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Q07528

- ATG20_YEAST

UniProt

Q07528 - ATG20_YEAST

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Protein

Autophagy-related protein 20

Gene

ATG20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for cytoplasm to vacuole transport (Cvt), pexophagy and mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Functions in protein retrieval from the endocytic pathway. Required for proper sorting of the v-SNARE protein SNC1.7 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei192 – 1921Phosphatidylinositol 3-phosphateBy similarity
Binding sitei194 – 1941Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei218 – 2181Phosphatidylinositol 3-phosphateBy similarity
Binding sitei267 – 2671Phosphatidylinositol 3-phosphateBy similarity

GO - Molecular functioni

  1. phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  1. CVT pathway Source: SGD
  2. early endosome to Golgi transport Source: SGD
  3. macroautophagy Source: SGD
  4. mitochondrion degradation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29513-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 20
Alternative name(s):
Cytoplasm to vacuole targeting protein 20
Sorting nexin-42
Gene namesi
Name:ATG20
Synonyms:CVT20, SNX42
Ordered Locus Names:YDL113C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL113c.
SGDiS000002271. ATG20.

Subcellular locationi

GO - Cellular componenti

  1. endosome Source: SGD
  2. extrinsic component of membrane Source: SGD
  3. pre-autophagosomal structure Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi193 – 1931Y → A: Abolishes the intracellular punctate localization and decreases the cytoplasm to vacuole transport. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 640639Autophagy-related protein 20PRO_0000213824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei361 – 3611Phosphoserine1 Publication
Modified residuei363 – 3631Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ07528.
PaxDbiQ07528.
PeptideAtlasiQ07528.

Expressioni

Gene expression databases

GenevestigatoriQ07528.

Interactioni

Subunit structurei

Forms a complex with SNX4 and ATG17.

Binary interactionsi

WithEntry#Exp.IntActNotes
SNX4P470577EBI-36894,EBI-17610
YIP1P530392EBI-36894,EBI-25295

Protein-protein interaction databases

BioGridi31949. 49 interactions.
DIPiDIP-1593N.
IntActiQ07528. 10 interactions.
MINTiMINT-402385.
STRINGi4932.YDL113C.

Structurei

3D structure databases

ProteinModelPortaliQ07528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini140 – 301162PXPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili475 – 51238Sequence AnalysisAdd
BLAST
Coiled coili562 – 59332Sequence AnalysisAdd
BLAST

Domaini

The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization of ATG20 to the perivacuolar punctate structures.

Sequence similaritiesi

Belongs to the sorting nexin family.Curated
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5391.
GeneTreeiENSGT00530000066389.
HOGENOMiHOG000034139.
InParanoidiQ07528.
OMAiSARIHIL.
OrthoDBiEOG7M0P28.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
[Graphical view]
PfamiPF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 2 hits.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07528-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDLNDVQEN AKLNSETRNT GKAEPPHGTT EYVAEAEISK NGVGSPKKSP
60 70 80 90 100
KKGKVGKGDN NKVETELVHT ALLEKDNPFM EEGPTGFTKS ALLEIPGMRS
110 120 130 140 150
HNLKNPNEDY EDDSEGLLPL NQESNAETCR TSLSGSINSM NGETSASEEP
160 170 180 190 200
SVSNRKKSAR IHILEAKRVS EGQGRAYIAY VIQFENSTVQ RRYSDFESLR
210 220 230 240 250
SILIRLFPMT LIPPIPEKQS IKNYGKSITG SSSKYLLPSE GSGSVDLSLS
260 270 280 290 300
VIHASVNNSD EKLIRHRIRM LTEFLNKLLT NEEITKTSII TDFLDPNNHN
310 320 330 340 350
WHEFVNSSST FSSLPKSILQ CNPLDPTNTT RIHAMLPIPG SSSQLLLNKE
360 370 380 390 400
SNDKKMDKER SKSFTNIEQD YKQYENLLDN GIYKYNRRTT KTYHDLKSDY
410 420 430 440 450
NEIGEVFAQF AHEQAQVGEL AEQLSYLSNA FSGSSISLEK LVGRLYYNIN
460 470 480 490 500
EPLNESVHMA TSARELIKYR KLKYLQNEMI KKSLNSKRAQ LEKLEAQNNE
510 520 530 540 550
YKDVDKIIDN EMSKSHTINL ERPNNNTGSG GKSYGGKLFN GFNKLASMVK
560 570 580 590 600
DSVKYQETDP HTASINLKKE IEQLSESLEV TENDLEVISK VIKNDQLPKF
610 620 630 640
SKEREVDLSE ILKHYSRYMR NYARQNLEIW KEVKRHQDFA
Length:640
Mass (Da):72,546
Last modified:November 1, 1996 - v1
Checksum:i160977A8BECB6D93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74161 Genomic DNA. Translation: CAA98681.1.
BK006938 Genomic DNA. Translation: DAA11747.1.
PIRiS67656.
RefSeqiNP_010170.1. NM_001180172.1.

