Reviewed, UniProtKB/Swiss-Prot Q07540 (FRDA_YEAST)

Names and origin

Protein names

Recommended name:
    Frataxin homolog, mitochondrial
Cleaved into the following chain:
    1- Recommended name:
            Frataxin homolog intermediate form

Gene names

Name:	YFH1
Ordered Locus Names:	YDL120W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to be involved in the regulation of mitochondrial iron efflux. Ref.3 Ref.4
Subunit structure	Interacts with ISU1. Ref.7 Ref.8
Subcellular location	Mitochondrion matrix Ref.3 Ref.4.
Post-translational modification	Processed in two steps by mitochondrial processing peptidase (MPP). MPP first cleaves the precursor to intermediate form and subsequently converts the intermediate to mature size protein.
Miscellaneous	Present with 1560 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the frataxin family.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Iron
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cellular iron ion homeostasis Ref.3 Inferred from direct assay. Source: SGD glutathione metabolic process Inferred from mutant phenotype. Source: SGD iron ion transport Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster assembly Ref.8 Inferred from direct assay. Source: SGD
Cellular component	mitochondrial matrix Ref.6 Inferred from direct assay. Source: SGD
Molecular function	ferrous iron binding Inferred from direct assay. Source: SGD ferroxidase activity Inferred from direct assay. Source: SGD iron chaperone activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 21

21

Mitochondrion Probable

Chain

22 – 174

153

Frataxin homolog intermediate form

PRO_0000010135

Chain

52 – 174

123

Frataxin homolog, mitochondrial

PRO_0000010136

Secondary structure

1

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174

Helix Strand Turn

Details...

Turn

63 – 67

5

Helix

76 – 89

14

Beta strand

92 – 94

3

Beta strand

96 – 99

4

Beta strand

101 – 103

3

Beta strand

106 – 113

8

Turn

114 – 116

3

Beta strand

117 – 123

7

Beta strand

126 – 134

9

Turn

135 – 137

3

Beta strand

138 – 147

10

Beta strand

149 – 151

3

Turn

152 – 154

3

Helix

158 – 171

14

Sequences

	Sequence		Length	Mass (Da)
	Q07540-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AB1FF7478EF0E5D6	FASTA	174	19,490
	10 20 30 40 50 60 MIKRSLASLV RVSSVMGRRY MIAAAGGERA RFCPAVTNKK NHTVNTFQKR FVESSTDGQV 70 80 90 100 110 120 VPQEVLNLPL EKYHEEADDY LDHLLDSLEE LSEAHPDCIP DVELSHGVMT LEIPAFGTYV 130 140 150 160 170 INKQPPNKQI WLASPLSGPN RFDLLNGEWV SLRNGTKLTD ILTEEVEKAI SKSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Regulation of mitochondrial iron accumulation by Yfh1p, a putative homolog of frataxin." Babcock M., de Silva D., Oaks R., Davis-Kaplan S., Jiralerspong S., Montermini L., Pandolfo M., Kaplan J. Science 276:1709-1712(1997) [PubMed: 9180083] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"The yeast frataxin homologue mediates mitochondrial iron efflux. Evidence for a mitochondrial iron cycle." Radisky D.C., Babcock M.C., Kaplan J. J. Biol. Chem. 274:4497-4499(1999) [PubMed: 9988680] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Maturation of frataxin within mammalian and yeast mitochondria: one-step processing by matrix processing peptidase." Gordon D.M., Shi Q., Dancis A., Pain D. Hum. Mol. Genet. 8:2255-2262(1999) [PubMed: 10545606] [Abstract] Cited for: PROCESSING.
[6]	"Yeast and human frataxin are processed to mature form in two sequential steps by the mitochondrial processing peptidase." Branda S.S., Cavadini P., Adamec J., Kalousek F., Taroni F., Isaya G. J. Biol. Chem. 274:22763-22769(1999) [PubMed: 10428860] [Abstract] Cited for: PROCESSING.
[7]	"Saccharomyces cerevisiae ISU1 and ISU2: members of a well-conserved gene family for iron-sulfur cluster assembly." Garland S.A., Hoff K., Vickery L.E., Culotta V.C. J. Mol. Biol. 294:897-907(1999) [PubMed: 10588895] [Abstract] Cited for: INTERACTION WITH ISU1.
[8]	"An interaction between frataxin and Isu1/Nfs1 that is crucial for Fe/S cluster synthesis on Isu1." Gerber J., Muhlenhoff U., Lill R. EMBO Rep. 4:906-911(2003) [PubMed: 12947415] [Abstract] Cited for: INTERACTION WITH ISU1.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z74168 Genomic DNA. Translation: CAA98688.1.
AY558160 Genomic DNA. Translation: AAS56486.1.

PIR

S67663.

RefSeq

NP_010163.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FQL	X-ray	3.01	A	52-174	[»]
2GA5	NMR	-	A	53-174	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-7485N.

IntAct

Q07540. 6 interactions.

STRING

Q07540.

Genome annotation databases

Ensembl

YDL120W; YDL120W; YDL120W; Saccharomyces cerevisiae. [Genome view]

GeneID

851437.

KEGG

sce:YDL120W.

NMPDR

fig|4932.3.peg.900.

Organism-specific databases

CYGD

YDL120w.

SGD

S000002278. YFH1.

Phylogenomic databases

eggNOG

fuNOG11011.

HOGENOM

HBG396998.

OMA

HIPEVEY.

OrthoDB

EOG98963R.

PhylomeDB

Q07540.

Gene expression databases

ArrayExpress

Q07540.

Genevestigator

Q07540.

GermOnline

YDL120W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR017789. Frataxin.
IPR002908. Frataxin-like.
IPR020895. Frataxin_CS.
[Graphical view]

Gene3D

G3DSA:3.30.920.10. Frataxin_like. 1 hit.

Pfam

PF01491. Frataxin_Cyay. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03421. FeS_CyaY. 1 hit.
TIGR03422. mito_frataxin. 1 hit.

PROSITE

PS01344. FRATAXIN_1. 1 hit.
PS50810. FRATAXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

968672.

Entry information

Entry name

FRDA_YEAST

Accession

Primary (citable) accession number: Q07540

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)