Reviewed, UniProtKB/Swiss-Prot Q07511 (FDH_SOLTU)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase, mitochondrial
    EC=1.2.1.2
Alternative name(s):
    NAD-dependent formate dehydrogenase
      Short name=FDH

Gene names

Name:

FDH1

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in formate-dependent oxygen uptake coupled to ATP synthesis.
Catalytic activity	Formate + NAD+ = CO2 + NADH.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion.
Tissue specificity	Found at high levels in developing tubers, at intermediate level in stems, veins, stolons, and stamens, and at low level in leaves and roots.
Induction	Induced very rapidly by wounding, and slower by darkness, chilling, drought, hypoxia, and treatments with formate, abscisic acid, serine, sarcosine, pyruvate, acetate, ethanol or methanol.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.
Caution	There are two other putative pseudogenes, FDH2 and FDH3.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 25	25	Mitochondrion Ref.4
	Chain	26 – 381	356	Formate dehydrogenase, mitochondrial		PRO_0000007196
Regions
	Nucleotide binding	204 – 205	2	NAD By similarity
	Nucleotide binding	285 – 287	3	NAD By similarity
	Nucleotide binding	335 – 338	4	NAD By similarity
Sites
	Active site	287	1	By similarity
	Active site	335	1	Proton donor By similarity
	Binding site	258	1	NAD; via carbonyl oxygen By similarity
	Binding site	311	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07511-1 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: 920D351AC5A3A00E	FASTA	381	42,038
	10 20 30 40 50 60 MAMSRVASTA ARAITSPSSL VFTRELQASP GPKKIVGVFY KANEYAEMNP NFLGCAENAL 70 80 90 100 110 120 GIREWLESKG HQYIVTPDKE GPDCELEKHI PDLHVLISTP FHPAYVTAER IKKAKNLQLL 130 140 150 160 170 180 LTAGIGSDHV DLKAAAAAGL TVAEVTGSNT VSVAEDELMR ILILVRNFLP GHHQVINGEW 190 200 210 220 230 240 NVAAIAHRAY DLEGKTVGTV GAGRIGRLLL QRLKPFNCNL LYHDRLKMDS ELENQIGAKF 250 260 270 280 290 300 EEDLDKMLSK CDIVVINTPL TEKTKGMFDK ERIAKLKKGV LIVNNARGAI MDTQAVVDAC 310 320 330 340 350 360 NSGHIAGYSG DVWYPQPAPK DHPWRYMPNQ AMTPHISGTT IDAQLRYAAG TKDMLDRYFK 370 380 GEDFPAENYI VKDGELAPQY R

References

[1]	"Evidence for multiple copies of formate dehydrogenase genes in plants: isolation of three potato fdh genes fdh1, fdh2 and fdh3." Hourton-Cabassa C., Ambard-Bretteville F., Remy R., Colas des Francs-Small C. Plant Gene Register PGR98-102 Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. BF15.
[2]	"Identification of a major soluble protein in mitochondria from nonphotosynthetic tissues as NAD-dependent formate dehydrogenase." Colas des Francs-Small C., Ambard-Bretteville F., Small I.D., Remy R. Plant Physiol. 102:1171-1177(1993) [PubMed: 8278546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-381, PARTIAL PROTEIN SEQUENCE. Strain: cv. BF15. Tissue: Tuber.
[3]	Colas des Francs-Small C.C. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS. Strain: cv. BF15. Tissue: Tuber.
[4]	"Variation of the polypeptide composition of mitochondria isolated from different potato tissues." Colas des Francs-Small C., Ambard-Bretteville F., Darpas A., Sallantin M., Huet J.-C., Pernollet J.-C., Remy R. Plant Physiol. 98:273-278(1992) [PubMed: 16668624] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-54. Strain: cv. BF15. Tissue: Tuber.
[5]	"Stress induction of mitochondrial formate dehydrogenase in potato leaves." Hourton-Cabassa C., Ambard-Bretteville F., Moreau F., Davy de Virville J., Remy R., Colas des Francs-Small C. Plant Physiol. 116:627-635(1998) [PubMed: 9490763] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z99991 mRNA. Translation: CAB17080.1. Z99992 Genomic DNA. No translation available. Z21493 mRNA. Translation: CAA79702.2.
PIR	JQ2272.
3D structure databases
SMR	Q07511. Positions 33-376.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q07511. 1 interaction.
Enzyme and pathway databases
BRENDA	1.2.1.2. 296.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDH_SOLTU

Accession

Primary (citable) accession number: Q07511
Secondary accession number(s): Q9ZR28

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 6, 2002
Last modified:	January 19, 2010
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)