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Q07527

- TRM3_YEAST

UniProt

Q07527 - TRM3_YEAST

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Protein
tRNA (guanosine(18)-2'-O)-methyltransferase
Gene
TRM3, YDL112W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically methylates guanosine-18 in various tRNAs.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanosine(18) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(18) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1389 – 13891S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei1409 – 14091S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. tRNA (guanine) methyltransferase activity Source: SGD
  3. tRNA (guanosine-2'-O-)-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. tRNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:YDL112W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanosine(18)-2'-O)-methyltransferase (EC:2.1.1.34)
Alternative name(s):
tRNA [Gm18] methyltransferase
tRNA methylase 3
Gene namesi
Name:TRM3
Ordered Locus Names:YDL112W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL112w.
SGDiS000002270. TRM3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14361436tRNA (guanosine(18)-2'-O)-methyltransferase
PRO_0000270921Add
BLAST

Proteomic databases

MaxQBiQ07527.
PaxDbiQ07527.
PeptideAtlasiQ07527.

Expressioni

Gene expression databases

GenevestigatoriQ07527.

Interactioni

Protein-protein interaction databases

BioGridi31950. 52 interactions.
DIPiDIP-2598N.
IntActiQ07527. 5 interactions.
MINTiMINT-657104.

Structurei

3D structure databases

ProteinModelPortaliQ07527.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1013 – 10197Poly-Leu

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0566.
GeneTreeiENSGT00390000003939.
HOGENOMiHOG000141850.
KOiK15333.
OMAiVTADRWM.
OrthoDBiEOG7GFBDR.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
IPR025759. tRNA_mtfrase_TRM3.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF75217. SSF75217. 1 hit.

Sequencei

Sequence statusi: Complete.

Q07527-1 [UniParc]FASTAAdd to Basket

« Hide

MVGGALICKY LPREEQLKLI SDLIQNDSLE EVLELIETSP LDITTDSNIE     50
TPIFEKITEQ VIAYASIDGE AREMFRSSRA EMNKALRTSA QLLCCLPSVW 100
HKFQVWMSYR LNDIISENYK HLFNDNFGKK IVQPFFDSFA EEQNANIKHE 150
NLHLDILSLL HYLEVVYLFD ECKNGISSKC LDFIIVPLLG CNSEEIADSC 200
SKLMRWHIKY LSKCCNTDSN FDKLIWTFIK QLYAEGSQQA WKQKNSLSFL 250
LRFLLAAELS PELITYIKTD AYWRHIQTEL DNDVHEHRKL ALSILKLTIQ 300
KLSSHGITLQ TTFYKCNDLA NIEMLGSWKK FTTLYEMIAL DTSLNQIQAA 350
KQDIIKIFDN EHLHHSWGLI LLSTGLKSSM ESVRKYMMTL MFSITNMSAF 400
SSNLPLLTKT LLPAAMSAHY FDVKGVSCPH GEKLSLFVNN LLSQTTEGIS 450
DILFEILKLL VEKGTSFDPS RIYLSYGILV FFQNNKQKTI NSDHLSLIRK 500
LYDFAAEEEV LETTIQTIYL KFLLYIDPSV SASELLFTLV SHIKLKGGTY 550
KYVEPLFEDY RDLAVSHFDD LQAKENLTTN IGKDTIFDLL ASIIFDFKDI 600
DITPDFLIEV AKSKQDIPVY TSKAVTFLTQ LLSGEPSNGY TYENATALLS 650
YPNFTISTWK SINVNNLFKS VMEKFSLDKF KFFAEIYQKT YECRFDTIEL 700
NFNDLLSLYE MVKKSANQCS RESFKVKDSA YSSYFELLNT FLKTYALNRD 750
SSEGNDDELH ILLRLVDENI NKDNGNYLGN LAVCKLLYFI IDSYIHCSTS 800
VSDDDIFIVK FIFEKFSFIW ECINSERLVL KERDLHLMLI KGLFHPVILY 850
FGSNQYIDTL TSKLEEHAQT IISLSYSRRS LLPLLGSQLR VFMKFYGKLL 900
REDVNYWWLI NIIVGVFKQP QMDVNLYKLK PVISSLFDHK LNNYYIKGDE 950
LYEKVYGPDE ILARVSIIDS ILYANDQLKI RLIEKVTEKT NALYAIKRTD 1000
GAEALQRLLQ WQLLLLSLLT TNEKKLSETS MIRILKSIED ESSPLVRVYK 1050
EWFISSKVVD YYKTGNPKFA EDYLFSLLED HSKPVFVVSA EKICFMVLKD 1100
LRNDEKKYGF TQLLDRFICT LVPNAASNKP LVRHFSNSLI ISLWPTFKAY 1150
LSDHTLRNII ENLYSNAKKT QIFGQYRAGD ANIWDLKGDR KLTNMFGGVL 1200
KKVTDHDCPY ISESVFEKYL QEKDIVPIGT DERSLWLDKR DTNTESVNNA 1250
NISCDTSPLQ TKSGAWETVL DLDNKKSNDV VTRSELIVVS SLVDKPPNLG 1300
GICRLCDVLG VGLLTVQDIK VKNHPQFKNV AVTADRWMPM EEVALDEIAS 1350
FMKEKKKEGY TLIGLEQTDK SVKLDNNFQF PKKSLILLGT EAFGIPGTLL 1400
SELDLCLEIQ QFGVIRSMNI QTATAVIVHS YTVQHM 1436
Length:1,436
Mass (Da):165,048
Last modified:November 1, 1996 - v1
Checksum:i4B14823AF6E858F1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74160 Genomic DNA. Translation: CAA98680.1.
X95644 Genomic DNA. Translation: CAA64900.1.
Z74159 Genomic DNA. Translation: CAA98679.1.
BK006938 Genomic DNA. Translation: DAA11748.1.
PIRiS67655.
RefSeqiNP_010171.1. NM_001180171.1.

