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Q07498

- EPHB3_CHICK

UniProt

Q07498 - EPHB3_CHICK

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Protein

Ephrin type-B receptor 3

Gene
EPHB3, CEK10
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. May control other aspects of development through regulation of cell migration and positioning By similarity.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei655 – 6551ATP By similarity
Active sitei748 – 7481Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi629 – 6379ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ephrin receptor activity Source: UniProtKB

GO - Biological processi

  1. axonal fasciculation Source: UniProtKB
  2. axon guidance Source: UniProtKB
  3. cell migration Source: UniProtKB
  4. dendritic spine development Source: UniProtKB
  5. dendritic spine morphogenesis Source: UniProtKB
  6. ephrin receptor signaling pathway Source: UniProtKB
  7. positive regulation of synapse assembly Source: UniProtKB
  8. protein autophosphorylation Source: UniProtKB
  9. regulation of axonogenesis Source: UniProtKB
  10. regulation of Cdc42 GTPase activity Source: UniProtKB
  11. regulation of cell-cell adhesion Source: UniProtKB
  12. regulation of Rac GTPase activity Source: UniProtKB
  13. substrate adhesion-dependent cell spreading Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Neurogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-B receptor 3 (EC:2.7.10.1)
Alternative name(s):
EPH-like kinase 10
Short name:
EK10
Short name:
cEK10
Gene namesi
Name:EPHB3
Synonyms:CEK10
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini‹1 – 534›534Extracellular Reviewed predictionAdd
BLAST
Transmembranei535 – 55521Helical; Reviewed predictionAdd
BLAST
Topological domaini556 – 988433Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. dendrite Source: UniProtKB-SubCell
  2. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 988›988Ephrin type-B receptor 3PRO_0000160276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 171 By similarity
Glycosylationi323 – 3231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi418 – 4181N-linked (GlcNAc...) Reviewed prediction
Modified residuei604 – 6041Phosphotyrosine; by autocatalysis By similarity

Post-translational modificationi

Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-604 is required for interaction with SH2 domain-containing proteins By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ07498.

Expressioni

Tissue specificityi

Present in 10-day embryonic brain and body tissues. Prominent expression in kidney. Lower expression in lung, and barely detectable in brain, liver, heart, skeletal muscle and thymus.

Interactioni

Subunit structurei

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses By similarity.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000013896.

Structurei

3D structure databases

ProteinModelPortaliQ07498.
SMRiQ07498. Positions 21-184, 596-893, 904-983.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 189179Eph LBDAdd
BLAST
Domaini311 – 424114Fibronectin type-III 1Add
BLAST
Domaini425 – 52298Fibronectin type-III 2Add
BLAST
Domaini623 – 886264Protein kinaseAdd
BLAST
Domaini915 – 97965SAMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi986 – 9883PDZ-binding Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi171 – 308138Cys-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233856.
HOVERGENiHBG062180.
PhylomeDBiQ07498.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. Fibronectin_type3.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEiPS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Fragment.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q07498-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

GVSSRARRPP GSSRSSRRGV TSELAWTTHP ETGWEEVSGY DEAMNPIRTY    50
QVCNVREANQ NNWLRTKFIQ RQDVQRVYVE LKFTVRDCNS IPNIPGSCKE 100
TFNLFYYESD TDSASANSPF WMENPYIKVD TIAPDESFSK LESGRVNTKV 150
RSFGPLSKNG FYLAFQDLGA CMSLISVRAF YKKCSNTIAG FAIFPETLTG 200
AEPTSLVIAP GTCIPNAVEV SVPLKLYCNG DGEWMVPVGA CTCAAGYEPA 250
MKDTQCQACG PGTFKSKQGE GPCSPCPPNS RTTAGAATVC ICRSGFFRAD 300
ADPADSACTS VPSAPRSVIS NVNETSLVLE WSEPQDAGGR DDLLYNVICK 350
KCSVERRLCS RCDDNVEFVP RQLGLTGLTE RRIYISKVMA HPQYTFEIQA 400
VNGISSKSPY PPHFASVNIT TNQAAPSAVP TMHLHSSTGN SMTLSWTPPE 450
RPNGIILDYE IKYSEKQGQG DGIANTVTSQ KNSVRLDGLK ANARYMVQVR 500
ARTVAGYGRY SLPTEFQTTA EDGSTSKTFQ ELPLIVGSAT AGLLFVIVVV 550
IIAIVCFRKG MVTEQLLSSP LGRKQRNSTD PEYTEKLQQY VTPGMKVYID 600
PFTYEDPNEA VREFAKEIDI SCVKIEEVIG AGEFGEVCRG RLKLPGRREI 650
FVAIKTLKVG YTERQRRDFL SEASIMGQFD HPNIIHLEGV VTKSRPVMII 700
TEFMENCALD SFLRLNDGQF TVIQLVGMLR GIAAGMKYLS EMNYVHRDLA 750
ARNILVNSNL VCKVSDFGLS RFLEDDPADP TYTSSLGGKI PIRWTAPEAI 800
AYRKFTSASD VWSYGIVMWE VMSYGERPYW DMSNQDVINA VEQDYRLPPP 850
MDCPTALHQL MLDCWVRDRN LRPKFAQIVN TLDKLIRNAA SLKVIASVQS 900
GVSQPLLDRT VPDYTTFTTV GDWLDAIKMG RYKENFVNAG FASFDLVAQM 950
TAEDLLRIGV TLAGHQKKIL SSIQDMRLQM NQTLPVQV 988
Length:988
Mass (Da):109,579
Last modified:November 1, 1996 - v1
Checksum:iEEA0D39C03FFD3C8
GO
Isoform Short (identifier: Q07498-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     558-572: Missing.

Note: No experimental confirmation available.

Show »
Length:973
Mass (Da):107,981
Checksum:i533459035D7F9200
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei558 – 57215Missing in isoform Short. VSP_003019Add
BLAST

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z19061 mRNA. Translation: CAA79511.1.
PIRiI50611.
UniGeneiGga.3053.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z19061 mRNA. Translation: CAA79511.1 .
PIRi I50611.
UniGenei Gga.3053.

3D structure databases

ProteinModelPortali Q07498.
SMRi Q07498. Positions 21-184, 596-893, 904-983.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000013896.

Proteomic databases

PaxDbi Q07498.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233856.
HOVERGENi HBG062180.
PhylomeDBi Q07498.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProi IPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. Fibronectin_type3.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view ]
Pfami PF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSi PR00109. TYRKINASE.
SMARTi SM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEi PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Five novel avian Eph-related tyrosine kinases are differentially expressed."
    Sajjadi F.G., Pasquale E.B.
    Oncogene 8:1807-1813(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Tissue: Embryo.

Entry informationi

Entry nameiEPHB3_CHICK
AccessioniPrimary (citable) accession number: Q07498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi