Q07563 (COHA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Collagen alpha-1(XVII) chain

Alternative name(s):
180 kDa bullous pemphigoid antigen 2
Bullous pemphigoid antigen 2

Cleaved into the following chain:

1. 120 kDa linear IgA disease antigen homolog

Gene names

Name:	Col17a1
Synonyms:	Bp180, Bpag2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane By similarity. The 120 kDa linear IgA disease antigen homolog is an anchoring filament component involved in dermal-epidermal cohesion By similarity.
Subunit structure	Homotrimers of alpha 1(XVII)chains. Interacts (via cytoplasmic region) with ITGB4 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST (via N-terminus). Interacts (via N-terminus) with PLEC. Interacts (via cytoplasmic region) with DSP By similarity.
Subcellular location	Cell junction › hemidesmosome. Membrane; Single-pass type II membrane protein. Note: Localized along the plasma membrane of the hemidesmosome By similarity. 120 kDa linear IgA disease antigen homolog: Secreted › extracellular space › extracellular matrix › basement membrane By similarity.
Post-translational modification	The intracellular/endo domain is disulfide-linked By similarity. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen homolog. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosophorylation at Ser-551 By similarity.

Ontologies

Keywords
Cellular component	Basement membrane Cell junction Extracellular matrix Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Collagen Repeat Signal-anchor Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Hydroxylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hemidesmosome assembly Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	basement membrane Inferred from electronic annotation. Source: UniProtKB-SubCell collagen Inferred from electronic annotation. Source: UniProtKB-KW hemidesmosome Inferred from direct assay PubMed 8707838. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ELANE	P08246	2	EBI-6251005,EBI-986345	From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q07563-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q07563-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1157-1194: GSDYRNIIGPPGPPGPPGMPGNAWSSISVEDLSSYLHT → A

