Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q07528

- ATG20_YEAST

UniProt

Q07528 - ATG20_YEAST

Protein

Autophagy-related protein 20

Gene

ATG20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for cytoplasm to vacuole transport (Cvt), pexophagy and mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Functions in protein retrieval from the endocytic pathway. Required for proper sorting of the v-SNARE protein SNC1.7 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei192 – 1921Phosphatidylinositol 3-phosphateBy similarity
    Binding sitei194 – 1941Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei218 – 2181Phosphatidylinositol 3-phosphateBy similarity
    Binding sitei267 – 2671Phosphatidylinositol 3-phosphateBy similarity

    GO - Molecular functioni

    1. phosphatidylinositol-3-phosphate binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. CVT pathway Source: SGD
    2. early endosome to Golgi transport Source: SGD
    3. macroautophagy Source: SGD
    4. mitochondrion degradation Source: SGD

    Keywords - Biological processi

    Autophagy, Protein transport, Transport

    Keywords - Ligandi

    Lipid-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29513-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Autophagy-related protein 20
    Alternative name(s):
    Cytoplasm to vacuole targeting protein 20
    Sorting nexin-42
    Gene namesi
    Name:ATG20
    Synonyms:CVT20, SNX42
    Ordered Locus Names:YDL113C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL113c.
    SGDiS000002271. ATG20.

    Subcellular locationi

    GO - Cellular componenti

    1. endosome Source: SGD
    2. endosome membrane Source: UniProtKB-SubCell
    3. extrinsic component of membrane Source: SGD
    4. pre-autophagosomal structure Source: SGD
    5. pre-autophagosomal structure membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi193 – 1931Y → A: Abolishes the intracellular punctate localization and decreases the cytoplasm to vacuole transport. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 640639Autophagy-related protein 20PRO_0000213824Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei361 – 3611Phosphoserine1 Publication
    Modified residuei363 – 3631Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ07528.
    PaxDbiQ07528.
    PeptideAtlasiQ07528.

    Expressioni

    Gene expression databases

    GenevestigatoriQ07528.

    Interactioni

    Subunit structurei

    Forms a complex with SNX4 and ATG17.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SNX4P470577EBI-36894,EBI-17610
    YIP1P530392EBI-36894,EBI-25295

    Protein-protein interaction databases

    BioGridi31949. 49 interactions.
    DIPiDIP-1593N.
    IntActiQ07528. 10 interactions.
    MINTiMINT-402385.
    STRINGi4932.YDL113C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07528.
    SMRiQ07528. Positions 169-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini140 – 301162PXPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili475 – 51238Sequence AnalysisAdd
    BLAST
    Coiled coili562 – 59332Sequence AnalysisAdd
    BLAST

    Domaini

    The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization of ATG20 to the perivacuolar punctate structures.

    Sequence similaritiesi

    Belongs to the sorting nexin family.Curated
    Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5391.
    GeneTreeiENSGT00530000066389.
    HOGENOMiHOG000034139.
    OMAiSARIHIL.
    OrthoDBiEOG7M0P28.

    Family and domain databases

    Gene3Di3.30.1520.10. 1 hit.
    InterProiIPR001683. Phox.
    [Graphical view]
    PfamiPF00787. PX. 1 hit.
    [Graphical view]
    SMARTiSM00312. PX. 1 hit.
    [Graphical view]
    SUPFAMiSSF64268. SSF64268. 2 hits.
    PROSITEiPS50195. PX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q07528-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDLNDVQEN AKLNSETRNT GKAEPPHGTT EYVAEAEISK NGVGSPKKSP    50
    KKGKVGKGDN NKVETELVHT ALLEKDNPFM EEGPTGFTKS ALLEIPGMRS 100
    HNLKNPNEDY EDDSEGLLPL NQESNAETCR TSLSGSINSM NGETSASEEP 150
    SVSNRKKSAR IHILEAKRVS EGQGRAYIAY VIQFENSTVQ RRYSDFESLR 200
    SILIRLFPMT LIPPIPEKQS IKNYGKSITG SSSKYLLPSE GSGSVDLSLS 250
    VIHASVNNSD EKLIRHRIRM LTEFLNKLLT NEEITKTSII TDFLDPNNHN 300
    WHEFVNSSST FSSLPKSILQ CNPLDPTNTT RIHAMLPIPG SSSQLLLNKE 350
    SNDKKMDKER SKSFTNIEQD YKQYENLLDN GIYKYNRRTT KTYHDLKSDY 400
    NEIGEVFAQF AHEQAQVGEL AEQLSYLSNA FSGSSISLEK LVGRLYYNIN 450
    EPLNESVHMA TSARELIKYR KLKYLQNEMI KKSLNSKRAQ LEKLEAQNNE 500
    YKDVDKIIDN EMSKSHTINL ERPNNNTGSG GKSYGGKLFN GFNKLASMVK 550
    DSVKYQETDP HTASINLKKE IEQLSESLEV TENDLEVISK VIKNDQLPKF 600
    SKEREVDLSE ILKHYSRYMR NYARQNLEIW KEVKRHQDFA 640
    Length:640
    Mass (Da):72,546
    Last modified:November 1, 1996 - v1
    Checksum:i160977A8BECB6D93
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74161 Genomic DNA. Translation: CAA98681.1.
    BK006938 Genomic DNA. Translation: DAA11747.1.
    PIRiS67656.
    RefSeqiNP_010170.1. NM_001180172.1.

