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Q07468

- VAM6_YEAST

UniProt

Q07468 - VAM6_YEAST

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Protein
Vacuolar morphogenesis protein 6
Gene
VAM6, CVT4, VPL18, VPL22, VPS39, YDL077C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for vacuolar assembly. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.1 Publication

GO - Molecular functioni

  1. Rab guanyl-nucleotide exchange factor activity Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. intracellular protein transport Source: InterPro
  2. piecemeal microautophagy of nucleus Source: SGD
  3. regulation of Rab GTPase activity Source: GOC
  4. regulation of SNARE complex assembly Source: SGD
  5. regulation of vacuole fusion, non-autophagic Source: SGD
  6. vacuole fusion, non-autophagic Source: SGD
  7. vacuole organization Source: SGD
  8. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29488-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar morphogenesis protein 6
Alternative name(s):
Vacuolar protein sorting-associated protein 39
Gene namesi
Name:VAM6
Synonyms:CVT4, VPL18, VPL22, VPS39
Ordered Locus Names:YDL077C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL077c.
SGDiS000002235. VAM6.

Subcellular locationi

GO - Cellular componenti

  1. HOPS complex Source: SGD
  2. fungal-type vacuole Source: SGD
  3. fungal-type vacuole membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10491049Vacuolar morphogenesis protein 6
PRO_0000065903Add
BLAST

Proteomic databases

MaxQBiQ07468.
PaxDbiQ07468.
PeptideAtlasiQ07468.

Expressioni

Gene expression databases

GenevestigatoriQ07468.

Interactioni

Subunit structurei

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with VPS41 and VAM7.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS33P207954EBI-20422,EBI-20395

Protein-protein interaction databases

BioGridi31984. 180 interactions.
DIPiDIP-1002N.
IntActiQ07468. 10 interactions.
MINTiMINT-423868.
STRINGi4932.YDL077C.

Structurei

3D structure databases

ProteinModelPortaliQ07468.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati730 – 896167CHCR
Add
BLAST

Sequence similaritiesi

Belongs to the VAM6/VPS39 family.

Phylogenomic databases

eggNOGiNOG270269.
GeneTreeiENSGT00530000063596.
HOGENOMiHOG000154806.
OMAiWAPPQLV.
OrthoDBiEOG72G1H6.

Family and domain databases

InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
IPR019452. VPS39/TGF_beta_rcpt-assoc_1.
IPR019453. VPS39/TGF_beta_rcpt-assoc_2.
[Graphical view]
PfamiPF10366. Vps39_1. 1 hit.
PF10367. Vps39_2. 1 hit.
[Graphical view]
PROSITEiPS50236. CHCR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07468-1 [UniParc]FASTAAdd to Basket

