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Q07457

- BRE1_YEAST

UniProt

Q07457 - BRE1_YEAST

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Protein
E3 ubiquitin-protein ligase BRE1
Gene
BRE1, YDL074C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2B to form H2BK123ub1 in association with the E2 enzyme RAD6/UBC2. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. It thereby plays a central role in histone code and gene regulation. Also modulates the formation of double-strand breaks during meiosis.5 Publications

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri648 – 68740RING-type
Add
BLAST

GO - Molecular functioni

  1. DNA replication origin binding Source: SGD
  2. identical protein binding Source: IntAct
  3. ligase activity Source: UniProtKB-KW
  4. protein binding Source: IntAct
  5. ubiquitin-protein transferase activity Source: SGD
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. chromatin silencing at telomere Source: SGD
  2. double-strand break repair via homologous recombination Source: SGD
  3. histone monoubiquitination Source: SGD
  4. histone ubiquitination Source: SGD
  5. intra-S DNA damage checkpoint Source: SGD
  6. meiotic DNA double-strand break formation Source: SGD
  7. mitotic G1 DNA damage checkpoint Source: SGD
  8. regulation of DNA-dependent DNA replication initiation Source: SGD
  9. transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29485-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1 (EC:6.3.2.-)
Alternative name(s):
Brefeldin A-sensitivity protein 1
Gene namesi
Name:BRE1
Ordered Locus Names:YDL074C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL074c.
SGDiS000002232. BRE1.

Subcellular locationi

Nucleus Inferred 1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi663 – 6631C → A: Abolishes ability to monoubiquitinate histone H2B. 1 Publication
Mutagenesisi665 – 6651H → A: Abolishes ability to monoubiquitinate histone H2B. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 700700E3 ubiquitin-protein ligase BRE1
PRO_0000055857Add
BLAST

Proteomic databases

MaxQBiQ07457.
PaxDbiQ07457.
PeptideAtlasiQ07457.
PRIDEiQ07457.

Expressioni

Gene expression databases

GenevestigatoriQ07457.

Interactioni

Subunit structurei

Forms a complex with the E2 enzyme RAD6/UBC2. May interact with LGE1.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-31563,EBI-31563
RAD6P061044EBI-31563,EBI-19722

Protein-protein interaction databases

BioGridi31987. 396 interactions.
DIPiDIP-2999N.
IntActiQ07457. 72 interactions.
MINTiMINT-434946.
STRINGi4932.YDL074C.

Structurei

3D structure databases

ProteinModelPortaliQ07457.
SMRiQ07457. Positions 647-672.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili126 – 406281 Reviewed prediction
Add
BLAST
Coiled coili555 – 62066 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the BRE1 family.

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiNOG263074.
GeneTreeiENSGT00390000002866.
HOGENOMiHOG000095255.
KOiK10696.
OMAiCENCCKE.
OrthoDBiEOG7J9W0C.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR013956. E3_ubiquit_lig_BRE1.
IPR007087. Znf_C2H2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08647. BRE1. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07457-1 [UniParc]FASTAAdd to Basket

