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Q07605

- T4BA_BACCO

UniProt

Q07605 - T4BA_BACCO

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Protein

Restriction enzyme BgcI subunit alpha

Gene

bcgIA

Organism
Bacillus coagulans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence 5'-CGAN6TGC-3' and cleaves bilaterally and symmetrically outside the sequence to release a 34-base pair fragment. Methylation of the recognition sequence occurs on the adenine in either one or both strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Cofactori

Magnesium.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. endonuclease activity Source: UniProtKB-KW
  3. N-methyltransferase activity Source: InterPro
  4. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Methyltransferase, Nuclease, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, S-adenosyl-L-methionine

Protein family/group databases

REBASEi238. BcgI.

Names & Taxonomyi

Protein namesi
Recommended name:
Restriction enzyme BgcI subunit alpha (EC:3.1.21.-)
Including the following 1 domains:
Adenine-specific methyltransferase activity (EC:2.1.1.72)
Gene namesi
Name:bcgIA
OrganismiBacillus coagulans
Taxonomic identifieri1398 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637Restriction enzyme BgcI subunit alphaPRO_0000077378Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of two alpha and one beta subunit. Both subunits are necessary for DNA-binding.

Structurei

3D structure databases

ProteinModelPortaliQ07605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07605-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNEKTSTDQ LVRRFIEDLG VTYEEQGSSN IQIKQALRSA SKSKSQSGVG
60 70 80 90 100
KPEFIFFSGN HLIIVEDKLA IDKLEYKNND GIIDTEFPFR RDYAVNGAVH
110 120 130 140 150
YARHIIEKTN SYKEVIAIGI AGDGLHYQIH PYFVSETELK KLPEMKSLED
160 170 180 190 200
ISPENIEEFY KVAVLGELPK EERELREVNK IAADMHEDLR NYGQLEGEKK
210 220 230 240 250
ATVVSAILLA LEEPTFSLNQ LIGSDRVGGT DGEIIFNAVR VYLENAGIVP
260 270 280 290 300
YAKVGEMLDQ FIFIQRDVTL NTVNSNLEMT PLKYFATTLE AEIMDKIKSN
310 320 330 340 350
TDFDILGNFY GEFVKYGGND GNPLGIVLTP RHITSLMAEL IGINKSDFVL
360 370 380 390 400
DPACGTGAFL ISAMNRMLGQ AENDDERRDI KQNRLYGIEI QQKLFTIATT
410 420 430 440 450
NMILRGDGKS NLIRDNCLTF DNTIMNGYGI NKILMNPPYS QAKNDQTQHL
460 470 480 490 500
SELSFIQQAL EMLVVGGKLC AIVPQSTMVG KNRHDKARKK QILKQHTLET
510 520 530 540 550
VITLNKDTFH GVGVNPCIVI FKAGIKHPEN KRVSFVNFED DGHVVRKHVG
560 570 580 590 600
LVGDGTEKGK REHLLAVLAG DEDDGTDLIV KTAIKDTDEW LHSFYYFNDG
610 620 630
IPSEDDFYKT VANYLTFQFD MTANGKGYLF EGVEENE
Length:637
Mass (Da):71,559
Last modified:October 1, 1994 - v1
Checksum:i0ED5ABD934E85AAF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L17341 Unassigned DNA. Translation: AAA16626.1.
PIRiA53125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L17341 Unassigned DNA. Translation: AAA16626.1 .
PIRi A53125.

3D structure databases

ProteinModelPortali Q07605.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 238. BcgI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02384. N6_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A unique restriction endonuclease, BcgI, from Bacillus coagulans."
    Kong H., Morgan R.D., Maunus R.E., Schildkraut I.
    Nucleic Acids Res. 21:987-991(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 55055 / NEB 566.
  2. "Characterization of BcgI, a new kind of restriction-modification system."
    Kong H., Roemer S.E., Waite-Rees P.A., Benner J.S., Wilson G.G., Nwankwo D.O.
    J. Biol. Chem. 269:683-690(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-14, CHARACTERIZATION.
    Strain: ATCC 55055 / NEB 566.

Entry informationi

Entry nameiT4BA_BACCO
AccessioniPrimary (citable) accession number: Q07605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Multifunctional enzyme

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3