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Q07527

- TRM3_YEAST

UniProt

Q07527 - TRM3_YEAST

Protein

tRNA (guanosine(18)-2'-O)-methyltransferase

Gene

TRM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Specifically methylates guanosine-18 in various tRNAs.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + guanosine(18) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(18) in tRNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei1389 – 13891S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei1409 – 14091S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. RNA binding Source: InterPro
    2. tRNA (guanine) methyltransferase activity Source: SGD
    3. tRNA (guanosine-2'-O-)-methyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. tRNA methylation Source: SGD

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciYEAST:YDL112W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanosine(18)-2'-O)-methyltransferase (EC:2.1.1.34)
    Alternative name(s):
    tRNA [Gm18] methyltransferase
    tRNA methylase 3
    Gene namesi
    Name:TRM3
    Ordered Locus Names:YDL112W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL112w.
    SGDiS000002270. TRM3.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14361436tRNA (guanosine(18)-2'-O)-methyltransferasePRO_0000270921Add
    BLAST

    Proteomic databases

    MaxQBiQ07527.
    PaxDbiQ07527.
    PeptideAtlasiQ07527.

    Expressioni

    Gene expression databases

    GenevestigatoriQ07527.

    Interactioni

    Protein-protein interaction databases

    BioGridi31950. 52 interactions.
    DIPiDIP-2598N.
    IntActiQ07527. 5 interactions.
    MINTiMINT-657104.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07527.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1013 – 10197Poly-Leu

    Sequence similaritiesi

    Belongs to the RNA methyltransferase TrmH family.Curated

    Phylogenomic databases

    eggNOGiCOG0566.
    GeneTreeiENSGT00390000003939.
    HOGENOMiHOG000141850.
    KOiK15333.
    OMAiVTADRWM.
    OrthoDBiEOG7GFBDR.

    Family and domain databases

    Gene3Di3.40.1280.10. 1 hit.
    InterProiIPR029028. Alpha/beta_knot_MTases.
    IPR001537. SpoU_MeTrfase.
    IPR029026. tRNA_m1G_MTases_N.
    IPR025759. tRNA_mtfrase_TRM3.
    [Graphical view]
    PfamiPF00588. SpoU_methylase. 1 hit.
    [Graphical view]
    SUPFAMiSSF75217. SSF75217. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q07527-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVGGALICKY LPREEQLKLI SDLIQNDSLE EVLELIETSP LDITTDSNIE     50
    TPIFEKITEQ VIAYASIDGE AREMFRSSRA EMNKALRTSA QLLCCLPSVW 100
    HKFQVWMSYR LNDIISENYK HLFNDNFGKK IVQPFFDSFA EEQNANIKHE 150
    NLHLDILSLL HYLEVVYLFD ECKNGISSKC LDFIIVPLLG CNSEEIADSC 200
    SKLMRWHIKY LSKCCNTDSN FDKLIWTFIK QLYAEGSQQA WKQKNSLSFL 250
    LRFLLAAELS PELITYIKTD AYWRHIQTEL DNDVHEHRKL ALSILKLTIQ 300
    KLSSHGITLQ TTFYKCNDLA NIEMLGSWKK FTTLYEMIAL DTSLNQIQAA 350
    KQDIIKIFDN EHLHHSWGLI LLSTGLKSSM ESVRKYMMTL MFSITNMSAF 400
    SSNLPLLTKT LLPAAMSAHY FDVKGVSCPH GEKLSLFVNN LLSQTTEGIS 450
    DILFEILKLL VEKGTSFDPS RIYLSYGILV FFQNNKQKTI NSDHLSLIRK 500
    LYDFAAEEEV LETTIQTIYL KFLLYIDPSV SASELLFTLV SHIKLKGGTY 550
    KYVEPLFEDY RDLAVSHFDD LQAKENLTTN IGKDTIFDLL ASIIFDFKDI 600
    DITPDFLIEV AKSKQDIPVY TSKAVTFLTQ LLSGEPSNGY TYENATALLS 650
    YPNFTISTWK SINVNNLFKS VMEKFSLDKF KFFAEIYQKT YECRFDTIEL 700
    NFNDLLSLYE MVKKSANQCS RESFKVKDSA YSSYFELLNT FLKTYALNRD 750
    SSEGNDDELH ILLRLVDENI NKDNGNYLGN LAVCKLLYFI IDSYIHCSTS 800
    VSDDDIFIVK FIFEKFSFIW ECINSERLVL KERDLHLMLI KGLFHPVILY 850
    FGSNQYIDTL TSKLEEHAQT IISLSYSRRS LLPLLGSQLR VFMKFYGKLL 900
    REDVNYWWLI NIIVGVFKQP QMDVNLYKLK PVISSLFDHK LNNYYIKGDE 950
    LYEKVYGPDE ILARVSIIDS ILYANDQLKI RLIEKVTEKT NALYAIKRTD 1000
    GAEALQRLLQ WQLLLLSLLT TNEKKLSETS MIRILKSIED ESSPLVRVYK 1050
    EWFISSKVVD YYKTGNPKFA EDYLFSLLED HSKPVFVVSA EKICFMVLKD 1100
    LRNDEKKYGF TQLLDRFICT LVPNAASNKP LVRHFSNSLI ISLWPTFKAY 1150
    LSDHTLRNII ENLYSNAKKT QIFGQYRAGD ANIWDLKGDR KLTNMFGGVL 1200
    KKVTDHDCPY ISESVFEKYL QEKDIVPIGT DERSLWLDKR DTNTESVNNA 1250
    NISCDTSPLQ TKSGAWETVL DLDNKKSNDV VTRSELIVVS SLVDKPPNLG 1300
    GICRLCDVLG VGLLTVQDIK VKNHPQFKNV AVTADRWMPM EEVALDEIAS 1350
    FMKEKKKEGY TLIGLEQTDK SVKLDNNFQF PKKSLILLGT EAFGIPGTLL 1400
    SELDLCLEIQ QFGVIRSMNI QTATAVIVHS YTVQHM 1436
    Length:1,436
    Mass (Da):165,048
    Last modified:November 1, 1996 - v1
    Checksum:i4B14823AF6E858F1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74160 Genomic DNA. Translation: CAA98680.1.
    X95644 Genomic DNA. Translation: CAA64900.1.
    Z74159 Genomic DNA. Translation: CAA98679.1.
    BK006938 Genomic DNA. Translation: DAA11748.1.
    PIRiS67655.
    RefSeqiNP_010171.1. NM_001180171.1.