« Hide

MTAEPATKKI KLELSDPSEP LTQSDVIAFQ KEALFRCINR RRVDFEALRK    50
QYELSRRECI DVSRKLANIM ALIVTLARFI ETFCTDANEK QLCREIAQGD 100
ETLIVQRSDS FMKLLTKYGK PNTTDSNTNS NASDHIQELT TELKNLRKSK 150
EELFYENSQL TEEISALKEY YTNIIRKYDR DESFTIKRVF KEDKTDAVKE 200
LREDEKESNE NNIKSGNKDS SAINGDNTSK KSEKGDELVQ AEDERKEDAE 250
NEKLELDLKF SDLRAEINSL SSTIKDLENI RRENEEELIK TRSEVSNLKK 300
QQIAAADQDP DFKSYDHESL LAKIQHLTEQ NAELSEINSS FLSKFQVLAK 350
EKEIYTKKVR EEFQKSLDSL VEMNSSLEKD VVRIRTARDD LLSKIAILEA 400
EKSKTEVLSD LQHAIDILKE QWTKIDQRSN DTKSSSTQDA LIKEIQDLEK 450
GFRELSDLTH KKYSEIINHE SVISKLTVEK TKADQKYFAA MRSKDSILIE 500
IKTLSKSLSK SNELILQLKD SDRLLQQKIG NLHKQLDLSQ NNERRLIDSS 550
KTETLKIIDL NNTSTKLKRS LEKLQEESNK SIADMTHLET KLNDTEIELK 600
HFKQKASHLE SKCEKLHDTL FRGNNKNKGS SDEALVEELA NFRTLVYCSL 650
CSKNWKNMAI KTCGHVFCEN CCKERLAARM RKCPTCNKAF SSNDLLTVHL 700
Length:700
Mass (Da):80,692
Last modified:November 1, 1996 - v1
Checksum:iFC6B32825970D36F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74122 Genomic DNA. Translation: CAA98640.1.
BK006938 Genomic DNA. Translation: DAA11785.1.
PIRiS67610.
RefSeqiNP_010209.1. NM_001180133.1.

Genome annotation databases

EnsemblFungiiYDL074C; YDL074C; YDL074C.
GeneIDi851485.
KEGGisce:YDL074C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74122 Genomic DNA. Translation: CAA98640.1 .
BK006938 Genomic DNA. Translation: DAA11785.1 .
PIRi S67610.
RefSeqi NP_010209.1. NM_001180133.1.

3D structure databases

ProteinModelPortali Q07457.
SMRi Q07457. Positions 647-672.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31987. 396 interactions.
DIPi DIP-2999N.
IntActi Q07457. 72 interactions.
MINTi MINT-434946.
STRINGi 4932.YDL074C.

Proteomic databases

MaxQBi Q07457.
PaxDbi Q07457.
PeptideAtlasi Q07457.
PRIDEi Q07457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL074C ; YDL074C ; YDL074C .
GeneIDi 851485.
KEGGi sce:YDL074C.

Organism-specific databases

CYGDi YDL074c.
SGDi S000002232. BRE1.

Phylogenomic databases

eggNOGi NOG263074.
GeneTreei ENSGT00390000002866.
HOGENOMi HOG000095255.
KOi K10696.
OMAi CENCCKE.
OrthoDBi EOG7J9W0C.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci YEAST:G3O-29485-MONOMER.

Miscellaneous databases

NextBioi 968807.
PROi Q07457.

Gene expression databases

Genevestigatori Q07457.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR013956. E3_ubiquit_lig_BRE1.
IPR007087. Znf_C2H2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF08647. BRE1. 1 hit.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
PROSITEi PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p."
    Wood A., Schneider J., Dover J., Johnston M., Shilatifard A.
    J. Biol. Chem. 278:34739-34742(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "A conserved RING finger protein required for histone H2B monoubiquitination and cell size control."
    Hwang W.W., Venkatasubrahmanyam S., Ianculescu A.G., Tong A., Boone C., Madhani H.D.
    Mol. Cell 11:261-266(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LGE1.
  5. "Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter."
    Wood A., Krogan N.J., Dover J., Schneider J., Heidt J., Boateng M.A., Dean K., Golshani A., Zhang Y., Greenblatt J.F., Johnston M., Shilatifard A.
    Mol. Cell 11:267-274(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-663 AND HIS-665.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Rad6-Bre1-mediated histone H2B ubiquitylation modulates the formation of double-strand breaks during meiosis."
    Yamashita K., Shinohara M., Shinohara A.
    Proc. Natl. Acad. Sci. U.S.A. 101:11380-11385(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Histone H2B ubiquitylation is associated with elongating RNA polymerase II."
    Xiao T., Kao C.-F., Krogan N.J., Sun Z.-W., Greenblatt J.F., Osley M.A., Strahl B.D.
    Mol. Cell. Biol. 25:637-651(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiBRE1_YEAST
AccessioniPrimary (citable) accession number: Q07457
Secondary accession number(s): D6VRS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2980 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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