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Q07533 (CYK3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytokinesis protein 3
Gene names
Name:CYK3
Ordered Locus Names:YDL117W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length885 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cytokinesis by recruiting INN1 to the bud neck. Cooperates with INN1 to stimulate the synthesis of the primary septum (PS) by CHS2. Ref.3 Ref.9 Ref.10

Subunit structure

Interacts with INN1. Ref.9 Ref.10

Subcellular location

Cytoplasm. Bud neck. Note: Found in association with the actin ring and the cortex at the mother-bud neck. Ref.3 Ref.4 Ref.9 Ref.10

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CYK3 family.

Contains 1 SH3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 885885Cytokinesis protein 3
PRO_0000079751

Regions

Domain9 – 7062SH3

Amino acid modifications

Modified residue2091Phosphoserine Ref.6
Modified residue2921Phosphoserine Ref.11
Modified residue3131Phosphoserine Ref.8 Ref.11
Modified residue3541Phosphoserine Ref.6
Modified residue3911Phosphothreonine Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q07533 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A1B7FD5B7076EC7F

FASTA885100,621
        10         20         30         40         50         60 
MATNLTSLKP PFKVKARYGW SGQTKGDLGF LEGDIMEVTR IAGSWFYGKL LRNKKCSGYF 

        70         80         90        100        110        120 
PHNFVILLEE RLNSSTENGR QPSKIVESFE KSNKVVIPPV PSRYSDERPR PKKKLSSSMP 

       130        140        150        160        170        180 
NSPKKPVDSL TKARKAKSKE MVNEKNIYNT QSSRHHNNSA PNLPLASHSK PQVRNFEESM 

       190        200        210        220        230        240 
NNPLPPLPPL PDLDNMRKTD KRAPKKSYSA NDLHMARSSR EYNYYKDNQK FYDGFIPEKR 

       250        260        270        280        290        300 
YSLEEDSISS GLFSNSQYLN DSACSSENSF ALMSDFSATS AGSFARHKYA QSFSDSLQRS 

       310        320        330        340        350        360 
QNANGCSTKI NDSQEFGDSN ASSRNGKMGD ILRKIIIPKR NTNIYSSSVS SPKSPKAYPK 

       370        380        390        400        410        420 
LPDIQNLNLS ATPDEARDWI AVKCHLNRAR TLTKYDKHPR YMRALEENRD LILHPQDSIY 

       430        440        450        460        470        480 
NGLNTNEVKG NTKPGLVDVE LAELNIEYID KMTWKRCIRD GTMTLDSWAQ TTFSARYSTV 

       490        500        510        520        530        540 
LEKLRGIYIF CTEMFALTDD NGTSDFSAEP QNLEKILYRK HCTPYELTWL FKKLANSLGI 

       550        560        570        580        590        600 
TCEIVIGFLK TPSAINWEFK YNHCWLRILV NKEWRFIDVI LGNVTNPIHE FVNNRKIKKA 

       610        620        630        640        650        660 
ENSYFLMAPL EMIYTHIPPR EFEQHIVPSI DQLSALYLPL VFPSFFKNEL KLYKFSTALS 

       670        680        690        700        710        720 
FLEDSEIYEC SLEIPNDVEV FASVVIPTDN EEASSAYRNM ELALTQIKKQ KAESGRRIAL 

       730        740        750        760        770        780 
IKAVLPPNVN KGSLYIHSGV RGTQTSIANI HPLSMMVPLT HKGSNMKYEF VIKIPSESIQ 

       790        800        810        820        830        840 
KIELYIVEPQ SRYLFVGNEY SFEVIQSPSD GIVYSSDEGP NQNRKQPMAI KSPSGRVHEL 

       850        860        870        880 
VKSDPHFPYG TWKGSIKIKE PGVWSALVIA DSGIGWSVFA EWLCV 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Cyk3, a novel SH3-domain protein, affects cytokinesis in yeast."
Korinek W.S., Bi E., Epp J.A., Wang L., Ho J., Chant J.
Curr. Biol. 10:947-950(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209 AND SER-354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-391, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae."
Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E.
J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
[10]"Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck."
Jendretzki A., Ciklic I., Rodicio R., Schmitz H.P., Heinisch J.J.
Mol. Genet. Genomics 282:437-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292 AND SER-313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z74165 Genomic DNA. Translation: CAA98685.1.
BK006938 Genomic DNA. Translation: DAA11743.1.
PIRS67660.
RefSeqNP_010166.1. NM_001180176.1.

3D structure databases

ProteinModelPortalQ07533.
SMRQ07533. Positions 12-69.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31945. 150 interactions.
DIPDIP-6625N.
IntActQ07533. 21 interactions.
MINTMINT-598450.
STRING4932.YDL117W.

Proteomic databases

MaxQBQ07533.
PaxDbQ07533.
PRIDEQ07533.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL117W; YDL117W; YDL117W.
GeneID851440.
KEGGsce:YDL117W.

Organism-specific databases

CYGDYDL117w.
SGDS000002275. CYK3.

Phylogenomic databases

eggNOGCOG5279.
HOGENOMHOG000112129.
OMACTPYELT.
OrthoDBEOG76TB0N.

Enzyme and pathway databases

BioCycYEAST:G3O-29517-MONOMER.

Gene expression databases

GenevestigatorQ07533.

Family and domain databases

InterProIPR001452. SH3_domain.
IPR002931. Transglutaminase-like.
[Graphical view]
SMARTSM00326. SH3. 1 hit.
SM00460. TGc. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968681.

Entry information

Entry nameCYK3_YEAST
AccessionPrimary (citable) accession number: Q07533
Secondary accession number(s): D6VRN3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families