Genome annotation databases

EnsemblFungiiYDL112W; YDL112W; YDL112W.
GeneIDi851446.
KEGGisce:YDL112W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74160 Genomic DNA. Translation: CAA98680.1 .
X95644 Genomic DNA. Translation: CAA64900.1 .
Z74159 Genomic DNA. Translation: CAA98679.1 .
BK006938 Genomic DNA. Translation: DAA11748.1 .
PIRi S67655.
RefSeqi NP_010171.1. NM_001180171.1.

3D structure databases

ProteinModelPortali Q07527.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31950. 52 interactions.
DIPi DIP-2598N.
IntActi Q07527. 5 interactions.
MINTi MINT-657104.

Proteomic databases

MaxQBi Q07527.
PaxDbi Q07527.
PeptideAtlasi Q07527.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL112W ; YDL112W ; YDL112W .
GeneIDi 851446.
KEGGi sce:YDL112W.

Organism-specific databases

CYGDi YDL112w.
SGDi S000002270. TRM3.

Phylogenomic databases

eggNOGi COG0566.
GeneTreei ENSGT00390000003939.
HOGENOMi HOG000141850.
KOi K15333.
OMAi VTADRWM.
OrthoDBi EOG7GFBDR.

Enzyme and pathway databases

BioCyci YEAST:YDL112W-MONOMER.

Miscellaneous databases

NextBioi 968696.

Gene expression databases

Genevestigatori Q07527.

Family and domain databases

Gene3Di 3.40.1280.10. 1 hit.
InterProi IPR029028. Alpha/beta_knot_MTases.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
IPR025759. tRNA_mtfrase_TRM3.
[Graphical view ]
Pfami PF00588. SpoU_methylase. 1 hit.
[Graphical view ]
SUPFAMi SSF75217. SSF75217. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames."
    Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A.
    Yeast 12:1377-1384(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 955-1436.
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2'-O-ribose methyltransferase catalyzing the formation of Gm18 in tRNAs."
    Cavaille J., Chetouani F., Bachellerie J.-P.
    RNA 5:66-81(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRM3_YEAST
AccessioniPrimary (citable) accession number: Q07527
Secondary accession number(s): D6VRN8
, P89896, Q05336, Q7LGS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 861 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi