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Q07636 (PFKA_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Synonyms:pfk
Ordered Locus Names:LL1333
ORF Names:L0002
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Ref.4

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. Ref.4

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer. Ref.4

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=0.18 mM for ATP Ref.4

KM=0.25 mM for fructose 6-phosphate

pH dependence:

Optimum pH is 7.2-7.8.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339
PRO_0000111958

Regions

Nucleotide binding72 – 732ATP By similarity
Nucleotide binding102 – 1054ATP By similarity
Region21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarity
Region125 – 1273Substrate binding By similarity
Region169 – 1713Substrate binding By similarity
Region185 – 1873Allosteric activator ADP binding By similarity
Region213 – 2153Allosteric activator ADP binding By similarity
Region250 – 2534Substrate binding By similarity

Sites

Active site1271Proton acceptor By similarity
Metal binding1031Magnesium; catalytic By similarity
Binding site111ATP; via amide nitrogen By similarity
Binding site1541Allosteric activator ADP By similarity
Binding site1621Substrate; shared with dimeric partner By similarity
Binding site2111Allosteric activator ADP By similarity
Binding site2221Substrate By similarity
Binding site2441Substrate; shared with dimeric partner By similarity

Experimental info

Sequence conflict1901C → S AA sequence Ref.1
Sequence conflict1941K → L AA sequence Ref.1
Sequence conflict219 – 2202VL → IV AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q07636 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: F1BB2BD3FD3661B7

FASTA34035,806
        10         20         30         40         50         60 
MKRIAVLTSG GDAPGMNAAI RAVVRKAISE GIEVYGINHG YAGMVAGDIF PLTSASVGDK 

        70         80         90        100        110        120 
IGRGGTFLYS ARYPEFAQVE GQLAGIEQLK KFGIEGVVVI GGDGSYHGAM RLTEHGFPAV 

       130        140        150        160        170        180 
GLPGTIDNDI VGTDFTIGFD TAVSTVVDAL DKIRDTSSSH NRTFVVEVMG RNAGDIALNA 

       190        200        210        220        230        240 
GIAAGADDIC IPEKEFKFEN VVNNINKGYE KGKNHHIIVL AEGVMTGEEF ATKLKEAGYK 

       250        260        270        280        290        300 
GDLRVSVLGH IQRGGSPTAR DRVLASRMGA RAVELLRDGI GGVAVGIRNE ELVESPILGT 

       310        320        330        340 
AEEGALFSLT TEGGIKVNNP HKAGLELYRL NSALNNLNLN 

« Hide

References

« Hide 'large scale' references
[1]"The primary structure of phosphofructokinase from Lactococcus lactis."
Xiao Q., Moore C.H.
Biochem. Biophys. Res. Commun. 194:65-71(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Strain: C10.
[2]"Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase."
Llanos R.M., Harris C.J., Hillier A.J., Davidson B.E.
J. Bacteriol. 175:2541-2551(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LM0230.
[3]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.
[4]"Phosphofructokinase from Streptococcus lactis."
Fordyce A.M., Moore C.H., Pritchard G.G.
Methods Enzymol. 90:77-82(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07920 Genomic DNA. Translation: AAA99894.1.
AE005176 Genomic DNA. Translation: AAK05431.1.
PIRJN0614. A40620.
RefSeqNP_267489.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ07636.
SMRQ07636. Positions 1-297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L0002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK05431; AAK05431; L0002.
GeneID1114983.
KEGGlla:L0002.
PATRIC22295064. VBILacLac136773_1440.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMAGFGGRCV.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-1422-MONOMER.
MetaCyc:MONOMER-13044.
SABIO-RKQ07636.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase_I_B1.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_LACLA
AccessionPrimary (citable) accession number: Q07636
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways