Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanosine(18)-2'-O)-methyltransferase

Gene

TRM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanosine(18) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(18) in tRNA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1389 – 13891S-adenosyl-L-methionine; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

  • tRNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:YDL112W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanosine(18)-2'-O)-methyltransferase1 Publication (EC:2.1.1.341 Publication)
Alternative name(s):
tRNA [Gm18] ribose methylase1 Publication
tRNA methyltransferase 31 Publication
Gene namesi
Name:TRM31 Publication
Ordered Locus Names:YDL112WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDL112w.
EuPathDBiFungiDB:YDL112W.
SGDiS000002270. TRM3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14361436tRNA (guanosine(18)-2'-O)-methyltransferasePRO_0000270921Add
BLAST

Proteomic databases

MaxQBiQ07527.
PaxDbiQ07527.
PeptideAtlasiQ07527.

Interactioni

Protein-protein interaction databases

BioGridi31950. 52 interactions.
DIPiDIP-2598N.
IntActiQ07527. 5 interactions.
MINTiMINT-657104.
STRINGi4932.YDL112W.

Structurei

3D structure databases

ProteinModelPortaliQ07527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1365 – 13673S-adenosyl-L-methionine bindingBy similarity
Regioni1409 – 141810S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0566.
GeneTreeiENSGT00390000003939.
HOGENOMiHOG000141850.
KOiK15333.
OMAiVTADRWM.
OrthoDBiEOG7GFBDR.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
IPR025759. tRNA_mtfrase_TRM3.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF75217. SSF75217. 1 hit.
PROSITEiPS51623. SAM_MT_TRMH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGGALICKY LPREEQLKLI SDLIQNDSLE EVLELIETSP LDITTDSNIE
60 70 80 90 100
TPIFEKITEQ VIAYASIDGE AREMFRSSRA EMNKALRTSA QLLCCLPSVW
110 120 130 140 150
HKFQVWMSYR LNDIISENYK HLFNDNFGKK IVQPFFDSFA EEQNANIKHE
160 170 180 190 200
NLHLDILSLL HYLEVVYLFD ECKNGISSKC LDFIIVPLLG CNSEEIADSC
210 220 230 240 250
SKLMRWHIKY LSKCCNTDSN FDKLIWTFIK QLYAEGSQQA WKQKNSLSFL
260 270 280 290 300
LRFLLAAELS PELITYIKTD AYWRHIQTEL DNDVHEHRKL ALSILKLTIQ
310 320 330 340 350
KLSSHGITLQ TTFYKCNDLA NIEMLGSWKK FTTLYEMIAL DTSLNQIQAA
360 370 380 390 400
KQDIIKIFDN EHLHHSWGLI LLSTGLKSSM ESVRKYMMTL MFSITNMSAF
410 420 430 440 450
SSNLPLLTKT LLPAAMSAHY FDVKGVSCPH GEKLSLFVNN LLSQTTEGIS
460 470 480 490 500
DILFEILKLL VEKGTSFDPS RIYLSYGILV FFQNNKQKTI NSDHLSLIRK
510 520 530 540 550
LYDFAAEEEV LETTIQTIYL KFLLYIDPSV SASELLFTLV SHIKLKGGTY
560 570 580 590 600
KYVEPLFEDY RDLAVSHFDD LQAKENLTTN IGKDTIFDLL ASIIFDFKDI
610 620 630 640 650
DITPDFLIEV AKSKQDIPVY TSKAVTFLTQ LLSGEPSNGY TYENATALLS
660 670 680 690 700
YPNFTISTWK SINVNNLFKS VMEKFSLDKF KFFAEIYQKT YECRFDTIEL
710 720 730 740 750
NFNDLLSLYE MVKKSANQCS RESFKVKDSA YSSYFELLNT FLKTYALNRD
760 770 780 790 800
SSEGNDDELH ILLRLVDENI NKDNGNYLGN LAVCKLLYFI IDSYIHCSTS
810 820 830 840 850
VSDDDIFIVK FIFEKFSFIW ECINSERLVL KERDLHLMLI KGLFHPVILY
860 870 880 890 900
FGSNQYIDTL TSKLEEHAQT IISLSYSRRS LLPLLGSQLR VFMKFYGKLL
910 920 930 940 950
REDVNYWWLI NIIVGVFKQP QMDVNLYKLK PVISSLFDHK LNNYYIKGDE
960 970 980 990 1000
LYEKVYGPDE ILARVSIIDS ILYANDQLKI RLIEKVTEKT NALYAIKRTD
1010 1020 1030 1040 1050
GAEALQRLLQ WQLLLLSLLT TNEKKLSETS MIRILKSIED ESSPLVRVYK
1060 1070 1080 1090 1100
EWFISSKVVD YYKTGNPKFA EDYLFSLLED HSKPVFVVSA EKICFMVLKD
1110 1120 1130 1140 1150
LRNDEKKYGF TQLLDRFICT LVPNAASNKP LVRHFSNSLI ISLWPTFKAY
1160 1170 1180 1190 1200
LSDHTLRNII ENLYSNAKKT QIFGQYRAGD ANIWDLKGDR KLTNMFGGVL
1210 1220 1230 1240 1250
KKVTDHDCPY ISESVFEKYL QEKDIVPIGT DERSLWLDKR DTNTESVNNA
1260 1270 1280 1290 1300
NISCDTSPLQ TKSGAWETVL DLDNKKSNDV VTRSELIVVS SLVDKPPNLG
1310 1320 1330 1340 1350
GICRLCDVLG VGLLTVQDIK VKNHPQFKNV AVTADRWMPM EEVALDEIAS
1360 1370 1380 1390 1400
FMKEKKKEGY TLIGLEQTDK SVKLDNNFQF PKKSLILLGT EAFGIPGTLL
1410 1420 1430
SELDLCLEIQ QFGVIRSMNI QTATAVIVHS YTVQHM
Length:1,436
Mass (Da):165,048
Last modified:November 1, 1996 - v1
Checksum:i4B14823AF6E858F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74160 Genomic DNA. Translation: CAA98680.1.
X95644 Genomic DNA. Translation: CAA64900.1.
Z74159 Genomic DNA. Translation: CAA98679.1.
BK006938 Genomic DNA. Translation: DAA11748.1.
PIRiS67655.
RefSeqiNP_010171.1. NM_001180171.1.

