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Protein

E3 ubiquitin-protein ligase BRE1

Gene

BRE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2B to form H2BK123ub1 in association with the E2 enzyme RAD6/UBC2. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. It thereby plays a central role in histone code and gene regulation. Also modulates the formation of double-strand breaks during meiosis.5 Publications

Miscellaneous

Present with 2980 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri648 – 687RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • DNA replication origin binding Source: SGD
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • chromatin silencing at telomere Source: SGD
  • double-strand break repair via homologous recombination Source: SGD
  • histone monoubiquitination Source: SGD
  • histone ubiquitination Source: SGD
  • intra-S DNA damage checkpoint Source: SGD
  • meiotic DNA double-strand break formation Source: SGD
  • mitotic G1 DNA damage checkpoint Source: SGD
  • protein K63-linked ubiquitination Source: SGD
  • regulation of DNA-dependent DNA replication initiation Source: SGD
  • telomere maintenance via recombination Source: SGD
  • transcription by RNA polymerase II Source: SGD

Keywordsi

Molecular functionChromatin regulator, Transferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29485-MONOMER
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1 (EC:2.3.2.271 Publication)
Alternative name(s):
Brefeldin A-sensitivity protein 1
RING-type E3 ubiquitin transferase BRE1Curated
Gene namesi
Name:BRE1
Ordered Locus Names:YDL074C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL074C
SGDiS000002232 BRE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi663C → A: Abolishes ability to monoubiquitinate histone H2B. 1 Publication1
Mutagenesisi665H → A: Abolishes ability to monoubiquitinate histone H2B. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558571 – 700E3 ubiquitin-protein ligase BRE1Add BLAST700

Proteomic databases

MaxQBiQ07457
PaxDbiQ07457
PRIDEiQ07457

PTM databases

iPTMnetiQ07457

Interactioni

Subunit structurei

Forms a complex with the E2 enzyme RAD6/UBC2. May interact with LGE1.

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi31987, 759 interactors
DIPiDIP-2999N
IntActiQ07457, 93 interactors
MINTiQ07457
STRINGi4932.YDL074C

Structurei

Secondary structure

1700
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi633 – 646Combined sources14
Turni649 – 651Combined sources3
Beta strandi652 – 655Combined sources4
Beta strandi658 – 660Combined sources3
Turni661 – 663Combined sources3
Helixi669 – 677Combined sources9
Turni684 – 686Combined sources3
Helixi692 – 694Combined sources3
Beta strandi695 – 697Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4R7EX-ray2.25A632-700[»]
ProteinModelPortaliQ07457
SMRiQ07457
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili126 – 406Sequence analysisAdd BLAST281
Coiled coili555 – 620Sequence analysisAdd BLAST66

Sequence similaritiesi

Belongs to the BRE1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri648 – 687RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000002866
HOGENOMiHOG000095255
InParanoidiQ07457
KOiK10696
OMAiAIKTCGH
OrthoDBiEOG092C0ULK

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR013956 E3_ubiquit_lig_Bre1
IPR013087 Znf_C2H2_type
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR23163 PTHR23163, 2 hits
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q07457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAEPATKKI KLELSDPSEP LTQSDVIAFQ KEALFRCINR RRVDFEALRK
60 70 80 90 100
QYELSRRECI DVSRKLANIM ALIVTLARFI ETFCTDANEK QLCREIAQGD
110 120 130 140 150
ETLIVQRSDS FMKLLTKYGK PNTTDSNTNS NASDHIQELT TELKNLRKSK
160 170 180 190 200
EELFYENSQL TEEISALKEY YTNIIRKYDR DESFTIKRVF KEDKTDAVKE
210 220 230 240 250
LREDEKESNE NNIKSGNKDS SAINGDNTSK KSEKGDELVQ AEDERKEDAE
260 270 280 290 300
NEKLELDLKF SDLRAEINSL SSTIKDLENI RRENEEELIK TRSEVSNLKK
310 320 330 340 350
QQIAAADQDP DFKSYDHESL LAKIQHLTEQ NAELSEINSS FLSKFQVLAK
360 370 380 390 400
EKEIYTKKVR EEFQKSLDSL VEMNSSLEKD VVRIRTARDD LLSKIAILEA
410 420 430 440 450
EKSKTEVLSD LQHAIDILKE QWTKIDQRSN DTKSSSTQDA LIKEIQDLEK
460 470 480 490 500
GFRELSDLTH KKYSEIINHE SVISKLTVEK TKADQKYFAA MRSKDSILIE
510 520 530 540 550
IKTLSKSLSK SNELILQLKD SDRLLQQKIG NLHKQLDLSQ NNERRLIDSS
560 570 580 590 600
KTETLKIIDL NNTSTKLKRS LEKLQEESNK SIADMTHLET KLNDTEIELK
610 620 630 640 650
HFKQKASHLE SKCEKLHDTL FRGNNKNKGS SDEALVEELA NFRTLVYCSL
660 670 680 690 700
CSKNWKNMAI KTCGHVFCEN CCKERLAARM RKCPTCNKAF SSNDLLTVHL
Length:700
Mass (Da):80,692
Last modified:November 1, 1996 - v1
Checksum:iFC6B32825970D36F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74122 Genomic DNA Translation: CAA98640.1
BK006938 Genomic DNA Translation: DAA11785.1
PIRiS67610
RefSeqiNP_010209.1, NM_001180133.1

Genome annotation databases

EnsemblFungiiYDL074C; YDL074C; YDL074C
GeneIDi851485
KEGGisce:YDL074C

Similar proteinsi

Entry informationi

Entry nameiBRE1_YEAST
AccessioniPrimary (citable) accession number: Q07457
Secondary accession number(s): D6VRS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health