Q07636 (K6PF_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Synonyms:	pfk
Ordered Locus Names:	LL1333
ORF Names:	L0002

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. lactis

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Enzyme regulation	Activated by ADP and inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_0000111958
Regions
	Nucleotide binding	21 – 25	5	ATP By similarity
	Nucleotide binding	154 – 158	5	ATP By similarity
	Nucleotide binding	171 – 187	17	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Metal binding	185	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Binding site	253	1	Substrate By similarity
Experimental info
	Sequence conflict	190	1	C → S AA sequence Ref.1
	Sequence conflict	194	1	K → L AA sequence Ref.1
	Sequence conflict	219 – 220	2	VL → IV AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q07636 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: F1BB2BD3FD3661B7	FASTA	340	35,806
	10 20 30 40 50 60 MKRIAVLTSG GDAPGMNAAI RAVVRKAISE GIEVYGINHG YAGMVAGDIF PLTSASVGDK 70 80 90 100 110 120 IGRGGTFLYS ARYPEFAQVE GQLAGIEQLK KFGIEGVVVI GGDGSYHGAM RLTEHGFPAV 130 140 150 160 170 180 GLPGTIDNDI VGTDFTIGFD TAVSTVVDAL DKIRDTSSSH NRTFVVEVMG RNAGDIALNA 190 200 210 220 230 240 GIAAGADDIC IPEKEFKFEN VVNNINKGYE KGKNHHIIVL AEGVMTGEEF ATKLKEAGYK 250 260 270 280 290 300 GDLRVSVLGH IQRGGSPTAR DRVLASRMGA RAVELLRDGI GGVAVGIRNE ELVESPILGT 310 320 330 340 AEEGALFSLT TEGGIKVNNP HKAGLELYRL NSALNNLNLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of phosphofructokinase from Lactococcus lactis." Xiao Q., Moore C.H. Biochem. Biophys. Res. Commun. 194:65-71(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: C10.
[2]	"Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase." Llanos R.M., Harris C.J., Hillier A.J., Davidson B.E. J. Bacteriol. 175:2541-2551(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LM0230.
[3]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07920 Genomic DNA. Translation: AAA99894.1. AE005176 Genomic DNA. Translation: AAK05431.1.
PIR	JN0614. A40620.
RefSeq	NP_267489.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q07636.
SMR	Q07636. Positions 1-297.
ModBase	Search...
Protein-protein interaction databases
STRING	272623.L0002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK05431; AAK05431; L0002.
GeneID	1114983.
KEGG	lla:L0002.
PATRIC	22295064. VBILacLac136773_1440.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248870.
KO	K00850.
OMA	EFKTVEG.
ProtClustDB	PRK03202.
Enzyme and pathway databases
BioCyc	LLAC272623:GHSH-1463-MONOMER. MetaCyc:MONOMER-13044.
SABIO-RK	Q07636.
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_LACLA

Accession

Primary (citable) accession number: Q07636

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program