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1470	1470	Collagen alpha-1(XVII) chain		PRO_0000059408
	Chain	531 – 1470	940	120 kDa linear IgA disease antigen homolog By similarity		PRO_0000342558
Regions
	Topological domain	1 – 476	476	Cytoplasmic Potential
	Transmembrane	477 – 497	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	498 – 1470	973	Extracellular Potential
	Region	1 – 573	573	Nonhelical region (NC16)
	Region	146 – 231	86	Necessary for interaction with DST and for the recruitment of DST to hemidesmosome By similarity
	Region	574 – 1456	883	Triple-helical region
	Region	1457 – 1470	14	Nonhelical region (NC1)
Amino acid modifications
	Modified residue	85	1	Phosphoserine Ref.5
	Modified residue	93	1	Phosphoserine Ref.5
	Modified residue	149	1	Phosphoserine Ref.5
	Modified residue	551	1	Phosphoserine; by CK2 By similarity
	Glycosylation	1273	1	N-linked (GlcNAc...) Potential
	Glycosylation	1395	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1157 – 1194	38	GSDYR…SYLHT → A in isoform 2.		VSP_009362
Experimental info
	Sequence conflict	164	1	R → S in AAA37443. Ref.1
	Sequence conflict	289	1	A → G in AAA37443. Ref.1
	Sequence conflict	324	1	S → C in AAA37443. Ref.1
	Sequence conflict	1275	1	S → G in AAH03208. Ref.3
	Sequence conflict	1277	1	N → S in AAA37443. Ref.1
	Sequence conflict	1277	1	N → S in AAH03208. Ref.3
	Sequence conflict	1277	1	N → S in AAI25032. Ref.3
	Sequence conflict	1277	1	N → S in AAI25033. Ref.3
	Sequence conflict	1292	1	T → I in AAA37443. Ref.1
	Sequence conflict	1292	1	T → I in AAH03208. Ref.3
	Sequence conflict	1292	1	T → I in AAI25032. Ref.3
	Sequence conflict	1292	1	T → I in AAI25033. Ref.3
	Sequence conflict	1388	1	R → W in AAA37443. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2007. Version 3. Checksum: 7C38356D6ED68347	FASTA	1,470	147,975
	10 20 30 40 50 60 MDVTKKSKRD GTEVTERIVT ETVTTRLTSL PPKGSTSNGY AKTGSLGGGS RLEKQSLTHG 70 80 90 100 110 120 SSGYINSSGS IRGNASTSSY RRTHSPASTL PNSPGSTFER KAHMTRHGTY EGSSSGNSSP 130 140 150 160 170 180 EYPRKELASS STRGRSQTRE SEIRVRLQSA SPSTRWTELD EVKRLLKGSR SASASPTRNT 190 200 210 220 230 240 SNTLPIPKKG TVETKTVTAS SHSVSGTYDS AILDTNFPPH MWSSTLPAGS SLGTYQNNIT 250 260 270 280 290 300 AQSTSLLNTN AYSTGSVFGV PNNMASCSPT LHPGLSSCSS VFGMQNNLAP SSSVLSHGTT 310 320 330 340 350 360 TASTAYGAKK NVPQPPTVTS TGVSTSATCT TSVQSDDLLH KDCKFLILEK DNTPAKKEME 370 380 390 400 410 420 LLIMTKDSGK VFTASPATIS STSFSEDTLK KEKQAAYAAD TCLKADVNGD LNTVSTKSKM 430 440 450 460 470 480 TSAENHGYDR GGGGGRGKGG GAGGGGGGGG ASGGGGAWGA APAWCPCGSC CSWWKWLLGL 490 500 510 520 530 540 LLTWLLLLGL LFGLIALAEE VRKLKARVEE LEKTKVLYHD VQMDKSNRDR LQAEAPSLGP 550 560 570 580 590 600 GLGKAELDGY SQEAIWLFVR NKLMTEQENG NLRGSPGPKG DMGSQGPKGD RGLPGTPGIP 610 620 630 640 650 660 GPLGHPGPEG PKGQKGSIGD PGMEGPIGQR GLAGPMGPRG EPGPPGSGEK GDRGIAGEQG 670 680 690 700 710 720 PQGLPGVPGP PGLRGHSGSP GPQGPPGAVG PQGLRGDVGL PGVKGDKGLM GPPGPKGDQG 730 