Q07617 (SPAG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sperm-associated antigen 1

Alternative name(s):
HSD-3.8
Infertility-related sperm protein Spag-1

Gene names

Name:

SPAG1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	926 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in fertilization. Binds GTP and has GTPase activity. Ref.1 Ref.4
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Detected in sperm and testis, in particular in pachytene primary spermatocytes. Ref.1 Ref.4
Miscellaneous	Antibodies against SPAG1 interfere with fertilization By similarity.
Sequence similarities	Contains 9 TPR repeats.
Sequence caution	The sequence AAH55091.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Fertilization
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Repeat TPR repeat
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	single fertilization Traceable author statement Ref.4. Source: ProtInc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 926	926	Sperm-associated antigen 1		PRO_0000106324
Regions
	Repeat	209 – 242	34	TPR 1
	Repeat	244 – 275	32	TPR 2
	Repeat	276 – 309	34	TPR 3
	Repeat	445 – 478	34	TPR 4
	Repeat	487 – 520	34	TPR 5
	Repeat	522 – 554	33	TPR 6
	Repeat	623 – 656	34	TPR 7
	Repeat	657 – 690	34	TPR 8
	Repeat	692 – 724	33	TPR 9
	Nucleotide binding	781 – 788	8	GTP Potential
	Compositional bias	422 – 428	7	Poly-Ala
Amino acid modifications
	Modified residue	347	1	Phosphoserine Ref.5
	Modified residue	791	1	Phosphoserine Ref.5
Natural variations
	Natural variant	331	1	E → K. Corresponds to variant rs17335870 [ dbSNP | Ensembl ].		VAR_054324
	Natural variant	777	1	M → T. Ref.1 Corresponds to variant rs6511 [ dbSNP | Ensembl ].		VAR_054325
	Natural variant	827	1	H → Y. Corresponds to variant rs6510 [ dbSNP | Ensembl ].		VAR_054326
Experimental info
	Sequence conflict	187	1	H → R in AAG23967. Ref.1
	Sequence conflict	655	1	K → T in AAG23967. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q07617 [UniParc]. Last modified February 10, 2009. Version 3. Checksum: 947E460842AD9615	FASTA	926	103,639
	10 20 30 40 50 60 MTTKDYPSLW GFGTTKTFKI PIEHLDFKYI EKCSDVKHLE KILCVLRSGE EGYYPELTEF 70 80 90 100 110 120 CEKHLQALAP ESRALRKDKP AATAASFTAE EWEKIDGDIK SWVSEIKKEE DKMHFHETET 130 140 150 160 170 180 FPAMKDNLPP VRGSNSCLHV GKEKYSKRPT KKKTPRDYAE WDKFDVEKEC LKIDEDYKEK 190 200 210 220 230 240 TVIDKSHLSK IETRIDTAGL TEKEKDFLAT REKEKGNEAF NSGDYEEAVM YYTRSISALP 250 260 270 280 290 300 TVVAYNNRAQ AEIKLQNWNS AFQDCEKVLE LEPGNVKALL RRATTYKHQN KLREATEDLS 310 320 330 340 350 360 KVLDVEPDND LAKKTLSEVE RDLKNSEAAS ETQTKGKRMV IQEIENSEDE EGKSGRKHED 370 380 390 400 410 420 GGGDKKPAEP AGAARAAQPC VMGNIQKKLT GKAEGGKRPA RGAPQRGQTP EAGADKRSPR 430 440 450 460 470 480 RASAAAAAGG GATGHPGGGQ GAENPAGLKS QGNELFRSGQ FAEAAGKYSA AIALLEPAGS 490 500 510 520 530 540 EIADDLSILY SNRAACYLKE GNCSGCIQDC NRALELHPFS MKPLLRRAMA YETLEQYGKA 550 560 570 580 590 600 YVDYKTVLQI DCGLQLANDS VNRLSRILME LDGPNWREKL SPIPAVPASV PLQAWHPAKE 610 620 630 640 650 660 MISKQAGDSS SHRQQGITDE KTFKALKEEG NQCVNDKNYK DALSKYSECL KINNKECAIY 670 680 690 700 710 720 TNRALCYLKL CQFEEAKQDC DQALQLADGN VKAFYRRALA HKGLKNYQKS LIDLNKVILL 730 740 750 760 770 780 DPSIIEAKME LEEVTRLLNL KDKTAPFNKE KERRKIEIQE VNEGKEEPGR PAGEVSMGCL 790 800 810 820 830 840 ASEKGGKSSR SPEDPEKLPI AKPNNAYEFG QIINALSTRK DKEACAHLLA ITAPKDLPMF 850 860 870 880 890 900 LSNKLEGDTF LLLIQSLKNN LIEKDPSLVY QHLLYLSKAE RFKMMLTLIS KGQKELIEQL 910 920 FEDLSDTPNN HFTLEDIQAL KRQYEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression and function of the HSD-3.8 gene encoding a testis-specific protein." Lin W., Zhou X.F., Zhang M.L., Li Y., Miao S.Y., Wang L.F., Zong S.D., Koide S.S. Mol. Hum. Reprod. 7:811-818(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANT THR-777. Tissue: Testis.
[2]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-153. Tissue: Testis.
[4]	"Isolation and sequencing of the cDNA encoding the 75-kD human sperm protein related to infertility." Zhang M.L., Wang L.F., Miao S.Y., Koide S.S. Chin. Med. J. 105:998-1003(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-347 AND SER-791, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF311312 mRNA. Translation: AAG23967.1. AC025647 Genomic DNA. No translation available. BC055091 mRNA. Translation: AAH55091.1. Sequence problems.
IPI	IPI00292326.
RefSeq	NP_003105.2. NM_003114.4. NP_757367.1. NM_172218.2.
UniGene	Hs.591866.
3D structure databases
ProteinModelPortal	Q07617.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000251809.
PTM databases
PhosphoSite	Q07617.
Polymorphism databases
DMDM	223634718.
Proteomic databases
PaxDb	Q07617.
PRIDE	Q07617.
Protocols and materials databases
DNASU	6674.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000251809; ENSP00000251809; ENSG00000104450. ENST00000388798; ENSP00000373450; ENSG00000104450.
GeneID	6674.
KEGG	hsa:6674.
UCSC	uc003yjh.2. human.
Organism-specific databases
CTD	6674.
GeneCards	GC08P101170.
H-InvDB	HIX0201290.
HGNC	HGNC:11212. SPAG1.
HPA	HPA023748.
MIM	603395. gene.
neXtProt	NX_Q07617.
PharmGKB	PA36049.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0457.
HOGENOM	HOG000293399.
HOVERGEN	HBG079204.
InParanoid	Q07617.
OMA	IEAKMEL.
OrthoDB	EOG4FFD17.
Gene expression databases
ArrayExpress	Q07617.
Bgee	Q07617.
CleanEx	HS_SPAG1.
Genevestigator	Q07617.
GermOnline	ENSG00000104450. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.10. 3 hits.
InterPro	IPR025986. RPAP3-like_C. IPR001440. TPR-1. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Pfam	PF13877. RPAP3_C. 1 hit. PF00515. TPR_1. 4 hits. [Graphical view]
SMART	SM00028. TPR. 9 hits. [Graphical view]
PROSITE	PS50005. TPR. 8 hits. PS50293. TPR_REGION. 4 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6674.
NextBio	26021.
SOURCE	Search...

Entry information

Entry name

SPAG1_HUMAN

Accession

Primary (citable) accession number: Q07617
Secondary accession number(s): A6NP70, Q7Z5G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 114 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.