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Q07533

- CYK3_YEAST

UniProt

Q07533 - CYK3_YEAST

Protein

Cytokinesis protein 3

Gene

CYK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in cytokinesis by recruiting INN1 to the bud neck. Cooperates with INN1 to stimulate the synthesis of the primary septum (PS) by CHS2.3 Publications

    GO - Molecular functioni

    1. enzyme regulator activity Source: SGD

    GO - Biological processi

    1. barrier septum assembly Source: SGD
    2. regulation of catalytic activity Source: GOC

    Keywords - Biological processi

    Cell cycle, Cell division

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29517-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytokinesis protein 3
    Gene namesi
    Name:CYK3
    Ordered Locus Names:YDL117W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL117w.
    SGDiS000002275. CYK3.

    Subcellular locationi

    Cytoplasm. Bud neck
    Note: Found in association with the actin ring and the cortex at the mother-bud neck.

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud neck contractile ring Source: SGD
    3. HICS complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 885885Cytokinesis protein 3PRO_0000079751Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei209 – 2091Phosphoserine1 Publication
    Modified residuei292 – 2921Phosphoserine1 Publication
    Modified residuei313 – 3131Phosphoserine2 Publications
    Modified residuei354 – 3541Phosphoserine1 Publication
    Modified residuei391 – 3911Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ07533.
    PaxDbiQ07533.
    PRIDEiQ07533.

    Expressioni

    Gene expression databases

    GenevestigatoriQ07533.

    Interactioni

    Subunit structurei

    Interacts with INN1.2 Publications

    Protein-protein interaction databases

    BioGridi31945. 151 interactions.
    DIPiDIP-6625N.
    IntActiQ07533. 21 interactions.
    MINTiMINT-598450.
    STRINGi4932.YDL117W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07533.
    SMRiQ07533. Positions 12-69.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 7062SH3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CYK3 family.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiCOG5279.
    HOGENOMiHOG000112129.
    OMAiCTPYELT.
    OrthoDBiEOG76TB0N.

    Family and domain databases

    InterProiIPR001452. SH3_domain.
    IPR002931. Transglutaminase-like.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    SM00460. TGc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q07533-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATNLTSLKP PFKVKARYGW SGQTKGDLGF LEGDIMEVTR IAGSWFYGKL    50
    LRNKKCSGYF PHNFVILLEE RLNSSTENGR QPSKIVESFE KSNKVVIPPV 100
    PSRYSDERPR PKKKLSSSMP NSPKKPVDSL TKARKAKSKE MVNEKNIYNT 150
    QSSRHHNNSA PNLPLASHSK PQVRNFEESM NNPLPPLPPL PDLDNMRKTD 200
    KRAPKKSYSA NDLHMARSSR EYNYYKDNQK FYDGFIPEKR YSLEEDSISS 250
    GLFSNSQYLN DSACSSENSF ALMSDFSATS AGSFARHKYA QSFSDSLQRS 300
    QNANGCSTKI NDSQEFGDSN ASSRNGKMGD ILRKIIIPKR NTNIYSSSVS 350
    SPKSPKAYPK LPDIQNLNLS ATPDEARDWI AVKCHLNRAR TLTKYDKHPR 400
    YMRALEENRD LILHPQDSIY NGLNTNEVKG NTKPGLVDVE LAELNIEYID 450
    KMTWKRCIRD GTMTLDSWAQ TTFSARYSTV LEKLRGIYIF CTEMFALTDD 500
    NGTSDFSAEP QNLEKILYRK HCTPYELTWL FKKLANSLGI TCEIVIGFLK 550
    TPSAINWEFK YNHCWLRILV NKEWRFIDVI LGNVTNPIHE FVNNRKIKKA 600
    ENSYFLMAPL EMIYTHIPPR EFEQHIVPSI DQLSALYLPL VFPSFFKNEL 650
    KLYKFSTALS FLEDSEIYEC SLEIPNDVEV FASVVIPTDN EEASSAYRNM 700
    ELALTQIKKQ KAESGRRIAL IKAVLPPNVN KGSLYIHSGV RGTQTSIANI 750
    HPLSMMVPLT HKGSNMKYEF VIKIPSESIQ KIELYIVEPQ SRYLFVGNEY 800
    SFEVIQSPSD GIVYSSDEGP NQNRKQPMAI KSPSGRVHEL VKSDPHFPYG 850
    TWKGSIKIKE PGVWSALVIA DSGIGWSVFA EWLCV 885
    Length:885
    Mass (Da):100,621
    Last modified:November 1, 1996 - v1
    Checksum:iA1B7FD5B7076EC7F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74165 Genomic DNA. Translation: CAA98685.1.
    BK006938 Genomic DNA. Translation: DAA11743.1.
    PIRiS67660.
    RefSeqiNP_010166.1. NM_001180176.1.

    Genome annotation databases

    EnsemblFungiiYDL117W; YDL117W; YDL117W.
    GeneIDi851440.
    KEGGisce:YDL117W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74165 Genomic DNA. Translation: CAA98685.1 .
    BK006938 Genomic DNA. Translation: DAA11743.1 .
    PIRi S67660.
    RefSeqi NP_010166.1. NM_001180176.1.

    3D structure databases

    ProteinModelPortali Q07533.
    SMRi Q07533. Positions 12-69.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31945. 151 interactions.
    DIPi DIP-6625N.
    IntActi Q07533. 21 interactions.
    MINTi MINT-598450.
    STRINGi 4932.YDL117W.

    Proteomic databases

    MaxQBi Q07533.
    PaxDbi Q07533.
    PRIDEi Q07533.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL117W ; YDL117W ; YDL117W .
    GeneIDi 851440.
    KEGGi sce:YDL117W.

    Organism-specific databases

    CYGDi YDL117w.
    SGDi S000002275. CYK3.

    Phylogenomic databases

    eggNOGi COG5279.
    HOGENOMi HOG000112129.
    OMAi CTPYELT.
    OrthoDBi EOG76TB0N.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29517-MONOMER.

    Miscellaneous databases

    NextBioi 968681.

    Gene expression databases

    Genevestigatori Q07533.

    Family and domain databases

    InterProi IPR001452. SH3_domain.
    IPR002931. Transglutaminase-like.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    SM00460. TGc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Cyk3, a novel SH3-domain protein, affects cytokinesis in yeast."
      Korinek W.S., Bi E., Epp J.A., Wang L., Ho J., Chant J.
      Curr. Biol. 10:947-950(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209 AND SER-354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-391, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae."
      Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E.
      J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
    10. "Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck."
      Jendretzki A., Ciklic I., Rodicio R., Schmitz H.P., Heinisch J.J.
      Mol. Genet. Genomics 282:437-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292 AND SER-313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCYK3_YEAST
    AccessioniPrimary (citable) accession number: Q07533
    Secondary accession number(s): D6VRN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 377 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3