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Q07637

- KPYK_LACLA

UniProt

Q07637 - KPYK_LACLA

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Protein

Pyruvate kinase

Gene

pyk

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Enzyme regulationi

Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541SubstrateBy similarity
Metal bindingi56 – 561PotassiumBy similarity
Metal bindingi58 – 581PotassiumBy similarity
Metal bindingi88 – 881PotassiumBy similarity
Metal bindingi89 – 891Potassium; via carbonyl oxygenBy similarity
Sitei250 – 2501Transition state stabilizerBy similarity
Metal bindingi252 – 2521MagnesiumBy similarity
Binding sitei275 – 2751Substrate; via amide nitrogenBy similarity
Metal bindingi276 – 2761MagnesiumBy similarity
Binding sitei276 – 2761Substrate; via amide nitrogenBy similarity
Binding sitei308 – 3081SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1421-MONOMER.
MetaCyc:MONOMER-13043.
SABIO-RKQ07637.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:LL1332
ORF Names:L0003
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Pyruvate kinasePRO_0000112077Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272623.L0003.

Structurei

3D structure databases

ProteinModelPortaliQ07637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
KOiK00873.
OMAiGTHEEHK.
OrthoDBiEOG6GBMB0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07637-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKRVKIVST LGPAVEIRGG KKFGESGYWG ESLDVEASAK NIAALIEEGA
60 70 80 90 100
NVFRFNFSHG DHPEQGARMA TVHRAEEIAG HKVGFLLDTK GPEMRTELFA
110 120 130 140 150
DGADAISVVT GDKFRVATKQ GLKSTPELIA LNVAGGLDIF DDVEIGQTIL
160 170 180 190 200
IDDGKLGLSL TGKDAATREF EVEAQNDGVI GKQKGVNIPN TKIPFPALAE
210 220 230 240 250
RDDADIRFGL SQPGGINFIA ISFVRTANDV KEVRRICEET GNPHVQLLAK
260 270 280 290 300
IENQQGIENL DEIIEAADGI MIARGDMGIE VPFEMVPVYQ KLIISKVNKA
310 320 330 340 350
GKIVVTATNM LESMTYNPRA TRSEISDVFN AVIDGTDATM LSGESANGKY
360 370 380 390 400
PRESVRTMAT VNKNAQTMLK EYGRLHPERY DKSTVTEVVA ASVKNAAEAM
410 420 430 440 450
DIKLIVALTE SGNTARLISK HRPNADILAI TFDEKVERGL MINWGVIPTM
460 470 480 490 500
TEKPSSTDDM FEVAEKVALA SGLVESGDNI IIVAGVPVGT GRTNTMRIRT

VK
Length:502
Mass (Da):54,255
Last modified:April 27, 2001 - v2
Checksum:i5A63C790B27E6B73
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001A → T in AAA99895. (PubMed:8478320)Curated
Sequence conflicti105 – 1051A → S in AAA99895. (PubMed:8478320)Curated
Sequence conflicti402 – 4021I → V in AAA99895. (PubMed:8478320)Curated
Sequence conflicti424 – 4241N → D in AAA99895. (PubMed:8478320)Curated
Sequence conflicti449 – 4491T → M in AAA99895. (PubMed:8478320)Curated
Sequence conflicti455 – 4551S → A in AAA99895. (PubMed:8478320)Curated
Sequence conflicti476 – 4761S → A in AAA99895. (PubMed:8478320)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07920 Genomic DNA. Translation: AAA99895.1.
AE005176 Genomic DNA. Translation: AAK05430.1.
PIRiB40620.
D86791.
RefSeqiNP_267488.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05430; AAK05430; L0003.
GeneIDi1114982.
KEGGilla:L0003.
PATRICi22295062. VBILacLac136773_1439.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07920 Genomic DNA. Translation: AAA99895.1 .
AE005176 Genomic DNA. Translation: AAK05430.1 .
PIRi B40620.
D86791.
RefSeqi NP_267488.1. NC_002662.1.

3D structure databases

ProteinModelPortali Q07637.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272623.L0003.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK05430 ; AAK05430 ; L0003 .
GeneIDi 1114982.
KEGGi lla:L0003.
PATRICi 22295062. VBILacLac136773_1439.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
KOi K00873.
OMAi GTHEEHK.
OrthoDBi EOG6GBMB0.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BioCyci LLAC272623:GHSH-1421-MONOMER.
MetaCyc:MONOMER-13043.
SABIO-RK Q07637.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase."
    Llanos R.M., Harris C.J., Hillier A.J., Davidson B.E.
    J. Bacteriol. 175:2541-2551(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LM0230.
  2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiKPYK_LACLA
AccessioniPrimary (citable) accession number: Q07637
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 27, 2001
Last modified: October 1, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3