Q07605 (T4BA_BACCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Restriction enzyme BgcI subunit alpha
EC=3.1.21.-

Including the following 1 domains:

1. Adenine-specific methyltransferase activity
   EC=2.1.1.72

Gene names

Name:

bcgIA

Organism

Bacillus coagulans

Taxonomic identifier

1398 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes the double-stranded sequence 5'-CGAN6TGC-3' and cleaves bilaterally and symmetrically outside the sequence to release a 34-base pair fragment. Methylation of the recognition sequence occurs on the adenine in either one or both strands.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Cofactor	Magnesium.
Subunit structure	Heterotrimer of two alpha and one beta subunit. Both subunits are necessary for DNA-binding.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Methyltransferase Nuclease Transferase
Technical term	Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	Restriction enzyme BgcI subunit alpha		PRO_0000077378

Sequences

	Sequence		Length	Mass (Da)
	Q07605 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 0ED5ABD934E85AAF	FASTA	637	71,559
	10 20 30 40 50 60 MVNEKTSTDQ LVRRFIEDLG VTYEEQGSSN IQIKQALRSA SKSKSQSGVG KPEFIFFSGN 70 80 90 100 110 120 HLIIVEDKLA IDKLEYKNND GIIDTEFPFR RDYAVNGAVH YARHIIEKTN SYKEVIAIGI 130 140 150 160 170 180 AGDGLHYQIH PYFVSETELK KLPEMKSLED ISPENIEEFY KVAVLGELPK EERELREVNK 190 200 210 220 230 240 IAADMHEDLR NYGQLEGEKK ATVVSAILLA LEEPTFSLNQ LIGSDRVGGT DGEIIFNAVR 250 260 270 280 290 300 VYLENAGIVP YAKVGEMLDQ FIFIQRDVTL NTVNSNLEMT PLKYFATTLE AEIMDKIKSN 310 320 330 340 350 360 TDFDILGNFY GEFVKYGGND GNPLGIVLTP RHITSLMAEL IGINKSDFVL DPACGTGAFL 370 380 390 400 410 420 ISAMNRMLGQ AENDDERRDI KQNRLYGIEI QQKLFTIATT NMILRGDGKS NLIRDNCLTF 430 440 450 460 470 480 DNTIMNGYGI NKILMNPPYS QAKNDQTQHL SELSFIQQAL EMLVVGGKLC AIVPQSTMVG 490 500 510 520 530 540 KNRHDKARKK QILKQHTLET VITLNKDTFH GVGVNPCIVI FKAGIKHPEN KRVSFVNFED 550 560 570 580 590 600 DGHVVRKHVG LVGDGTEKGK REHLLAVLAG DEDDGTDLIV KTAIKDTDEW LHSFYYFNDG 610 620 630 IPSEDDFYKT VANYLTFQFD MTANGKGYLF EGVEENE

References

[1]	"A unique restriction endonuclease, BcgI, from Bacillus coagulans." Kong H., Morgan R.D., Maunus R.E., Schildkraut I. Nucleic Acids Res. 21:987-991(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 55055 / NEB 566.
[2]	"Characterization of BcgI, a new kind of restriction-modification system." Kong H., Roemer S.E., Waite-Rees P.A., Benner J.S., Wilson G.G., Nwankwo D.O. J. Biol. Chem. 269:683-690(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-14, CHARACTERIZATION. Strain: ATCC 55055 / NEB 566.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L17341 Unassigned DNA. Translation: AAA16626.1.
PIR	A53125.
3D structure databases
ProteinModelPortal	Q07605.
ModBase	Search...
Protein family/group databases
REBASE	238. BcgI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
Pfam	PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T4BA_BACCO

Accession

Primary (citable) accession number: Q07605

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program