740 750 760 770 780 EKGPRGLTGE PGIRGLPGAV GEPGAKGAMG PAGADGQQGS RGEQGLTGMP GTRGPPGPAG 790 800 810 820 830 840 DPGKPGLTGP QGPQGLPGSP GRPGTKGEPG APGRVMTSEG SSTITVPGPP GPPGAMGPPG 850 860 870 880 890 900 PPGTPGPAGP AGLPGQQGPR GEPGLAGDSF LSSGSSISEV LSAQGVDLRG PPGPPGPRGP 910 920 930 940 950 960 PGPSIPGPPG PRGPPGEGVP GPPGPPGSFL TDSETFFTGP PGPPGPPGPK GDQGDPGVPG 970 980 990 1000 1010 1020 TPGISGGLSH GASSSTLYMQ GPPGPPGPPG PPGSLSSSGQ DIQHYIAEYM QSDNIRTYLS 1030 1040 1050 1060 1070 1080 GVQGPPGPPG PPGPVITITG ETFDYSQLAS QVVSYLRSSG YGAGLSSASS SEDILAMLRR 1090 1100 1110 1120 1130 1140 NDVWQYLRQN LVGPPGPPGP PGVSGDGSLL SLDYGELSRH ILNYMSSSGI SFGHPGPPGP 1150 1160 1170 1180 1190 1200 PGLPGTSYEE LLTMLRGSDY RNIIGPPGPP GPPGMPGNAW SSISVEDLSS YLHTAGLSSI 1210 1220 1230 1240 1250 1260 PGPPGPPGPP GPRGPPGVSA ALSTYAAENS DNFRSELISY LTSPDVRSFI VGPPGPPGPQ 1270 1280 1290 1300 1310 1320 GPPGDGHLRE NYNWSSNSSA RRGTSYSSST GTGGTNGGSL GEGGAYGAGD GGPYGTDIGP 1330 1340 1350 1360 1370 1380 GGGYGAAAGG GIYGTNGDSF RDGFTGDLDY NKLAVRVSES MQRQGLLQGM AYTVQGPPGP 1390 1400 1410 1420 1430 1440 QGPPGISRVF SAYSNVTQDL MDFFQTYGTI PGPPGQKGDV GTPGPKGDRG PAGPRGPPGP 1450 1460 1470 PGPRGNKGEK GDKGDQVYTG RRKRSIAIKP
	Isoform 2 [UniParc]. Checksum: 0C8997CF58634BA1	FASTA	1,433	144,140
	10 20 30 40 50 60 MDVTKKSKRD GTEVTERIVT ETVTTRLTSL PPKGSTSNGY AKTGSLGGGS RLEKQSLTHG 70 80 90 100 110 120 SSGYINSSGS IRGNASTSSY RRTHSPASTL PNSPGSTFER KAHMTRHGTY EGSSSGNSSP 130 140 150 160 170 180 EYPRKELASS STRGRSQTRE SEIRVRLQSA SPSTRWTELD EVKRLLKGSR SASASPTRNT 190 200 210 220 230 240 SNTLPIPKKG TVETKTVTAS SHSVSGTYDS AILDTNFPPH MWSSTLPAGS SLGTYQNNIT 250 260 270 280 290 300 AQSTSLLNTN AYSTGSVFGV PNNMASCSPT LHPGLSSCSS VFGMQNNLAP SSSVLSHGTT 310 320 330 340 350 360 TASTAYGAKK NVPQPPTVTS TGVSTSATCT TSVQSDDLLH KDCKFLILEK DNTPAKKEME 370 380 390 400 410 420 LLIMTKDSGK VFTASPATIS STSFSEDTLK KEKQAAYAAD TCLKADVNGD LNTVSTKSKM 430 440 450 460 470 480 TSAENHGYDR GGGGGRGKGG GAGGGGGGGG ASGGGGAWGA APAWCPCGSC CSWWKWLLGL 490 500 510 520 530 540 LLTWLLLLGL LFGLIALAEE VRKLKARVEE LEKTKVLYHD VQMDKSNRDR LQAEAPSLGP 550 560 570 580 590 600 GLGKAELDGY SQEAIWLFVR NKLMTEQENG NLRGSPGPKG DMGSQGPKGD RGLPGTPGIP 610 620 630 640 650 660 GPLGHPGPEG PKGQKGSIGD PGMEGPIGQR GLAGPMGPRG EPGPPGSGEK GDRGIAGEQG 670 680 690 700 710 720 PQGLPGVPGP PGLRGHSGSP GPQGPPGAVG PQGLRGDVGL PGVKGDKGLM GPPGPKGDQG 730 740 750 760 770 780 EKGPRGLTGE PGIRGLPGAV GEPGAKGAMG PAGADGQQGS RGEQGLTGMP GTRGPPGPAG 790 800 810 820 830 840 DPGKPGLTGP QGPQGLPGSP GRPGTKGEPG APGRVMTSEG SSTITVPGPP GPPGAMGPPG 850 860 870 880 890 900 PPGTPGPAGP AGLPGQQGPR GEPGLAGDSF LSSGSSISEV LSAQGVDLRG PPGPPGPRGP 910 920 930 940 950 960 PGPSIPGPPG PRGPPGEGVP GPPGPPGSFL TDSETFFTGP PGPPGPPGPK GDQGDPGVPG 970 980 990 1000 1010 1020 TPGISGGLSH GASSSTLYMQ GPPGPPGPPG PPGSLSSSGQ DIQHYIAEYM QSDNIRTYLS 1030 1040 1050 1060 1070 1080 GVQGPPGPPG PPGPVITITG ETFDYSQLAS QVVSYLRSSG YGAGLSSASS SEDILAMLRR 1090 1100 1110 1120 1130 1140 NDVWQYLRQN