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Reviewed, UniProtKB/Swiss-Prot Q07637 (KPYK_LACLA)

Last modified June 16, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40
Gene names
Name: pyk
Ordered Locus Names: LL1332
ORF Names: L0003
OrganismLactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP]
Taxonomic identifier1360 [NCBI]
Taxonomic lineageBacteriaFirmicutesLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Enzyme regulation

Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Pyruvate kinase
PRO_0000112077

Sites

Active site2501 By similarity
Metal binding2521Magnesium By similarity
Metal binding2731Magnesium By similarity
Metal binding2741Magnesium By similarity

Experimental info

Sequence conflict1001A → T in AAA99895. Ref.1
Sequence conflict1051A → S in AAA99895. Ref.1
Sequence conflict4021I → V in AAA99895. Ref.1
Sequence conflict4241N → D in AAA99895. Ref.1
Sequence conflict4491T → M in AAA99895. Ref.1
Sequence conflict4551S → A in AAA99895. Ref.1
Sequence conflict4761S → A in AAA99895. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q07637-1 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 5A63C790B27E6B73

FASTA50254,255
        10         20         30         40         50         60 
MNKRVKIVST LGPAVEIRGG KKFGESGYWG ESLDVEASAK NIAALIEEGA NVFRFNFSHG 

        70         80         90        100        110        120 
DHPEQGARMA TVHRAEEIAG HKVGFLLDTK GPEMRTELFA DGADAISVVT GDKFRVATKQ 

       130        140        150        160        170        180 
GLKSTPELIA LNVAGGLDIF DDVEIGQTIL IDDGKLGLSL TGKDAATREF EVEAQNDGVI 

       190        200        210        220        230        240 
GKQKGVNIPN TKIPFPALAE RDDADIRFGL SQPGGINFIA ISFVRTANDV KEVRRICEET 

       250        260        270        280        290        300 
GNPHVQLLAK IENQQGIENL DEIIEAADGI MIARGDMGIE VPFEMVPVYQ KLIISKVNKA 

       310        320        330        340        350        360 
GKIVVTATNM LESMTYNPRA TRSEISDVFN AVIDGTDATM LSGESANGKY PRESVRTMAT 

       370        380        390        400        410        420 
VNKNAQTMLK EYGRLHPERY DKSTVTEVVA ASVKNAAEAM DIKLIVALTE SGNTARLISK 

       430        440        450        460        470        480 
HRPNADILAI TFDEKVERGL MINWGVIPTM TEKPSSTDDM FEVAEKVALA SGLVESGDNI 

       490        500 
IIVAGVPVGT GRTNTMRIRT VK 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase."
Llanos R.M., Harris C.J., Hillier A.J., Davidson B.E.
J. Bacteriol. 175:2541-2551(1993) [PubMed: 8478320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LM0230.
[2]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

L07920 Genomic DNA. Translation: AAA99895.1.
AE006365 Genomic DNA. Translation: AAK05430.1.
PIRB40620.
D86791.
RefSeqNP_267488.1.

3D structure databases

HSSPHSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBaseSearch...

Genome annotation databases

GeneID1114982.
GenomeReviewsGene locus LL1332 in contig AE005176_GR.
KEGGlla:L0003.
NMPDRfig|272623.1.peg.1367.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ07637.
OMAQ07637. WVSKSQR.

Enzyme and pathway databases

BioCycLLAC272623:L0003-MON.
MetaCyc:MON-13043.
BRENDA2.7.1.40. 278870.

Family and domain databases

InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase_cat.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
[Graphical view]
Gene3DG3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHERPTHR11817. Pyruvate_kinase. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
ProDomPD001009. Pyruvate_kinase. 2 hits.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_LACLA
AccessionPrimary (citable) accession number: Q07637
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 27, 2001
Last modified: June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents