ID   MMSA_BOVIN              Reviewed;         537 AA.
AC   Q07536;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   16-JUN-2009, entry version 72.
DE   RecName: Full=Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial;
DE            Short=MMSDH;
DE            Short=Malonate-semialdehyde dehydrogenase [acylating];
DE            EC=1.2.1.27;
DE            EC=1.2.1.18;
DE   AltName: Full=Aldehyde dehydrogenase family 6 member A1;
DE   Flags: Precursor;
GN   Name=ALDH6A1; Synonyms=MMSDH;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Liver;
RX   MEDLINE=93293905; PubMed=8514806;
RA   Deichaite I., Berthiaume L., Peseckis S.M., Patton W.F., Resh M.D.;
RT   "Novel use of an iodo-myristyl-CoA analog identifies a semialdehyde
RT   dehydrogenase in bovine liver.";
RL   J. Biol. Chem. 268:13738-13747(1993).
CC   -!- FUNCTION: Plays a role in valine and pyrimidine metabolism. Binds
CC       fatty acyl-CoA.
CC   -!- CATALYTIC ACTIVITY: 2-methyl-3-oxopropanoate + CoA + H(2)O +
CC       NAD(+) = propanoyl-CoA + HCO(3)(-) + NADH.
CC   -!- CATALYTIC ACTIVITY: 3-oxopropanoate + CoA + NAD(P)(+) = acetyl-CoA
CC       + CO(2) + NAD(P)H.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; L08643; AAA30650.1; -; mRNA.
DR   IPI; IPI00698279; -.
DR   PIR; A46600; A46600.
DR   RefSeq; NP_787005.1; -.
DR   UniGene; Bt.98765; -.
DR   HSSP; Q28399; 1O9J.
DR   Ensembl; ENSBTAG00000018469; Bos taurus.
DR   GeneID; 327692; -.
DR   KEGG; bta:327692; -.
DR   HOVERGEN; Q07536; -.
DR   BRENDA; 1.2.1.18; 251.
DR   BRENDA; 1.2.1.27; 251.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0000062; F:acyl-CoA binding; IDA:UniProtKB.
DR   GO; GO:0018478; F:malonate-semialdehyde dehydrogenase (acetyl...; ISS:UniProtKB.
DR   GO; GO:0004491; F:methylmalonate-semialdehyde dehydrogenase (...; IDA:UniProtKB.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0019859; P:thymine metabolic process; ISS:UniProtKB.
DR   GO; GO:0006573; P:valine metabolic process; ISS:UniProtKB.
DR   InterPro; IPR016160; Ald_DH_CS.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH.
DR   InterPro; IPR010061; MeMal-semiAld_DH.
DR   Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
DR   PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR   PANTHER; PTHR11699:SF27; MMSDH; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   TIGRFAMs; TIGR01722; MMSDH; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Acetylation; Direct protein sequencing; Mitochondrion; NAD;
KW   Oxidoreductase; Transit peptide.
FT   TRANSIT       1     34       Mitochondrion (By similarity).
FT   CHAIN        35    537       Methylmalonate-semialdehyde dehydrogenase
FT                                [acylating], mitochondrial.
FT                                /FTId=PRO_0000007188.
FT   NP_BIND     211    215       NAD (Potential).
FT   NP_BIND     263    268       NAD (Potential).
FT   ACT_SITE    319    319       Nucleophile (By similarity).
FT   BINDING     419    419       NAD (Potential).
FT   MOD_RES      57     57       N6-acetyllysine (By similarity).
FT   MOD_RES     119    119       N6-acetyllysine (By similarity).
FT   MOD_RES     333    333       N6-acetyllysine (By similarity).
FT   CONFLICT    126    127       ML -> TD (in Ref. 1; AA sequence).
SQ   SEQUENCE   537 AA;  58063 MW;  69DF39506E62F9C0 CRC64;
     MAAVAVAAAA AALRARILQV SSKVNSSWQP ASSFSSSSVP TVKLFIDGKF IESKSDKWID
     IHNPATNEVI GRVPESTKAE MDAAVSSCKR TFPAWADTSI LSRQQVLLRY QQLIKENLKE
     IARLIMLEQG KTLADAEGDV FRGLQVVEHA CSVTSLMLGD TMPSITKDMD LYSYRLPLGV
     CAGIAPFNFP AMIPLWMFPM AMVCGNTFLM KPSERVPGAT MLLAKLFQDS GAPDGTLNII
     HGQHEAVNFI CDHPDIKAIS FVGSNQAGEY IFERGSRHGK RVQANMGAKN HGVVMPDANK
     ENTLNQLVGA AFGAAGQRCM ALSTAILVGE AKKWLPELVE RAKKLRVNAG DQPGADLGPL
     ITPQAKERVC NLIDSGTKEG ASILLDGRSI KVKGYENGNF VGPTIISNVK PNMTCYKEEI
     FGPVLVVLET DTLDEAIKIV NDNPYGNGTA IFTTNGATAR KYSHLVDVGQ VGVNVPIPVP
     LPMFSFTGSR ASFRGDTNFY GKQGIQFYTQ LKTITSQWKE EDASLSSPAV VMPTMGR
//