    Genome annotation databases

    EnsemblFungiiYDL113C; YDL113C; YDL113C.
    GeneIDi851445.
    KEGGisce:YDL113C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74161 Genomic DNA. Translation: CAA98681.1 .
    BK006938 Genomic DNA. Translation: DAA11747.1 .
    PIRi S67656.
    RefSeqi NP_010170.1. NM_001180172.1.

    3D structure databases

    ProteinModelPortali Q07528.
    SMRi Q07528. Positions 169-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31949. 49 interactions.
    DIPi DIP-1593N.
    IntActi Q07528. 10 interactions.
    MINTi MINT-402385.
    STRINGi 4932.YDL113C.

    Proteomic databases

    MaxQBi Q07528.
    PaxDbi Q07528.
    PeptideAtlasi Q07528.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL113C ; YDL113C ; YDL113C .
    GeneIDi 851445.
    KEGGi sce:YDL113C.

    Organism-specific databases

    CYGDi YDL113c.
    SGDi S000002271. ATG20.

    Phylogenomic databases

    eggNOGi COG5391.
    GeneTreei ENSGT00530000066389.
    HOGENOMi HOG000034139.
    OMAi SARIHIL.
    OrthoDBi EOG7M0P28.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29513-MONOMER.

    Miscellaneous databases

    NextBioi 968693.

    Gene expression databases

    Genevestigatori Q07528.

    Family and domain databases

    Gene3Di 3.30.1520.10. 1 hit.
    InterProi IPR001683. Phox.
    [Graphical view ]
    Pfami PF00787. PX. 1 hit.
    [Graphical view ]
    SMARTi SM00312. PX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64268. SSF64268. 2 hits.
    PROSITEi PS50195. PX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Cooperative binding of the cytoplasm to vacuole targeting pathway proteins, Cvt13 and Cvt20, to phosphatidylinositol 3-phosphate at the pre-autophagosomal structure is required for selective autophagy."
      Nice D.C. III, Sato T.K., Stromhaug P.E., Emr S.D., Klionsky D.J.
      J. Biol. Chem. 277:30198-30207(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG17 AND SNX4, MUTAGENESIS OF TYR-193.
    4. Cited for: NOMENCLATURE.
    5. "Retromer and the sorting nexins Snx4/41/42 mediate distinct retrieval pathways from yeast endosomes."
      Hettema E.H., Lewis M.J., Black M.W., Pelham H.R.B.
      EMBO J. 22:548-557(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SNX4.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway."
      Yorimitsu T., Klionsky D.J.
      Mol. Biol. Cell 16:1593-1605(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ATG11.
    9. Cited for: FUNCTION.
    10. "A yeast model system for functional analysis of the Niemann-Pick type C protein 1 homolog, Ncr1p."
      Berger A.C., Hanson P.K., Wylie Nichols J., Corbett A.H.
      Traffic 6:907-917(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response."
      Bernales S., McDonald K.L., Walter P.
      PLoS Biol. 4:E423-E423(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "A genomic screen for yeast mutants defective in selective mitochondria autophagy."
      Kanki T., Wang K., Baba M., Bartholomew C.R., Lynch-Day M.A., Du Z., Geng J., Mao K., Yang Z., Yen W.L., Klionsky D.J.
      Mol. Biol. Cell 20:4730-4738(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361 AND SER-363, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Membrane delivery to the yeast autophagosome from the Golgi-endosomal system."
      Ohashi Y., Munro S.
      Mol. Biol. Cell 21:3998-4008(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    16. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiATG20_YEAST
    AccessioniPrimary (citable) accession number: Q07528
    Secondary accession number(s): D6VRN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 358 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3