Reviewed, UniProtKB/Swiss-Prot Q07622 (ACK1_YEAST)

Names and origin

Protein names

Recommended name:
    Activator of C kinase protein 1

Gene names

Name:	ACK1
Ordered Locus Names:	YDL203C
ORF Names:	D1066

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	Present with 98 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Contains 4 Sel1-like repeats.

Ontologies

Keywords
Domain	Repeat
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from mutant phenotype. Source: SGD positive regulation of signal transduction Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P53238	1	EBI-38674,EBI-23185
NIP29	P33419	1	EBI-38674,EBI-12041
ROM2	P51862	1	EBI-38674,EBI-15702
RSP5	P39940	1	EBI-38674,EBI-16219

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	Activator of C kinase protein 1		PRO_0000242166
Regions
	Repeat	318 – 361	44	Sel1-like 1
	Repeat	408 – 444	37	Sel1-like 2
	Repeat	495 – 531	37	Sel1-like 3
	Repeat	576 – 611	36	Sel1-like 4
	Compositional bias	68 – 76	9	Poly-Ser
Amino acid modifications
	Modified residue	143	1	Phosphoserine Ref.5
	Modified residue	155	1	Phosphoserine Ref.6
	Cross-link	184		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q07622-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5CBB9D907443EC2E	FASTA	623	69,371
	10 20 30 40 50 60 MVNQGQPQPN LYDKHINMFP PARARESSHK LGNANSDRHG LPAQNIVPAP YPVDDSIVEL 70 80 90 100 110 120 TPAIPFTSPS SSSSLSLPLS ALNFTDGNAD GGQLGTPVTI NSNNGMDIFN SKPTGEIGYA 130 140 150 160 170 180 NNGTNSTGSR YELPFNFSST KESLGSPAVQ DASISSGNRI SESVRDNSAP PPYEESESRI 190 200 210 220 230 240 LQEKVYRTEE KAPIRPLNNN PVPPQKINQP PTGSAKTDDN GSSGGEDKLS SYSPEALAFY 250 260 270 280 290 300 QVYKKTITDS SKFTPEIQMQ WCETLLTYAF NEDFISQYNI NAEKLKRSLK PEEMLKNQKV 310 320 330 340 350 360 ILEHSFKVLT KLITLKWPPA MYLMGTLYSH QPYLPIKNKN IVIKNDEKAL EYYCKAAKLN 370 380 390 400 410 420 NSDACYRAGV CFEYQRGTSS LDPSPTKEQC IKKAFQYYQH GAEVCSNSAC MYKLGMSHLY 430 440 450 460 470 480 GLNMQKTDVL LAIKWFDKAA QKGDSPQTLY ELGKIYEFSV LPPEIQNLLF ANGIRKDSQL 490 500 510 520 530 540 AIKYYQQCAK DFEYPLAQWK LGNCYEFGDL GLPVVAKKSI YWYSKAAAAQ PKGNPMAMLS 550 560 570 580 590 600 LSGWYLTGAP NILKPNNKEA FNWALKSSKC SDGKLARTEF ALGFYYEKGV GCEVDLDLAK 610 620 QYYQRAARMG FRKAVDALRS LTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA." Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R. Yeast 13:163-169(1997) [PubMed: 9046097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-184, MASS SPECTROMETRY.
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99000 Genomic DNA. Translation: CAA67465.1. Z74251 Genomic DNA. Translation: CAA98781.1.
PIR	S67762.
RefSeq	NP_010078.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1805N.
IntAct	Q07622. 18 interactions.
STRING	Q07622.
Genome annotation databases
Ensembl	YDL203C; YDL203C; YDL203C; Saccharomyces cerevisiae. [Genome view]
GeneID	851324.
KEGG	sce:YDL203C.
NMPDR	fig|4932.3.peg.811.
Organism-specific databases
CYGD	YDL203c.
SGD	S000002362. ACK1.
Phylogenomic databases
HOGENOM	Q07622.
OMA	GWYLTGA
OrthoDB	EOG91VMK4
Gene expression databases
ArrayExpress	Q07622.
Genevestigator	Q07622.
GermOnline	YDL203C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006597. Sel1-like. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 2 hits.
Pfam	PF08238. Sel1. 5 hits. [Graphical view]
SMART	SM00671. SEL1. 7 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968372.

Entry information

Entry name

ACK1_YEAST

Accession

Primary (citable) accession number: Q07622

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)