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Q07637

- KPYK_LACLA

UniProt

Q07637 - KPYK_LACLA

Protein

Pyruvate kinase

Gene

pyk

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (27 Apr 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Enzyme regulationi

    Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541SubstrateBy similarity
    Metal bindingi56 – 561PotassiumBy similarity
    Metal bindingi58 – 581PotassiumBy similarity
    Metal bindingi88 – 881PotassiumBy similarity
    Metal bindingi89 – 891Potassium; via carbonyl oxygenBy similarity
    Sitei250 – 2501Transition state stabilizerBy similarity
    Metal bindingi252 – 2521MagnesiumBy similarity
    Binding sitei275 – 2751Substrate; via amide nitrogenBy similarity
    Metal bindingi276 – 2761MagnesiumBy similarity
    Binding sitei276 – 2761Substrate; via amide nitrogenBy similarity
    Binding sitei308 – 3081SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciLLAC272623:GHSH-1421-MONOMER.
    MetaCyc:MONOMER-13043.
    SABIO-RKQ07637.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    Ordered Locus Names:LL1332
    ORF Names:L0003
    OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
    Taxonomic identifieri272623 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000002196: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502Pyruvate kinasePRO_0000112077Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi272623.L0003.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07637.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    KOiK00873.
    OMAiGTHEEHK.
    OrthoDBiEOG6GBMB0.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q07637-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKRVKIVST LGPAVEIRGG KKFGESGYWG ESLDVEASAK NIAALIEEGA    50
    NVFRFNFSHG DHPEQGARMA TVHRAEEIAG HKVGFLLDTK GPEMRTELFA 100
    DGADAISVVT GDKFRVATKQ GLKSTPELIA LNVAGGLDIF DDVEIGQTIL 150
    IDDGKLGLSL TGKDAATREF EVEAQNDGVI GKQKGVNIPN TKIPFPALAE 200
    RDDADIRFGL SQPGGINFIA ISFVRTANDV KEVRRICEET GNPHVQLLAK 250
    IENQQGIENL DEIIEAADGI MIARGDMGIE VPFEMVPVYQ KLIISKVNKA 300
    GKIVVTATNM LESMTYNPRA TRSEISDVFN AVIDGTDATM LSGESANGKY 350
    PRESVRTMAT VNKNAQTMLK EYGRLHPERY DKSTVTEVVA ASVKNAAEAM 400
    DIKLIVALTE SGNTARLISK HRPNADILAI TFDEKVERGL MINWGVIPTM 450
    TEKPSSTDDM FEVAEKVALA SGLVESGDNI IIVAGVPVGT GRTNTMRIRT 500
    VK 502
    Length:502
    Mass (Da):54,255
    Last modified:April 27, 2001 - v2
    Checksum:i5A63C790B27E6B73
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti100 – 1001A → T in AAA99895. (PubMed:8478320)Curated
    Sequence conflicti105 – 1051A → S in AAA99895. (PubMed:8478320)Curated
    Sequence conflicti402 – 4021I → V in AAA99895. (PubMed:8478320)Curated
    Sequence conflicti424 – 4241N → D in AAA99895. (PubMed:8478320)Curated
    Sequence conflicti449 – 4491T → M in AAA99895. (PubMed:8478320)Curated
    Sequence conflicti455 – 4551S → A in AAA99895. (PubMed:8478320)Curated
    Sequence conflicti476 – 4761S → A in AAA99895. (PubMed:8478320)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07920 Genomic DNA. Translation: AAA99895.1.
    AE005176 Genomic DNA. Translation: AAK05430.1.
    PIRiB40620.
    D86791.
    RefSeqiNP_267488.1. NC_002662.1.

    Genome annotation databases

    EnsemblBacteriaiAAK05430; AAK05430; L0003.
    GeneIDi1114982.
    KEGGilla:L0003.
    PATRICi22295062. VBILacLac136773_1439.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07920 Genomic DNA. Translation: AAA99895.1 .
    AE005176 Genomic DNA. Translation: AAK05430.1 .
    PIRi B40620.
    D86791.
    RefSeqi NP_267488.1. NC_002662.1.

    3D structure databases

    ProteinModelPortali Q07637.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272623.L0003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK05430 ; AAK05430 ; L0003 .
    GeneIDi 1114982.
    KEGGi lla:L0003.
    PATRICi 22295062. VBILacLac136773_1439.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    KOi K00873.
    OMAi GTHEEHK.
    OrthoDBi EOG6GBMB0.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci LLAC272623:GHSH-1421-MONOMER.
    MetaCyc:MONOMER-13043.
    SABIO-RK Q07637.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase."
      Llanos R.M., Harris C.J., Hillier A.J., Davidson B.E.
      J. Bacteriol. 175:2541-2551(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LM0230.
    2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
      Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
      Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IL1403.

    Entry informationi

    Entry nameiKPYK_LACLA
    AccessioniPrimary (citable) accession number: Q07637
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3