Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q07468 (VAM6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar morphogenesis protein 6
Alternative name(s):
Vacuolar protein sorting-associated protein 39
Gene names
Name:VAM6
Synonyms:CVT4, VPL18, VPL22, VPS39
Ordered Locus Names:YDL077C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1049 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for vacuolar assembly. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Ref.5

Subunit structure

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with VPS41 and VAM7. Ref.5

Subcellular location

Vacuole membrane; Peripheral membrane protein Potential.

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the VAM6/VPS39 family.

Contains 1 CHCR (clathrin heavy-chain) repeat.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VPS33P207954EBI-20422,EBI-20395

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10491049Vacuolar morphogenesis protein 6
PRO_0000065903

Regions

Repeat730 – 896167CHCR

Sequences

Sequence LengthMass (Da)Tools
Q07468 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EE8D94E7E2F5F078

FASTA1,049122,882
        10         20         30         40         50         60 
MLRAQKLHSL KSSDITAILP TEQSQKLVLA KKNGDVEVYS RDGNTLKLFQ VYPDLLQNAK 

        70         80         90        100        110        120 
NDPLPPVIEN FYFANELSTI FAQCKETLIL LSTTNLHEYD RIIDRRGINH CWLFERSHKN 

       130        140        150        160        170        180 
KEEKNTYLIY STINTAKMRV LIWEGRTYKN MMEASLSYRK ETIRSIYPGE TGITLATDLG 

       190        200        210        220        230        240 
IYHWPYNKPS LIRIEKTVKN KFPKDMISAL TELKEQAEKV IEKKPKKNSH FDAQSFSSMD 

       250        260        270        280        290        300 
RMSRKSSMSS LWYRTIRNER GNKIRYTFEL DGNDATPMII DGATKKIFKV ELMHNNEEPF 

       310        320        330        340        350        360 
LIATDHATFS ESNSEFDHMQ YLSSNLLMLY NSSTIKFVDY ENGFTFLQQK IPEGIKWVKN 

       370        380        390        400        410        420 
LSGTYFLVWT SNDEVQLFSY HVDDGSEDDD QESICGDIND PDFYQLWRKV LFYKFFIDSP 

       430        440        450        460        470        480 
HSKELCVSDN PEESLDICAM KLRDLTVMWC LRIFDKFQNY MVQLERSRNS RMIRSKCEEM 

       490        500        510        520        530        540 
IIKSIFDLFI KFWAPPQLVI LKVFPSAISS LVLEITGQEH HCLLKEAEEV KETYDIPPHL 

       550        560        570        580        590        600 
LNRWCLPYLT DTRRHLQNLL SKENDDESRI TWCYRDREIK QSFDFFLISN HDDVDLNTML 

       610        620        630        640        650        660 
TLIDTVLFKC YLYYNPPMVG PFIRVENHCD SHVIVTELKI RHMFKDLIDF YYKRGNHEEA 

       670        680        690        700        710        720 
LKFLTDLVDE LENDNTDQKQ RQKIDHGVKI LVIYYLKKLS NPQLDVIFTY TDWLLNRHND 

       730        740        750        760        770        780 
SIKEILSSIF FYDSQACSSR DHLKVYGYIK KFDKLLAIQY LEFAISTFRL EGNKLHTVLI 

       790        800        810        820        830        840 
KLYLENLDIP STRIKLKSLL ETTSVYEPRT ILKLLNDAIE SGSDQLPTNQ LNFVKYLKIF 

       850        860        870        880        890        900 
PLSKLENHKE AVHILLDEID DYKAATSYCN DVYQSDSTKG EELLLYLYSK LVSIYDSNRN 

       910        920        930        940        950        960 
SKLILNFLQD HGSKLNSAEI YKNLPQDISL YDIGRVVSQL LKKHTSKMDE TRLEKALLQV 

       970        980        990       1000       1010       1020 
ELVATTYKLN ERMSSYGVLS DSHKCPICKK VISNFGTDSI SWFTREGRNI ITHYNCGKVL 

      1030       1040 
QERFNAKNEK SSRIKQKTLG EVINELNNK 

« Hide

References

« Hide 'large scale' references
[1]"Vam2/Vps41p and Vam6/Vps39p are components of a protein complex on the vacuolar membranes and involved in the vacuolar assembly in the yeast Saccharomyces cerevisiae."
Nakamura N., Hirata A., Ohsumi Y., Wada Y.
J. Biol. Chem. 272:11344-11349(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
Stroupe C., Collins K.M., Fratti R.A., Wickner W.
EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
[6]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z74125 Genomic DNA. Translation: CAA98643.1.
D83058 Genomic DNA. Translation: BAA11758.1.
BK006938 Genomic DNA. Translation: DAA11782.1.
PIRS67613.
RefSeqNP_010206.1. NM_001180136.1.

3D structure databases

ProteinModelPortalQ07468.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31984. 180 interactions.
DIPDIP-1002N.
IntActQ07468. 10 interactions.
MINTMINT-423868.
STRING4932.YDL077C.

Proteomic databases

MaxQBQ07468.
PaxDbQ07468.
PeptideAtlasQ07468.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL077C; YDL077C; YDL077C.
GeneID851482.
KEGGsce:YDL077C.

Organism-specific databases

CYGDYDL077c.
SGDS000002235. VAM6.

Phylogenomic databases

eggNOGNOG270269.
GeneTreeENSGT00530000063596.
HOGENOMHOG000154806.
OMAWAPPQLV.
OrthoDBEOG72G1H6.

Enzyme and pathway databases

BioCycYEAST:G3O-29488-MONOMER.

Gene expression databases

GenevestigatorQ07468.

Family and domain databases

InterProIPR000547. Clathrin_H-chain/VPS_repeat.
IPR019452. VPS39/TGF_beta_rcpt-assoc_1.
IPR019453. VPS39/TGF_beta_rcpt-assoc_2.
[Graphical view]
PfamPF10366. Vps39_1. 1 hit.
PF10367. Vps39_2. 1 hit.
[Graphical view]
PROSITEPS50236. CHCR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968798.

Entry information

Entry nameVAM6_YEAST
AccessionPrimary (citable) accession number: Q07468
Secondary accession number(s): D6VRS2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families