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Protein

Autophagy-related protein 20

Gene

ATG20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for cytoplasm to vacuole transport (Cvt), pexophagy and mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Functions in protein retrieval from the endocytic pathway. Required for proper sorting of the v-SNARE protein SNC1.7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei194Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei218Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei267Phosphatidylinositol 3-phosphateBy similarity1

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  • CVT pathway Source: SGD
  • early endosome to Golgi transport Source: SGD
  • endocytosis Source: GO_Central
  • macroautophagy Source: SGD
  • mitophagy Source: SGD
  • vesicle organization Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29513-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 20
Alternative name(s):
Cytoplasm to vacuole targeting protein 20
Sorting nexin-42
Gene namesi
Name:ATG20
Synonyms:CVT20, SNX42
Ordered Locus Names:YDL113C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL113C.
SGDiS000002271. ATG20.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • endosome Source: SGD
  • endosome membrane Source: UniProtKB-SubCell
  • extrinsic component of membrane Source: SGD
  • pre-autophagosomal structure Source: SGD
  • pre-autophagosomal structure membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi193Y → A: Abolishes the intracellular punctate localization and decreases the cytoplasm to vacuole transport. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002138242 – 640Autophagy-related protein 20Add BLAST639

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei361PhosphoserineCombined sources1
Modified residuei363PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ07528.
PRIDEiQ07528.

PTM databases

iPTMnetiQ07528.

Interactioni

Subunit structurei

Forms a complex with SNX4 and ATG17.

Binary interactionsi

WithEntry#Exp.IntActNotes
SNX4P470577EBI-36894,EBI-17610
YIP1P530392EBI-36894,EBI-25295

Protein-protein interaction databases

BioGridi31949. 50 interactors.
DIPiDIP-1593N.
IntActiQ07528. 10 interactors.
MINTiMINT-402385.

Structurei

3D structure databases

ProteinModelPortaliQ07528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 301PXPROSITE-ProRule annotationAdd BLAST162

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili475 – 512Sequence analysisAdd BLAST38
Coiled coili562 – 593Sequence analysisAdd BLAST32

Domaini

The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization of ATG20 to the perivacuolar punctate structures.

Sequence similaritiesi

Belongs to the sorting nexin family.Curated
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000066389.
HOGENOMiHOG000034139.
InParanoidiQ07528.
OMAiSARIHIL.
OrthoDBiEOG092C1UWN.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
[Graphical view]
PfamiPF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 2 hits.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLNDVQEN AKLNSETRNT GKAEPPHGTT EYVAEAEISK NGVGSPKKSP
60 70 80 90 100
KKGKVGKGDN NKVETELVHT ALLEKDNPFM EEGPTGFTKS ALLEIPGMRS
110 120 130 140 150
HNLKNPNEDY EDDSEGLLPL NQESNAETCR TSLSGSINSM NGETSASEEP
160 170 180 190 200
SVSNRKKSAR IHILEAKRVS EGQGRAYIAY VIQFENSTVQ RRYSDFESLR
210 220 230 240 250
SILIRLFPMT LIPPIPEKQS IKNYGKSITG SSSKYLLPSE GSGSVDLSLS
260 270 280 290 300
VIHASVNNSD EKLIRHRIRM LTEFLNKLLT NEEITKTSII TDFLDPNNHN
310 320 330 340 350
WHEFVNSSST FSSLPKSILQ CNPLDPTNTT RIHAMLPIPG SSSQLLLNKE
360 370 380 390 400
SNDKKMDKER SKSFTNIEQD YKQYENLLDN GIYKYNRRTT KTYHDLKSDY
410 420 430 440 450
NEIGEVFAQF AHEQAQVGEL AEQLSYLSNA FSGSSISLEK LVGRLYYNIN
460 470 480 490 500
EPLNESVHMA TSARELIKYR KLKYLQNEMI KKSLNSKRAQ LEKLEAQNNE
510 520 530 540 550
YKDVDKIIDN EMSKSHTINL ERPNNNTGSG GKSYGGKLFN GFNKLASMVK
560 570 580 590 600
DSVKYQETDP HTASINLKKE IEQLSESLEV TENDLEVISK VIKNDQLPKF
610 620 630 640
SKEREVDLSE ILKHYSRYMR NYARQNLEIW KEVKRHQDFA
Length:640
Mass (Da):72,546
Last modified:November 1, 1996 - v1
Checksum:i160977A8BECB6D93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74161 Genomic DNA. Translation: CAA98681.1.
BK006938 Genomic DNA. Translation: DAA11747.1.
PIRiS67656.
RefSeqiNP_010170.1. NM_001180172.1.

Genome annotation databases

EnsemblFungiiYDL113C; YDL113C; YDL113C.
GeneIDi851445.
KEGGisce:YDL113C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74161 Genomic DNA. Translation: CAA98681.1.
BK006938 Genomic DNA. Translation: DAA11747.1.
PIRiS67656.
RefSeqiNP_010170.1. NM_001180172.1.

3D structure databases

ProteinModelPortaliQ07528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31949. 50 interactors.
DIPiDIP-1593N.
IntActiQ07528. 10 interactors.
MINTiMINT-402385.

PTM databases

iPTMnetiQ07528.

Proteomic databases

MaxQBiQ07528.
PRIDEiQ07528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL113C; YDL113C; YDL113C.
GeneIDi851445.
KEGGisce:YDL113C.

Organism-specific databases

EuPathDBiFungiDB:YDL113C.
SGDiS000002271. ATG20.

Phylogenomic databases

GeneTreeiENSGT00530000066389.
HOGENOMiHOG000034139.
InParanoidiQ07528.
OMAiSARIHIL.
OrthoDBiEOG092C1UWN.

Enzyme and pathway databases

BioCyciYEAST:G3O-29513-MONOMER.

Miscellaneous databases

PROiQ07528.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
[Graphical view]
PfamiPF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 2 hits.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATG20_YEAST
AccessioniPrimary (citable) accession number: Q07528
Secondary accession number(s): D6VRN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.