Q07512 (FLS_PETHY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flavonol synthase/flavanone 3-hydroxylase
Short name=FLS
EC=1.14.11.23
EC=1.14.11.9

Gene names

Name:

FL

Organism

Petunia hybrida (Petunia)

Taxonomic identifier

4102 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin.
Catalytic activity	A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O. A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.
Cofactor	Binds 1 ascorbate molecule per subunit. Binds 1 iron ion per subunit.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Subcellular location	Cytoplasm.
Developmental stage	Expressed at highest level during the first stage of flower development.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological_process	flavonol biosynthetic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW flavonol synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Flavonol synthase/flavanone 3-hydroxylase		PRO_0000067296
Regions
	Domain	209 – 309	101	Fe2OG dioxygenase
Sites
	Metal binding	234	1	Iron By similarity
	Metal binding	236	1	Iron By similarity
	Metal binding	290	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07512 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B39E1E4381DE6379	FASTA	348	39,427
	10 20 30 40 50 60 MKTAQGVSAT LTMEVARVQA IASLSKCMDT IPSEYIRSEN EQPAATTLHG VVLQVPVIDL 70 80 90 100 110 120 RDPDENKMVK LIADASKEWG IFQLINHGIP DEAIADLQKV GKEFFEHVPQ EEKELIAKTP 130 140 150 160 170 180 GSNDIEGYGT SLQKEVEGKK GWVDHLFHKI WPPSAVNYRY WPKNPPSYRE ANEEYGKRMR 190 200 210 220 230 240 EVVDRIFKSL SLGLGLEGHE MIEAAGGDEI VYLLKINYYP PCPRPDLALG VVAHTDMSYI 250 260 270 280 290 300 TILVPNEVQG LQVFKDGHWY DVKYIPNALI VHIGDQVEIL SNGKYKSVYH RTTVNKDKTR 310 320 330 340 MSWPVFLEPP SEHEVGPIPK LLSEANPPKF KTKKYKDYVY CKLNKLPQ

References

[1]	"Cloning and expression of flavonol synthase from Petunia hybrida." Holton T.A., Brugliera F., Tanaka Y. Plant J. 4:1003-1010(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Old Glory Blue. Tissue: Petal.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z22543 mRNA. Translation: CAA80264.1.
PIR	S33510.
3D structure databases
ProteinModelPortal	Q07512.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00154.
Family and domain databases
InterPro	IPR026992. DIOX_N. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglu/Fe-dep_dioxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. PF14226. DIOX_N. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLS_PETHY

Accession

Primary (citable) accession number: Q07512

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program