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Q07605 (T4BA_BACCO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Restriction enzyme BgcI subunit alpha

EC=3.1.21.-

Including the following 1 domains:

  1. Adenine-specific methyltransferase activity
    EC=2.1.1.72
Gene names
Name:bcgIA
OrganismBacillus coagulans
Taxonomic identifier1398 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length637 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence 5'-CGAN6TGC-3' and cleaves bilaterally and symmetrically outside the sequence to release a 34-base pair fragment. Methylation of the recognition sequence occurs on the adenine in either one or both strands.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Cofactor

Magnesium.

Subunit structure

Heterotrimer of two alpha and one beta subunit. Both subunits are necessary for DNA-binding.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 637637Restriction enzyme BgcI subunit alpha
PRO_0000077378

Sequences

Sequence LengthMass (Da)Tools
Q07605 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 0ED5ABD934E85AAF

FASTA63771,559
        10         20         30         40         50         60 
MVNEKTSTDQ LVRRFIEDLG VTYEEQGSSN IQIKQALRSA SKSKSQSGVG KPEFIFFSGN 

        70         80         90        100        110        120 
HLIIVEDKLA IDKLEYKNND GIIDTEFPFR RDYAVNGAVH YARHIIEKTN SYKEVIAIGI 

       130        140        150        160        170        180 
AGDGLHYQIH PYFVSETELK KLPEMKSLED ISPENIEEFY KVAVLGELPK EERELREVNK 

       190        200        210        220        230        240 
IAADMHEDLR NYGQLEGEKK ATVVSAILLA LEEPTFSLNQ LIGSDRVGGT DGEIIFNAVR 

       250        260        270        280        290        300 
VYLENAGIVP YAKVGEMLDQ FIFIQRDVTL NTVNSNLEMT PLKYFATTLE AEIMDKIKSN 

       310        320        330        340        350        360 
TDFDILGNFY GEFVKYGGND GNPLGIVLTP RHITSLMAEL IGINKSDFVL DPACGTGAFL 

       370        380        390        400        410        420 
ISAMNRMLGQ AENDDERRDI KQNRLYGIEI QQKLFTIATT NMILRGDGKS NLIRDNCLTF 

       430        440        450        460        470        480 
DNTIMNGYGI NKILMNPPYS QAKNDQTQHL SELSFIQQAL EMLVVGGKLC AIVPQSTMVG 

       490        500        510        520        530        540 
KNRHDKARKK QILKQHTLET VITLNKDTFH GVGVNPCIVI FKAGIKHPEN KRVSFVNFED 

       550        560        570        580        590        600 
DGHVVRKHVG LVGDGTEKGK REHLLAVLAG DEDDGTDLIV KTAIKDTDEW LHSFYYFNDG 

       610        620        630 
IPSEDDFYKT VANYLTFQFD MTANGKGYLF EGVEENE 

« Hide

References

[1]"A unique restriction endonuclease, BcgI, from Bacillus coagulans."
Kong H., Morgan R.D., Maunus R.E., Schildkraut I.
Nucleic Acids Res. 21:987-991(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 55055 / NEB 566.
[2]"Characterization of BcgI, a new kind of restriction-modification system."
Kong H., Roemer S.E., Waite-Rees P.A., Benner J.S., Wilson G.G., Nwankwo D.O.
J. Biol. Chem. 269:683-690(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-14, CHARACTERIZATION.
Strain: ATCC 55055 / NEB 566.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L17341 Unassigned DNA. Translation: AAA16626.1.
PIRA53125.

3D structure databases

ProteinModelPortalQ07605.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE238. BcgI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
[Graphical view]
PfamPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT4BA_BACCO
AccessionPrimary (citable) accession number: Q07605
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 16, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries