Reviewed, UniProtKB/Swiss-Prot Q07498 (EPHB3_CHICK)

Names and origin

Protein names

Recommended name:
    Ephrin type-B receptor 3
    EC=2.7.10.1
Alternative name(s):
    EPH-like kinase 10
      Short name=EK10
      Short name=cEK10

Gene names

Name:	EPHB3
Synonyms:	CEK10

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	988 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-B family.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Present in 10-day embryonic brain and body tissues. Prominent expression in kidney. Lower expression in lung, and barely detectable in brain, liver, heart, skeletal muscle and thymus.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to plasma membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q07498-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q07498-2)

The sequence of this isoform differs from the canonical sequence as follows:
     558-572: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 988	›988	Ephrin type-B receptor 3		PRO_0000160276
Regions
	Topological domain	‹1 – 534	›534	Extracellular Potential
	Transmembrane	535 – 555	21	Potential
	Topological domain	556 – 988	433	Cytoplasmic Potential
	Domain	312 – 416	105	Fibronectin type-III 1
	Domain	426 – 518	93	Fibronectin type-III 2
	Domain	623 – 886	264	Protein kinase
	Domain	915 – 979	65	SAM
	Nucleotide binding	629 – 637	9	ATP By similarity
	Motif	986 – 988	3	PDZ-binding Potential
	Compositional bias	171 – 308	138	Cys-rich
Sites
	Active site	748	1	Proton acceptor By similarity
	Binding site	655	1	ATP By similarity
Amino acid modifications
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Glycosylation	418	1	N-linked (GlcNAc...) Potential
	Disulfide bond	53 ↔ 88		By similarity
Natural variations
	Alternative sequence	558 – 572	15	Missing in isoform Short.		VSP_003019
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EEA0D39C03FFD3C8	FASTA	988	109,579
	10 20 30 40 50 60 GVSSRARRPP GSSRSSRRGV TSELAWTTHP ETGWEEVSGY DEAMNPIRTY QVCNVREANQ 70 80 90 100 110 120 NNWLRTKFIQ RQDVQRVYVE LKFTVRDCNS IPNIPGSCKE TFNLFYYESD TDSASANSPF 130 140 150 160 170 180 WMENPYIKVD TIAPDESFSK LESGRVNTKV RSFGPLSKNG FYLAFQDLGA CMSLISVRAF 190 200 210 220 230 240 YKKCSNTIAG FAIFPETLTG AEPTSLVIAP GTCIPNAVEV SVPLKLYCNG DGEWMVPVGA 250 260 270 280 290 300 CTCAAGYEPA MKDTQCQACG PGTFKSKQGE GPCSPCPPNS RTTAGAATVC ICRSGFFRAD 310 320 330 340 350 360 ADPADSACTS VPSAPRSVIS NVNETSLVLE WSEPQDAGGR DDLLYNVICK KCSVERRLCS 370 380 390 400 410 420 RCDDNVEFVP RQLGLTGLTE RRIYISKVMA HPQYTFEIQA VNGISSKSPY PPHFASVNIT 430 440 450 460 470 480 TNQAAPSAVP TMHLHSSTGN SMTLSWTPPE RPNGIILDYE IKYSEKQGQG DGIANTVTSQ 490 500 510 520 530 540 KNSVRLDGLK ANARYMVQVR ARTVAGYGRY SLPTEFQTTA EDGSTSKTFQ ELPLIVGSAT 550 560 570 580 590 600 AGLLFVIVVV IIAIVCFRKG MVTEQLLSSP LGRKQRNSTD PEYTEKLQQY VTPGMKVYID 610 620 630 640 650 660 PFTYEDPNEA VREFAKEIDI SCVKIEEVIG AGEFGEVCRG RLKLPGRREI FVAIKTLKVG 670 680 690 700 710 720 YTERQRRDFL SEASIMGQFD HPNIIHLEGV VTKSRPVMII TEFMENCALD SFLRLNDGQF 730 740 750 760 770 780 TVIQLVGMLR GIAAGMKYLS EMNYVHRDLA ARNILVNSNL VCKVSDFGLS RFLEDDPADP 790 800 810 820 830 840 TYTSSLGGKI PIRWTAPEAI AYRKFTSASD VWSYGIVMWE VMSYGERPYW DMSNQDVINA 850 860 870 880 890 900 VEQDYRLPPP MDCPTALHQL MLDCWVRDRN LRPKFAQIVN TLDKLIRNAA SLKVIASVQS 910 920 930 940 950 960 GVSQPLLDRT VPDYTTFTTV GDWLDAIKMG RYKENFVNAG FASFDLVAQM TAEDLLRIGV 970 980 TLAGHQKKIL SSIQDMRLQM NQTLPVQV
	Isoform Short. Checksum: 533459035D7F9200	FASTA	973	107,981
	10 20 30 40 50 60 GVSSRARRPP GSSRSSRRGV TSELAWTTHP ETGWEEVSGY DEAMNPIRTY QVCNVREANQ 70 80 90 100 110 120 NNWLRTKFIQ RQDVQRVYVE LKFTVRDCNS IPNIPGSCKE TFNLFYYESD TDSASANSPF 130 140 150 160 170 180 WMENPYIKVD TIAPDESFSK LESGRVNTKV RSFGPLSKNG FYLAFQDLGA CMSLISVRAF 190 200 210 220 230 240 YKKCSNTIAG FAIFPETLTG AEPTSLVIAP GTCIPNAVEV SVPLKLYCNG DGEWMVPVGA 250 260 270 280 290 300 CTCAAGYEPA MKDTQCQACG PGTFKSKQGE GPCSPCPPNS RTTAGAATVC ICRSGFFRAD 310 320 330 340 350 360 ADPADSACTS VPSAPRSVIS NVNETSLVLE WSEPQDAGGR DDLLYNVICK KCSVERRLCS 370 380 390 400 410 420 RCDDNVEFVP RQLGLTGLTE RRIYISKVMA HPQYTFEIQA VNGISSKSPY PPHFASVNIT 430 440 450 460 470 480 TNQAAPSAVP TMHLHSSTGN SMTLSWTPPE RPNGIILDYE IKYSEKQGQG DGIANTVTSQ 490 500 510 520 530 540 KNSVRLDGLK ANARYMVQVR ARTVAGYGRY SLPTEFQTTA EDGSTSKTFQ ELPLIVGSAT 550 560 570 580 590 600 AGLLFVIVVV IIAIVCFRKQ RNSTDPEYTE KLQQYVTPGM KVYIDPFTYE DPNEAVREFA 610 620 630 640 650 660 KEIDISCVKI EEVIGAGEFG EVCRGRLKLP GRREIFVAIK TLKVGYTERQ RRDFLSEASI 670 680 690 700 710 720 MGQFDHPNII HLEGVVTKSR PVMIITEFME NCALDSFLRL NDGQFTVIQL VGMLRGIAAG 730 740 750 760 770 780 MKYLSEMNYV HRDLAARNIL VNSNLVCKVS DFGLSRFLED DPADPTYTSS LGGKIPIRWT 790 800 810 820 830 840 APEAIAYRKF TSASDVWSYG IVMWEVMSYG ERPYWDMSNQ DVINAVEQDY RLPPPMDCPT 850 860 870 880 890 900 ALHQLMLDCW VRDRNLRPKF AQIVNTLDKL IRNAASLKVI ASVQSGVSQP LLDRTVPDYT 910 920 930 940 950 960 TFTTVGDWLD AIKMGRYKEN FVNAGFASFD LVAQMTAEDL LRIGVTLAGH QKKILSSIQD 970 MRLQMNQTLP VQV

