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Protein

Pyruvate kinase

Gene

pyk

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase 2 (eno2), Enolase 1 (eno1)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54SubstrateBy similarity1
Metal bindingi56PotassiumBy similarity1
Metal bindingi58PotassiumBy similarity1
Metal bindingi88PotassiumBy similarity1
Metal bindingi89Potassium; via carbonyl oxygenBy similarity1
Sitei250Transition state stabilizerBy similarity1
Metal bindingi252MagnesiumBy similarity1
Binding sitei275Substrate; via amide nitrogenBy similarity1
Metal bindingi276MagnesiumBy similarity1
Binding sitei276Substrate; via amide nitrogenBy similarity1
Binding sitei308SubstrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • mannan binding Source: CAFA
  • potassium ion binding Source: InterPro
  • pyruvate kinase activity Source: CAFA

GO - Biological processi

  • NADH oxidation Source: CAFA

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciLLAC272623:G1FZA-1409-MONOMER
MetaCyc:MONOMER-13043
SABIO-RKQ07637
UniPathwayiUPA00109; UER00188

Protein family/group databases

MoonProtiQ07637

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:LL1332
ORF Names:L0003
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: CAFA

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120771 – 502Pyruvate kinaseAdd BLAST502

Proteomic databases

PaxDbiQ07637
PRIDEiQ07637

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272623.L0003

Structurei

3D structure databases

ProteinModelPortaliQ07637
SMRiQ07637
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9 Bacteria
COG0469 LUCA
HOGENOMiHOG000021559
KOiK00873
OMAiKHEAIEQ

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q07637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKRVKIVST LGPAVEIRGG KKFGESGYWG ESLDVEASAK NIAALIEEGA
60 70 80 90 100
NVFRFNFSHG DHPEQGARMA TVHRAEEIAG HKVGFLLDTK GPEMRTELFA
110 120 130 140 150
DGADAISVVT GDKFRVATKQ GLKSTPELIA LNVAGGLDIF DDVEIGQTIL
160 170 180 190 200
IDDGKLGLSL TGKDAATREF EVEAQNDGVI GKQKGVNIPN TKIPFPALAE
210 220 230 240 250
RDDADIRFGL SQPGGINFIA ISFVRTANDV KEVRRICEET GNPHVQLLAK
260 270 280 290 300
IENQQGIENL DEIIEAADGI MIARGDMGIE VPFEMVPVYQ KLIISKVNKA
310 320 330 340 350
GKIVVTATNM LESMTYNPRA TRSEISDVFN AVIDGTDATM LSGESANGKY
360 370 380 390 400
PRESVRTMAT VNKNAQTMLK EYGRLHPERY DKSTVTEVVA ASVKNAAEAM
410 420 430 440 450
DIKLIVALTE SGNTARLISK HRPNADILAI TFDEKVERGL MINWGVIPTM
460 470 480 490 500
TEKPSSTDDM FEVAEKVALA SGLVESGDNI IIVAGVPVGT GRTNTMRIRT

VK
Length:502
Mass (Da):54,255
Last modified:April 27, 2001 - v2
Checksum:i5A63C790B27E6B73
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100A → T in AAA99895 (PubMed:8478320).Curated1
Sequence conflicti105A → S in AAA99895 (PubMed:8478320).Curated1
Sequence conflicti402I → V in AAA99895 (PubMed:8478320).Curated1
Sequence conflicti424N → D in AAA99895 (PubMed:8478320).Curated1
Sequence conflicti449T → M in AAA99895 (PubMed:8478320).Curated1
Sequence conflicti455S → A in AAA99895 (PubMed:8478320).Curated1
Sequence conflicti476S → A in AAA99895 (PubMed:8478320).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07920 Genomic DNA Translation: AAA99895.1
AE005176 Genomic DNA Translation: AAK05430.1
PIRiB40620
D86791
RefSeqiNP_267488.1, NC_002662.1
WP_003131076.1, NC_002662.1

Genome annotation databases

EnsemblBacteriaiAAK05430; AAK05430; L0003
GeneIDi1114982
KEGGilla:L0003
PATRICifig|272623.7.peg.1439

Similar proteinsi

Entry informationi

Entry nameiKPYK_LACLA
AccessioniPrimary (citable) accession number: Q07637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 27, 2001
Last modified: May 23, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health