Genome annotation databases

EnsemblFungiiYDL113C; YDL113C; YDL113C.
GeneIDi851445.
KEGGisce:YDL113C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74161 Genomic DNA. Translation: CAA98681.1 .
BK006938 Genomic DNA. Translation: DAA11747.1 .
PIRi S67656.
RefSeqi NP_010170.1. NM_001180172.1.

3D structure databases

ProteinModelPortali Q07528.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31949. 49 interactions.
DIPi DIP-1593N.
IntActi Q07528. 10 interactions.
MINTi MINT-402385.
STRINGi 4932.YDL113C.

Proteomic databases

MaxQBi Q07528.
PaxDbi Q07528.
PeptideAtlasi Q07528.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL113C ; YDL113C ; YDL113C .
GeneIDi 851445.
KEGGi sce:YDL113C.

Organism-specific databases

CYGDi YDL113c.
SGDi S000002271. ATG20.

Phylogenomic databases

eggNOGi COG5391.
GeneTreei ENSGT00530000066389.
HOGENOMi HOG000034139.
InParanoidi Q07528.
OMAi SARIHIL.
OrthoDBi EOG7M0P28.

Enzyme and pathway databases

BioCyci YEAST:G3O-29513-MONOMER.

Miscellaneous databases

NextBioi 968693.

Gene expression databases

Genevestigatori Q07528.

Family and domain databases

Gene3Di 3.30.1520.10. 1 hit.
InterProi IPR001683. Phox.
[Graphical view ]
Pfami PF00787. PX. 1 hit.
[Graphical view ]
SMARTi SM00312. PX. 1 hit.
[Graphical view ]
SUPFAMi SSF64268. SSF64268. 2 hits.
PROSITEi PS50195. PX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Cooperative binding of the cytoplasm to vacuole targeting pathway proteins, Cvt13 and Cvt20, to phosphatidylinositol 3-phosphate at the pre-autophagosomal structure is required for selective autophagy."
    Nice D.C. III, Sato T.K., Stromhaug P.E., Emr S.D., Klionsky D.J.
    J. Biol. Chem. 277:30198-30207(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG17 AND SNX4, MUTAGENESIS OF TYR-193.
  4. Cited for: NOMENCLATURE.
  5. "Retromer and the sorting nexins Snx4/41/42 mediate distinct retrieval pathways from yeast endosomes."
    Hettema E.H., Lewis M.J., Black M.W., Pelham H.R.B.
    EMBO J. 22:548-557(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SNX4.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway."
    Yorimitsu T., Klionsky D.J.
    Mol. Biol. Cell 16:1593-1605(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ATG11.
  9. Cited for: FUNCTION.
  10. "A yeast model system for functional analysis of the Niemann-Pick type C protein 1 homolog, Ncr1p."
    Berger A.C., Hanson P.K., Wylie Nichols J., Corbett A.H.
    Traffic 6:907-917(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response."
    Bernales S., McDonald K.L., Walter P.
    PLoS Biol. 4:E423-E423(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "A genomic screen for yeast mutants defective in selective mitochondria autophagy."
    Kanki T., Wang K., Baba M., Bartholomew C.R., Lynch-Day M.A., Du Z., Geng J., Mao K., Yang Z., Yen W.L., Klionsky D.J.
    Mol. Biol. Cell 20:4730-4738(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361 AND SER-363, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Membrane delivery to the yeast autophagosome from the Golgi-endosomal system."
    Ohashi Y., Munro S.
    Mol. Biol. Cell 21:3998-4008(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiATG20_YEAST
AccessioniPrimary (citable) accession number: Q07528
Secondary accession number(s): D6VRN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3