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Q07456

- AMBP_MOUSE

UniProt

Q07456 - AMBP_MOUSE

Protein

Protein AMBP

Gene

Ambp

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization By similarity.By similarity
    Trypstatin is a trypsin inhibitor.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521Multimeric 3-hydroxykynurenine chromophore (covalent)By similarity
    Binding sitei110 – 1101Multimeric 3-hydroxykynurenine chromophore (covalent)By similarity
    Binding sitei136 – 1361Multimeric 3-hydroxykynurenine chromophore (covalent)By similarity
    Binding sitei148 – 1481Multimeric 3-hydroxykynurenine chromophore (covalent)By similarity
    Sitei240 – 2412Inhibitory (P1) (chymotrypsin, elastase)By similarity
    Sitei296 – 2972Inhibitory (P1) (trypsin)By similarity

    GO - Molecular functioni

    1. heme binding Source: UniProtKB
    2. IgA binding Source: UniProtKB
    3. protein homodimerization activity Source: UniProtKB
    4. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
    5. small molecule binding Source: InterPro

    GO - Biological processi

    1. protein catabolic process Source: Ensembl
    2. protein-chromophore linkage Source: UniProtKB-KW
    3. protein maturation Source: MGI

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Keywords - Ligandi

    Chromophore

    Enzyme and pathway databases

    ReactomeiREACT_196575. Scavenging of heme from plasma.

    Protein family/group databases

    MEROPSiI02.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein AMBP
    Cleaved into the following 3 chains:
    Alternative name(s):
    Bikunin
    HI-30
    Gene namesi
    Name:Ambp
    Synonyms:Itil
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:88002. Ambp.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: Ensembl
    2. extracellular space Source: Ensembl
    3. intracellular membrane-bounded organelle Source: Ensembl
    4. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919By similarityAdd
    BLAST
    Chaini20 – 202183Alpha-1-microglobulinPRO_0000017893Add
    BLAST
    Chaini205 – 349145Inter-alpha-trypsin inhibitor light chainPRO_0000017894Add
    BLAST
    Chaini283 – 34361TrypstatinBy similarityPRO_0000318928Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi90 ↔ 187PROSITE-ProRule annotation
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi230 ↔ 280PROSITE-ProRule annotation
    Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi239 ↔ 263PROSITE-ProRule annotation
    Disulfide bondi255 ↔ 276PROSITE-ProRule annotation
    Disulfide bondi286 ↔ 336PROSITE-ProRule annotation
    Disulfide bondi295 ↔ 319PROSITE-ProRule annotation
    Disulfide bondi311 ↔ 332PROSITE-ProRule annotation

    Post-translational modificationi

    The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-52, Lys-110, Lys-136, and Lys-148 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region By similarity.By similarity
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ07456.
    PaxDbiQ07456.
    PRIDEiQ07456.

    PTM databases

    PhosphoSiteiQ07456.

    Expressioni

    Tissue specificityi

    Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.

    Gene expression databases

    ArrayExpressiQ07456.
    BgeeiQ07456.
    CleanExiMM_AMBP.
    GenevestigatoriQ07456.

    Interactioni

    Subunit structurei

    I-alpha-I plasma protease inhibitors are assembled from one or two heavy chains (H1, H2 or H3) and one light chain, bikunin. Inter-alpha-inhibitor (I-alpha-I) is composed of H1, H2 and bikunin, inter-alpha-like inhibitor (I-alpha-LI) of H2 and bikunin, and pre-alpha-inhibitor (P-alpha-I) of H3 and bikunin. Alpha-1-microglobulin occurs as a monomer and also in complexes with IgA and albumin. Alpha-1-microglobulin interacts with FN1. Trypstatin is a monomer and also occurs as a complex with tryptase in mast cells By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ07456.
    SMRiQ07456. Positions 27-192, 229-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini230 – 28051BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini286 – 33651BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.Curated
    Contains 2 BPTI/Kunitz inhibitor domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG328322.
    GeneTreeiENSGT00740000114929.
    HOGENOMiHOG000001572.
    HOVERGENiHBG000225.
    InParanoidiQ07456.
    OMAiGTSMACE.
    OrthoDBiEOG73FQKT.
    PhylomeDBiQ07456.
    TreeFamiTF351222.

