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Protein

Formate dehydrogenase, mitochondrial

Gene

FDH1

Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in formate-dependent oxygen uptake coupled to ATP synthesis.

Catalytic activityi

Formate + NAD+ = CO2 + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei258 – 2581NAD; via carbonyl oxygenBy similarity
Active sitei287 – 2871By similarity
Binding sitei311 – 3111NADBy similarity
Active sitei335 – 3351Proton donorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi204 – 2052NADBy similarity
Nucleotide bindingi285 – 2873NADBy similarity
Nucleotide bindingi335 – 3384NADBy similarity

GO - Molecular functioni

  1. formate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  2. NAD binding Source: InterPro
  3. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase, mitochondrial (EC:1.2.1.2)
Alternative name(s):
NAD-dependent formate dehydrogenase
Short name:
FDH
Gene namesi
Name:FDH1
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2525Mitochondrion1 PublicationAdd
BLAST
Chaini26 – 381356Formate dehydrogenase, mitochondrialPRO_0000007196Add
BLAST

Expressioni

Tissue specificityi

Found at high levels in developing tubers, at intermediate level in stems, veins, stolons, and stamens, and at low level in leaves and roots.

Inductioni

Induced very rapidly by wounding, and slower by darkness, chilling, drought, hypoxia, and treatments with formate, abscisic acid, serine, sarcosine, pyruvate, acetate, ethanol or methanol.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiQ07511. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ07511.
SMRiQ07511. Positions 33-376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiQ07511.
KOiK00122.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07511-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMSRVASTA ARAITSPSSL VFTRELQASP GPKKIVGVFY KANEYAEMNP
60 70 80 90 100
NFLGCAENAL GIREWLESKG HQYIVTPDKE GPDCELEKHI PDLHVLISTP
110 120 130 140 150
FHPAYVTAER IKKAKNLQLL LTAGIGSDHV DLKAAAAAGL TVAEVTGSNT
160 170 180 190 200
VSVAEDELMR ILILVRNFLP GHHQVINGEW NVAAIAHRAY DLEGKTVGTV
210 220 230 240 250
GAGRIGRLLL QRLKPFNCNL LYHDRLKMDS ELENQIGAKF EEDLDKMLSK
260 270 280 290 300
CDIVVINTPL TEKTKGMFDK ERIAKLKKGV LIVNNARGAI MDTQAVVDAC
310 320 330 340 350
NSGHIAGYSG DVWYPQPAPK DHPWRYMPNQ AMTPHISGTT IDAQLRYAAG
360 370 380
TKDMLDRYFK GEDFPAENYI VKDGELAPQY R
Length:381
Mass (Da):42,038
Last modified:December 6, 2002 - v2
Checksum:i920D351AC5A3A00E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99991 mRNA. Translation: CAB17080.1.
Z99992 Genomic DNA. No translation available.
Z21493 mRNA. Translation: CAA79702.2.
PIRiJQ2272.
RefSeqiNP_001274827.1. NM_001287898.1.
UniGeneiStu.477.

Genome annotation databases

GeneIDi102577429.
KEGGisot:102577429.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99991 mRNA. Translation: CAB17080.1.
Z99992 Genomic DNA. No translation available.
Z21493 mRNA. Translation: CAA79702.2.
PIRiJQ2272.
RefSeqiNP_001274827.1. NM_001287898.1.
UniGeneiStu.477.

3D structure databases

ProteinModelPortaliQ07511.
SMRiQ07511. Positions 33-376.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ07511. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi102577429.
KEGGisot:102577429.

Phylogenomic databases

InParanoidiQ07511.
KOiK00122.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evidence for multiple copies of formate dehydrogenase genes in plants: isolation of three potato fdh genes fdh1, fdh2 and fdh3."
    Hourton-Cabassa C., Ambard-Bretteville F., Remy R., Colas des Francs-Small C.
    Plant Gene Register PGR98-102
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. BF15.
  2. "Identification of a major soluble protein in mitochondria from nonphotosynthetic tissues as NAD-dependent formate dehydrogenase."
    Colas des Francs-Small C., Ambard-Bretteville F., Small I.D., Remy R.
    Plant Physiol. 102:1171-1177(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-381, PARTIAL PROTEIN SEQUENCE.
    Strain: cv. BF15.
    Tissue: Tuber.
  3. Colas des Francs-Small C.C.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
    Strain: cv. BF15.
    Tissue: Tuber.
  4. "Variation of the polypeptide composition of mitochondria isolated from different potato tissues."
    Colas des Francs-Small C., Ambard-Bretteville F., Darpas A., Sallantin M., Huet J.-C., Pernollet J.-C., Remy R.
    Plant Physiol. 98:273-278(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-54.
    Strain: cv. BF15.
    Tissue: Tuber.
  5. Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiFDH_SOLTU
AccessioniPrimary (citable) accession number: Q07511
Secondary accession number(s): Q9ZR28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 6, 2002
Last modified: January 7, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

There are two other putative pseudogenes, FDH2 and FDH3.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.