNKRVKIVST LGPAVEIRGG KKFGESGYWG ESLDVEASAK NIAALIEEGA NVFRFNFSHG 

        70         80         90        100        110        120 
DHPEQGARMA TVHRAEEIAG HKVGFLLDTK GPEMRTELFA DGADAISVVT GDKFRVATKQ 

       130        140        150        160        170        180 
GLKSTPELIA LNVAGGLDIF DDVEIGQTIL IDDGKLGLSL TGKDAATREF EVEAQNDGVI 

       190        200        210        220        230        240 
GKQKGVNIPN TKIPFPALAE RDDADIRFGL SQPGGINFIA ISFVRTANDV KEVRRICEET 

       250        260        270        280        290        300 
GNPHVQLLAK IENQQGIENL DEIIEAADGI MIARGDMGIE VPFEMVPVYQ KLIISKVNKA 

       310        320        330        340        350        360 
GKIVVTATNM LESMTYNPRA TRSEISDVFN AVIDGTDATM LSGESANGKY PRESVRTMAT 

       370        380        390        400        410        420 
VNKNAQTMLK EYGRLHPERY DKSTVTEVVA ASVKNAAEAM DIKLIVALTE SGNTARLISK 

       430        440        450        460        470        480 
HRPNADILAI TFDEKVERGL MINWGVIPTM TEKPSSTDDM FEVAEKVALA SGLVESGDNI 

       490        500 
IIVAGVPVGT GRTNTMRIRT VK 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase."
Llanos R.M., Harris C.J., Hillier A.J., Davidson B.E.
J. Bacteriol. 175:2541-2551(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LM0230.
[2]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07920 Genomic DNA. Translation: AAA99895.1.
AE005176 Genomic DNA. Translation: AAK05430.1.
PIRB40620.
D86791.
RefSeqNP_267488.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ07637.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L0003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK05430; AAK05430; L0003.
GeneID1114982.
KEGGlla:L0003.
PATRIC22295062. VBILacLac136773_1439.

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
KOK00873.
OMAGTHEEHK.
OrthoDBEOG6GBMB0.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-1421-MONOMER.
MetaCyc:MONOMER-13043.
SABIO-RKQ07637.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_LACLA
AccessionPrimary (citable) accession number: Q07637
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 27, 2001
Last modified: June 11, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways