Q07527 (TRM3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanosine(18)-2'-O)-methyltransferase
EC=2.1.1.34

Alternative name(s):
tRNA [Gm18] methyltransferase
tRNA methylase 3

Gene names

Name:	TRM3
Ordered Locus Names:	YDL112W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1436 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically methylates guanosine-18 in various tRNAs. Ref.4
Catalytic activity	S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA.
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 861 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the RNA methyltransferase TrmH family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: SGD
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine) methyltransferase activity Inferred from direct assay Ref.4. Source: SGD tRNA (guanosine-2'-O-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1436	1436	tRNA (guanosine(18)-2'-O)-methyltransferase		PRO_0000270921
Regions
	Compositional bias	1013 – 1019	7	Poly-Leu
Sites
	Binding site	1389	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	1409	1	S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07527 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4B14823AF6E858F1	FASTA	1,436	165,048
	10 20 30 40 50 60 MVGGALICKY LPREEQLKLI SDLIQNDSLE EVLELIETSP LDITTDSNIE TPIFEKITEQ 70 80 90 100 110 120 VIAYASIDGE AREMFRSSRA EMNKALRTSA QLLCCLPSVW HKFQVWMSYR LNDIISENYK 130 140 150 160 170 180 HLFNDNFGKK IVQPFFDSFA EEQNANIKHE NLHLDILSLL HYLEVVYLFD ECKNGISSKC 190 200 210 220 230 240 LDFIIVPLLG CNSEEIADSC SKLMRWHIKY LSKCCNTDSN FDKLIWTFIK QLYAEGSQQA 250 260 270 280 290 300 WKQKNSLSFL LRFLLAAELS PELITYIKTD AYWRHIQTEL DNDVHEHRKL ALSILKLTIQ 310 320 330 340 350 360 KLSSHGITLQ TTFYKCNDLA NIEMLGSWKK FTTLYEMIAL DTSLNQIQAA KQDIIKIFDN 370 380 390 400 410 420 EHLHHSWGLI LLSTGLKSSM ESVRKYMMTL MFSITNMSAF SSNLPLLTKT LLPAAMSAHY 430 440 450 460 470 480 FDVKGVSCPH GEKLSLFVNN LLSQTTEGIS DILFEILKLL VEKGTSFDPS RIYLSYGILV 490 500 510 520 530 540 FFQNNKQKTI NSDHLSLIRK LYDFAAEEEV LETTIQTIYL KFLLYIDPSV SASELLFTLV 550 560 570 580 590 600 SHIKLKGGTY KYVEPLFEDY RDLAVSHFDD LQAKENLTTN IGKDTIFDLL ASIIFDFKDI 610 620 630 640 650 660 DITPDFLIEV AKSKQDIPVY TSKAVTFLTQ LLSGEPSNGY TYENATALLS YPNFTISTWK 670 680 690 700 710 720 SINVNNLFKS VMEKFSLDKF KFFAEIYQKT YECRFDTIEL NFNDLLSLYE MVKKSANQCS 730 740 750 760 770 780 RESFKVKDSA YSSYFELLNT FLKTYALNRD SSEGNDDELH ILLRLVDENI NKDNGNYLGN 790 800 810 820 830 840 LAVCKLLYFI IDSYIHCSTS VSDDDIFIVK FIFEKFSFIW ECINSERLVL KERDLHLMLI 850 860 870 880 890 900 KGLFHPVILY FGSNQYIDTL TSKLEEHAQT IISLSYSRRS LLPLLGSQLR VFMKFYGKLL 910 920 930 940 950 960 REDVNYWWLI NIIVGVFKQP QMDVNLYKLK PVISSLFDHK LNNYYIKGDE LYEKVYGPDE 970 980 990 1000 1010 1020 ILARVSIIDS ILYANDQLKI RLIEKVTEKT NALYAIKRTD GAEALQRLLQ WQLLLLSLLT 1030 1040 1050 1060 1070 1080 TNEKKLSETS MIRILKSIED ESSPLVRVYK EWFISSKVVD YYKTGNPKFA EDYLFSLLED 1090 1100 1110 1120 1130 1140 HSKPVFVVSA EKICFMVLKD LRNDEKKYGF TQLLDRFICT LVPNAASNKP LVRHFSNSLI 1150 1160 1170 1180 1190 1200 ISLWPTFKAY LSDHTLRNII ENLYSNAKKT QIFGQYRAGD ANIWDLKGDR KLTNMFGGVL 1210 1220 1230 1240 1250 1260 KKVTDHDCPY ISESVFEKYL QEKDIVPIGT DERSLWLDKR DTNTESVNNA NISCDTSPLQ 1270 1280 1290 1300 1310 1320 TKSGAWETVL DLDNKKSNDV VTRSELIVVS SLVDKPPNLG GICRLCDVLG VGLLTVQDIK 1330 1340 1350 1360 1370 1380 VKNHPQFKNV AVTADRWMPM EEVALDEIAS FMKEKKKEGY TLIGLEQTDK SVKLDNNFQF 1390 1400 1410 1420 1430 PKKSLILLGT EAFGIPGTLL SELDLCLEIQ QFGVIRSMNI QTATAVIVHS YTVQHM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames." Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A. Yeast 12:1377-1384(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 955-1436. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2'-O-ribose methyltransferase catalyzing the formation of Gm18 in tRNAs." Cavaille J., Chetouani F., Bachellerie J.-P. RNA 5:66-81(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74160 Genomic DNA. Translation: CAA98680.1. X95644 Genomic DNA. Translation: CAA64900.1. Z74159 Genomic DNA. Translation: CAA98679.1. BK006938 Genomic DNA. Translation: DAA11748.1.
PIR	S67655.
RefSeq	NP_010171.1. NM_001180171.1.
3D structure databases
ProteinModelPortal	Q07527.
SMR	Q07527. Positions 1284-1436.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2598N.
IntAct	Q07527. 7 interactions.
MINT	MINT-657104.
Proteomic databases
PaxDb	Q07527.
PeptideAtlas	Q07527.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDL112W; YDL112W; YDL112W.
GeneID	851446.
KEGG	sce:YDL112W.
Organism-specific databases
CYGD	YDL112w.
SGD	S000002270. TRM3.
Phylogenomic databases
eggNOG	COG0566.
GeneTree	ENSGT00390000003939.
HOGENOM	HOG000141850.
KO	K15333.
OMA	NIQTATA.
OrthoDB	EOG46HKJX.
Gene expression databases
Genevestigator	Q07527.
Family and domain databases
InterPro	IPR001537. SpoU_MeTrfase. IPR025759. tRNA_mtfrase_TRM3. [Graphical view]
Pfam	PF00588. SpoU_methylase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968696.

Entry information

Entry name

TRM3_YEAST

Accession

Primary (citable) accession number: Q07527
Secondary accession number(s): D6VRN8 , P89896, Q05336, Q7LGS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program