Reviewed, UniProtKB/Swiss-Prot Q07551 (KAR_YEAST)

Names and origin

Protein names

Recommended name:
    NADPH-dependent alpha-keto amide reductase
      Short name=AKR-E
    EC=1.2.1.-
Alternative name(s):
    YKAR

Gene names

Ordered Locus Names:	YDL124W
ORF Names:	D2240

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces aromatic alpha-keto amides, aliphatic and aromatic alpha-keto esters, but not beta-keto esters. Ref.3
Subunit structure	Monomer. Ref.2
Subcellular location	Cytoplasm. Nucleus. Ref.4
Induction	Transiently induced shortly after the switch from aerobic to anaerobic growth (at protein level). Ref.6
Post-translational modification	The N-terminus is blocked.
Miscellaneous	Present with 4030 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the aldo/keto reductase family.
Biophysicochemical properties	Kinetic parameters: KM=146 µM for o-chlorobenzoylformamide KM=156 µM for m-chlorobenzoylformamide KM=231 µM for p-chlorobenzoylformamide KM=103 µM for benzoylformamide KM=254 µM for 3-methyl-2-oxobutanoate pH dependence: Stable from pH 6 to 9.5. Temperature dependence: Thermostable up to 40 degrees Celsius.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular amide metabolic process Ref.2 Inferred from direct assay. Source: SGD cellular aromatic compound metabolic process Inferred from direct assay. Source: SGD cellular ketone metabolic process Inferred from direct assay. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD nucleus Ref.4 Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	aldehyde reductase activity Ref.3 Inferred from direct assay. Source: SGD alpha-keto amide reductase activity Ref.2 Inferred from direct assay. Source: SGD alpha-keto ester reductase activity Ref.2 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	NADPH-dependent alpha-keto amide reductase		PRO_0000262755
Regions
	Nucleotide binding	208 – 268	61	NADP By similarity
Sites
	Active site	64	1	Proton donor By similarity
	Binding site	122	1	Substrate By similarity
	Site	89	1	Lowers pKa of active site Tyr By similarity
Amino acid modifications
	Modified residue	123	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q07551-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 165ADCB5C1952AF0	FASTA	312	35,561
	10 20 30 40 50 60 MSFHQQFFTL NNGNKIPAIA IIGTGTRWYK NEETDATFSN SLVEQIVYAL KLPGIIHIDA 70 80 90 100 110 120 AEIYRTYPEV GKALSLTEKP RNAIFLTDKY SPQIKMSDSP ADGLDLALKK MGTDYVDLYL 130 140 150 160 170 180 LHSPFVSKEV NGLSLEEAWK DMEQLYKSGK AKNIGVSNFA VEDLQRILKV AEVKPQVNQI 190 200 210 220 230 240 EFSPFLQNQT PGIYKFCQEH DILVEAYSPL GPLQKKTAQD DSQPFFEYVK ELSEKYIKSE 250 260 270 280 290 300 AQIILRWVTK RGVLPVTTSS KPQRISDAQN LFSFDLTAEE VDKITELGLE HEPLRLYWNK 310 LYGKYNYAAQ KV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Purification and characterization of alpha-keto amide reductase from Saccharomyces cerevisiae." Ishihara K., Yamamoto H., Mitsuhashi K., Nishikawa K., Tsuboi S., Tsuji H., Nakajima N. Biosci. Biotechnol. Biochem. 68:2306-2312(2004) [PubMed: 15564669] [Abstract] Cited for: PROTEIN SEQUENCE OF 265-276, BIOPHYSICOCHEMICAL PROPERTIES, CHARACTERIZATION, SUBUNIT.
[3]	"Functional genomic studies of aldo-keto reductases." Petrash J.M., Murthy B.S., Young M., Morris K., Rikimaru L., Griest T.A., Harter T. Chem. Biol. Interact. 130:673-683(2001) [PubMed: 11306085] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome analysis of recombinant xylose-fermenting Saccharomyces cerevisiae." Salusjaervi L., Poutanen M., Pitkaenen J.-P., Koivistoinen H., Aristidou A., Kalkkinen N., Ruohonen L., Penttilae M. Yeast 20:295-314(2003) [PubMed: 12627397] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z74172 Genomic DNA. Translation: CAA98692.1.
PIR	S67667.
RefSeq	NP_010159.1.
3D structure databases
HSSP	HSSP built from PDB template 1K8C based on UniProtKB O74237.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6607N.
Proteomic databases
PeptideAtlas	Q07551.
Genome annotation databases
Ensembl	YDL124W. Saccharomyces cerevisiae. [Contig view]
GeneID	851433.
GenomeReviews	Gene locus YDL124W in contig Z71256_GR.
KEGG	sce:YDL124W.
NMPDR	fig|4932.3.peg.896.
Organism-specific databases
CYGD	YDL124w.
SGD	S000002282. YDL124W.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q07551.
OMA	Q07551. NTIPAVG.
Gene expression databases
ArrayExpress	Q07551.
GermOnline	YDL124W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. False negative. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968660.

Entry information

Entry name

KAR_YEAST

Accession

Primary (citable) accession number: Q07551

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)