References

[1]	"Five novel avian Eph-related tyrosine kinases are differentially expressed." Sajjadi F.G., Pasquale E.B. Oncogene 8:1807-1813(1993) [PubMed: 8510926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z19061 mRNA. Translation: CAA79511.1.
IPI	IPI00572014. IPI00681373.
PIR	I50611.
UniGene	Gga.3053
3D structure databases
SMR	Q07498. Positions 21-184, 310-522, 596-893, 904-983.
ModBase	Search...
Protein-protein interaction databases
STRING	Q07498.
Genome annotation databases
Ensembl	ENSGALT00000013912; ENSGALP00000013896; ENSGALG00000008537; Gallus gallus. [Genome view] ENSGALT00000039784; ENSGALP00000038993; ENSGALG00000008537; Gallus gallus. [Genome view]
Phylogenomic databases
eggNOG	veNOG06544.
HOVERGEN	Q07498.
Enzyme and pathway databases
BRENDA	2.7.10.1. 4.
Family and domain databases
InterPro	IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR008979. Galactose-bd-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR010993. SAM_homology. IPR013761. SAM_type. IPR021129. SAM_type1. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR016257. Tyr_prot_kinase_ephrin_rcpt. IPR001426. Tyr_prot_kinase_rcpt_V_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit. G3DSA:1.10.150.50. SAM_type. 1 hit.
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF00536. SAM_1. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00014. FNTYPEIII.
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPHB3_CHICK

Accession

Primary (citable) accession number: Q07498

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)