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Protein

Blue copper protein

Gene

BCB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably acts as an electron carrier.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661CopperPROSITE-ProRule annotation
Metal bindingi107 – 1071CopperPROSITE-ProRule annotation
Metal bindingi112 – 1121CopperPROSITE-ProRule annotation
Metal bindingi117 – 1171CopperPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • aluminum cation transport Source: TAIR
  • cellular response to cold Source: TAIR
  • defense response to fungus Source: TAIR
  • oxidation-reduction process Source: UniProtKB-KW
  • regulation of lignin biosynthetic process Source: TAIR
  • response to absence of light Source: TAIR
  • response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Blue copper protein
Alternative name(s):
Blue copper-binding protein
Short name:
AtBCB
Phytocyanin 1
Stellacyanin
Gene namesi
Name:BCB
Synonyms:AWI 32
Ordered Locus Names:At5g20230
ORF Names:F5O24.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G20230.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • plasma membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 174152Blue copper proteinPRO_0000002866Add
BLAST
Propeptidei175 – 19622Removed in mature formCuratedPRO_0000002867Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi79 ↔ 113PROSITE-ProRule annotation
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence analysis
Lipidationi174 – 1741GPI-anchor amidated asparagineCurated

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ07488.
PRIDEiQ07488.

Expressioni

Developmental stagei

Maximum levels are found in 35 day old plantlets when the rosette is mature, consisting of 8-10 fully expanded leaves, and as the floral stem starts to form. This level remains constant during the further life span of the plant.

Inductioni

By dark adaptation. This gives a 20-fold increase in expression.

Gene expression databases

GenevisibleiQ07488. AT.

Interactioni

Protein-protein interaction databases

BioGridi17421. 63 interactions.
STRINGi3702.AT5G20230.1.

Structurei

3D structure databases

ProteinModelPortaliQ07488.
SMRiQ07488. Positions 23-126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 125103PhytocyaninPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 phytocyanin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JEYN. Eukaryota.
ENOG410Z0W4. LUCA.
HOGENOMiHOG000237627.
InParanoidiQ07488.
OMAiSEYFICT.
PhylomeDBiQ07488.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR028871. BlueCu_1_BS.
IPR008972. Cupredoxin.
IPR003245. Phytocyanin_dom.
[Graphical view]
PfamiPF02298. Cu_bind_like. 1 hit.
[Graphical view]
ProDomiPD003122. Plcyanin-like. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS00196. COPPER_BLUE. 1 hit.
PS51485. PHYTOCYANIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVFKTVTF LVLVFAAVVV FAEDYDVGDD TEWTRPMDPE FYTTWATGKT
60 70 80 90 100
FRVGDELEFD FAAGRHDVAV VSEAAFENCE KEKPISHMTV PPVKIMLNTT
110 120 130 140 150
GPQYFICTVG DHCRFGQKLS ITVVAAGATG GATPGAGATP APGSTPSTGG
160 170 180 190
TTPPTAGGTT TPSGSSGTTT PAGNAASSLG GATFLVAFVS AVVALF
Length:196
Mass (Da):20,054
Last modified:May 27, 2002 - v2
Checksum:i05100B50518F0A56
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441T → S in CAA78771 (PubMed:8294044).Curated
Sequence conflicti134 – 1341P → L in CAA78771 (PubMed:8294044).Curated
Sequence conflicti142 – 1421P → L in CAA78771 (PubMed:8294044).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z15058 Genomic DNA. Translation: CAA78771.1.
Y18227 mRNA. Translation: CAA77089.1.
AB035137 Genomic DNA. Translation: BAA86999.1.
AF296825 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92816.1.
AY052681 mRNA. Translation: AAK96585.1.
AY034986 mRNA. Translation: AAK59491.1.
AY142577 mRNA. Translation: AAN13146.1.
AY088549 mRNA. Translation: AAM66081.1.
PIRiI39698.
T51838.
RefSeqiNP_197523.1. NM_122030.3.
UniGeneiAt.20630.

Genome annotation databases

EnsemblPlantsiAT5G20230.1; AT5G20230.1; AT5G20230.
GeneIDi832145.
GrameneiAT5G20230.1; AT5G20230.1; AT5G20230.
KEGGiath:AT5G20230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z15058 Genomic DNA. Translation: CAA78771.1.
Y18227 mRNA. Translation: CAA77089.1.
AB035137 Genomic DNA. Translation: BAA86999.1.
AF296825 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92816.1.
AY052681 mRNA. Translation: AAK96585.1.
AY034986 mRNA. Translation: AAK59491.1.
AY142577 mRNA. Translation: AAN13146.1.
AY088549 mRNA. Translation: AAM66081.1.
PIRiI39698.
T51838.
RefSeqiNP_197523.1. NM_122030.3.
UniGeneiAt.20630.

3D structure databases

ProteinModelPortaliQ07488.
SMRiQ07488. Positions 23-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17421. 63 interactions.
STRINGi3702.AT5G20230.1.

Proteomic databases

PaxDbiQ07488.
PRIDEiQ07488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G20230.1; AT5G20230.1; AT5G20230.
GeneIDi832145.
GrameneiAT5G20230.1; AT5G20230.1; AT5G20230.
KEGGiath:AT5G20230.

Organism-specific databases

TAIRiAT5G20230.

Phylogenomic databases

eggNOGiENOG410JEYN. Eukaryota.
ENOG410Z0W4. LUCA.
HOGENOMiHOG000237627.
InParanoidiQ07488.
OMAiSEYFICT.
PhylomeDBiQ07488.

Miscellaneous databases

PROiQ07488.

Gene expression databases

GenevisibleiQ07488. AT.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR028871. BlueCu_1_BS.
IPR008972. Cupredoxin.
IPR003245. Phytocyanin_dom.
[Graphical view]
PfamiPF02298. Cu_bind_like. 1 hit.
[Graphical view]
ProDomiPD003122. Plcyanin-like. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS00196. COPPER_BLUE. 1 hit.
PS51485. PHYTOCYANIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A negatively light-regulated gene from Arabidopsis thaliana encodes a protein showing high similarity to blue copper-binding proteins."
    van Gysel A., van Montagu M., Inze D.
    Gene 136:79-85(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia and cv. Columbia K85.
  2. "Characterization of a wound-inducible Arabidopsis gene encoding a protein homologous to blue copper binding proteins."
    Yang K.Y., Kim C.S., Cho B.H.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Wassilewskija.
  3. "The Arabidopsis floral homeotic gene PISTILLATA is regulated by discrete cis-elements responsive to induction and maintenance signals."
    Honma T., Goto K.
    Development 127:2021-2030(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  4. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "Uclacyanins, stellacyanins, and plantacyanins are distinct subfamilies of phytocyanins: plant-specific mononuclear blue copper proteins."
    Nersissian A.M., Immoos C., Hill M.G., Hart P.J., Williams G., Herrmann R.G., Valentine J.S.
    Protein Sci. 7:1915-1929(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
    Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
    Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
    Tissue: Callus.
  10. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
  11. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
    Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
    J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBCB1_ARATH
AccessioniPrimary (citable) accession number: Q07488
Secondary accession number(s): O82664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 27, 2002
Last modified: February 17, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.