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Q07524

- Q07524_TROMA

UniProt

Q07524 - Q07524_TROMA

Protein

Xyloglucan endotransglucosylase/hydrolase

Gene
N/A
Organism
Tropaeolum majus (Common nasturtium) (Indian cress)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.UniRule annotation

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.UniRule annotation

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseUniRule annotationImported, Hydrolase, TransferaseUniRule annotation

    Keywords - Biological processi

    Cell wall biogenesis/degradationUniRule annotation

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolaseUniRule annotation (EC:2.4.1.207UniRule annotation)
    OrganismiTropaeolum majus (Common nasturtium) (Indian cress)Imported
    Taxonomic identifieri4020 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesTropaeolaceaeTropaeolum

    Subcellular locationi

    Secretedcell wall UniRule annotation. Secretedextracellular spaceapoplast UniRule annotation

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    ApoplastUniRule annotation, Cell wallUniRule annotation, Secreted

    PTM / Processingi

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.UniRule annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2UWAX-ray1.80A/B/C25-295[»]
    2UWBX-ray2.00A/B25-295[»]
    2UWCX-ray2.30A/B25-295[»]
    2VH9X-ray2.10A/B1-295[»]
    ProteinModelPortaliQ07524.
    SMRiQ07524. Positions 25-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ07524.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.UniRule annotation

    Keywords - Domaini

    SignalUniRule annotation

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q07524-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPNILSIFL HLLPILMFSS SCLGQGPPSP GYYPSSQITS LGFDQGYTNL    50
    WGPQHQRVDQ GSLTIWLDST SGSGFKSINR YRSGYFGANI KLQSGYTAGV 100
    ITSFYLSNNQ DYPGKHDEID IEFLGTIPGK PYTLQTNVFI EGSGDYNIIG 150
    REMRIHLWFD PTQDYHNYAI YWTPSEIIFF VDDVPIRRYP RKSDATFPLR 200
    PLWVYGSVWD ASSWATENGK YKADYRYQPF VGKYEDFKLG SCTVEAASSC 250
    NPASVSPYGQ LSQQQVAAME WVQKNYMVYN YCDDPTRDHT LTPEC 295
    Length:295
    Mass (Da):33,516
    Last modified:November 1, 1996 - v1
    Checksum:i789A901E63D18CEB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68254 mRNA. Translation: CAA48324.1.
    PIRiS48102.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68254 mRNA. Translation: CAA48324.1 .
    PIRi S48102.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2UWA X-ray 1.80 A/B/C 25-295 [» ]
    2UWB X-ray 2.00 A/B 25-295 [» ]
    2UWC X-ray 2.30 A/B 25-295 [» ]
    2VH9 X-ray 2.10 A/B 1-295 [» ]
    ProteinModelPortali Q07524.
    SMRi Q07524. Positions 25-295.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q07524.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterisation of a xyloglucan specific endo 1,4 beta D glucanase (xyloglucan endo-transglycosylase) from nasturtium seeds."
      De Silva J., Jarman C.D., Arrowsmith D.A., Stronach M.S., Sidebottom C., Reid J.S.G.
      Plant J. 3:701-711(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: CotyledonImported.
    2. "Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism."
      Baumann M.J., Eklof J.M., Michel G., Kallas A.M., Teeri T.T., Czjzek M., Brumer H.
      Plant Cell 19:1947-1963(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 25-295.
    3. "Analysis of nasturtium TmNXG1 complexes by crystallography and molecular dynamics provides detailed insight into substrate recognition by family GH16 xyloglucan endo-transglycosylases and endo-hydrolases."
      Mark P., Baumann M.J., Eklof J.M., Gullfot F., Michel G., Kallas A.M., Teeri T.T., Brumer H., Czjzek M.
      Proteins 75:820-836(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS).

    Entry informationi

    Entry nameiQ07524_TROMA
    AccessioniPrimary (citable) accession number: Q07524
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 1, 1996
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3