Reviewed, UniProtKB/Swiss-Prot Q07637 (KPYK_LACLA)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:	pyk
Ordered Locus Names:	LL1332
ORF Names:	L0003

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP]

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Enzyme regulation	Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Pyruvate kinase		PRO_0000112077
Sites
	Active site	250	1	By similarity
	Metal binding	252	1	Magnesium By similarity
	Metal binding	273	1	Magnesium By similarity
	Metal binding	274	1	Magnesium By similarity
Experimental info
	Sequence conflict	100	1	A → T in AAA99895. Ref.1
	Sequence conflict	105	1	A → S in AAA99895. Ref.1
	Sequence conflict	402	1	I → V in AAA99895. Ref.1
	Sequence conflict	424	1	N → D in AAA99895. Ref.1
	Sequence conflict	449	1	T → M in AAA99895. Ref.1
	Sequence conflict	455	1	S → A in AAA99895. Ref.1
	Sequence conflict	476	1	S → A in AAA99895. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q07637-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 5A63C790B27E6B73	FASTA	502	54,255
	10 20 30 40 50 60 MNKRVKIVST LGPAVEIRGG KKFGESGYWG ESLDVEASAK NIAALIEEGA NVFRFNFSHG 70 80 90 100 110 120 DHPEQGARMA TVHRAEEIAG HKVGFLLDTK GPEMRTELFA DGADAISVVT GDKFRVATKQ 130 140 150 160 170 180 GLKSTPELIA LNVAGGLDIF DDVEIGQTIL IDDGKLGLSL TGKDAATREF EVEAQNDGVI 190 200 210 220 230 240 GKQKGVNIPN TKIPFPALAE RDDADIRFGL SQPGGINFIA ISFVRTANDV KEVRRICEET 250 260 270 280 290 300 GNPHVQLLAK IENQQGIENL DEIIEAADGI MIARGDMGIE VPFEMVPVYQ KLIISKVNKA 310 320 330 340 350 360 GKIVVTATNM LESMTYNPRA TRSEISDVFN AVIDGTDATM LSGESANGKY PRESVRTMAT 370 380 390 400 410 420 VNKNAQTMLK EYGRLHPERY DKSTVTEVVA ASVKNAAEAM DIKLIVALTE SGNTARLISK 430 440 450 460 470 480 HRPNADILAI TFDEKVERGL MINWGVIPTM TEKPSSTDDM FEVAEKVALA SGLVESGDNI 490 500 IIVAGVPVGT GRTNTMRIRT VK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase." Llanos R.M., Harris C.J., Hillier A.J., Davidson B.E. J. Bacteriol. 175:2541-2551(1993) [PubMed: 8478320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LM0230.
[2]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L07920 Genomic DNA. Translation: AAA99895.1. AE006365 Genomic DNA. Translation: AAK05430.1.
PIR	B40620. D86791.
RefSeq	NP_267488.1.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Genome annotation databases
GeneID	1114982.
GenomeReviews	Gene locus LL1332 in contig AE005176_GR.
KEGG	lla:L0003.
NMPDR	fig|272623.1.peg.1367.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q07637.
OMA	Q07637. WVSKSQR.
Enzyme and pathway databases
BioCyc	LLAC272623:L0003-MON. MetaCyc:MON-13043.
BRENDA	2.7.1.40. 278870.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_LACLA

Accession

Primary (citable) accession number: Q07637

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	April 27, 2001
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)