    Family and domain databases

    Gene3Di2.40.128.20. 1 hit.
    4.10.410.10. 2 hits.
    InterProiIPR002968. A1-microglobln.
    IPR012674. Calycin.
    IPR011038. Calycin-like.
    IPR002345. Lipocalin.
    IPR022272. Lipocalin_CS.
    IPR000566. Lipocln_cytosolic_FA-bd_dom.
    IPR002223. Prot_inh_Kunz-m.
    IPR020901. Prtase_inh_Kunz-CS.
    [Graphical view]
    PfamiPF00014. Kunitz_BPTI. 2 hits.
    PF00061. Lipocalin. 1 hit.
    [Graphical view]
    PRINTSiPR01215. A1MCGLOBULIN.
    PR00759. BASICPTASE.
    PR00179. LIPOCALIN.
    SMARTiSM00131. KU. 2 hits.
    [Graphical view]
    SUPFAMiSSF50814. SSF50814. 1 hit.
    SSF57362. SSF57362. 2 hits.
    PROSITEiPS00280. BPTI_KUNITZ_1. 2 hits.
    PS50279. BPTI_KUNITZ_2. 2 hits.
    PS00213. LIPOCALIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q07456-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGLRTLFLL LTACLASRAD PASTLPDIQV QENFSESRIY GKWYNLAVGS    50
    TCPWLSRIKD KMSVSTLVLQ EGATETEISM TSTRWRRGVC EEITGAYQKT 100
    DIDGKFLYHK SKWNITLESY VVHTNYDEYA IFLTKKSSHH HGLTITAKLY 150
    GREPQLRDSL LQEFKDVALN VGISENSIIF MPDRGECVPG DREVEPTSIA 200
    RARRAVLPQE SEGSGTEPLI TGTLKKEDSC QLNYSEGPCL GMQERYYYNG 250
    ASMACETFQY GGCLGNGNNF ISEKDCLQTC RTIAACNLPI VQGPCRAFIK 300
    LWAFDAAQGK CIQFHYGGCK GNGNKFYSEK ECKEYCGVPG DGYEELIRS 349
    Length:349
    Mass (Da):39,029
    Last modified:February 26, 2008 - v2
    Checksum:iCFB9208D37DF0021
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651S → Q in CAA48640. (PubMed:7689339)Curated
    Sequence conflicti185 – 1851G → E in BAB23659. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68680 mRNA. Translation: CAA48640.1.
    D28812 mRNA. Translation: BAA05973.1.
    AF034692 Genomic DNA. Translation: AAD01995.1.
    AK004907 mRNA. Translation: BAB23659.1.
    BC021660 mRNA. Translation: AAH21660.1.
    CCDSiCCDS18248.1.
    PIRiS35708.
    RefSeqiNP_031469.1. NM_007443.3.
    UniGeneiMm.2197.

    Genome annotation databases

    EnsembliENSMUST00000030041; ENSMUSP00000030041; ENSMUSG00000028356.
    GeneIDi11699.
    KEGGimmu:11699.
    UCSCiuc008tfp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68680 mRNA. Translation: CAA48640.1 .
    D28812 mRNA. Translation: BAA05973.1 .
    AF034692 Genomic DNA. Translation: AAD01995.1 .
    AK004907 mRNA. Translation: BAB23659.1 .
    BC021660 mRNA. Translation: AAH21660.1 .
    CCDSi CCDS18248.1.
    PIRi S35708.
    RefSeqi NP_031469.1. NM_007443.3.
    UniGenei Mm.2197.

    3D structure databases

    ProteinModelPortali Q07456.
    SMRi Q07456. Positions 27-192, 229-338.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi I02.006.

    PTM databases

    PhosphoSitei Q07456.

    Proteomic databases

    MaxQBi Q07456.
    PaxDbi Q07456.
    PRIDEi Q07456.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030041 ; ENSMUSP00000030041 ; ENSMUSG00000028356 .
    GeneIDi 11699.
    KEGGi mmu:11699.
    UCSCi uc008tfp.1. mouse.

    Organism-specific databases

    CTDi 259.
    MGIi MGI:88002. Ambp.

    Phylogenomic databases

    eggNOGi NOG328322.
    GeneTreei ENSGT00740000114929.
    HOGENOMi HOG000001572.
    HOVERGENi HBG000225.
    InParanoidi Q07456.
    OMAi GTSMACE.
    OrthoDBi EOG73FQKT.
    PhylomeDBi Q07456.
    TreeFami TF351222.

    Enzyme and pathway databases

    Reactomei REACT_196575. Scavenging of heme from plasma.

    Miscellaneous databases

    NextBioi 279367.
    PROi Q07456.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q07456.
    Bgeei Q07456.
    CleanExi MM_AMBP.
    Genevestigatori Q07456.

    Family and domain databases

    Gene3Di 2.40.128.20. 1 hit.
    4.10.410.10. 2 hits.
    InterProi IPR002968. A1-microglobln.
    IPR012674. Calycin.
    IPR011038. Calycin-like.
    IPR002345. Lipocalin.
    IPR022272. Lipocalin_CS.
    IPR000566. Lipocln_cytosolic_FA-bd_dom.
    IPR002223. Prot_inh_Kunz-m.
    IPR020901. Prtase_inh_Kunz-CS.
    [Graphical view ]
    Pfami PF00014. Kunitz_BPTI. 2 hits.
    PF00061. Lipocalin. 1 hit.
    [Graphical view ]
    PRINTSi PR01215. A1MCGLOBULIN.
    PR00759. BASICPTASE.
    PR00179. LIPOCALIN.
    SMARTi SM00131. KU. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50814. SSF50814. 1 hit.
    SSF57362. SSF57362. 2 hits.
    PROSITEi PS00280. BPTI_KUNITZ_1. 2 hits.
    PS50279. BPTI_KUNITZ_2. 2 hits.
    PS00213. LIPOCALIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse alpha-1-microglobulin/bikunin precursor: cDNA analysis, gene evolution and physical assignment of the gene next to the orosomucoid locus."
      Chan P., Salier J.-P.
      Biochim. Biophys. Acta 1174:195-200(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Liver.
    2. "cDNA sequencing of mouse alpha 1-microglobulin/inter-alpha-trypsin inhibitor light chain and its expression in acute inflammation."
      Itoh H., Ide H., Kataoka H., Tomita M., Yoshihara H., Nawa Y.
      J. Biochem. 116:767-772(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Liver.
    3. "The alpha1-microglobulin/bikunin gene: characterization in mouse and evolution."
      Lindqvist A., Rouet P., Salier J.-P., Aakerstroem B.
      Gene 234:329-336(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Liver.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.

    Entry informationi

    Entry nameiAMBP_MOUSE
    AccessioniPrimary (citable) accession number: Q07456
    Secondary accession number(s): Q61294, Q925W1, Q9DBJ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3