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Q07457

- BRE1_YEAST

UniProt

Q07457 - BRE1_YEAST

Protein

E3 ubiquitin-protein ligase BRE1

Gene

BRE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2B to form H2BK123ub1 in association with the E2 enzyme RAD6/UBC2. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. It thereby plays a central role in histone code and gene regulation. Also modulates the formation of double-strand breaks during meiosis.5 Publications

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri648 – 68740RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA replication origin binding Source: SGD
    2. identical protein binding Source: IntAct
    3. ligase activity Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. ubiquitin-protein transferase activity Source: SGD
    6. zinc ion binding Source: InterPro

    GO - Biological processi

    1. chromatin silencing at telomere Source: SGD
    2. double-strand break repair via homologous recombination Source: SGD
    3. histone monoubiquitination Source: SGD
    4. histone ubiquitination Source: SGD
    5. intra-S DNA damage checkpoint Source: SGD
    6. meiotic DNA double-strand break formation Source: SGD
    7. mitotic G1 DNA damage checkpoint Source: SGD
    8. regulation of DNA-dependent DNA replication initiation Source: SGD
    9. telomere maintenance via recombination Source: SGD
    10. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Chromatin regulator, Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29485-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase BRE1 (EC:6.3.2.-)
    Alternative name(s):
    Brefeldin A-sensitivity protein 1
    Gene namesi
    Name:BRE1
    Ordered Locus Names:YDL074C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL074c.
    SGDiS000002232. BRE1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi663 – 6631C → A: Abolishes ability to monoubiquitinate histone H2B. 1 Publication
    Mutagenesisi665 – 6651H → A: Abolishes ability to monoubiquitinate histone H2B. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 700700E3 ubiquitin-protein ligase BRE1PRO_0000055857Add
    BLAST

    Proteomic databases

    MaxQBiQ07457.
    PaxDbiQ07457.
    PeptideAtlasiQ07457.
    PRIDEiQ07457.

    Expressioni

    Gene expression databases

    GenevestigatoriQ07457.

    Interactioni

    Subunit structurei

    Forms a complex with the E2 enzyme RAD6/UBC2. May interact with LGE1.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself6EBI-31563,EBI-31563
    RAD6P061044EBI-31563,EBI-19722

    Protein-protein interaction databases

    BioGridi31987. 397 interactions.
    DIPiDIP-2999N.
    IntActiQ07457. 72 interactions.
    MINTiMINT-434946.
    STRINGi4932.YDL074C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07457.
    SMRiQ07457. Positions 647-672.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili126 – 406281Sequence AnalysisAdd
    BLAST
    Coiled coili555 – 62066Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the BRE1 family.Curated
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri648 – 68740RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG263074.
    GeneTreeiENSGT00390000002866.
    HOGENOMiHOG000095255.
    KOiK10696.
    OMAiCENCCKE.
    OrthoDBiEOG7J9W0C.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR013956. E3_ubiquit_lig_BRE1.
    IPR007087. Znf_C2H2.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF08647. BRE1. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    PROSITEiPS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q07457-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAEPATKKI KLELSDPSEP LTQSDVIAFQ KEALFRCINR RRVDFEALRK    50
    QYELSRRECI DVSRKLANIM ALIVTLARFI ETFCTDANEK QLCREIAQGD 100
    ETLIVQRSDS FMKLLTKYGK PNTTDSNTNS NASDHIQELT TELKNLRKSK 150
    EELFYENSQL TEEISALKEY YTNIIRKYDR DESFTIKRVF KEDKTDAVKE 200
    LREDEKESNE NNIKSGNKDS SAINGDNTSK KSEKGDELVQ AEDERKEDAE 250
    NEKLELDLKF SDLRAEINSL SSTIKDLENI RRENEEELIK TRSEVSNLKK 300
    QQIAAADQDP DFKSYDHESL LAKIQHLTEQ NAELSEINSS FLSKFQVLAK 350
    EKEIYTKKVR EEFQKSLDSL VEMNSSLEKD VVRIRTARDD LLSKIAILEA 400
    EKSKTEVLSD LQHAIDILKE QWTKIDQRSN DTKSSSTQDA LIKEIQDLEK 450
    GFRELSDLTH KKYSEIINHE SVISKLTVEK TKADQKYFAA MRSKDSILIE 500
    IKTLSKSLSK SNELILQLKD SDRLLQQKIG NLHKQLDLSQ NNERRLIDSS 550
    KTETLKIIDL NNTSTKLKRS LEKLQEESNK SIADMTHLET KLNDTEIELK 600
    HFKQKASHLE SKCEKLHDTL FRGNNKNKGS SDEALVEELA NFRTLVYCSL 650
    CSKNWKNMAI KTCGHVFCEN CCKERLAARM RKCPTCNKAF SSNDLLTVHL 700
    Length:700
    Mass (Da):80,692
    Last modified:November 1, 1996 - v1
    Checksum:iFC6B32825970D36F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74122 Genomic DNA. Translation: CAA98640.1.
    BK006938 Genomic DNA. Translation: DAA11785.1.
    PIRiS67610.
    RefSeqiNP_010209.1. NM_001180133.1.