Genome annotation databases

EnsemblFungiiYDL112W; YDL112W; YDL112W.
GeneIDi851446.
KEGGisce:YDL112W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74160 Genomic DNA. Translation: CAA98680.1.
X95644 Genomic DNA. Translation: CAA64900.1.
Z74159 Genomic DNA. Translation: CAA98679.1.
BK006938 Genomic DNA. Translation: DAA11748.1.
PIRiS67655.
RefSeqiNP_010171.1. NM_001180171.1.

3D structure databases

ProteinModelPortaliQ07527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31950. 52 interactions.
DIPiDIP-2598N.
IntActiQ07527. 5 interactions.
MINTiMINT-657104.
STRINGi4932.YDL112W.

Proteomic databases

MaxQBiQ07527.
PaxDbiQ07527.
PeptideAtlasiQ07527.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL112W; YDL112W; YDL112W.
GeneIDi851446.
KEGGisce:YDL112W.

Organism-specific databases

CYGDiYDL112w.
EuPathDBiFungiDB:YDL112W.
SGDiS000002270. TRM3.

Phylogenomic databases

eggNOGiCOG0566.
GeneTreeiENSGT00390000003939.
HOGENOMiHOG000141850.
KOiK15333.
OMAiVTADRWM.
OrthoDBiEOG7GFBDR.

Enzyme and pathway databases

BioCyciYEAST:YDL112W-MONOMER.

Miscellaneous databases

NextBioi968696.
PROiQ07527.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
IPR025759. tRNA_mtfrase_TRM3.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF75217. SSF75217. 1 hit.
PROSITEiPS51623. SAM_MT_TRMH_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames."
    Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A.
    Yeast 12:1377-1384(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 955-1436.
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2'-O-ribose methyltransferase catalyzing the formation of Gm18 in tRNAs."
    Cavaille J., Chetouani F., Bachellerie J.-P.
    RNA 5:66-81(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRM3_YEAST
AccessioniPrimary (citable) accession number: Q07527
Secondary accession number(s): D6VRN8
, P89896, Q05336, Q7LGS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 861 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.