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Q07533

- CYK3_YEAST

UniProt

Q07533 - CYK3_YEAST

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Protein

Cytokinesis protein 3

Gene

CYK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in cytokinesis by recruiting INN1 to the bud neck. Cooperates with INN1 to stimulate the synthesis of the primary septum (PS) by CHS2.3 Publications

GO - Molecular functioni

  1. enzyme regulator activity Source: SGD

GO - Biological processi

  1. barrier septum assembly Source: SGD
  2. regulation of catalytic activity Source: GOC
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciYEAST:G3O-29517-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokinesis protein 3
Gene namesi
Name:CYK3
Ordered Locus Names:YDL117W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL117w.
SGDiS000002275. CYK3.

Subcellular locationi

Cytoplasm. Bud neck
Note: Found in association with the actin ring and the cortex at the mother-bud neck.

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud neck contractile ring Source: SGD
  3. HICS complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 885885Cytokinesis protein 3PRO_0000079751Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei209 – 2091Phosphoserine1 Publication
Modified residuei292 – 2921Phosphoserine1 Publication
Modified residuei313 – 3131Phosphoserine2 Publications
Modified residuei354 – 3541Phosphoserine1 Publication
Modified residuei391 – 3911Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ07533.
PaxDbiQ07533.
PRIDEiQ07533.

Expressioni

Gene expression databases

GenevestigatoriQ07533.

Interactioni

Subunit structurei

Interacts with INN1.2 Publications

Protein-protein interaction databases

BioGridi31945. 152 interactions.
DIPiDIP-6625N.
IntActiQ07533. 21 interactions.
MINTiMINT-598450.
STRINGi4932.YDL117W.

Structurei

3D structure databases

ProteinModelPortaliQ07533.
SMRiQ07533. Positions 12-69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 7062SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CYK3 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiCOG5279.
HOGENOMiHOG000112129.
InParanoidiQ07533.
OMAiCTPYELT.
OrthoDBiEOG76TB0N.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR002931. Transglutaminase-like.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
SM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07533 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATNLTSLKP PFKVKARYGW SGQTKGDLGF LEGDIMEVTR IAGSWFYGKL
60 70 80 90 100
LRNKKCSGYF PHNFVILLEE RLNSSTENGR QPSKIVESFE KSNKVVIPPV
110 120 130 140 150
PSRYSDERPR PKKKLSSSMP NSPKKPVDSL TKARKAKSKE MVNEKNIYNT
160 170 180 190 200
QSSRHHNNSA PNLPLASHSK PQVRNFEESM NNPLPPLPPL PDLDNMRKTD
210 220 230 240 250
KRAPKKSYSA NDLHMARSSR EYNYYKDNQK FYDGFIPEKR YSLEEDSISS
260 270 280 290 300
GLFSNSQYLN DSACSSENSF ALMSDFSATS AGSFARHKYA QSFSDSLQRS
310 320 330 340 350
QNANGCSTKI NDSQEFGDSN ASSRNGKMGD ILRKIIIPKR NTNIYSSSVS
360 370 380 390 400
SPKSPKAYPK LPDIQNLNLS ATPDEARDWI AVKCHLNRAR TLTKYDKHPR
410 420 430 440 450
YMRALEENRD LILHPQDSIY NGLNTNEVKG NTKPGLVDVE LAELNIEYID
460 470 480 490 500
KMTWKRCIRD GTMTLDSWAQ TTFSARYSTV LEKLRGIYIF CTEMFALTDD
510 520 530 540 550
NGTSDFSAEP QNLEKILYRK HCTPYELTWL FKKLANSLGI TCEIVIGFLK
560 570 580 590 600
TPSAINWEFK YNHCWLRILV NKEWRFIDVI LGNVTNPIHE FVNNRKIKKA
610 620 630 640 650
ENSYFLMAPL EMIYTHIPPR EFEQHIVPSI DQLSALYLPL VFPSFFKNEL
660 670 680 690 700
KLYKFSTALS FLEDSEIYEC SLEIPNDVEV FASVVIPTDN EEASSAYRNM
710 720 730 740 750
ELALTQIKKQ KAESGRRIAL IKAVLPPNVN KGSLYIHSGV RGTQTSIANI
760 770 780 790 800
HPLSMMVPLT HKGSNMKYEF VIKIPSESIQ KIELYIVEPQ SRYLFVGNEY
810 820 830 840 850
SFEVIQSPSD GIVYSSDEGP NQNRKQPMAI KSPSGRVHEL VKSDPHFPYG
860 870 880
TWKGSIKIKE PGVWSALVIA DSGIGWSVFA EWLCV
Length:885
Mass (Da):100,621
Last modified:November 1, 1996 - v1
Checksum:iA1B7FD5B7076EC7F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74165 Genomic DNA. Translation: CAA98685.1.
BK006938 Genomic DNA. Translation: DAA11743.1.
PIRiS67660.
RefSeqiNP_010166.1. NM_001180176.1.

Genome annotation databases

EnsemblFungiiYDL117W; YDL117W; YDL117W.
GeneIDi851440.
KEGGisce:YDL117W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74165 Genomic DNA. Translation: CAA98685.1 .
BK006938 Genomic DNA. Translation: DAA11743.1 .
PIRi S67660.
RefSeqi NP_010166.1. NM_001180176.1.

3D structure databases

ProteinModelPortali Q07533.
SMRi Q07533. Positions 12-69.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31945. 152 interactions.
DIPi DIP-6625N.
IntActi Q07533. 21 interactions.
MINTi MINT-598450.
STRINGi 4932.YDL117W.

Proteomic databases

MaxQBi Q07533.
PaxDbi Q07533.
PRIDEi Q07533.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL117W ; YDL117W ; YDL117W .
GeneIDi 851440.
KEGGi sce:YDL117W.

Organism-specific databases

CYGDi YDL117w.
SGDi S000002275. CYK3.

Phylogenomic databases

eggNOGi COG5279.
HOGENOMi HOG000112129.
InParanoidi Q07533.
OMAi CTPYELT.
OrthoDBi EOG76TB0N.

Enzyme and pathway databases

BioCyci YEAST:G3O-29517-MONOMER.

Miscellaneous databases

NextBioi 968681.

Gene expression databases

Genevestigatori Q07533.

Family and domain databases

InterProi IPR001452. SH3_domain.
IPR002931. Transglutaminase-like.
[Graphical view ]
SMARTi SM00326. SH3. 1 hit.
SM00460. TGc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Cyk3, a novel SH3-domain protein, affects cytokinesis in yeast."
    Korinek W.S., Bi E., Epp J.A., Wang L., Ho J., Chant J.
    Curr. Biol. 10:947-950(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209 AND SER-354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-391, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae."
    Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E.
    J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
  10. "Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck."
    Jendretzki A., Ciklic I., Rodicio R., Schmitz H.P., Heinisch J.J.
    Mol. Genet. Genomics 282:437-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292 AND SER-313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCYK3_YEAST
AccessioniPrimary (citable) accession number: Q07533
Secondary accession number(s): D6VRN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3