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Protein

Solute carrier family 25 member 38 homolog

Gene

HEM25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial carrier required for the biosynthesis of heme, possibly by facilitating 5-aminolevulinate (ALA) production. May act by importing glycine into mitochondria or by exchanging glycine for ALA across the mitochondrial inner membrane.UniRule annotation1 Publication

GO - Molecular functioni

  • glycine transmembrane transporter activity Source: SGD

GO - Biological processi

  • glycine import Source: SGD
  • heme biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29518-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 25 member 38 homologUniRule annotation
Alternative name(s):
Heme biosynthesis protein of SLC25 family1 Publication
Gene namesi
Name:HEM251 Publication
Ordered Locus Names:YDL119CImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL119C.
SGDiS000002277. HEM25.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei14 – 39Helical; Name=1UniRule annotationAdd BLAST26
Transmembranei62 – 88Helical; Name=2UniRule annotationAdd BLAST27
Transmembranei121 – 146Helical; Name=3UniRule annotationAdd BLAST26
Transmembranei174 – 197Helical; Name=4UniRule annotationAdd BLAST24
Transmembranei225 – 251Helical; Name=5UniRule annotationAdd BLAST27
Transmembranei280 – 298Helical; Name=6UniRule annotationAdd BLAST19

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-HAMAP
  • mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Yeasts grow well on the fermentable carbon source dextrose, but only poorly aerobically on glycerol, indicating a defect in respiration. Furthermore, the deletion strain is unable to reduce sodium nitroprusside, indicating that the defect is likely in heme biosynthesis. Nitroprusside reduction can be rescued by supplementation of the medium with either glycine or 5-aminolevulinate (ALA) (PubMed:19412178). In combination with a disruption of MCX1, abrogates mitochondrial respiration (PubMed:25957689).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002407071 – 307Solute carrier family 25 member 38 homologAdd BLAST307

Proteomic databases

MaxQBiQ07534.
PRIDEiQ07534.

Interactioni

Protein-protein interaction databases

BioGridi31944. 46 interactors.
MINTiMINT-4479341.

Structurei

3D structure databases

ProteinModelPortaliQ07534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati8 – 87Solcar 1Add BLAST80
Repeati115 – 199Solcar 2Add BLAST85
Repeati221 – 305Solcar 3Add BLAST85

Sequence similaritiesi

Belongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily. [View classification]UniRule annotation
Contains 3 Solcar repeats.UniRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075117.
HOGENOMiHOG000006978.
InParanoidiQ07534.
KOiK15118.
OMAiWYTEREA.
OrthoDBiEOG092C3NVU.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
HAMAPiMF_03064. SLC25A38. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
IPR030847. SLC25A38.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQATKPRN SSHLIGGFFG GLTSAVALQP LDLLKTRIQQ DKKATLWKNL
60 70 80 90 100
KEIDSPLQLW RGTLPSALRT SIGSALYLSC LNLMRSSLAK RRNAVPSLTN
110 120 130 140 150
DSNIVYNKSS SLPRLTMYEN LLTGAFARGL VGYITMPITV IKVRYESTLY
160 170 180 190 200
NYSSLKEAIT HIYTKEGLFG FFRGFGATCL RDAPYAGLYV LLYEKSKQLL
210 220 230 240 250
PMVLPSRFIH YNPEGGFTTY TSTTVNTTSA VLSASLATTV TAPFDTIKTR
260 270 280 290 300
MQLEPSKFTN SFNTFTSIVK NENVLKLFSG LSMRLARKAF SAGIAWGIYE

ELVKRFM
Length:307
Mass (Da):34,204
Last modified:November 1, 1996 - v1
Checksum:i7448D1559E157C17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74167 Genomic DNA. Translation: CAA98686.1.
AY692648 Genomic DNA. Translation: AAT92667.1.
BK006938 Genomic DNA. Translation: DAA11741.1.
PIRiS67662.
RefSeqiNP_010164.1. NM_001180178.1.

Genome annotation databases

EnsemblFungiiYDL119C; YDL119C; YDL119C.
GeneIDi851439.
KEGGisce:YDL119C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74167 Genomic DNA. Translation: CAA98686.1.
AY692648 Genomic DNA. Translation: AAT92667.1.
BK006938 Genomic DNA. Translation: DAA11741.1.
PIRiS67662.
RefSeqiNP_010164.1. NM_001180178.1.

3D structure databases

ProteinModelPortaliQ07534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31944. 46 interactors.
MINTiMINT-4479341.

Proteomic databases

MaxQBiQ07534.
PRIDEiQ07534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL119C; YDL119C; YDL119C.
GeneIDi851439.
KEGGisce:YDL119C.

Organism-specific databases

EuPathDBiFungiDB:YDL119C.
SGDiS000002277. HEM25.

Phylogenomic databases

GeneTreeiENSGT00550000075117.
HOGENOMiHOG000006978.
InParanoidiQ07534.
KOiK15118.
OMAiWYTEREA.
OrthoDBiEOG092C3NVU.

Enzyme and pathway databases

BioCyciYEAST:G3O-29518-MONOMER.

Miscellaneous databases

PROiQ07534.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
HAMAPiMF_03064. SLC25A38. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
IPR030847. SLC25A38.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS2538_YEAST
AccessioniPrimary (citable) accession number: Q07534
Secondary accession number(s): D6VRN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1550 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.