    Genome annotation databases

    EnsemblFungiiYDL074C; YDL074C; YDL074C.
    GeneIDi851485.
    KEGGisce:YDL074C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74122 Genomic DNA. Translation: CAA98640.1 .
    BK006938 Genomic DNA. Translation: DAA11785.1 .
    PIRi S67610.
    RefSeqi NP_010209.1. NM_001180133.1.

    3D structure databases

    ProteinModelPortali Q07457.
    SMRi Q07457. Positions 647-672.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31987. 397 interactions.
    DIPi DIP-2999N.
    IntActi Q07457. 72 interactions.
    MINTi MINT-434946.
    STRINGi 4932.YDL074C.

    Proteomic databases

    MaxQBi Q07457.
    PaxDbi Q07457.
    PeptideAtlasi Q07457.
    PRIDEi Q07457.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL074C ; YDL074C ; YDL074C .
    GeneIDi 851485.
    KEGGi sce:YDL074C.

    Organism-specific databases

    CYGDi YDL074c.
    SGDi S000002232. BRE1.

    Phylogenomic databases

    eggNOGi NOG263074.
    GeneTreei ENSGT00390000002866.
    HOGENOMi HOG000095255.
    KOi K10696.
    OMAi CENCCKE.
    OrthoDBi EOG7J9W0C.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-29485-MONOMER.

    Miscellaneous databases

    NextBioi 968807.
    PROi Q07457.

    Gene expression databases

    Genevestigatori Q07457.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR013956. E3_ubiquit_lig_BRE1.
    IPR007087. Znf_C2H2.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF08647. BRE1. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    PROSITEi PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p."
      Wood A., Schneider J., Dover J., Johnston M., Shilatifard A.
      J. Biol. Chem. 278:34739-34742(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "A conserved RING finger protein required for histone H2B monoubiquitination and cell size control."
      Hwang W.W., Venkatasubrahmanyam S., Ianculescu A.G., Tong A., Boone C., Madhani H.D.
      Mol. Cell 11:261-266(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH LGE1.
    5. "Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter."
      Wood A., Krogan N.J., Dover J., Schneider J., Heidt J., Boateng M.A., Dean K., Golshani A., Zhang Y., Greenblatt J.F., Johnston M., Shilatifard A.
      Mol. Cell 11:267-274(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-663 AND HIS-665.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Rad6-Bre1-mediated histone H2B ubiquitylation modulates the formation of double-strand breaks during meiosis."
      Yamashita K., Shinohara M., Shinohara A.
      Proc. Natl. Acad. Sci. U.S.A. 101:11380-11385(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Histone H2B ubiquitylation is associated with elongating RNA polymerase II."
      Xiao T., Kao C.-F., Krogan N.J., Sun Z.-W., Greenblatt J.F., Osley M.A., Strahl B.D.
      Mol. Cell. Biol. 25:637-651(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiBRE1_YEAST
    AccessioniPrimary (citable) accession number: Q07457
    Secondary accession number(s): D6VRS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2980 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3