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Q07498

- EPHB3_CHICK

UniProt

Q07498 - EPHB3_CHICK

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Protein

Ephrin type-B receptor 3

Gene

EPHB3

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. May control other aspects of development through regulation of cell migration and positioning (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei655 – 6551ATPPROSITE-ProRule annotation
Active sitei748 – 7481Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi629 – 6379ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ephrin receptor activity Source: UniProtKB

GO - Biological processi

  1. axonal fasciculation Source: UniProtKB
  2. axon guidance Source: UniProtKB
  3. cell migration Source: UniProtKB
  4. dendritic spine development Source: UniProtKB
  5. dendritic spine morphogenesis Source: UniProtKB
  6. ephrin receptor signaling pathway Source: UniProtKB
  7. positive regulation of synapse assembly Source: UniProtKB
  8. protein autophosphorylation Source: UniProtKB
  9. regulation of axonogenesis Source: UniProtKB
  10. regulation of Cdc42 GTPase activity Source: UniProtKB
  11. regulation of cell-cell adhesion Source: UniProtKB
  12. regulation of Rac GTPase activity Source: UniProtKB
  13. substrate adhesion-dependent cell spreading Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Neurogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-B receptor 3 (EC:2.7.10.1)
Alternative name(s):
EPH-like kinase 10
Short name:
EK10
Short name:
cEK10
Gene namesi
Name:EPHB3
Synonyms:CEK10
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Cell membrane By similarity; Single-pass type I membrane protein By similarity. Cell projectiondendrite By similarity

GO - Cellular componenti

  1. cell projection Source: UniProtKB-KW
  2. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 988›988Ephrin type-B receptor 3PRO_0000160276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 171By similarity
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi418 – 4181N-linked (GlcNAc...)Sequence Analysis
Modified residuei604 – 6041Phosphotyrosine; by autocatalysisBy similarity

Post-translational modificationi

Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-604 is required for interaction with SH2 domain-containing proteins (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ07498.

Expressioni

Tissue specificityi

Present in 10-day embryonic brain and body tissues. Prominent expression in kidney. Lower expression in lung, and barely detectable in brain, liver, heart, skeletal muscle and thymus.

Interactioni

Subunit structurei

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000013896.

Structurei

3D structure databases

ProteinModelPortaliQ07498.
SMRiQ07498. Positions 21-184, 596-893, 904-983.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini‹1 – 534›534ExtracellularSequence AnalysisAdd
BLAST
Topological domaini556 – 988433CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei535 – 55521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 189179Eph LBDPROSITE-ProRule annotationAdd
BLAST
Domaini311 – 424114Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini425 – 52298Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini623 – 886264Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini915 – 97965SAMPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi986 – 9883PDZ-bindingSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi171 – 308138Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
Contains 1 Eph LBD (Eph ligand-binding) domain.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233856.
HOVERGENiHBG062180.
InParanoidiQ07498.
PhylomeDBiQ07498.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. Fibronectin_type3.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEiPS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Fragment.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q07498-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
GVSSRARRPP GSSRSSRRGV TSELAWTTHP ETGWEEVSGY DEAMNPIRTY
60 70 80 90 100
QVCNVREANQ NNWLRTKFIQ RQDVQRVYVE LKFTVRDCNS IPNIPGSCKE
110 120 130 140 150
TFNLFYYESD TDSASANSPF WMENPYIKVD TIAPDESFSK LESGRVNTKV
160 170 180 190 200
RSFGPLSKNG FYLAFQDLGA CMSLISVRAF YKKCSNTIAG FAIFPETLTG
210 220 230 240 250
AEPTSLVIAP GTCIPNAVEV SVPLKLYCNG DGEWMVPVGA CTCAAGYEPA
260 270 280 290 300
MKDTQCQACG PGTFKSKQGE GPCSPCPPNS RTTAGAATVC ICRSGFFRAD
310 320 330 340 350
ADPADSACTS VPSAPRSVIS NVNETSLVLE WSEPQDAGGR DDLLYNVICK
360 370 380 390 400
KCSVERRLCS RCDDNVEFVP RQLGLTGLTE RRIYISKVMA HPQYTFEIQA
410 420 430 440 450
VNGISSKSPY PPHFASVNIT TNQAAPSAVP TMHLHSSTGN SMTLSWTPPE
460 470 480 490 500
RPNGIILDYE IKYSEKQGQG DGIANTVTSQ KNSVRLDGLK ANARYMVQVR
510 520 530 540 550
ARTVAGYGRY SLPTEFQTTA EDGSTSKTFQ ELPLIVGSAT AGLLFVIVVV
560 570 580 590 600
IIAIVCFRKG MVTEQLLSSP LGRKQRNSTD PEYTEKLQQY VTPGMKVYID
610 620 630 640 650
PFTYEDPNEA VREFAKEIDI SCVKIEEVIG AGEFGEVCRG RLKLPGRREI
660 670 680 690 700
FVAIKTLKVG YTERQRRDFL SEASIMGQFD HPNIIHLEGV VTKSRPVMII
710 720 730 740 750
TEFMENCALD SFLRLNDGQF TVIQLVGMLR GIAAGMKYLS EMNYVHRDLA
760 770 780 790 800
ARNILVNSNL VCKVSDFGLS RFLEDDPADP TYTSSLGGKI PIRWTAPEAI
810 820 830 840 850
AYRKFTSASD VWSYGIVMWE VMSYGERPYW DMSNQDVINA VEQDYRLPPP
860 870 880 890 900
MDCPTALHQL MLDCWVRDRN LRPKFAQIVN TLDKLIRNAA SLKVIASVQS
910 920 930 940 950
GVSQPLLDRT VPDYTTFTTV GDWLDAIKMG RYKENFVNAG FASFDLVAQM
960 970 980
TAEDLLRIGV TLAGHQKKIL SSIQDMRLQM NQTLPVQV
Length:988
Mass (Da):109,579
Last modified:November 1, 1996 - v1
Checksum:iEEA0D39C03FFD3C8
GO
Isoform Short (identifier: Q07498-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     558-572: Missing.

Note: No experimental confirmation available.

Show »
Length:973
Mass (Da):107,981
Checksum:i533459035D7F9200
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei558 – 57215Missing in isoform Short. 1 PublicationVSP_003019Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z19061 mRNA. Translation: CAA79511.1.
PIRiI50611.
UniGeneiGga.3053.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z19061 mRNA. Translation: CAA79511.1 .
PIRi I50611.
UniGenei Gga.3053.

3D structure databases

ProteinModelPortali Q07498.
SMRi Q07498. Positions 21-184, 596-893, 904-983.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000013896.

Proteomic databases

PaxDbi Q07498.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233856.
HOVERGENi HBG062180.
InParanoidi Q07498.
PhylomeDBi Q07498.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProi IPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. Fibronectin_type3.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view ]
Pfami PF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSi PR00109. TYRKINASE.
SMARTi SM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEi PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Five novel avian Eph-related tyrosine kinases are differentially expressed."
    Sajjadi F.G., Pasquale E.B.
    Oncogene 8:1807-1813(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Tissue: Embryo.

Entry informationi

Entry nameiEPHB3_CHICK
AccessioniPrimary (citable) accession number: Q07498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3