    Genome annotation databases

    EnsemblFungiiYDL112W; YDL112W; YDL112W.
    GeneIDi851446.
    KEGGisce:YDL112W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74160 Genomic DNA. Translation: CAA98680.1 .
    X95644 Genomic DNA. Translation: CAA64900.1 .
    Z74159 Genomic DNA. Translation: CAA98679.1 .
    BK006938 Genomic DNA. Translation: DAA11748.1 .
    PIRi S67655.
    RefSeqi NP_010171.1. NM_001180171.1.

    3D structure databases

    ProteinModelPortali Q07527.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31950. 52 interactions.
    DIPi DIP-2598N.
    IntActi Q07527. 5 interactions.
    MINTi MINT-657104.

    Proteomic databases

    MaxQBi Q07527.
    PaxDbi Q07527.
    PeptideAtlasi Q07527.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL112W ; YDL112W ; YDL112W .
    GeneIDi 851446.
    KEGGi sce:YDL112W.

    Organism-specific databases

    CYGDi YDL112w.
    SGDi S000002270. TRM3.

    Phylogenomic databases

    eggNOGi COG0566.
    GeneTreei ENSGT00390000003939.
    HOGENOMi HOG000141850.
    KOi K15333.
    OMAi VTADRWM.
    OrthoDBi EOG7GFBDR.

    Enzyme and pathway databases

    BioCyci YEAST:YDL112W-MONOMER.

    Miscellaneous databases

    NextBioi 968696.

    Gene expression databases

    Genevestigatori Q07527.

    Family and domain databases

    Gene3Di 3.40.1280.10. 1 hit.
    InterProi IPR029028. Alpha/beta_knot_MTases.
    IPR001537. SpoU_MeTrfase.
    IPR029026. tRNA_m1G_MTases_N.
    IPR025759. tRNA_mtfrase_TRM3.
    [Graphical view ]
    Pfami PF00588. SpoU_methylase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF75217. SSF75217. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames."
      Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A.
      Yeast 12:1377-1384(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 955-1436.
      Strain: ATCC 96604 / S288c / FY1679.
    4. "The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2'-O-ribose methyltransferase catalyzing the formation of Gm18 in tRNAs."
      Cavaille J., Chetouani F., Bachellerie J.-P.
      RNA 5:66-81(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTRM3_YEAST
    AccessioniPrimary (citable) accession number: Q07527
    Secondary accession number(s): D6VRN8
    , P89896, Q05336, Q7LGS3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 861 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3