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Q07528 (ATG20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Autophagy-related protein 20
Alternative name(s):
Cytoplasm to vacuole targeting protein 20
Sorting nexin-42
Gene names
Name:ATG20
Synonyms:CVT20, SNX42
Ordered Locus Names:YDL113C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for cytoplasm to vacuole transport (Cvt), pexophagy and mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Functions in protein retrieval from the endocytic pathway. Required for proper sorting of the v-SNARE protein SNC1. Ref.3 Ref.6 Ref.10 Ref.11 Ref.12 Ref.15 Ref.16

Subunit structure

Forms a complex with SNX4 and ATG17.

Subcellular location

Endosome membrane; Peripheral membrane protein. Preautophagosomal structure membrane; Peripheral membrane protein Ref.3 Ref.7 Ref.9.

Domain

The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization of ATG20 to the perivacuolar punctate structures.

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the sorting nexin family.

Contains 1 PX (phox homology) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SNX4P470577EBI-36894,EBI-17610
YIP1P530392EBI-36894,EBI-25295

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 640640Autophagy-related protein 20
PRO_0000213824

Regions

Domain140 – 301162PX
Coiled coil475 – 51238 Potential
Coiled coil562 – 59332 Potential

Sites

Binding site1921Phosphatidylinositol 3-phosphate By similarity
Binding site1941Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site2181Phosphatidylinositol 3-phosphate By similarity
Binding site2671Phosphatidylinositol 3-phosphate By similarity

Amino acid modifications

Modified residue451Phosphoserine Ref.4
Modified residue491Phosphoserine Ref.4
Modified residue3611Phosphoserine Ref.13 Ref.14
Modified residue3631Phosphoserine Ref.13 Ref.14
Modified residue3651Phosphothreonine Ref.13 Ref.14

Experimental info

Mutagenesis1931Y → A: Abolishes the intracellular punctate localization and decreases the cytoplasm to vacuole transport. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q07528 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 160977A8BECB6D93

FASTA64072,546
        10         20         30         40         50         60 
MSDLNDVQEN AKLNSETRNT GKAEPPHGTT EYVAEAEISK NGVGSPKKSP KKGKVGKGDN 

        70         80         90        100        110        120 
NKVETELVHT ALLEKDNPFM EEGPTGFTKS ALLEIPGMRS HNLKNPNEDY EDDSEGLLPL 

       130        140        150        160        170        180 
NQESNAETCR TSLSGSINSM NGETSASEEP SVSNRKKSAR IHILEAKRVS EGQGRAYIAY 

       190        200        210        220        230        240 
VIQFENSTVQ RRYSDFESLR SILIRLFPMT LIPPIPEKQS IKNYGKSITG SSSKYLLPSE 

       250        260        270        280        290        300 
GSGSVDLSLS VIHASVNNSD EKLIRHRIRM LTEFLNKLLT NEEITKTSII TDFLDPNNHN 

       310        320        330        340        350        360 
WHEFVNSSST FSSLPKSILQ CNPLDPTNTT RIHAMLPIPG SSSQLLLNKE SNDKKMDKER 

       370        380        390        400        410        420 
SKSFTNIEQD YKQYENLLDN GIYKYNRRTT KTYHDLKSDY NEIGEVFAQF AHEQAQVGEL 

       430        440        450        460        470        480 
AEQLSYLSNA FSGSSISLEK LVGRLYYNIN EPLNESVHMA TSARELIKYR KLKYLQNEMI 

       490        500        510        520        530        540 
KKSLNSKRAQ LEKLEAQNNE YKDVDKIIDN EMSKSHTINL ERPNNNTGSG GKSYGGKLFN 

       550        560        570        580        590        600 
GFNKLASMVK DSVKYQETDP HTASINLKKE IEQLSESLEV TENDLEVISK VIKNDQLPKF 

       610        620        630        640 
SKEREVDLSE ILKHYSRYMR NYARQNLEIW KEVKRHQDFA

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Cooperative binding of the cytoplasm to vacuole targeting pathway proteins, Cvt13 and Cvt20, to phosphatidylinositol 3-phosphate at the pre-autophagosomal structure is required for selective autophagy."
Nice D.C. III, Sato T.K., Stromhaug P.E., Emr S.D., Klionsky D.J.
J. Biol. Chem. 277:30198-30207(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG17 AND SNX4, MUTAGENESIS OF TYR-193.
[4]"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae."
Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M.
Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND SER-49, MASS SPECTROMETRY.
Strain: 2124.
[5]"A unified nomenclature for yeast autophagy-related genes."
Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.
Dev. Cell 5:539-545(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[6]"Retromer and the sorting nexins Snx4/41/42 mediate distinct retrieval pathways from yeast endosomes."
Hettema E.H., Lewis M.J., Black M.W., Pelham H.R.B.
EMBO J. 22:548-557(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SNX4.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway."
Yorimitsu T., Klionsky D.J.
Mol. Biol. Cell 16:1593-1605(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ATG11.
[10]"Atg17 regulates the magnitude of the autophagic response."
Cheong H., Yorimitsu T., Reggiori F., Legakis J.E., Wang C.W., Klionsky D.J.
Mol. Biol. Cell 16:3438-3453(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"A yeast model system for functional analysis of the Niemann-Pick type C protein 1 homolog, Ncr1p."
Berger A.C., Hanson P.K., Wylie Nichols J., Corbett A.H.
Traffic 6:907-917(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response."
Bernales S., McDonald K.L., Walter P.
PLoS Biol. 4:E423-E423(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361; SER-363 AND THR-365, MASS SPECTROMETRY.
[14]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361; SER-363 AND THR-365, MASS SPECTROMETRY.
[15]"A genomic screen for yeast mutants defective in selective mitochondria autophagy."
Kanki T., Wang K., Baba M., Bartholomew C.R., Lynch-Day M.A., Du Z., Geng J., Mao K., Yang Z., Yen W.L., Klionsky D.J.
Mol. Biol. Cell 20:4730-4738(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[16]"Membrane delivery to the yeast autophagosome from the Golgi-endosomal system."
Ohashi Y., Munro S.
Mol. Biol. Cell 21:3998-4008(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z74161 Genomic DNA. Translation: CAA98681.1.
BK006938 Genomic DNA. Translation: DAA11747.1.
PIRS67656.
RefSeqNP_010170.1. NM_001180172.1.

3D structure databases

ProteinModelPortalQ07528.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1593N.
IntActQ07528. 11 interactions.
MINTMINT-402385.
STRING4932.YDL113C.

Proteomic databases

PaxDbQ07528.
PeptideAtlasQ07528.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL113C; YDL113C; YDL113C.
GeneID851445.
KEGGsce:YDL113C.

Organism-specific databases

CYGDYDL113c.
SGDS000002271. ATG20.

Phylogenomic databases

eggNOGCOG5391.
GeneTreeENSGT00530000066389.
HOGENOMHOG000034139.
OMAIRHRIRM.
OrthoDBEOG4NCQNF.

Gene expression databases

GenevestigatorQ07528.
GermOnlineYDL113C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D3.30.1520.10. 1 hit.
InterProIPR001683. Phox.
[Graphical view]
PfamPF00787. PX. 1 hit.
[Graphical view]
SMARTSM00312. PX. 1 hit.
[Graphical view]
SUPFAMSSF64268. PX. 1 hit.
PROSITEPS50195. PX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968693.

Entry information

Entry nameATG20_YEAST
AccessionPrimary (citable) accession number: Q07528
Secondary accession number(s): D6VRN7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents