Reviewed, UniProtKB/Swiss-Prot Q07536 (MMSA_BOVIN)

Names and origin

Protein names

Recommended name:
    Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
      Short name=MMSDH
      Short name=Malonate-semialdehyde dehydrogenase [acylating]
    EC=1.2.1.27
    EC=1.2.1.18
Alternative name(s):
    Aldehyde dehydrogenase family 6 member A1

Gene names

Name:	ALDH6A1
Synonyms:	MMSDH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Catalytic activity	2-methyl-3-oxopropanoate + CoA + H(2)O + NAD(+) = propanoyl-CoA + HCO(3)(-) + NADH. 3-oxopropanoate + CoA + NAD(P)(+) = acetyl-CoA + CO(2) + NAD(P)H.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW thymine metabolic process Inferred from sequence or structural similarity. Source: UniProtKB valine metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyl-CoA binding Ref.1 Inferred from direct assay. Source: UniProtKB malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from sequence or structural similarity. Source: UniProtKB methylmalonate-semialdehyde dehydrogenase (acylating) activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion By similarity
	Chain	35 – 537	503	Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial		PRO_0000007188
Regions
	Nucleotide binding	211 – 215	5	NAD Potential
	Nucleotide binding	263 – 268	6	NAD Potential
Sites
	Active site	319	1	Nucleophile By similarity
	Binding site	419	1	NAD Potential
Amino acid modifications
	Modified residue	57	1	N6-acetyllysine By similarity
	Modified residue	119	1	N6-acetyllysine By similarity
	Modified residue	333	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	126 – 127	2	ML → TD AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q07536-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 69DF39506E62F9C0	FASTA	537	58,063
	10 20 30 40 50 60 MAAVAVAAAA AALRARILQV SSKVNSSWQP ASSFSSSSVP TVKLFIDGKF IESKSDKWID 70 80 90 100 110 120 IHNPATNEVI GRVPESTKAE MDAAVSSCKR TFPAWADTSI LSRQQVLLRY QQLIKENLKE 130 140 150 160 170 180 IARLIMLEQG KTLADAEGDV FRGLQVVEHA CSVTSLMLGD TMPSITKDMD LYSYRLPLGV 190 200 210 220 230 240 CAGIAPFNFP AMIPLWMFPM AMVCGNTFLM KPSERVPGAT MLLAKLFQDS GAPDGTLNII 250 260 270 280 290 300 HGQHEAVNFI CDHPDIKAIS FVGSNQAGEY IFERGSRHGK RVQANMGAKN HGVVMPDANK 310 320 330 340 350 360 ENTLNQLVGA AFGAAGQRCM ALSTAILVGE AKKWLPELVE RAKKLRVNAG DQPGADLGPL 370 380 390 400 410 420 ITPQAKERVC NLIDSGTKEG ASILLDGRSI KVKGYENGNF VGPTIISNVK PNMTCYKEEI 430 440 450 460 470 480 FGPVLVVLET DTLDEAIKIV NDNPYGNGTA IFTTNGATAR KYSHLVDVGQ VGVNVPIPVP 490 500 510 520 530 LPMFSFTGSR ASFRGDTNFY GKQGIQFYTQ LKTITSQWKE EDASLSSPAV VMPTMGR

References

[1]

"Novel use of an iodo-myristyl-CoA analog identifies a semialdehyde dehydrogenase in bovine liver." Deichaite I., Berthiaume L., Peseckis S.M., Patton W.F., Resh M.D. J. Biol. Chem. 268:13738-13747(1993) [PubMed: 8514806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L08643 mRNA. Translation: AAA30650.1.
PIR	A46600.
RefSeq	NP_787005.1.
UniGene	Bt.98765
3D structure databases
HSSP	HSSP built from PDB template 1O9J based on UniProtKB Q28399.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000018469. Bos taurus. [Contig view]
GeneID	327692.
KEGG	bta:327692.
Phylogenomic databases
HOVERGEN	Q07536.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR010061. MeMal-semiAld_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMSA_BOVIN

Accession

Primary (citable) accession number: Q07536

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)