« Hide

MLRAQKLHSL KSSDITAILP TEQSQKLVLA KKNGDVEVYS RDGNTLKLFQ     50
VYPDLLQNAK NDPLPPVIEN FYFANELSTI FAQCKETLIL LSTTNLHEYD 100
RIIDRRGINH CWLFERSHKN KEEKNTYLIY STINTAKMRV LIWEGRTYKN 150
MMEASLSYRK ETIRSIYPGE TGITLATDLG IYHWPYNKPS LIRIEKTVKN 200
KFPKDMISAL TELKEQAEKV IEKKPKKNSH FDAQSFSSMD RMSRKSSMSS 250
LWYRTIRNER GNKIRYTFEL DGNDATPMII DGATKKIFKV ELMHNNEEPF 300
LIATDHATFS ESNSEFDHMQ YLSSNLLMLY NSSTIKFVDY ENGFTFLQQK 350
IPEGIKWVKN LSGTYFLVWT SNDEVQLFSY HVDDGSEDDD QESICGDIND 400
PDFYQLWRKV LFYKFFIDSP HSKELCVSDN PEESLDICAM KLRDLTVMWC 450
LRIFDKFQNY MVQLERSRNS RMIRSKCEEM IIKSIFDLFI KFWAPPQLVI 500
LKVFPSAISS LVLEITGQEH HCLLKEAEEV KETYDIPPHL LNRWCLPYLT 550
DTRRHLQNLL SKENDDESRI TWCYRDREIK QSFDFFLISN HDDVDLNTML 600
TLIDTVLFKC YLYYNPPMVG PFIRVENHCD SHVIVTELKI RHMFKDLIDF 650
YYKRGNHEEA LKFLTDLVDE LENDNTDQKQ RQKIDHGVKI LVIYYLKKLS 700
NPQLDVIFTY TDWLLNRHND SIKEILSSIF FYDSQACSSR DHLKVYGYIK 750
KFDKLLAIQY LEFAISTFRL EGNKLHTVLI KLYLENLDIP STRIKLKSLL 800
ETTSVYEPRT ILKLLNDAIE SGSDQLPTNQ LNFVKYLKIF PLSKLENHKE 850
AVHILLDEID DYKAATSYCN DVYQSDSTKG EELLLYLYSK LVSIYDSNRN 900
SKLILNFLQD HGSKLNSAEI YKNLPQDISL YDIGRVVSQL LKKHTSKMDE 950
TRLEKALLQV ELVATTYKLN ERMSSYGVLS DSHKCPICKK VISNFGTDSI 1000
SWFTREGRNI ITHYNCGKVL QERFNAKNEK SSRIKQKTLG EVINELNNK 1049
Length:1,049
Mass (Da):122,882
Last modified:November 1, 1996 - v1
Checksum:iEE8D94E7E2F5F078
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74125 Genomic DNA. Translation: CAA98643.1.
D83058 Genomic DNA. Translation: BAA11758.1.
BK006938 Genomic DNA. Translation: DAA11782.1.
PIRiS67613.
RefSeqiNP_010206.1. NM_001180136.1.

Genome annotation databases

EnsemblFungiiYDL077C; YDL077C; YDL077C.
GeneIDi851482.
KEGGisce:YDL077C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74125 Genomic DNA. Translation: CAA98643.1 .
D83058 Genomic DNA. Translation: BAA11758.1 .
BK006938 Genomic DNA. Translation: DAA11782.1 .
PIRi S67613.
RefSeqi NP_010206.1. NM_001180136.1.

3D structure databases

ProteinModelPortali Q07468.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31984. 180 interactions.
DIPi DIP-1002N.
IntActi Q07468. 10 interactions.
MINTi MINT-423868.
STRINGi 4932.YDL077C.

Proteomic databases

MaxQBi Q07468.
PaxDbi Q07468.
PeptideAtlasi Q07468.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL077C ; YDL077C ; YDL077C .
GeneIDi 851482.
KEGGi sce:YDL077C.

Organism-specific databases

CYGDi YDL077c.
SGDi S000002235. VAM6.

Phylogenomic databases

eggNOGi NOG270269.
GeneTreei ENSGT00530000063596.
HOGENOMi HOG000154806.
OMAi WAPPQLV.
OrthoDBi EOG72G1H6.

Enzyme and pathway databases

BioCyci YEAST:G3O-29488-MONOMER.

Miscellaneous databases

NextBioi 968798.

Gene expression databases

Genevestigatori Q07468.

Family and domain databases

InterProi IPR000547. Clathrin_H-chain/VPS_repeat.
IPR019452. VPS39/TGF_beta_rcpt-assoc_1.
IPR019453. VPS39/TGF_beta_rcpt-assoc_2.
[Graphical view ]
Pfami PF10366. Vps39_1. 1 hit.
PF10367. Vps39_2. 1 hit.
[Graphical view ]
PROSITEi PS50236. CHCR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Vam2/Vps41p and Vam6/Vps39p are components of a protein complex on the vacuolar membranes and involved in the vacuolar assembly in the yeast Saccharomyces cerevisiae."
    Nakamura N., Hirata A., Ohsumi Y., Wada Y.
    J. Biol. Chem. 272:11344-11349(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
    Stroupe C., Collins K.M., Fratti R.A., Wickner W.
    EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVAM6_YEAST
AccessioniPrimary (citable) accession number: Q07468
Secondary accession number(s): D6VRS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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