Q07512 (FLS_PETHY) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 70.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Flavonol synthase/flavanone 3-hydroxylase Short name=FLS EC=1.14.11.23 EC=1.14.11.9 | ||
| Gene names |
| ||
| Organism | Petunia hybrida (Petunia) | ||
| Taxonomic identifier | 4102 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia![]() |
Protein attributes
| Sequence length | 348 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin. |
| Catalytic activity | A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O. A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2. |
| Cofactor | Binds 1 ascorbate molecule per subunit. Binds 1 iron ion per subunit. |
| Pathway | |
| Subcellular location | |
| Developmental stage | Expressed at highest level during the first stage of flower development. |
| Sequence similarities | Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Flavonoid biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | Iron Metal-binding Vitamin C |
| Molecular function | Dioxygenase Oxidoreductase |
| Gene Ontology (GO) | |
| Biological_process | flavonol biosynthetic process Inferred from electronic annotation. Source: GOC |
| Cellular_component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW flavonol synthase activityInferred from electronic annotation. Source: EC iron ion bindingInferred from electronic annotation. Source: InterPro naringenin 3-dioxygenase activityInferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygenInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 348 | 348 | Flavonol synthase/flavanone 3-hydroxylase | PRO_0000067296 | |||||
Regions | |||||||||
| Domain | 209 – 309 | 101 | Fe2OG dioxygenase | ||||||
Sites | |||||||||
| Metal binding | 234 | 1 | Iron By similarity | ||||||
| Metal binding | 236 | 1 | Iron By similarity | ||||||
| Metal binding | 290 | 1 | Iron By similarity | ||||||
Sequences
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References
| [1] | "Cloning and expression of flavonol synthase from Petunia hybrida." Holton T.A., Brugliera F., Tanaka Y. Plant J. 4:1003-1010(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Old Glory Blue. Tissue: Petal. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z22543 mRNA. Translation: CAA80264.1. |
| PIR | S33510. |
3D structure databases | |
| ProteinModelPortal | Q07512. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Enzyme and pathway databases | |
| UniPathway | UPA00154. |
Family and domain databases | |
| InterPro | IPR026992. DIOX_N. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglu/Fe-dep_dioxygenase. [Graphical view] |
| Pfam | PF03171. 2OG-FeII_Oxy. 1 hit. PF14226. DIOX_N. 1 hit. [Graphical view] |
| PRINTS | PR00682. IPNSYNTHASE. |
| PROSITE | PS51471. FE2OG_OXY. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FLS_PETHY | ||||||||
| Accession | Primary (citable) accession number: Q07512 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