LVGPPGPPGP PGVSGDGSLL SLDYGELSRH ILNYMSSSGI SFGHPGPPGP 1150 1160 1170 1180 1190 1200 PGLPGTSYEE LLTMLRAAGL SSIPGPPGPP GPPGPRGPPG VSAALSTYAA ENSDNFRSEL 1210 1220 1230 1240 1250 1260 ISYLTSPDVR SFIVGPPGPP GPQGPPGDGH LRENYNWSSN SSARRGTSYS SSTGTGGTNG 1270 1280 1290 1300 1310 1320 GSLGEGGAYG AGDGGPYGTD IGPGGGYGAA AGGGIYGTNG DSFRDGFTGD LDYNKLAVRV 1330 1340 1350 1360 1370 1380 SESMQRQGLL QGMAYTVQGP PGPQGPPGIS RVFSAYSNVT QDLMDFFQTY GTIPGPPGQK 1390 1400 1410 1420 1430 GDVGTPGPKG DRGPAGPRGP PGPPGPRGNK GEKGDKGDQV YTGRRKRSIA IKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of type XVII collagen: complementary and genomic DNA sequences of mouse 180-kDa bullous pemphigoid antigen (BPAG2) predict an interrupted collagenous domain, a transmembrane segment, and unusual features in the 5'-end of the gene and the 3' -." Li K., Tamai K., Tan E.M.L., Uitto J. J. Biol. Chem. 268:8825-8834(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: BALB/c.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary gland.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1357-1470. Strain: C57BL/6J. Tissue: Cerebellum.
[5]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85; SER-93 AND SER-149, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08407 mRNA. Translation: AAA37443.1. AC131719 Genomic DNA. No translation available. BC003208 mRNA. Translation: AAH03208.1. BC125031 mRNA. Translation: AAI25032.1. BC125032 mRNA. Translation: AAI25033.1. AK135150 mRNA. Translation: BAE22442.1.
IPI	IPI00284644. IPI00399843.
PIR	A46053.
RefSeq	NP_031758.2. NM_007732.2.
UniGene	Mm.1225.
3D structure databases
ProteinModelPortal	Q07563.
SMR	Q07563. Positions 1404-1440.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q07563. 1 interaction.
PTM databases
PhosphoSite	Q07563.
Proteomic databases
PaxDb	Q07563.
PRIDE	Q07563.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026045; ENSMUSP00000026045; ENSMUSG00000025064. ENSMUST00000086923; ENSMUSP00000084141; ENSMUSG00000025064.
GeneID	12821.
KEGG	mmu:12821.
UCSC	uc008hvi.2. mouse. uc008hvj.2. mouse.
Organism-specific databases
CTD	1308.
MGI	MGI:88450. Col17a1.
Phylogenomic databases
eggNOG	NOG309396.
GeneTree	ENSGT00700000104250.
HOGENOM	HOG000111885.
HOVERGEN	HBG051065.
InParanoid	Q07563.
KO	K07603.
OMA	LDYAELS.
OrthoDB	EOG4RNB7V.
Gene expression databases
Bgee	Q07563.
CleanEx	MM_COL17A1.
Genevestigator	Q07563.
GermOnline	ENSMUSG00000025064. Mus musculus.
Family and domain databases
InterPro	IPR008160. Collagen. [Graphical view]
Pfam	PF01391. Collagen. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	282294.
SOURCE	Search...

Entry information

Entry name

COHA1_MOUSE

Accession

Primary (citable) accession number: Q07563
Secondary accession number(s): Q08AT3, Q3UXX1, Q99LK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	May 1, 2007
Last modified:	April 3, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program