Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase BRE1

Gene

BRE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2B to form H2BK123ub1 in association with the E2 enzyme RAD6/UBC2. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. It thereby plays a central role in histone code and gene regulation. Also modulates the formation of double-strand breaks during meiosis.5 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri648 – 687RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • DNA replication origin binding Source: SGD
  • identical protein binding Source: IntAct
  • ubiquitin-protein transferase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin silencing at telomere Source: SGD
  • double-strand break repair via homologous recombination Source: SGD
  • histone monoubiquitination Source: SGD
  • histone ubiquitination Source: SGD
  • intra-S DNA damage checkpoint Source: SGD
  • meiotic DNA double-strand break formation Source: SGD
  • mitotic G1 DNA damage checkpoint Source: SGD
  • protein K63-linked ubiquitination Source: SGD
  • regulation of DNA-dependent DNA replication initiation Source: SGD
  • telomere maintenance via recombination Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29485-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1 (EC:2.3.2.271 Publication)
Alternative name(s):
Brefeldin A-sensitivity protein 1
RING-type E3 ubiquitin transferase BRE1Curated
Gene namesi
Name:BRE1
Ordered Locus Names:YDL074C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL074C.
SGDiS000002232. BRE1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi663C → A: Abolishes ability to monoubiquitinate histone H2B. 1 Publication1
Mutagenesisi665H → A: Abolishes ability to monoubiquitinate histone H2B. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558571 – 700E3 ubiquitin-protein ligase BRE1Add BLAST700

Proteomic databases

MaxQBiQ07457.
PRIDEiQ07457.

PTM databases

iPTMnetiQ07457.

Interactioni

Subunit structurei

Forms a complex with the E2 enzyme RAD6/UBC2. May interact with LGE1.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-31563,EBI-31563
RAD6P061044EBI-31563,EBI-19722

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi31987. 404 interactors.
DIPiDIP-2999N.
IntActiQ07457. 72 interactors.
MINTiMINT-434946.

Structurei

Secondary structure

1700
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi633 – 646Combined sources14
Turni649 – 651Combined sources3
Beta strandi652 – 655Combined sources4
Beta strandi658 – 660Combined sources3
Turni661 – 663Combined sources3
Helixi669 – 677Combined sources9
Turni684 – 686Combined sources3
Helixi692 – 694Combined sources3
Beta strandi695 – 697Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4R7EX-ray2.25A632-700[»]
ProteinModelPortaliQ07457.
SMRiQ07457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili126 – 406Sequence analysisAdd BLAST281
Coiled coili555 – 620Sequence analysisAdd BLAST66

Sequence similaritiesi

Belongs to the BRE1 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri648 – 687RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000002866.
HOGENOMiHOG000095255.
InParanoidiQ07457.
KOiK10696.
OMAiIAVANKQ.
OrthoDBiEOG092C0ULK.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR013956. E3_ubiquit_lig_BRE1.
IPR007087. Znf_C2H2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08647. BRE1. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAEPATKKI KLELSDPSEP LTQSDVIAFQ KEALFRCINR RRVDFEALRK
60 70 80 90 100
QYELSRRECI DVSRKLANIM ALIVTLARFI ETFCTDANEK QLCREIAQGD
110 120 130 140 150
ETLIVQRSDS FMKLLTKYGK PNTTDSNTNS NASDHIQELT TELKNLRKSK
160 170 180 190 200
EELFYENSQL TEEISALKEY YTNIIRKYDR DESFTIKRVF KEDKTDAVKE
210 220 230 240 250
LREDEKESNE NNIKSGNKDS SAINGDNTSK KSEKGDELVQ AEDERKEDAE
260 270 280 290 300
NEKLELDLKF SDLRAEINSL SSTIKDLENI RRENEEELIK TRSEVSNLKK
310 320 330 340 350
QQIAAADQDP DFKSYDHESL LAKIQHLTEQ NAELSEINSS FLSKFQVLAK
360 370 380 390 400
EKEIYTKKVR EEFQKSLDSL VEMNSSLEKD VVRIRTARDD LLSKIAILEA
410 420 430 440 450
EKSKTEVLSD LQHAIDILKE QWTKIDQRSN DTKSSSTQDA LIKEIQDLEK
460 470 480 490 500
GFRELSDLTH KKYSEIINHE SVISKLTVEK TKADQKYFAA MRSKDSILIE
510 520 530 540 550
IKTLSKSLSK SNELILQLKD SDRLLQQKIG NLHKQLDLSQ NNERRLIDSS
560 570 580 590 600
KTETLKIIDL NNTSTKLKRS LEKLQEESNK SIADMTHLET KLNDTEIELK
610 620 630 640 650
HFKQKASHLE SKCEKLHDTL FRGNNKNKGS SDEALVEELA NFRTLVYCSL
660 670 680 690 700
CSKNWKNMAI KTCGHVFCEN CCKERLAARM RKCPTCNKAF SSNDLLTVHL
Length:700
Mass (Da):80,692
Last modified:November 1, 1996 - v1
Checksum:iFC6B32825970D36F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74122 Genomic DNA. Translation: CAA98640.1.
BK006938 Genomic DNA. Translation: DAA11785.1.
PIRiS67610.
RefSeqiNP_010209.1. NM_001180133.1.

Genome annotation databases

EnsemblFungiiYDL074C; YDL074C; YDL074C.
GeneIDi851485.
KEGGisce:YDL074C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74122 Genomic DNA. Translation: CAA98640.1.
BK006938 Genomic DNA. Translation: DAA11785.1.
PIRiS67610.
RefSeqiNP_010209.1. NM_001180133.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4R7EX-ray2.25A632-700[»]
ProteinModelPortaliQ07457.
SMRiQ07457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31987. 404 interactors.
DIPiDIP-2999N.
IntActiQ07457. 72 interactors.
MINTiMINT-434946.

PTM databases

iPTMnetiQ07457.

Proteomic databases

MaxQBiQ07457.
PRIDEiQ07457.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL074C; YDL074C; YDL074C.
GeneIDi851485.
KEGGisce:YDL074C.

Organism-specific databases

EuPathDBiFungiDB:YDL074C.
SGDiS000002232. BRE1.

Phylogenomic databases

GeneTreeiENSGT00390000002866.
HOGENOMiHOG000095255.
InParanoidiQ07457.
KOiK10696.
OMAiIAVANKQ.
OrthoDBiEOG092C0ULK.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-29485-MONOMER.

Miscellaneous databases

PROiQ07457.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR013956. E3_ubiquit_lig_BRE1.
IPR007087. Znf_C2H2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08647. BRE1. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRE1_YEAST
AccessioniPrimary (citable) accession number: Q07457
Secondary accession number(s): D6VRS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2980 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.