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Protein

Restriction enzyme BgcI subunit alpha

Gene

bcgIA

Organism
Bacillus coagulans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence 5'-CGAN6TGC-3' and cleaves bilaterally and symmetrically outside the sequence to release a 34-base pair fragment. Methylation of the recognition sequence occurs on the adenine in either one or both strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Methyltransferase, Nuclease, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, S-adenosyl-L-methionine

Protein family/group databases

REBASEi238. BcgI.

Names & Taxonomyi

Protein namesi
Recommended name:
Restriction enzyme BgcI subunit alpha (EC:3.1.21.-)
Including the following 1 domains:
Adenine-specific methyltransferase activity (EC:2.1.1.72)
Gene namesi
Name:bcgIA
OrganismiBacillus coagulans
Taxonomic identifieri1398 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637Restriction enzyme BgcI subunit alphaPRO_0000077378Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of two alpha and one beta subunit. Both subunits are necessary for DNA-binding.

Structurei

3D structure databases

ProteinModelPortaliQ07605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNEKTSTDQ LVRRFIEDLG VTYEEQGSSN IQIKQALRSA SKSKSQSGVG
60 70 80 90 100
KPEFIFFSGN HLIIVEDKLA IDKLEYKNND GIIDTEFPFR RDYAVNGAVH
110 120 130 140 150
YARHIIEKTN SYKEVIAIGI AGDGLHYQIH PYFVSETELK KLPEMKSLED
160 170 180 190 200
ISPENIEEFY KVAVLGELPK EERELREVNK IAADMHEDLR NYGQLEGEKK
210 220 230 240 250
ATVVSAILLA LEEPTFSLNQ LIGSDRVGGT DGEIIFNAVR VYLENAGIVP
260 270 280 290 300
YAKVGEMLDQ FIFIQRDVTL NTVNSNLEMT PLKYFATTLE AEIMDKIKSN
310 320 330 340 350
TDFDILGNFY GEFVKYGGND GNPLGIVLTP RHITSLMAEL IGINKSDFVL
360 370 380 390 400
DPACGTGAFL ISAMNRMLGQ AENDDERRDI KQNRLYGIEI QQKLFTIATT
410 420 430 440 450
NMILRGDGKS NLIRDNCLTF DNTIMNGYGI NKILMNPPYS QAKNDQTQHL
460 470 480 490 500
SELSFIQQAL EMLVVGGKLC AIVPQSTMVG KNRHDKARKK QILKQHTLET
510 520 530 540 550
VITLNKDTFH GVGVNPCIVI FKAGIKHPEN KRVSFVNFED DGHVVRKHVG
560 570 580 590 600
LVGDGTEKGK REHLLAVLAG DEDDGTDLIV KTAIKDTDEW LHSFYYFNDG
610 620 630
IPSEDDFYKT VANYLTFQFD MTANGKGYLF EGVEENE
Length:637
Mass (Da):71,559
Last modified:October 1, 1994 - v1
Checksum:i0ED5ABD934E85AAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17341 Unassigned DNA. Translation: AAA16626.1.
PIRiA53125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17341 Unassigned DNA. Translation: AAA16626.1.
PIRiA53125.

3D structure databases

ProteinModelPortaliQ07605.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi238. BcgI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A unique restriction endonuclease, BcgI, from Bacillus coagulans."
    Kong H., Morgan R.D., Maunus R.E., Schildkraut I.
    Nucleic Acids Res. 21:987-991(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 55055 / NEB 566.
  2. "Characterization of BcgI, a new kind of restriction-modification system."
    Kong H., Roemer S.E., Waite-Rees P.A., Benner J.S., Wilson G.G., Nwankwo D.O.
    J. Biol. Chem. 269:683-690(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-14, CHARACTERIZATION.
    Strain: ATCC 55055 / NEB 566.

Entry informationi

Entry nameiT4BA_BACCO
AccessioniPrimary (citable) accession number: Q07605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 27, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Multifunctional enzyme

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.