Pyruvate kinase - Lactococcus lactis subsp. lactis (strain IL1403)

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Q07637 (KPYK_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 99. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:	pyk
Ordered Locus Names:	LL1332
ORF Names:	L0003

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus ›

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Enzyme regulation	Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 502

502

Pyruvate kinase

PRO_0000112077

Sites

Metal binding

Potassium By similarity

Metal binding

Potassium By similarity

Metal binding

Potassium By similarity

Metal binding

Potassium; via carbonyl oxygen By similarity

Metal binding

252

Magnesium By similarity

Metal binding

276

Magnesium By similarity

Binding site

Substrate By similarity

Binding site

275

Substrate; via amide nitrogen By similarity

Binding site

276

Substrate; via amide nitrogen By similarity

Binding site

308

Substrate By similarity

Site

250

Transition state stabilizer By similarity

Experimental info

Sequence conflict

100

A → T in AAA99895. Ref.1

Sequence conflict

105

A → S in AAA99895. Ref.1

Sequence conflict

402

I → V in AAA99895. Ref.1

Sequence conflict

424

N → D in AAA99895. Ref.1

Sequence conflict

449

T → M in AAA99895. Ref.1

Sequence conflict

455

S → A in AAA99895. Ref.1

Sequence conflict

476

S → A in AAA99895. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q07637 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 5A63C790B27E6B73
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FASTA

502

54,255

        10         20         30         40         50         60 
MNKRVKIVST LGPAVEIRGG KKFGESGYWG ESLDVEASAK NIAALIEEGA NVFRFNFSHG 

        70         80         90        100        110        120 
DHPEQGARMA TVHRAEEIAG HKVGFLLDTK GPEMRTELFA DGADAISVVT GDKFRVATKQ 

       130        140        150        160        170        180 
GLKSTPELIA LNVAGGLDIF DDVEIGQTIL IDDGKLGLSL TGKDAATREF EVEAQNDGVI 

       190        200        210        220        230        240 
GKQKGVNIPN TKIPFPALAE RDDADIRFGL SQPGGINFIA ISFVRTANDV KEVRRICEET 

       250        260        270        280        290        300 
GNPHVQLLAK IENQQGIENL DEIIEAADGI MIARGDMGIE VPFEMVPVYQ KLIISKVNKA 

       310        320        330        340        350        360 
GKIVVTATNM LESMTYNPRA TRSEISDVFN AVIDGTDATM LSGESANGKY PRESVRTMAT 

       370        380        390        400        410        420 
VNKNAQTMLK EYGRLHPERY DKSTVTEVVA ASVKNAAEAM DIKLIVALTE SGNTARLISK 

       430        440        450        460        470        480 
HRPNADILAI TFDEKVERGL MINWGVIPTM TEKPSSTDDM FEVAEKVALA SGLVESGDNI 

       490        500 
IIVAGVPVGT GRTNTMRIRT VK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase." Llanos R.M., Harris C.J., Hillier A.J., Davidson B.E. J. Bacteriol. 175:2541-2551(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LM0230.
[2]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07920 Genomic DNA. Translation: AAA99895.1. AE005176 Genomic DNA. Translation: AAK05430.1.
PIR	B40620. D86791.
RefSeq	NP_267488.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q07637.
ModBase	Search...
Protein-protein interaction databases
STRING	272623.L0003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK05430; AAK05430; L0003.
GeneID	1114982.
KEGG	lla:L0003.
PATRIC	22295062. VBILacLac136773_1439.
Phylogenomic databases
eggNOG	COG0469.
HOGENOM	HOG000021559.
KO	K00873.
OMA	ALHRIGT.
ProtClustDB	PRK05826.
Enzyme and pathway databases
BioCyc	LLAC272623:GHSH-1421-MONOMER. MetaCyc:MONOMER-13043.
SABIO-RK	Q07637.
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_LACLA

Accession

Primary (citable) accession number: Q07637

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	April 27, 2001
Last modified:	May 29, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents