Reviewed, UniProtKB/Swiss-Prot Q07448 (S3AD_ENTFA)

Names and origin

Protein names

Recommended name:
    Streptomycin 3''-adenylyltransferase
    EC=2.7.7.47
Alternative name(s):
    AAD(9)

Gene names

Name:	spc
Synonyms:	aad9

Encoded on

Plasmid pDL55

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Mediates bacterial resistance to the antibiotics streptomycin and spectomycin.
Catalytic activity	ATP + streptomycin = diphosphate + 3''-adenylylstreptomycin.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoglycoside 3''-adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Streptomycin 3''-adenylyltransferase		PRO_0000068582

Sequences

	Sequence		Length	Mass (Da)
	Q07448-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 367505B0CA0E68B4	FASTA	255	29,821
	10 20 30 40 50 60 MRRIYLNTYE QINKVKKILR KHLKNNLIGT YMFGSGVESG LKPNSDLDFL VVVSEPLTDQ 70 80 90 100 110 120 SKEILIQKIR PISKKIGDKS NLRYIELTII IQQEMVPWNH PPKQEFIYGE WLQELYEQGY 130 140 150 160 170 180 IPQKELNSDL TIMLYQAKRK NKRIYGNYDL EELLPDIPFS DVRRAIMDSS EELIDNYQDD 190 200 210 220 230 240 ETNSILTLCR MILTMDTGKI IPKDIAGNAV AESSPLEHRE RILLAVRSYL GENIEWTNEN 250 VNLTINYLNN RLKKL

References

[1]

"Cloning and nucleotide base sequence analysis of a spectinomycin adenyltransferase AAD(9) determinant from Enterococcus faecalis." Leblanc D.J., Lee L.N., Inamine J.M. Antimicrob. Agents Chemother. 35:1804-1810(1991) [PubMed: 1659306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LDR55.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M69221 Unassigned DNA. Translation: AAA16527.1.
PIR	A61153.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.47. 704.
Family and domain databases
InterPro	IPR002934. Nucleotidyltransferase. [Graphical view]
Pfam	PF01909. NTP_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

S3AD_ENTFA

Accession

Primary (citable) accession number: Q07448

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)