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Protein

Autophagy-related protein 20

Gene

ATG20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for cytoplasm to vacuole transport (Cvt), pexophagy and mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Functions in protein retrieval from the endocytic pathway. Required for proper sorting of the v-SNARE protein SNC1.7 Publications

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei194Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei218Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei267Phosphatidylinositol 3-phosphateBy similarity1

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  • autophagy of mitochondrion Source: SGD
  • early endosome to Golgi transport Source: SGD
  • endocytosis Source: GO_Central
  • macroautophagy Source: SGD
  • protein localization by the Cvt pathway Source: SGD
  • protein transport Source: UniProtKB-KW
  • vesicle organization Source: GO_Central

Keywordsi

Biological processAutophagy, Protein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29513-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 20
Alternative name(s):
Cytoplasm to vacuole targeting protein 20
Sorting nexin-42
Gene namesi
Name:ATG20
Synonyms:CVT20, SNX42
Ordered Locus Names:YDL113C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL113C
SGDiS000002271 ATG20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi193Y → A: Abolishes the intracellular punctate localization and decreases the cytoplasm to vacuole transport. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002138242 – 640Autophagy-related protein 20Add BLAST639

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei361PhosphoserineCombined sources1
Modified residuei363PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ07528
PaxDbiQ07528
PRIDEiQ07528

PTM databases

iPTMnetiQ07528

Interactioni

Subunit structurei

Forms a complex with SNX4 and ATG17.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31949, 105 interactors
DIPiDIP-1593N
IntActiQ07528, 13 interactors
MINTiQ07528
STRINGi4932.YDL113C

Structurei

3D structure databases

ProteinModelPortaliQ07528
SMRiQ07528
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 301PXPROSITE-ProRule annotationAdd BLAST162

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili475 – 512Sequence analysisAdd BLAST38
Coiled coili562 – 593Sequence analysisAdd BLAST32

Domaini

The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization of ATG20 to the perivacuolar punctate structures.

Sequence similaritiesi

Belongs to the sorting nexin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000066389
HOGENOMiHOG000034139
InParanoidiQ07528
KOiK21996
OMAiINTRRRY
OrthoDBiEOG092C1UWN

Family and domain databases

InterProiView protein in InterPro
IPR001683 Phox
IPR036871 PX_dom_sf
PfamiView protein in Pfam
PF00787 PX, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF64268 SSF64268, 2 hits
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLNDVQEN AKLNSETRNT GKAEPPHGTT EYVAEAEISK NGVGSPKKSP
60 70 80 90 100
KKGKVGKGDN NKVETELVHT ALLEKDNPFM EEGPTGFTKS ALLEIPGMRS
110 120 130 140 150
HNLKNPNEDY EDDSEGLLPL NQESNAETCR TSLSGSINSM NGETSASEEP
160 170 180 190 200
SVSNRKKSAR IHILEAKRVS EGQGRAYIAY VIQFENSTVQ RRYSDFESLR
210 220 230 240 250
SILIRLFPMT LIPPIPEKQS IKNYGKSITG SSSKYLLPSE GSGSVDLSLS
260 270 280 290 300
VIHASVNNSD EKLIRHRIRM LTEFLNKLLT NEEITKTSII TDFLDPNNHN
310 320 330 340 350
WHEFVNSSST FSSLPKSILQ CNPLDPTNTT RIHAMLPIPG SSSQLLLNKE
360 370 380 390 400
SNDKKMDKER SKSFTNIEQD YKQYENLLDN GIYKYNRRTT KTYHDLKSDY
410 420 430 440 450
NEIGEVFAQF AHEQAQVGEL AEQLSYLSNA FSGSSISLEK LVGRLYYNIN
460 470 480 490 500
EPLNESVHMA TSARELIKYR KLKYLQNEMI KKSLNSKRAQ LEKLEAQNNE
510 520 530 540 550
YKDVDKIIDN EMSKSHTINL ERPNNNTGSG GKSYGGKLFN GFNKLASMVK
560 570 580 590 600
DSVKYQETDP HTASINLKKE IEQLSESLEV TENDLEVISK VIKNDQLPKF
610 620 630 640
SKEREVDLSE ILKHYSRYMR NYARQNLEIW KEVKRHQDFA
Length:640
Mass (Da):72,546
Last modified:November 1, 1996 - v1
Checksum:i160977A8BECB6D93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74161 Genomic DNA Translation: CAA98681.1
BK006938 Genomic DNA Translation: DAA11747.1
PIRiS67656
RefSeqiNP_010170.1, NM_001180172.1

Genome annotation databases

EnsemblFungiiYDL113C; YDL113C; YDL113C
GeneIDi851445
KEGGisce:YDL113C

Similar proteinsi

Entry informationi

Entry nameiATG20_YEAST
AccessioniPrimary (citable) accession number: Q07528
Secondary accession number(s): D6VRN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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